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P42390

- TRPA_MAIZE

UniProt

P42390 - TRPA_MAIZE

Protein

Indole-3-glycerol phosphate lyase, chloroplastic

Gene

BX1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 2 (30 Aug 2005)
      Previous versions | rss
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    Functioni

    The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. In bacteria, tryptophan synthase alpha (TSA) activity is almost completely dependent on formation of an active alpha2beta2 complex with tryptophan synthase beta (TSB), and indole is usually not released during tryptophan synthesis. In maize, the TSA homolog BX1 catalyzes the formation of free indole from indole-3-glycerol phosphate, independently of TSB.1 Publication

    Catalytic activityi

    (1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate.
    L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

    Kineticsi

    1. KM=0.013 mM for indole-3-glycerol phosphate1 Publication

    Pathwayi

    GO - Molecular functioni

    1. indole-3-glycerol-phosphate lyase activity Source: UniProtKB-EC
    2. tryptophan synthase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-10162.
    UniPathwayiUPA00035; UER00044.
    UPA00872; UER00847.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Indole-3-glycerol phosphate lyase, chloroplastic (EC:4.1.2.8)
    Alternative name(s):
    Benzoxazineless 1
    Indole synthase
    Tryptophan synthase alpha chain (EC:4.2.1.20)
    Gene namesi
    Name:BX1
    OrganismiZea mays (Maize)
    Taxonomic identifieri4577 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

    Organism-specific databases

    GrameneiP42390.
    MaizeGDBi102199.

    Subcellular locationi

    Plastidchloroplast Curated

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5353ChloroplastSequence AnalysisAdd
    BLAST
    Chaini54 – 347294Indole-3-glycerol phosphate lyase, chloroplasticPRO_0000035782Add
    BLAST

    Interactioni

    Subunit structurei

    Tetramer of two alpha and two beta chains for the tryptophan synthase activity. Homodimer of alpha chains for the indole-3-glycerol phosphate lyase activity.1 Publication

    Structurei

    Secondary structure

    1
    347
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi90 – 9910
    Beta strandi104 – 1107
    Helixi116 – 12813
    Beta strandi132 – 1376
    Helixi148 – 15811
    Turni159 – 1613
    Helixi164 – 17411
    Helixi175 – 1773
    Beta strandi182 – 1854
    Helixi189 – 1924
    Helixi197 – 2015
    Beta strandi206 – 2083
    Turni214 – 2163
    Helixi217 – 22610
    Beta strandi233 – 2353
    Helixi241 – 25010
    Beta strandi255 – 2584
    Helixi273 – 28412
    Beta strandi289 – 2935
    Helixi298 – 3069
    Beta strandi310 – 3145
    Helixi316 – 3238
    Beta strandi324 – 3274
    Helixi328 – 34619

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1RD5X-ray2.02A/B87-347[»]
    1TJRX-ray2.30A/B87-347[»]
    ProteinModelPortaliP42390.
    SMRiP42390. Positions 87-347.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP42390.

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi47 – 515Poly-Thr
    Compositional biasi52 – 12271Ala-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the TrpA family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOGENOMiHOG000223816.
    KOiK13222.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00131. Trp_synth_alpha.
    InterProiIPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view]
    PfamiPF00290. Trp_syntA. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    TIGRFAMsiTIGR00262. trpA. 1 hit.
    PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42390-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAFAPKTSSS SSLSSALQAA QSPPLLLRRM SSTATPRRRY DAAVVVTTTT    50
    TARAAAAAVT VPAAPPQAPA PAPVPPKQAA APAERRSRPV SDTMAALMAK 100
    GKTAFIPYIT AGDPDLATTA EALRLLDGCG ADVIELGVPC SDPYIDGPII 150
    QASVARALAS GTTMDAVLEM LREVTPELSC PVVLLSYYKP IMSRSLAEMK 200
    EAGVHGLIVP DLPYVAAHSL WSEAKNNNLE LVLLTTPAIP EDRMKEITKA 250
    SEGFVYLVSV NGVTGPRANV NPRVESLIQE VKKVTNKPVA VGFGISKPEH 300
    VKQIAQWGAD GVIIGSAMVR QLGEAASPKQ GLRRLEEYAR GMKNALP 347
    Length:347
    Mass (Da):36,521
    Last modified:August 30, 2005 - v2
    Checksum:i110D4215101F2012
    GO

    Sequence cautioni

    The sequence CAA54131.1 differs from that shown. Reason: Frameshift at positions 69 and 84.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76713 Genomic DNA. Translation: CAA54131.1. Frameshift.
    AY254103 mRNA. Translation: AAP33667.1.
    AY254104 mRNA. Translation: AAP33668.1.
    PIRiS56665.
    RefSeqiNP_001105219.1. NM_001111749.1.
    UniGeneiZm.283.

    Genome annotation databases

    GeneIDi542117.
    KEGGizma:542117.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X76713 Genomic DNA. Translation: CAA54131.1 . Frameshift.
    AY254103 mRNA. Translation: AAP33667.1 .
    AY254104 mRNA. Translation: AAP33668.1 .
    PIRi S56665.
    RefSeqi NP_001105219.1. NM_001111749.1.
    UniGenei Zm.283.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1RD5 X-ray 2.02 A/B 87-347 [» ]
    1TJR X-ray 2.30 A/B 87-347 [» ]
    ProteinModelPortali P42390.
    SMRi P42390. Positions 87-347.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 542117.
    KEGGi zma:542117.

    Organism-specific databases

    Gramenei P42390.
    MaizeGDBi 102199.

    Phylogenomic databases

    HOGENOMi HOG000223816.
    KOi K13222.

    Enzyme and pathway databases

    UniPathwayi UPA00035 ; UER00044 .
    UPA00872 ; UER00847 .
    BioCyci MetaCyc:MONOMER-10162.

    Miscellaneous databases

    EvolutionaryTracei P42390.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00131. Trp_synth_alpha.
    InterProi IPR013785. Aldolase_TIM.
    IPR011060. RibuloseP-bd_barrel.
    IPR018204. Trp_synthase_alpha_AS.
    IPR002028. Trp_synthase_suA.
    [Graphical view ]
    Pfami PF00290. Trp_syntA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    TIGRFAMsi TIGR00262. trpA. 1 hit.
    PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of a maize tryptophan synthase alpha subunit gene with pith enhanced expression."
      Kramer V.C., Koziel M.G.
      Plant Mol. Biol. 27:1183-1188(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: cv. CG000237.
    2. "Transformation of Nicotiana tabacum cv. Samsun with melanin and indigo genes."
      Jordaan A., Botha A.-M.
      Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
    4. "Crystal structure of tryptophan synthase alpha chain."
      Kulik V., Weyand M., Frey M., Dunn M., Schlichting I.
      Submitted (DEC-2004) to the PDB data bank
      Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 86-347, SUBUNIT.

    Entry informationi

    Entry nameiTRPA_MAIZE
    AccessioniPrimary (citable) accession number: P42390
    Secondary accession number(s): Q84K75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: August 30, 2005
    Last modified: October 1, 2014
    This is version 109 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mutant rescue experiments indicate the existence of a second tryptophan synthase alpha gene whose product might be involved in tryptophan biosynthesis while BX1 might be restricted to free indol production.

    Keywords - Technical termi

    3D-structure

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3