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P42379 (CLPP_CHLMO) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent Clp protease proteolytic subunit

EC=3.4.21.92
Alternative name(s):
Endopeptidase Clp

Cleaved into the following chain:

  1. Ceu clpP intein
    Alternative name(s):
    Insertion IS2
Gene names
Name:clpP
Encoded onPlastid; Chloroplast
OrganismChlamydomonas moewusii (Chlamydomonas eugametos)
Taxonomic identifier3054 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeChlorophytaChlorophyceaeChlamydomonadalesChlamydomonadaceaeChlamydomonas

Protein attributes

Sequence length1010 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity.

Catalytic activity

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Subunit structure

Component of the chloroplastic Clp protease core complex.

Subcellular location

Plastidchloroplast stroma By similarity.

Domain

This gene contains two large insertion sequences (IS1 and Ceu clpP intein) that divide the clpP gene into three sequence domains. Each insertion sequence forms a continuous open reading frame with its upstream and downstream sequence domains.

Post-translational modification

This protein undergoes a protein self splicing that involves a post-translational excision of the intervening region (intein) followed by peptide ligation.

Sequence similarities

Belongs to the peptidase S14 family.

Contains 1 DOD-type homing endonuclease domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 447447ATP-dependent Clp protease proteolytic subunit, 1st part
PRO_0000005513
Chain448 – 903456Ceu clpP intein
PRO_0000005514
Chain904 – 1010107ATP-dependent Clp protease proteolytic subunit, 2nd part
PRO_0000005515

Regions

Domain590 – 728139DOD-type homing endonuclease
Region60 – 377318Insertion IS1

Sites

Active site4191Nucleophile By similarity
Active site4441 By similarity

Sequences

Sequence LengthMass (Da)Tools
P42379 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 886463C9F781D0A6

FASTA1,010114,552
        10         20         30         40         50         60 
MPIGVPRIIY CWGEELPAQW TDIYNFIFRR RMVFLMQYLD DELCNQICGL LINIHMEDRS 

        70         80         90        100        110        120 
KELEKKEIER SGLFKGGPKT QKGGTGAGET GASSIQNKKS NSSSFEDLLA ADEDLGIDEN 

       130        140        150        160        170        180 
NTLEQYTLQK ITMEWLNWNA QFFDYSDEPY LFYLAEMLSK DFNKGDARML FSNNNKFSMP 

       190        200        210        220        230        240 
FSQMLNTGSM SDPRRPQSTN GANWNSSEQN NSLDIYSPFR MLANFEAQDY DFKQINPSLA 

       250        260        270        280        290        300 
SKEEVFKLFN NTILKNGGQR NNNMSKLLTE LAQRNWENKT NSQENLYKST EKALSQRNLR 

       310        320        330        340        350        360 
KEYIKDRTLN NYSSDPFNTK GYVNAQGAST GPSPRTRGMH ADGSLNYLDF YSYNDSYNDF 

       370        380        390        400        410        420 
KTAPRGKQAE RAFQEEESKK VFVIINSFGG SVGNGITVHD ALQFIKAGSL TLALGVAASA 

       430        440        450        460        470        480 
ASLALAGGTI GERYVTEGCH VMIHQPECLT SDHTVLTTRG WIPIADVTLD DKVAVLDNNT 

       490        500        510        520        530        540 
GEMSYQNPQK VHKYDYEGPM YEVKTAGVDL FVTPNHRMYV NTTNNTTNQN YNLVEASSIF 

       550        560        570        580        590        600 
GKKVRYKNDA IWNKTDYQFI LPETATLTGH TNKISSTPAI QPEMNAWLTF FGLWIANGHT 

       610        620        630        640        650        660 
TKIAEKTAEN NQQKQRYKVI LTQVKEDVCD IIEQTLNKLG FNFIRSGKDY TIENKQLWSY 

       670        680        690        700        710        720 
LNPFDNGALN KYLPDWVWEL SSQQCKILLN SLCLGNCLFT KNDDTLHYFS TSERFANDVS 

       730        740        750        760        770        780 
RLALHAGTTS TIQLEAAPSN LYDTIIGLPV EVNTTLWRVI INQSSFYSYS TDKSSALNLS 

       790        800        810        820        830        840 
NNVACYVNAQ SALTLEQNSQ KINKNTLVLT KNNVKSQTMH SQRAERVDTA LLTQKELDNS 

       850        860        870        880        890        900 
LNHEILINKN PGTSQLECVV NPEVNNTSTN DRFVYYKGPV YCLTGPNNVF YVQRNGKAVW 

       910        920        930        940        950        960 
TGNSSIQGQA SDIWIDSQEI MKIRLDVAEI YSLATYRPRH KILRDLDRDF YLTATETIHY 

       970        980        990       1000       1010 
GLADEIASNE VMQEIIEMTS KVWDYHDTKQ QRLLESRDST TSGADTQSQN 

« Hide

References

[1]"The Chlamydomonas chloroplast clpP gene contains translated large insertion sequences and is essential for cell growth."
Huang C., Wang S., Chen L., Lemieux C., Otis C., Turmel M., Liu X.-Q.
Mol. Gen. Genet. 244:151-159(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Identification of an unusual intein in chloroplast ClpP protease of Chlamydomonas eugametos."
Wang S., Liu X.-Q.
J. Biol. Chem. 272:11869-11873(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SPLICING.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L29402 Genomic DNA. Translation: AAA84150.1.
PIRT09499.

3D structure databases

ProteinModelPortalP42379.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSN11.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

BRENDA3.4.21.92. 1316.

Family and domain databases

Gene3D2.170.16.10. 1 hit.
3.10.28.10. 1 hit.
3.90.226.10. 2 hits.
InterProIPR001907. ClpP.
IPR029045. ClpP/crotonase-like_dom.
IPR023562. ClpP/TepA.
IPR018215. ClpP_AS.
IPR028992. Hedgehog/Intein_dom.
IPR003587. Hint_dom_N.
IPR027434. Homing_endonucl.
IPR004042. Intein_endonuc.
IPR006141. Intein_splice_site.
[Graphical view]
PANTHERPTHR10381. PTHR10381. 1 hit.
PfamPF00574. CLP_protease. 2 hits.
[Graphical view]
PRINTSPR00127. CLPPROTEASEP.
SMARTSM00306. HintN. 1 hit.
[Graphical view]
SUPFAMSSF51294. SSF51294. 2 hits.
SSF52096. SSF52096. 2 hits.
PROSITEPS00382. CLP_PROTEASE_HIS. 1 hit.
PS00381. CLP_PROTEASE_SER. 1 hit.
PS50819. INTEIN_ENDONUCLEASE. 1 hit.
PS50817. INTEIN_N_TER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCLPP_CHLMO
AccessionPrimary (citable) accession number: P42379
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

Intein-containing proteins

List of intein-containing protein entries