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Protein

Chaperone protein DnaK

Gene

dnaK

Organism
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Acts as a chaperone.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Chaperone

Keywords - Biological processi

Stress response

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-188-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaK
Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70
Gene namesi
Name:dnaK
Ordered Locus Names:R00182
ORF Names:SMc02857
OrganismiRhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti)
Taxonomic identifieri266834 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesRhizobiaceaeSinorhizobium/Ensifer groupSinorhizobium
Proteomesi
  • UP000001976 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 641641Chaperone protein DnaKPRO_0000078525Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei198 – 1981Phosphothreonine; by autocatalysisBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiP42374.

Expressioni

Inductioni

By stress conditions e.g. heat shock (By similarity).By similarity

Interactioni

Protein-protein interaction databases

STRINGi266834.SMc02857.

Structurei

3D structure databases

ProteinModelPortaliP42374.
SMRiP42374. Positions 1-595.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the heat shock protein 70 family.Curated

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228136.
KOiK04043.
OMAiEKMAPPQ.
OrthoDBiEOG6JMMSV.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42374-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKVIGIDLG TTNSCVSVMD GKDAKVIENA EGARTTPSMV AFTEDGERLV
60 70 80 90 100
GQPAKRQAVT NPENTLFAIK RLIGRTFEDP TTQKDKGMVP YKIVKADNGD
110 120 130 140 150
AWVEAHGTSY SPSQISAMIL QKMKETAESY LGEKVEKAVI TVPAYFNDAQ
160 170 180 190 200
RQATKDAGKI AGLDVLRIIN EPTAAALAYG LDKKEGKTIA VYDLGGGTFD
210 220 230 240 250
ISVLEIGDGV FEVKSTNGDT FLGGEDFDMR LVEYLASEFK KEQGIDLKND
260 270 280 290 300
KLALQRLKEA AEKAKIELSS SQQTEINLPF ITADASGPKH LTMKLSRAKF
310 320 330 340 350
ESLVEDLIQK TIAPCKAALK DAGVSAAEID EVVLVGGMTR MPKVQETVKQ
360 370 380 390 400
LFGKEPHKGV NPDEVVAMGA AIQAGVLQGD VKDVLLLDVT PLSLGIETLG
410 420 430 440 450
GVFTRLIERN TTIPTKKSQV FSTADDNQSA VTIRVSQGER EMAADNKLLG
460 470 480 490 500
QFDLVGIPPA PRGVPQIEVT FDIDANGIVQ VSAKDKGTGK EHQIRIQASG
510 520 530 540 550
GLSDAEIEKM VKDAEANAEA DKKRREGVEA KNQAESLVHS SEKSLQEHGD
560 570 580 590 600
KVSETDRKAI EDAIAALKSA VEVSEPDAED IKAKTNTLME VSMKLGQAIY
610 620 630 640
EAQQTDAAHA DAAADAKRSG DDVVDADYEE VKDEDDRKRS A
Length:641
Mass (Da):68,950
Last modified:November 2, 2001 - v2
Checksum:iB711D9BD9CC2360C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti405 – 4051R → V in AAA64925 (PubMed:7528198).Curated
Sequence conflicti570 – 5701A → R in AAA64925 (PubMed:7528198).Curated
Sequence conflicti611 – 6144DAAA → MPPP in AAA64925 (PubMed:7528198).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36602 Genomic DNA. Translation: AAA64925.1.
AL591688 Genomic DNA. Translation: CAC41569.1.
AF074451 Genomic DNA. Translation: AAD42995.1.
RefSeqiNP_384288.1. NC_003047.1.
WP_003531910.1. NC_003047.1.

Genome annotation databases

EnsemblBacteriaiCAC41569; CAC41569; SMc02857.
GeneIDi1231813.
KEGGisme:SMc02857.
PATRICi23629543. VBISinMel96828_1544.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36602 Genomic DNA. Translation: AAA64925.1.
AL591688 Genomic DNA. Translation: CAC41569.1.
AF074451 Genomic DNA. Translation: AAD42995.1.
RefSeqiNP_384288.1. NC_003047.1.
WP_003531910.1. NC_003047.1.

3D structure databases

ProteinModelPortaliP42374.
SMRiP42374. Positions 1-595.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi266834.SMc02857.

Proteomic databases

PRIDEiP42374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC41569; CAC41569; SMc02857.
GeneIDi1231813.
KEGGisme:SMc02857.
PATRICi23629543. VBISinMel96828_1544.

Phylogenomic databases

eggNOGiENOG4105CFG. Bacteria.
COG0443. LUCA.
HOGENOMiHOG000228136.
KOiK04043.
OMAiEKMAPPQ.
OrthoDBiEOG6JMMSV.

Enzyme and pathway databases

BioCyciSMEL266834:GJF6-188-MONOMER.

Family and domain databases

Gene3Di1.20.1270.10. 1 hit.
2.60.34.10. 1 hit.
HAMAPiMF_00332. DnaK.
InterProiIPR012725. Chaperone_DnaK.
IPR018181. Heat_shock_70_CS.
IPR029048. HSP70_C.
IPR029047. HSP70_peptide-bd.
IPR013126. Hsp_70_fam.
[Graphical view]
PfamiPF00012. HSP70. 1 hit.
[Graphical view]
PRINTSiPR00301. HEATSHOCK70.
SUPFAMiSSF100920. SSF100920. 1 hit.
SSF100934. SSF100934. 1 hit.
TIGRFAMsiTIGR02350. prok_dnaK. 1 hit.
PROSITEiPS00297. HSP70_1. 1 hit.
PS00329. HSP70_2. 1 hit.
PS01036. HSP70_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning of the hsp70 (dnaK) genes from Rhizobium meliloti and Pseudomonas cepacia: phylogenetic analyses of mitochondrial origin based on a highly conserved protein sequence."
    Falah M., Gupta R.S.
    J. Bacteriol. 176:7748-7753(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 25416.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1021.
  4. "Expression and regulation of phosphate stress inducible genes in Sinorhizobium meliloti."
    Summers M.L., Elkins J.G., Elliott B.A., McDermott T.R.
    Mol. Plant Microbe Interact. 11:1094-1101(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-101.
    Strain: 104A14.

Entry informationi

Entry nameiDNAK_RHIME
AccessioniPrimary (citable) accession number: P42374
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 2, 2001
Last modified: November 11, 2015
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.