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P42379

- CLPP_CHLMO

UniProt

P42379 - CLPP_CHLMO

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Protein

ATP-dependent Clp protease proteolytic subunit

Gene

clpP

Organism
Chlamydomonas moewusii (Chlamydomonas eugametos)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins (By similarity).By similarity

Catalytic activityi

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei419 – 4191NucleophilePROSITE-ProRule annotation
Active sitei444 – 4441By similarity

GO - Molecular functioni

  1. endonuclease activity Source: InterPro
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. intein-mediated protein splicing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Enzyme and pathway databases

BRENDAi3.4.21.92. 1316.

Protein family/group databases

MEROPSiN11.001.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent Clp protease proteolytic subunit (EC:3.4.21.92)
Alternative name(s):
Endopeptidase Clp
Cleaved into the following chain:
Alternative name(s):
Insertion IS2
Gene namesi
Name:clpP
Encoded oniPlastid; Chloroplast
OrganismiChlamydomonas moewusii (Chlamydomonas eugametos)
Taxonomic identifieri3054 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Subcellular locationi

Plastidchloroplast stroma By similarity

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 447447ATP-dependent Clp protease proteolytic subunit, 1st partPRO_0000005513Add
BLAST
Chaini448 – 903456Ceu clpP inteinPRO_0000005514Add
BLAST
Chaini904 – 1010107ATP-dependent Clp protease proteolytic subunit, 2nd partPRO_0000005515Add
BLAST

Post-translational modificationi

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Keywords - PTMi

Autocatalytic cleavage, Protein splicing

Interactioni

Subunit structurei

Component of the chloroplastic Clp protease core complex.

Structurei

3D structure databases

ProteinModelPortaliP42379.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini590 – 728139DOD-type homing endonucleasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni60 – 377318Insertion IS1Add
BLAST

Domaini

This gene contains two large insertion sequences (IS1 and Ceu clpP intein) that divide the clpP gene into three sequence domains. Each insertion sequence forms a continuous open reading frame with its upstream and downstream sequence domains.

Sequence similaritiesi

Belongs to the peptidase S14 family.Curated
Contains 1 DOD-type homing endonuclease domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.170.16.10. 1 hit.
3.10.28.10. 1 hit.
3.90.226.10. 2 hits.
InterProiIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR018215. ClpP_AS.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
[Graphical view]
PANTHERiPTHR10381. PTHR10381. 1 hit.
PfamiPF00574. CLP_protease. 2 hits.
[Graphical view]
PRINTSiPR00127. CLPPROTEASEP.
SMARTiSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMiSSF51294. SSF51294. 2 hits.
SSF52096. SSF52096. 2 hits.
PROSITEiPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42379-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPIGVPRIIY CWGEELPAQW TDIYNFIFRR RMVFLMQYLD DELCNQICGL
60 70 80 90 100
LINIHMEDRS KELEKKEIER SGLFKGGPKT QKGGTGAGET GASSIQNKKS
110 120 130 140 150
NSSSFEDLLA ADEDLGIDEN NTLEQYTLQK ITMEWLNWNA QFFDYSDEPY
160 170 180 190 200
LFYLAEMLSK DFNKGDARML FSNNNKFSMP FSQMLNTGSM SDPRRPQSTN
210 220 230 240 250
GANWNSSEQN NSLDIYSPFR MLANFEAQDY DFKQINPSLA SKEEVFKLFN
260 270 280 290 300
NTILKNGGQR NNNMSKLLTE LAQRNWENKT NSQENLYKST EKALSQRNLR
310 320 330 340 350
KEYIKDRTLN NYSSDPFNTK GYVNAQGAST GPSPRTRGMH ADGSLNYLDF
360 370 380 390 400
YSYNDSYNDF KTAPRGKQAE RAFQEEESKK VFVIINSFGG SVGNGITVHD
410 420 430 440 450
ALQFIKAGSL TLALGVAASA ASLALAGGTI GERYVTEGCH VMIHQPECLT
460 470 480 490 500
SDHTVLTTRG WIPIADVTLD DKVAVLDNNT GEMSYQNPQK VHKYDYEGPM
510 520 530 540 550
YEVKTAGVDL FVTPNHRMYV NTTNNTTNQN YNLVEASSIF GKKVRYKNDA
560 570 580 590 600
IWNKTDYQFI LPETATLTGH TNKISSTPAI QPEMNAWLTF FGLWIANGHT
610 620 630 640 650
TKIAEKTAEN NQQKQRYKVI LTQVKEDVCD IIEQTLNKLG FNFIRSGKDY
660 670 680 690 700
TIENKQLWSY LNPFDNGALN KYLPDWVWEL SSQQCKILLN SLCLGNCLFT
710 720 730 740 750
KNDDTLHYFS TSERFANDVS RLALHAGTTS TIQLEAAPSN LYDTIIGLPV
760 770 780 790 800
EVNTTLWRVI INQSSFYSYS TDKSSALNLS NNVACYVNAQ SALTLEQNSQ
810 820 830 840 850
KINKNTLVLT KNNVKSQTMH SQRAERVDTA LLTQKELDNS LNHEILINKN
860 870 880 890 900
PGTSQLECVV NPEVNNTSTN DRFVYYKGPV YCLTGPNNVF YVQRNGKAVW
910 920 930 940 950
TGNSSIQGQA SDIWIDSQEI MKIRLDVAEI YSLATYRPRH KILRDLDRDF
960 970 980 990 1000
YLTATETIHY GLADEIASNE VMQEIIEMTS KVWDYHDTKQ QRLLESRDST
1010
TSGADTQSQN
Length:1,010
Mass (Da):114,552
Last modified:November 1, 1995 - v1
Checksum:i886463C9F781D0A6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29402 Genomic DNA. Translation: AAA84150.1.
PIRiT09499.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29402 Genomic DNA. Translation: AAA84150.1 .
PIRi T09499.

3D structure databases

ProteinModelPortali P42379.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi N11.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

BRENDAi 3.4.21.92. 1316.

Family and domain databases

Gene3Di 2.170.16.10. 1 hit.
3.10.28.10. 1 hit.
3.90.226.10. 2 hits.
InterProi IPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR018215. ClpP_AS.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
[Graphical view ]
PANTHERi PTHR10381. PTHR10381. 1 hit.
Pfami PF00574. CLP_protease. 2 hits.
[Graphical view ]
PRINTSi PR00127. CLPPROTEASEP.
SMARTi SM00306. HintN. 1 hit.
[Graphical view ]
SUPFAMi SSF51294. SSF51294. 2 hits.
SSF52096. SSF52096. 2 hits.
PROSITEi PS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth."
    Huang C., Wang S., Chen L., Lemieux C., Otis C., Turmel M., Liu X.-Q.
    Mol. Gen. Genet. 244:151-159(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Identification of an unusual intein in chloroplast ClpP protease of Chlamydomonas eugametos."
    Wang S., Liu X.-Q.
    J. Biol. Chem. 272:11869-11873(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SPLICING.

Entry informationi

Entry nameiCLPP_CHLMO
AccessioniPrimary (citable) accession number: P42379
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Intein-containing proteins
    List of intein-containing protein entries
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3