SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P42390

- TRPA_MAIZE

UniProt

P42390 - TRPA_MAIZE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Indole-3-glycerol phosphate lyase, chloroplastic

Gene
BX1
Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. In bacteria, tryptophan synthase alpha (TSA) activity is almost completely dependent on formation of an active alpha2beta2 complex with tryptophan synthase beta (TSB), and indole is usually not released during tryptophan synthesis. In maize, the TSA homolog BX1 catalyzes the formation of free indole from indole-3-glycerol phosphate, independently of TSB.1 Publication

Catalytic activityi

(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate.UniRule annotation
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.UniRule annotation

Kineticsi

  1. KM=0.013 mM for indole-3-glycerol phosphate1 Publication

Pathwayi

GO - Molecular functioni

  1. indole-3-glycerol-phosphate lyase activity Source: UniProtKB-EC
  2. tryptophan synthase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10162.
UniPathwayiUPA00035; UER00044.
UPA00872; UER00847.

Names & Taxonomyi

Protein namesi
Recommended name:
Indole-3-glycerol phosphate lyase, chloroplastic (EC:4.1.2.8)
Alternative name(s):
Benzoxazineless 1
Indole synthase
Tryptophan synthase alpha chain (EC:4.2.1.20)
Gene namesi
Name:BX1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiP42390.
MaizeGDBi102199.

Subcellular locationi

Plastidchloroplast Inferred UniRule annotation

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5353Chloroplast Reviewed predictionAdd
BLAST
Chaini54 – 347294Indole-3-glycerol phosphate lyase, chloroplasticUniRule annotationPRO_0000035782Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains for the tryptophan synthase activity. Homodimer of alpha chains for the indole-3-glycerol phosphate lyase activity.1 Publication

Structurei

Secondary structure

1
347
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi90 – 9910
Beta strandi104 – 1107
Helixi116 – 12813
Beta strandi132 – 1376
Helixi148 – 15811
Turni159 – 1613
Helixi164 – 17411
Helixi175 – 1773
Beta strandi182 – 1854
Helixi189 – 1924
Helixi197 – 2015
Beta strandi206 – 2083
Turni214 – 2163
Helixi217 – 22610
Beta strandi233 – 2353
Helixi241 – 25010
Beta strandi255 – 2584
Helixi273 – 28412
Beta strandi289 – 2935
Helixi298 – 3069
Beta strandi310 – 3145
Helixi316 – 3238
Beta strandi324 – 3274
Helixi328 – 34619

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RD5X-ray2.02A/B87-347[»]
1TJRX-ray2.30A/B87-347[»]
ProteinModelPortaliP42390.
SMRiP42390. Positions 87-347.

Miscellaneous databases

EvolutionaryTraceiP42390.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi47 – 515Poly-ThrUniRule annotation
Compositional biasi52 – 12271Ala-richUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TrpA family.

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000223816.
KOiK13222.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42390-1 [UniParc]FASTAAdd to Basket

« Hide

MAFAPKTSSS SSLSSALQAA QSPPLLLRRM SSTATPRRRY DAAVVVTTTT    50
TARAAAAAVT VPAAPPQAPA PAPVPPKQAA APAERRSRPV SDTMAALMAK 100
GKTAFIPYIT AGDPDLATTA EALRLLDGCG ADVIELGVPC SDPYIDGPII 150
QASVARALAS GTTMDAVLEM LREVTPELSC PVVLLSYYKP IMSRSLAEMK 200
EAGVHGLIVP DLPYVAAHSL WSEAKNNNLE LVLLTTPAIP EDRMKEITKA 250
SEGFVYLVSV NGVTGPRANV NPRVESLIQE VKKVTNKPVA VGFGISKPEH 300
VKQIAQWGAD GVIIGSAMVR QLGEAASPKQ GLRRLEEYAR GMKNALP 347
Length:347
Mass (Da):36,521
Last modified:August 30, 2005 - v2
Checksum:i110D4215101F2012
GO

Sequence cautioni

The sequence CAA54131.1 differs from that shown. Reason: Frameshift at positions 69 and 84.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76713 Genomic DNA. Translation: CAA54131.1. Frameshift.
AY254103 mRNA. Translation: AAP33667.1.
AY254104 mRNA. Translation: AAP33668.1.
PIRiS56665.
RefSeqiNP_001105219.1. NM_001111749.1.
UniGeneiZm.283.

Genome annotation databases

GeneIDi542117.
KEGGizma:542117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X76713 Genomic DNA. Translation: CAA54131.1 . Frameshift.
AY254103 mRNA. Translation: AAP33667.1 .
AY254104 mRNA. Translation: AAP33668.1 .
PIRi S56665.
RefSeqi NP_001105219.1. NM_001111749.1.
UniGenei Zm.283.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RD5 X-ray 2.02 A/B 87-347 [» ]
1TJR X-ray 2.30 A/B 87-347 [» ]
ProteinModelPortali P42390.
SMRi P42390. Positions 87-347.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542117.
KEGGi zma:542117.

Organism-specific databases

Gramenei P42390.
MaizeGDBi 102199.

Phylogenomic databases

HOGENOMi HOG000223816.
KOi K13222.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00044 .
UPA00872 ; UER00847 .
BioCyci MetaCyc:MONOMER-10162.

Miscellaneous databases

EvolutionaryTracei P42390.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00131. Trp_synth_alpha.
InterProi IPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view ]
Pfami PF00290. Trp_syntA. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR00262. trpA. 1 hit.
PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of a maize tryptophan synthase alpha subunit gene with pith enhanced expression."
    Kramer V.C., Koziel M.G.
    Plant Mol. Biol. 27:1183-1188(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. CG000237.
  2. "Transformation of Nicotiana tabacum cv. Samsun with melanin and indigo genes."
    Jordaan A., Botha A.-M.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Crystal structure of tryptophan synthase alpha chain."
    Kulik V., Weyand M., Frey M., Dunn M., Schlichting I.
    Submitted (DEC-2004) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 86-347, SUBUNIT.

Entry informationi

Entry nameiTRPA_MAIZE
AccessioniPrimary (citable) accession number: P42390
Secondary accession number(s): Q84K75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2005
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutant rescue experiments indicate the existence of a second tryptophan synthase alpha gene whose product might be involved in tryptophan biosynthesis while BX1 might be restricted to free indol production.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi