P42388 (TRPG_BUCAP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Anthranilate synthase component 2
EC=4.1.3.27

Alternative name(s):
Anthranilate synthase component II
Glutamine amido-transferase

Gene names

Name:	trpG
Ordered Locus Names:	BUsg_PT2

Encoded on

Plasmid pBSc

Organism

Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) [Complete proteome] [HAMAP]

Taxonomic identifier

198804 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Buchnera

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Catalytic activity	Chorismate + L-glutamine = anthranilate + pyruvate + L-glutamate.
Pathway	Amino-acid biosynthesis; L-tryptophan biosynthesis; L-tryptophan from chorismate: step 1/5.
Subunit structure	Tetramer of two components I and two components II.
Miscellaneous	Component I catalyzes the formation of anthranilate using ammonia rather than glutamine, whereas component II provides glutamine amidotransferase activity.
Sequence similarities	Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis Tryptophan biosynthesis
Domain	Glutamine amidotransferase
Molecular function	Lyase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW tryptophan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	anthranilate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 196	196	Anthranilate synthase component 2		PRO_0000056873
Regions
	Domain	3 – 196	194	Glutamine amidotransferase type-1
Sites
	Active site	84	1	By similarity
	Active site	170	1	By similarity
	Active site	172	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42388 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2FB23F9DAB441F3E	FASTA	196	21,867
	10 20 30 40 50 60 MANILLLDNF DSFTYNLVEQ LRNKNSNVLI YRNTVDINTI LNSIKKIRNP ILMLSPGPST 70 80 90 100 110 120 PKNAGCMLNL IKKVKGEIPI VGICLGHQAI VEAYGGIIGY AGEIFHGKAS LINHDGLEMF 130 140 150 160 170 180 EGLPQPLPVA RYHSLICNKI PKNFIINSYF NDMIMSVRNN LDYVCGFQFH PESILTTSGA 190 LLLEKIINWA SLKYKG

References

[1]	"Amplification of trpEG: adaptation of Buchnera aphidicola to an endosymbiotic association with aphids." Lai C.-Y., Baumann L., Baumann P. Proc. Natl. Acad. Sci. U.S.A. 91:3819-3823(1994) [PubMed: 8170994] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Sg.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21938 Genomic DNA. Translation: CAA79932.1.
PIR	I40053.
3D structure databases
ProteinModelPortal	P42388.
SMR	P42388. Positions 2-193.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBBUCT00000000599; EBBUCP00000000555; EBBUCG00000000599.
GenomeReviews	Gene locus BUsg_PT2 in contig Z21938_GR.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000008118.
HOGENOM	HBG292341.
OMA	CAISNTA.
Family and domain databases
InterPro	IPR017926. GATASE_1. IPR006221. TrpG_papA. [Graphical view]
Pfam	PF00117. GATase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00566. TrpG_papA. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRPG_BUCAP

Accession

Primary (citable) accession number: P42388

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program