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Protein

Tryptophan synthase beta chain

Gene

trpB

Organism
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine.By similarity

Catalytic activityi

L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Cofactori

Pathway:iL-tryptophan biosynthesis

This protein is involved in step 5 of the subpathway that synthesizes L-tryptophan from chorismate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Anthranilate synthase component 2 (trpG), Anthranilate synthase component 1 (trpE)
  2. Anthranilate phosphoribosyltransferase (trpD)
  3. Tryptophan biosynthesis protein TrpCF (trpC)
  4. Tryptophan biosynthesis protein TrpCF (trpC)
  5. Tryptophan synthase alpha chain (trpA), Tryptophan synthase beta chain (trpB)
This subpathway is part of the pathway L-tryptophan biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-tryptophan from chorismate, the pathway L-tryptophan biosynthesis and in Amino-acid biosynthesis.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciBAPH198804:GHMG-281-MONOMER.
UniPathwayiUPA00035; UER00044.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan synthase beta chain (EC:4.2.1.20)
Gene namesi
Name:trpB
Ordered Locus Names:BUsg_267
OrganismiBuchnera aphidicola subsp. Schizaphis graminum (strain Sg)
Taxonomic identifieri198804 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeBuchnera
ProteomesiUP000000416 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 399399Tryptophan synthase beta chainPRO_0000098927Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei86 – 861N6-(pyridoxal phosphate)lysineBy similarity

Proteomic databases

PRIDEiP42391.

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains.By similarity

Protein-protein interaction databases

STRINGi198804.BUsg267.

Structurei

3D structure databases

ProteinModelPortaliP42391.
SMRiP42391. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TrpB family.Curated

Phylogenomic databases

eggNOGiCOG0133.
KOiK01696.
OMAiIPEMLYP.
OrthoDBiEOG6GFGH7.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PANTHERiPTHR10314:SF3. PTHR10314:SF3. 1 hit.
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42391-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLLNPYFGK FGGMYVPQIL MPALYQLEKN FVDAKKDSNF QKSFFNYLKN
60 70 80 90 100
YAGRPTPLTL CNNLTNGTKT RIYLKREDLL HGGAHKTNQV LGQAMLAVKM
110 120 130 140 150
KKKEIIAETG AGQHGVAAAI ASALFNLKCK IYMGYKDIKR QSPNVFRMKL
160 170 180 190 200
MGAEVVSVES GSGTLKDACN EALRDWSRNY QKSHYMIGTA AGPHPYPTIV
210 220 230 240 250
KEFQKMIGEE AKKQILEQEN RLPDAIIACV GGGSNAIGIF SDFIDEDVNL
260 270 280 290 300
IGVEPAGQGI ETGKHGAPLN HGRTGIYFGM KSHLMQSQEG QIEKSWSISA
310 320 330 340 350
GLDFPSVGPE HSWLNSIHRA KYVSITDIEA LEAFQILSKK EGIIPALESS
360 370 380 390
HALAYALKLM YLDPKKEQVF IVNLSGRGDK DIFTVREILK KTEKKHESL
Length:399
Mass (Da):44,247
Last modified:November 1, 1995 - v1
Checksum:iDEBFD45D2C859C30
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19055 Genomic DNA. Translation: CAA79500.1.
AE013218 Genomic DNA. Translation: AAM67825.1.
PIRiC49897.
RefSeqiWP_011053792.1. NC_004061.1.

Genome annotation databases

EnsemblBacteriaiAAM67825; AAM67825; BUsg_267.
KEGGibas:BUsg267.
PATRICi21247339. VBIBucAph100086_0279.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z19055 Genomic DNA. Translation: CAA79500.1.
AE013218 Genomic DNA. Translation: AAM67825.1.
PIRiC49897.
RefSeqiWP_011053792.1. NC_004061.1.

3D structure databases

ProteinModelPortaliP42391.
SMRiP42391. Positions 2-390.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198804.BUsg267.

Proteomic databases

PRIDEiP42391.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAM67825; AAM67825; BUsg_267.
KEGGibas:BUsg267.
PATRICi21247339. VBIBucAph100086_0279.

Phylogenomic databases

eggNOGiCOG0133.
KOiK01696.
OMAiIPEMLYP.
OrthoDBiEOG6GFGH7.

Enzyme and pathway databases

UniPathwayiUPA00035; UER00044.
BioCyciBAPH198804:GHMG-281-MONOMER.

Family and domain databases

HAMAPiMF_00133. Trp_synth_beta.
InterProiIPR006653. Trp_synth_b_CS.
IPR006654. Trp_synth_beta.
IPR023026. Trp_synth_beta/beta-like.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PANTHERiPTHR10314:SF3. PTHR10314:SF3. 1 hit.
PfamiPF00291. PALP. 1 hit.
[Graphical view]
PIRSFiPIRSF001413. Trp_syn_beta. 1 hit.
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR00263. trpB. 1 hit.
PROSITEiPS00168. TRP_SYNTHASE_BETA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and nucleotide sequence of a putative trpDC(F)BA operon in Buchnera aphidicola (endosymbiont of the aphid Schizaphis graminum)."
    Munson M.A., Baumann P.
    J. Bacteriol. 175:6426-6432(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Sg.

Entry informationi

Entry nameiTRPB_BUCAP
AccessioniPrimary (citable) accession number: P42391
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 22, 2015
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Buchnera aphidicola (subsp. Schizaphis graminum)
    Buchnera aphidicola (subsp. Schizaphis graminum): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.