P42379 (CLPP_CHLMO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent Clp protease proteolytic subunit
EC=3.4.21.92

Alternative name(s):
Endopeptidase Clp

Cleaved into the following chain:

1. Ceu clpP intein
   Alternative name(s):
   Insertion IS2

Gene names

Name:

clpP

Encoded on

Plastid; Chloroplast

Organism

Chlamydomonas moewusii (Chlamydomonas eugametos)

Taxonomic identifier

3054 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Chlorophyta › Chlorophyceae › Chlamydomonadales › Chlamydomonadaceae › Chlamydomonas

Protein attributes

Sequence length	1010 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity. HAMAP-Rule MF_00444
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP-Rule MF_00444
Subunit structure	Component of the chloroplastic Clp protease core complex.
Subcellular location	Plastid › chloroplast stroma By similarity.
Domain	This gene contains two large insertion sequences (IS1 and Ceu clpP intein) that divide the clpP gene into three sequence domains. Each insertion sequence forms a continuous open reading frame with its upstream and downstream sequence domains. HAMAP-Rule MF_00444
Post-translational modification	This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation. HAMAP-Rule MF_00444
Sequence similarities	Belongs to the peptidase S14 family. Contains 1 DOD-type homing endonuclease domain.

Ontologies

Keywords
Cellular component	Chloroplast Plastid
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
PTM	Autocatalytic cleavage Protein splicing
Gene Ontology (GO)
Biological_process	intein-mediated protein splicing Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW endonuclease activity Inferred from electronic annotation. Source: InterPro serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 447	447	ATP-dependent Clp protease proteolytic subunit, 1st part HAMAP-Rule MF_00444		PRO_0000005513
	Chain	448 – 903	456	Ceu clpP intein HAMAP-Rule MF_00444		PRO_0000005514
	Chain	904 – 1010	107	ATP-dependent Clp protease proteolytic subunit, 2nd part HAMAP-Rule MF_00444		PRO_0000005515
Regions
	Domain	590 – 728	139	DOD-type homing endonuclease
	Region	60 – 377	318	Insertion IS1 HAMAP-Rule MF_00444
Sites
	Active site	419	1	By similarity
	Active site	444	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42379 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 886463C9F781D0A6	FASTA	1,010	114,552
	10 20 30 40 50 60 MPIGVPRIIY CWGEELPAQW TDIYNFIFRR RMVFLMQYLD DELCNQICGL LINIHMEDRS 70 80 90 100 110 120 KELEKKEIER SGLFKGGPKT QKGGTGAGET GASSIQNKKS NSSSFEDLLA ADEDLGIDEN 130 140 150 160 170 180 NTLEQYTLQK ITMEWLNWNA QFFDYSDEPY LFYLAEMLSK DFNKGDARML FSNNNKFSMP 190 200 210 220 230 240 FSQMLNTGSM SDPRRPQSTN GANWNSSEQN NSLDIYSPFR MLANFEAQDY DFKQINPSLA 250 260 270 280 290 300 SKEEVFKLFN NTILKNGGQR NNNMSKLLTE LAQRNWENKT NSQENLYKST EKALSQRNLR 310 320 330 340 350 360 KEYIKDRTLN NYSSDPFNTK GYVNAQGAST GPSPRTRGMH ADGSLNYLDF YSYNDSYNDF 370 380 390 400 410 420 KTAPRGKQAE RAFQEEESKK VFVIINSFGG SVGNGITVHD ALQFIKAGSL TLALGVAASA 430 440 450 460 470 480 ASLALAGGTI GERYVTEGCH VMIHQPECLT SDHTVLTTRG WIPIADVTLD DKVAVLDNNT 490 500 510 520 530 540 GEMSYQNPQK VHKYDYEGPM YEVKTAGVDL FVTPNHRMYV NTTNNTTNQN YNLVEASSIF 550 560 570 580 590 600 GKKVRYKNDA IWNKTDYQFI LPETATLTGH TNKISSTPAI QPEMNAWLTF FGLWIANGHT 610 620 630 640 650 660 TKIAEKTAEN NQQKQRYKVI LTQVKEDVCD IIEQTLNKLG FNFIRSGKDY TIENKQLWSY 670 680 690 700 710 720 LNPFDNGALN KYLPDWVWEL SSQQCKILLN SLCLGNCLFT KNDDTLHYFS TSERFANDVS 730 740 750 760 770 780 RLALHAGTTS TIQLEAAPSN LYDTIIGLPV EVNTTLWRVI INQSSFYSYS TDKSSALNLS 790 800 810 820 830 840 NNVACYVNAQ SALTLEQNSQ KINKNTLVLT KNNVKSQTMH SQRAERVDTA LLTQKELDNS 850 860 870 880 890 900 LNHEILINKN PGTSQLECVV NPEVNNTSTN DRFVYYKGPV YCLTGPNNVF YVQRNGKAVW 910 920 930 940 950 960 TGNSSIQGQA SDIWIDSQEI MKIRLDVAEI YSLATYRPRH KILRDLDRDF YLTATETIHY 970 980 990 1000 1010 GLADEIASNE VMQEIIEMTS KVWDYHDTKQ QRLLESRDST TSGADTQSQN

References

[1]	"The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth." Huang C., Wang S., Chen L., Lemieux C., Otis C., Turmel M., Liu X.-Q. Mol. Gen. Genet. 244:151-159(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Identification of an unusual intein in chloroplast ClpP protease of Chlamydomonas eugametos." Wang S., Liu X.-Q. J. Biol. Chem. 272:11869-11873(1997) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SPLICING.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L29402 Genomic DNA. Translation: AAA84150.1.
PIR	T09499.
3D structure databases
ProteinModelPortal	P42379.
ModBase	Search...
Protein family/group databases
MEROPS	S14.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.4.21.92. 1316.
Family and domain databases
HAMAP	MF_00444. ClpP. Divergent sequence.
InterPro	IPR001907. ClpP. IPR023562. ClpP/TepA. IPR018215. ClpP_AS. IPR003587. Hint_dom_N. IPR004042. Intein_endonuc. IPR006141. Intein_splice_site. [Graphical view]
PANTHER	PTHR10381. PTHR10381. 1 hit.
Pfam	PF00574. CLP_protease. 2 hits. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
SMART	SM00306. HintN. 1 hit. [Graphical view]
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. 1 hit. PS50818. INTEIN_C_TER. False negative. PS50819. INTEIN_ENDONUCLEASE. 1 hit. PS50817. INTEIN_N_TER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP_CHLMO

Accession

Primary (citable) accession number: P42379

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program