P42391 (TRPB_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tryptophan synthase beta chain
EC=4.2.1.20

Gene names

Name:	trpB
Ordered Locus Names:	BUsg_267

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera › Buchnera aphidicola › Buchnera aphidicola (Schizaphis graminum)

Protein attributes

Sequence length	399 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The beta subunit is responsible for the synthesis of L-tryptophan from indole and L-serine By similarity. HAMAP-Rule MF_00133
Catalytic activity	L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + glyceraldehyde 3-phosphate + H2O. HAMAP-Rule MF_00133
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_00133
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 5/5. HAMAP-Rule MF_00133
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Sequence similarities	Belongs to the TrpB family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular_function	tryptophan synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 399	399	Tryptophan synthase beta chain HAMAP-Rule MF_00133		PRO_0000098927
Amino acid modifications
	Modified residue	86	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42391 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: DEBFD45D2C859C30	FASTA	399	44,247
	10 20 30 40 50 60 MTLLNPYFGK FGGMYVPQIL MPALYQLEKN FVDAKKDSNF QKSFFNYLKN YAGRPTPLTL 70 80 90 100 110 120 CNNLTNGTKT RIYLKREDLL HGGAHKTNQV LGQAMLAVKM KKKEIIAETG AGQHGVAAAI 130 140 150 160 170 180 ASALFNLKCK IYMGYKDIKR QSPNVFRMKL MGAEVVSVES GSGTLKDACN EALRDWSRNY 190 200 210 220 230 240 QKSHYMIGTA AGPHPYPTIV KEFQKMIGEE AKKQILEQEN RLPDAIIACV GGGSNAIGIF 250 260 270 280 290 300 SDFIDEDVNL IGVEPAGQGI ETGKHGAPLN HGRTGIYFGM KSHLMQSQEG QIEKSWSISA 310 320 330 340 350 360 GLDFPSVGPE HSWLNSIHRA KYVSITDIEA LEAFQILSKK EGIIPALESS HALAYALKLM 370 380 390 YLDPKKEQVF IVNLSGRGDK DIFTVREILK KTEKKHESL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and nucleotide sequence of a putative trpDC(F)BA operon in Buchnera aphidicola (endosymbiont of the aphid Schizaphis graminum)." Munson M.A., Baumann P. J. Bacteriol. 175:6426-6432(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"50 million years of genomic stasis in endosymbiotic bacteria." Tamas I., Klasson L., Canbaeck B., Naeslund A.K., Eriksson A.-S., Wernegreen J.J., Sandstroem J.P., Moran N.A., Andersson S.G.E. Science 296:2376-2379(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z19055 Genomic DNA. Translation: CAA79500.1. AE013218 Genomic DNA. Translation: AAM67825.1.
PIR	C49897.
RefSeq	NP_660614.1. NC_004061.1.
3D structure databases
ProteinModelPortal	P42391.
SMR	P42391. Positions 2-390.
ModBase	Search...
Protein-protein interaction databases
STRING	198804.BUsg267.
Proteomic databases
PRIDE	P42391.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAM67825; AAM67825; BUsg_267.
GeneID	1005469.
KEGG	bas:BUsg267.
PATRIC	21247339. VBIBucAph100086_0279.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0133.
KO	K01696.
OMA	REDLCHT.
ProtClustDB	PRK04346.
Enzyme and pathway databases
BioCyc	BAPH198804:GHMG-281-MONOMER.
UniPathway	UPA00035; UER00044.
Family and domain databases
HAMAP	MF_00133. Trp_synth_beta.
InterPro	IPR001926. Trp_syn_b_sub_like_PLP_eny_SF. IPR006653. Trp_synth_b_CS. IPR006654. Trp_synth_beta. IPR023026. Trp_synth_beta/beta-like. [Graphical view]
PANTHER	PTHR10314:SF3. PTHR10314:SF3. 1 hit.
Pfam	PF00291. PALP. 1 hit. [Graphical view]
PIRSF	PIRSF001413. Trp_syn_beta. 1 hit.
SUPFAM	SSF53686. PyrdxlP-dep_enz_bsu. 1 hit.
TIGRFAMs	TIGR00263. trpB. 1 hit.
PROSITE	PS00168. TRP_SYNTHASE_BETA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPB_BUCAP

Accession

Primary (citable) accession number: P42391

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 29, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program