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P42379

- CLPP_CHLMO

UniProt

P42379 - CLPP_CHLMO

Protein

ATP-dependent Clp protease proteolytic subunit

Gene

clpP

Organism
Chlamydomonas moewusii (Chlamydomonas eugametos)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity.By similarity

    Catalytic activityi

    Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei419 – 4191NucleophilePROSITE-ProRule annotation
    Active sitei444 – 4441By similarity

    GO - Molecular functioni

    1. endonuclease activity Source: InterPro
    2. serine-type endopeptidase activity Source: InterPro

    GO - Biological processi

    1. intein-mediated protein splicing Source: InterPro

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Enzyme and pathway databases

    BRENDAi3.4.21.92. 1316.

    Protein family/group databases

    MEROPSiN11.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent Clp protease proteolytic subunit (EC:3.4.21.92)
    Alternative name(s):
    Endopeptidase Clp
    Cleaved into the following chain:
    Alternative name(s):
    Insertion IS2
    Gene namesi
    Name:clpP
    Encoded oniPlastid; Chloroplast
    OrganismiChlamydomonas moewusii (Chlamydomonas eugametos)
    Taxonomic identifieri3054 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

    Subcellular locationi

    Plastidchloroplast stroma By similarity

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 447447ATP-dependent Clp protease proteolytic subunit, 1st partPRO_0000005513Add
    BLAST
    Chaini448 – 903456Ceu clpP inteinPRO_0000005514Add
    BLAST
    Chaini904 – 1010107ATP-dependent Clp protease proteolytic subunit, 2nd partPRO_0000005515Add
    BLAST

    Post-translational modificationi

    This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

    Keywords - PTMi

    Autocatalytic cleavage, Protein splicing

    Interactioni

    Subunit structurei

    Component of the chloroplastic Clp protease core complex.

    Structurei

    3D structure databases

    ProteinModelPortaliP42379.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini590 – 728139DOD-type homing endonucleasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni60 – 377318Insertion IS1Add
    BLAST

    Domaini

    This gene contains two large insertion sequences (IS1 and Ceu clpP intein) that divide the clpP gene into three sequence domains. Each insertion sequence forms a continuous open reading frame with its upstream and downstream sequence domains.

    Sequence similaritiesi

    Belongs to the peptidase S14 family.Curated
    Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di2.170.16.10. 1 hit.
    3.10.28.10. 1 hit.
    3.90.226.10. 2 hits.
    InterProiIPR001907. ClpP.
    IPR029045. ClpP/crotonase-like_dom.
    IPR023562. ClpP/TepA.
    IPR018215. ClpP_AS.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    [Graphical view]
    PANTHERiPTHR10381. PTHR10381. 1 hit.
    PfamiPF00574. CLP_protease. 2 hits.
    [Graphical view]
    PRINTSiPR00127. CLPPROTEASEP.
    SMARTiSM00306. HintN. 1 hit.
    [Graphical view]
    SUPFAMiSSF51294. SSF51294. 2 hits.
    SSF52096. SSF52096. 2 hits.
    PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
    PS00381. CLP_PROTEASE_SER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42379-1 [UniParc]FASTAAdd to Basket

