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P42390

- TRPA_MAIZE

UniProt

P42390 - TRPA_MAIZE

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Protein

Indole-3-glycerol phosphate lyase, chloroplastic

Gene

BX1

Organism
Zea mays (Maize)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate. In bacteria, tryptophan synthase alpha (TSA) activity is almost completely dependent on formation of an active alpha2beta2 complex with tryptophan synthase beta (TSB), and indole is usually not released during tryptophan synthesis. In maize, the TSA homolog BX1 catalyzes the formation of free indole from indole-3-glycerol phosphate, independently of TSB.1 Publication

Catalytic activityi

(1S,2R)-1-C-(indol-3-yl)glycerol 3-phosphate = indole + D-glyceraldehyde 3-phosphate.
L-serine + 1-C-(indol-3-yl)glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.

Kineticsi

  1. KM=0.013 mM for indole-3-glycerol phosphate1 Publication

Pathwayi

GO - Molecular functioni

  1. indole-3-glycerol-phosphate lyase activity Source: UniProtKB-EC
  2. tryptophan synthase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Aromatic amino acid biosynthesis, Tryptophan biosynthesis

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-10162.
UniPathwayiUPA00035; UER00044.
UPA00872; UER00847.

Names & Taxonomyi

Protein namesi
Recommended name:
Indole-3-glycerol phosphate lyase, chloroplastic (EC:4.1.2.8)
Alternative name(s):
Benzoxazineless 1
Indole synthase
Tryptophan synthase alpha chain (EC:4.2.1.20)
Gene namesi
Name:BX1
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Organism-specific databases

GrameneiP42390.
MaizeGDBi102199.

Subcellular locationi

Plastidchloroplast Curated

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5353ChloroplastSequence AnalysisAdd
BLAST
Chaini54 – 347294Indole-3-glycerol phosphate lyase, chloroplasticPRO_0000035782Add
BLAST

Interactioni

Subunit structurei

Tetramer of two alpha and two beta chains for the tryptophan synthase activity. Homodimer of alpha chains for the indole-3-glycerol phosphate lyase activity.1 Publication

Structurei

Secondary structure

1
347
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi90 – 9910Combined sources
Beta strandi104 – 1107Combined sources
Helixi116 – 12813Combined sources
Beta strandi132 – 1376Combined sources
Helixi148 – 15811Combined sources
Turni159 – 1613Combined sources
Helixi164 – 17411Combined sources
Helixi175 – 1773Combined sources
Beta strandi182 – 1854Combined sources
Helixi189 – 1924Combined sources
Helixi197 – 2015Combined sources
Beta strandi206 – 2083Combined sources
Turni214 – 2163Combined sources
Helixi217 – 22610Combined sources
Beta strandi233 – 2353Combined sources
Helixi241 – 25010Combined sources
Beta strandi255 – 2584Combined sources
Helixi273 – 28412Combined sources
Beta strandi289 – 2935Combined sources
Helixi298 – 3069Combined sources
Beta strandi310 – 3145Combined sources
Helixi316 – 3238Combined sources
Beta strandi324 – 3274Combined sources
Helixi328 – 34619Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1RD5X-ray2.02A/B87-347[»]
1TJRX-ray2.30A/B87-347[»]
ProteinModelPortaliP42390.
SMRiP42390. Positions 87-347.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42390.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi47 – 515Poly-Thr
Compositional biasi52 – 12271Ala-richAdd
BLAST

