P42380 (CLPP_CHLRE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp

Gene names

Name:

clpP

Encoded on

Plastid; Chloroplast

Organism

Chlamydomonas reinhardtii (Chlamydomonas smithii) [Reference proteome]

Taxonomic identifier

3055 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	524 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP-Rule MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP-Rule MF_00444
Subunit structure	Component of the chloroplastic Clp protease core complex.
Subcellular location	Plastid › chloroplast stroma By similarity HAMAP-Rule MF_00444.
Domain	This gene contains one large insertion sequence (IS1) that divides the clpP gene into two sequence domains. The insertion sequence forms a continuous open reading frame with its upstream and downstream sequence domains. HAMAP-Rule MF_00444
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 524	524	ATP-dependent Clp protease proteolytic subunit HAMAP-Rule MF_00444		PRO_0000179737
Regions
	Region	60 – 345	286	Insertion IS1 HAMAP-Rule MF_00444
Sites
	Active site	387	1	By similarity
	Active site	412	1	By similarity
Natural variations
	Natural variant	290	1	R → Q in strain: CC-503.

Sequences

	Sequence		Length	Mass (Da)
	P42380 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: ACF82676C1D96813	FASTA	524	59,501
	10 20 30 40 50 60 MPIGVPRIIY CWGEELPAQW TDIYNFIFRR RMVFLMQYLD DELCNQICGL LINIHMEDRS 70 80 90 100 110 120 KELEKKEMEK SGLFKSGTAK TKGKDTVKKE NLSGGASAKR QSVEDLLTSD NDFGIEENHL 130 140 150 160 170 180 LEQYTLQKIT TEWLNWNAQF FDYSDEPYLY YLADILSKDF SPNQDKDSAN LNFAKSSANK 190 200 210 220 230 240 QAFQNPAEMT KLIKNLKNLK NFSTGSKNVK QNLDVYSPFR LLANFAPQNY NLEHPNQNLA 250 260 270 280 290 300 EIYSLLKTST QNTNQPFTKK LIDNLSHKEL MNRLQSPEKL VASSEKALGR RRLKQRYVQE 310 320 330 340 350 360 RLGSGGLSNS KALKAYNYLD QGALNNESGR SLYRKQTERV IQEEESKKVF MIINSFGGSV 370 380 390 400 410 420 GNGITVHDAL QFIKAGSLTL ALGVAASAAS LALAGGTIGE RYVTEGCHTM IHQPEGGLNG 430 440 450 460 470 480 QASDIWIDSQ EIMKIRLDVA EIYSLSTYRP RHKILRDLDR DFYLTAMETI YYGLADEIAT 490 500 510 520 NEVMHSIVEM TNQVWSYHDS KQERLLESRA SLVGDSTQTQ ESNS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth." Huang C., Wang S., Chen L., Lemieux C., Otis C., Turmel M., Liu X.-Q. Mol. Gen. Genet. 244:151-159(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 137c / CC-125.
[2]	"Nucleotide diversity of the Chlamydomonas reinhardtii plastid genome: addressing the mutational-hazard hypothesis." Smith D.R., Lee R.W. BMC Evol. Biol. 9:120-120(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CC-503.
[3]	"The Chlamydomonas reinhardtii plastid chromosome: islands of genes in a sea of repeats." Maul J.E., Lilly J.W., Cui L., dePamphilis C.W., Miller W., Harris E.H., Stern D.B. Plant Cell 14:2659-2679(2002) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION, COMPLETE PLASTID GENOME.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L28803 Genomic DNA. Translation: AAA53086.1. FJ423446 Genomic DNA. Translation: ACJ50097.1. BK000554 Genomic DNA. Translation: DAA00910.1.
PIR	T07988.
RefSeq	NP_958364.1. NC_005353.1.
3D structure databases
ProteinModelPortal	P42380.
ModBase	Search...
Proteomic databases
PRIDE	P42380.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	DAA00910; DAA00910; DAA00910.
GeneID	2717015.
KEGG	cre:ChreCp007.
Phylogenomic databases
ProtClustDB	CHL00028.
Enzyme and pathway databases
BioCyc	CHLAMY:CHRECP007-MONOMER.
BRENDA	3.4.21.92. 1318.
Family and domain databases
HAMAP	MF_00444. ClpP. Divergent sequence.
InterPro	IPR001907. ClpP. IPR023562. ClpP/TepA. IPR018215. ClpP_AS. [Graphical view]
PANTHER	PTHR10381. PTHR10381. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_CHLRE

Accession

Primary (citable) accession number: P42380
Secondary accession number(s): B7U1F0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program