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P42379

- CLPP_CHLMO

UniProt

P42379 - CLPP_CHLMO

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Protein
ATP-dependent Clp protease proteolytic subunit
Gene
clpP
Organism
Chlamydomonas moewusii (Chlamydomonas eugametos)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity.

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei419 – 4191Nucleophile By similarity
Active sitei444 – 4441 By similarity

GO - Molecular functioni

  1. endonuclease activity Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.92. 1316.

Protein family/group databases

MEROPSiN11.001.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit (EC:3.4.21.92)
Alternative name(s):
Endopeptidase Clp
Cleaved into the following chain:
Alternative name(s):
Insertion IS2
Gene namesi
Name:clpP
Encoded oniPlastid; Chloroplast
OrganismiChlamydomonas moewusii (Chlamydomonas eugametos)
Taxonomic identifieri3054 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

Plastidchloroplast stroma By similarity

GO - Cellular componenti

  1. chloroplast stroma Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447ATP-dependent Clp protease proteolytic subunit, 1st part
PRO_0000005513Add
BLAST
Chaini448 – 903456Ceu clpP intein
PRO_0000005514Add
BLAST
Chaini904 – 1010107ATP-dependent Clp protease proteolytic subunit, 2nd part
PRO_0000005515Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Component of the chloroplastic Clp protease core complex.

Structurei

3D structure databases

ProteinModelPortaliP42379.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini590 – 728139DOD-type homing endonuclease
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 377318Insertion IS1
Add
BLAST

Domaini

This gene contains two large insertion sequences (IS1 and Ceu clpP intein) that divide the clpP gene into three sequence domains. Each insertion sequence forms a continuous open reading frame with its upstream and downstream sequence domains.

Sequence similaritiesi

Belongs to the peptidase S14 family.

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.10.28.10. 1 hit.
3.90.226.10. 2 hits.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR018215. ClpP_AS.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 2 hits.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SMARTiSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52096. SSF52096. 2 hits.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42379-1 [UniParc]FASTAAdd to Basket

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MPIGVPRIIY CWGEELPAQW TDIYNFIFRR RMVFLMQYLD DELCNQICGL     50
LINIHMEDRS KELEKKEIER SGLFKGGPKT QKGGTGAGET GASSIQNKKS 100
NSSSFEDLLA ADEDLGIDEN NTLEQYTLQK ITMEWLNWNA QFFDYSDEPY 150
LFYLAEMLSK DFNKGDARML FSNNNKFSMP FSQMLNTGSM SDPRRPQSTN 200
GANWNSSEQN NSLDIYSPFR MLANFEAQDY DFKQINPSLA SKEEVFKLFN 250
NTILKNGGQR NNNMSKLLTE LAQRNWENKT NSQENLYKST EKALSQRNLR 300
KEYIKDRTLN NYSSDPFNTK GYVNAQGAST GPSPRTRGMH ADGSLNYLDF 350
YSYNDSYNDF KTAPRGKQAE RAFQEEESKK VFVIINSFGG SVGNGITVHD 400
ALQFIKAGSL TLALGVAASA ASLALAGGTI GERYVTEGCH VMIHQPECLT 450
SDHTVLTTRG WIPIADVTLD DKVAVLDNNT GEMSYQNPQK VHKYDYEGPM 500
YEVKTAGVDL FVTPNHRMYV NTTNNTTNQN YNLVEASSIF GKKVRYKNDA 550
IWNKTDYQFI LPETATLTGH TNKISSTPAI QPEMNAWLTF FGLWIANGHT 600
TKIAEKTAEN NQQKQRYKVI LTQVKEDVCD IIEQTLNKLG FNFIRSGKDY 650
TIENKQLWSY LNPFDNGALN KYLPDWVWEL SSQQCKILLN SLCLGNCLFT 700
KNDDTLHYFS TSERFANDVS RLALHAGTTS TIQLEAAPSN LYDTIIGLPV 750
EVNTTLWRVI INQSSFYSYS TDKSSALNLS NNVACYVNAQ SALTLEQNSQ 800
KINKNTLVLT KNNVKSQTMH SQRAERVDTA LLTQKELDNS LNHEILINKN 850
PGTSQLECVV NPEVNNTSTN DRFVYYKGPV YCLTGPNNVF YVQRNGKAVW 900
TGNSSIQGQA SDIWIDSQEI MKIRLDVAEI YSLATYRPRH KILRDLDRDF 950
YLTATETIHY GLADEIASNE VMQEIIEMTS KVWDYHDTKQ QRLLESRDST 1000
TSGADTQSQN 1010
Length:1,010
Mass (Da):114,552
Last modified:November 1, 1995 - v1
Checksum:i886463C9F781D0A6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L29402 Genomic DNA. Translation: AAA84150.1.
PIRiT09499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L29402 Genomic DNA. Translation: AAA84150.1 .
PIRi T09499.

3D structure databases

ProteinModelPortali P42379.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi N11.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 3.4.21.92. 1316.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
3.10.28.10. 1 hit.
3.90.226.10. 2 hits.
InterProi IPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR018215. ClpP_AS.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
[Graphical view ]
PANTHERi PTHR10381. PTHR10381. 1 hit.
Pfami PF00574. CLP_protease. 2 hits.
[Graphical view ]
PRINTSi PR00127. CLPPROTEASEP.
SMARTi SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF52096. SSF52096. 2 hits.
PROSITEi PS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth."
    Huang C., Wang S., Chen L., Lemieux C., Otis C., Turmel M., Liu X.-Q.
    Mol. Gen. Genet. 244:151-159(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of an unusual intein in chloroplast ClpP protease of Chlamydomonas eugametos."
    Wang S., Liu X.-Q.
    J. Biol. Chem. 272:11869-11873(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SPLICING.

Entry informationi

Entry nameiCLPP_CHLMO
AccessioniPrimary (citable) accession number: P42379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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