Sequence similaritiesi

Belongs to the TrpA family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000223816.
KOiK13222.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00131. Trp_synth_alpha.
InterProiIPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view]
PfamiPF00290. Trp_syntA. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
TIGRFAMsiTIGR00262. trpA. 1 hit.
PROSITEiPS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42390-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAFAPKTSSS SSLSSALQAA QSPPLLLRRM SSTATPRRRY DAAVVVTTTT
60 70 80 90 100
TARAAAAAVT VPAAPPQAPA PAPVPPKQAA APAERRSRPV SDTMAALMAK
110 120 130 140 150
GKTAFIPYIT AGDPDLATTA EALRLLDGCG ADVIELGVPC SDPYIDGPII
160 170 180 190 200
QASVARALAS GTTMDAVLEM LREVTPELSC PVVLLSYYKP IMSRSLAEMK
210 220 230 240 250
EAGVHGLIVP DLPYVAAHSL WSEAKNNNLE LVLLTTPAIP EDRMKEITKA
260 270 280 290 300
SEGFVYLVSV NGVTGPRANV NPRVESLIQE VKKVTNKPVA VGFGISKPEH
310 320 330 340
VKQIAQWGAD GVIIGSAMVR QLGEAASPKQ GLRRLEEYAR GMKNALP
Length:347
Mass (Da):36,521
Last modified:August 30, 2005 - v2
Checksum:i110D4215101F2012
GO

Sequence cautioni

The sequence CAA54131.1 differs from that shown. Reason: Frameshift at positions 69 and 84. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76713 Genomic DNA. Translation: CAA54131.1. Frameshift.
AY254103 mRNA. Translation: AAP33667.1.
AY254104 mRNA. Translation: AAP33668.1.
PIRiS56665.
RefSeqiNP_001105219.1. NM_001111749.1.
UniGeneiZm.283.

Genome annotation databases

GeneIDi542117.
KEGGizma:542117.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X76713 Genomic DNA. Translation: CAA54131.1 . Frameshift.
AY254103 mRNA. Translation: AAP33667.1 .
AY254104 mRNA. Translation: AAP33668.1 .
PIRi S56665.
RefSeqi NP_001105219.1. NM_001111749.1.
UniGenei Zm.283.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1RD5 X-ray 2.02 A/B 87-347 [» ]
1TJR X-ray 2.30 A/B 87-347 [» ]
ProteinModelPortali P42390.
SMRi P42390. Positions 87-347.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 542117.
KEGGi zma:542117.

Organism-specific databases

Gramenei P42390.
MaizeGDBi 102199.

Phylogenomic databases

HOGENOMi HOG000223816.
KOi K13222.

Enzyme and pathway databases

UniPathwayi UPA00035 ; UER00044 .
UPA00872 ; UER00847 .
BioCyci MetaCyc:MONOMER-10162.

Miscellaneous databases

EvolutionaryTracei P42390.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00131. Trp_synth_alpha.
InterProi IPR013785. Aldolase_TIM.
IPR011060. RibuloseP-bd_barrel.
IPR018204. Trp_synthase_alpha_AS.
IPR002028. Trp_synthase_suA.
[Graphical view ]
Pfami PF00290. Trp_syntA. 1 hit.
[Graphical view ]
SUPFAMi SSF51366. SSF51366. 1 hit.
TIGRFAMsi TIGR00262. trpA. 1 hit.
PROSITEi PS00167. TRP_SYNTHASE_ALPHA. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Structure of a maize tryptophan synthase alpha subunit gene with pith enhanced expression."
    Kramer V.C., Koziel M.G.
    Plant Mol. Biol. 27:1183-1188(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: cv. CG000237.
  2. "Transformation of Nicotiana tabacum cv. Samsun with melanin and indigo genes."
    Jordaan A., Botha A.-M.
    Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. Cited for: FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
  4. "Crystal structure of tryptophan synthase alpha chain."
    Kulik V., Weyand M., Frey M., Dunn M., Schlichting I.
    Submitted (DEC-2004) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.02 ANGSTROMS) OF 86-347, SUBUNIT.

Entry informationi

Entry nameiTRPA_MAIZE
AccessioniPrimary (citable) accession number: P42390
Secondary accession number(s): Q84K75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 30, 2005
Last modified: November 26, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

Mutant rescue experiments indicate the existence of a second tryptophan synthase alpha gene whose product might be involved in tryptophan biosynthesis while BX1 might be restricted to free indol production.

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3