P42347 (PI3K1_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Phosphatidylinositol 3-kinase, root isoform Short name=PI3-kinase Short name=PI3K Short name=PtdIns-3-kinase EC=2.7.1.137 Alternative name(s): SPI3K-5
Organism	Glycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier	3847 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	814 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Associated with membrane proliferation.
Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Induction	Repressed during nodule organogenesis and reinduced in mature nodules.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphatidylinositol-3-phosphate biosynthetic process Inferred from electronic annotation. Source: GOC phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 814	814	Phosphatidylinositol 3-kinase, root isoform		PRO_0000088820
Regions
	Domain	14 – 177	164	C2 PI3K-type
	Domain	274 – 449	176	PIK helical
	Domain	558 – 811	254	PI3K/PI4K

Sequences

	Sequence		Length	Mass (Da)
	P42347 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5B13A7DE16FFA0AD	FASTA	814	93,282
	10 20 30 40 50 60 MTGNEFRFFL SCDISVPVTF RVERLEGNLP LPNPKSPDLE TNAPTENRTK ELFVECALYI 70 80 90 100 110 120 DGAPFGLPTR TRLESSGPSY CWNELITLTT KYRDLTAQSQ LTFTVWDLSH GEGLIGGATI 130 140 150 160 170 180 LLFNNKKQLK TGKQKLRLWA GKEADGTFPT STPGKVPRHE RGELERLEKL VNKYERGQIQ 190 200 210 220 230 240 RVDWLDRLTF KTMERIKERE SLKNGSSHLY LVVDFCSFEH RVVFQESGAN FLFPSPIAST 250 260 270 280 290 300 NDIVVVWDPE VGKINPSEHK QLKLARSLTR GVIDRDLKPS SNERKSIQRI LKYPPTRTLS 310 320 330 340 350 360 GDERQLLWKF RFSLMSEKRA LTKFLRCVEW SDVQEAKQAL ELMGKWEMID VCDALELLSP 370 380 390 400 410 420 VFESEEVRAY AVSVLERADD EELQCYLLQL VQALRFERSD KSRLSHFLVQ RALRNIELAS 430 440 450 460 470 480 FLRWYVAVEL YDPAYAKRFY CTYEILEENM MKIAAGVNGE EDGFKQWQSL VRQTELTAQL 490 500 510 520 530 540 CSITREVRNV RGNTQKKIEK LRQLLSGLLS ELTYFDEPIR SPLAPGVLIA GIVPSESSIF 550 560 570 580 590 600 KSALHPLRLS FRTANGGTCK IIFKKGDDLR QDQLVVQMVS LMDRLLKLEN LDLHLTPYKV 610 620 630 640 650 660 LATGQDEGML EFIPSRSLAQ ILSENRSIIS YLQKFHPDDH GPFGITATCL ETFIKSCAGY 670 680 690 700 710 720 SVITYILGIG DRHLDNLLLR NDGGLFHVDF GFILGRDPKP FPPPMKLCKE MVEAMGGAES 730 740 750 760 770 780 QYYTRFKSYC CEAYNILRKS SNLILNLFYL MAGSNIPDIA SDPEKGILKL QEKFRLDLDD 790 800 810 EASIHFFQDL INESVSALFP QMVETIHRWA QYWR

References

[1]

"A phosphatidylinositol 3-kinase is induced during soybean nodule organogenesis and is associated with membrane proliferation." Hong Z., Verma D.P.S. Proc. Natl. Acad. Sci. U.S.A. 91:9617-9621(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Prize. Tissue: Root.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L27265 mRNA. Translation: AAA83995.1.
PIR	T07761.
RefSeq	NP_001236955.1. NM_001250026.2.
UniGene	Gma.2544.
3D structure databases
ProteinModelPortal	P42347.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	547983.
KEGG	dosa:Os05t0180600-02. gmx:547983.
Phylogenomic databases
KO	K00914.
Enzyme and pathway databases
BRENDA	2.7.1.137. 2483.
Gene expression databases
Genevestigator	P42347.
Family and domain databases
Gene3D	1.10.1070.11. 1 hit. 1.25.40.70. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR000008. C2_Ca-dep. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR011162. MHC_I/II-like_Ag-recog. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2_dom. IPR008290. PI3K_Vps34. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
PIRSF	PIRSF000587. PI3K_Vps34. 1 hit.
SMART	SM00239. C2. 1 hit. SM00142. PI3K_C2. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit. SSF54452. MHC_I/II-like_Ag-recog. 1 hit.
PROSITE	PS50004. C2. False negative. PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51547. PI3K_C2. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI3K1_SOYBN

Accession

Primary (citable) accession number: P42347

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	April 3, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program