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    MPIGVPRIIY CWGEELPAQW TDIYNFIFRR RMVFLMQYLD DELCNQICGL     50
    LINIHMEDRS KELEKKEIER SGLFKGGPKT QKGGTGAGET GASSIQNKKS 100
    NSSSFEDLLA ADEDLGIDEN NTLEQYTLQK ITMEWLNWNA QFFDYSDEPY 150
    LFYLAEMLSK DFNKGDARML FSNNNKFSMP FSQMLNTGSM SDPRRPQSTN 200
    GANWNSSEQN NSLDIYSPFR MLANFEAQDY DFKQINPSLA SKEEVFKLFN 250
    NTILKNGGQR NNNMSKLLTE LAQRNWENKT NSQENLYKST EKALSQRNLR 300
    KEYIKDRTLN NYSSDPFNTK GYVNAQGAST GPSPRTRGMH ADGSLNYLDF 350
    YSYNDSYNDF KTAPRGKQAE RAFQEEESKK VFVIINSFGG SVGNGITVHD 400
    ALQFIKAGSL TLALGVAASA ASLALAGGTI GERYVTEGCH VMIHQPECLT 450
    SDHTVLTTRG WIPIADVTLD DKVAVLDNNT GEMSYQNPQK VHKYDYEGPM 500
    YEVKTAGVDL FVTPNHRMYV NTTNNTTNQN YNLVEASSIF GKKVRYKNDA 550
    IWNKTDYQFI LPETATLTGH TNKISSTPAI QPEMNAWLTF FGLWIANGHT 600
    TKIAEKTAEN NQQKQRYKVI LTQVKEDVCD IIEQTLNKLG FNFIRSGKDY 650
    TIENKQLWSY LNPFDNGALN KYLPDWVWEL SSQQCKILLN SLCLGNCLFT 700
    KNDDTLHYFS TSERFANDVS RLALHAGTTS TIQLEAAPSN LYDTIIGLPV 750
    EVNTTLWRVI INQSSFYSYS TDKSSALNLS NNVACYVNAQ SALTLEQNSQ 800
    KINKNTLVLT KNNVKSQTMH SQRAERVDTA LLTQKELDNS LNHEILINKN 850
    PGTSQLECVV NPEVNNTSTN DRFVYYKGPV YCLTGPNNVF YVQRNGKAVW 900
    TGNSSIQGQA SDIWIDSQEI MKIRLDVAEI YSLATYRPRH KILRDLDRDF 950
    YLTATETIHY GLADEIASNE VMQEIIEMTS KVWDYHDTKQ QRLLESRDST 1000
    TSGADTQSQN 1010
    Length:1,010
    Mass (Da):114,552
    Last modified:November 1, 1995 - v1
    Checksum:i886463C9F781D0A6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29402 Genomic DNA. Translation: AAA84150.1.
    PIRiT09499.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L29402 Genomic DNA. Translation: AAA84150.1 .
    PIRi T09499.

    3D structure databases

    ProteinModelPortali P42379.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi N11.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    BRENDAi 3.4.21.92. 1316.

    Family and domain databases

    Gene3Di 2.170.16.10. 1 hit.
    3.10.28.10. 1 hit.
    3.90.226.10. 2 hits.
    InterProi IPR001907. ClpP.
    IPR029045. ClpP/crotonase-like_dom.
    IPR023562. ClpP/TepA.
    IPR018215. ClpP_AS.
    IPR028992. Hedgehog/Intein_dom.
    IPR003587. Hint_dom_N.
    IPR027434. Homing_endonucl.
    IPR004042. Intein_endonuc.
    IPR006141. Intein_splice_site.
    [Graphical view ]
    PANTHERi PTHR10381. PTHR10381. 1 hit.
    Pfami PF00574. CLP_protease. 2 hits.
    [Graphical view ]
    PRINTSi PR00127. CLPPROTEASEP.
    SMARTi SM00306. HintN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51294. SSF51294. 2 hits.
    SSF52096. SSF52096. 2 hits.
    PROSITEi PS00382. CLP_PROTEASE_HIS. 1 hit.
    PS00381. CLP_PROTEASE_SER. 1 hit.
    PS50819. INTEIN_ENDONUCLEASE. 1 hit.
    PS50817. INTEIN_N_TER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth."
      Huang C., Wang S., Chen L., Lemieux C., Otis C., Turmel M., Liu X.-Q.
      Mol. Gen. Genet. 244:151-159(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Identification of an unusual intein in chloroplast ClpP protease of Chlamydomonas eugametos."
      Wang S., Liu X.-Q.
      J. Biol. Chem. 272:11869-11873(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SPLICING.

    Entry informationi

    Entry nameiCLPP_CHLMO
    AccessioniPrimary (citable) accession number: P42379
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Intein-containing proteins
      List of intein-containing protein entries
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3