P42339 (PI3K_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphatidylinositol 3-kinase VPS34
EC=2.7.1.137

Alternative name(s):
PI3-kinase VPS34
Short name=AtVPS34
Short name=PI3K VPS34
PtdIns-3-kinase VSP34

Gene names

Ordered Locus Names:	At1g60490
ORF Names:	F8A5.4

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	814 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.
Sequence similarities	Belongs to the PI3/PI4-kinase family. Contains 1 C2 PI3K-type domain. Contains 1 PI3K/PI4K domain. Contains 1 PIK helical domain.

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endocytosis Inferred from direct assay PubMed 17521408. Source: TAIR microgametogenesis Inferred from mutant phenotype PubMed 18408046. Source: TAIR phosphatidylinositol-3-phosphate biosynthetic process Inferred from electronic annotation. Source: GOC phosphatidylinositol-mediated signaling Inferred from electronic annotation. Source: InterPro reactive oxygen species metabolic process Inferred from mutant phenotype PubMed 17521408. Source: TAIR response to salt stress Inferred from mutant phenotype PubMed 17521408. Source: TAIR
Molecular_function	1-phosphatidylinositol-3-kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 814	814	Phosphatidylinositol 3-kinase VPS34		PRO_0000088819
Regions
	Domain	25 – 177	153	C2 PI3K-type
	Domain	274 – 449	176	PIK helical
	Domain	560 – 813	254	PI3K/PI4K
Experimental info
	Sequence conflict	37	1	S → A in AAA83427. Ref.1
	Sequence conflict	452 – 453	2	KL → NW in AAA83427. Ref.1
	Sequence conflict	557	1	G → R in AAA83427. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42339 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: 0A09926196E08D3E	FASTA	814	93,328
	10 20 30 40 50 60 MGANEFRFFL SCDINSPVTF RIEKLDGNLP VKKSSDSGVV SIAEEKKPEL YIECALYIDG 70 80 90 100 110 120 APFGLPMRTR LKTTGPPYCW NELITLSSKY RDLTAHSQLA ITVWDVSCGK TEGLIGGATV 130 140 150 160 170 180 LLFNSKMQMK SGKQKLRLWQ GKEADGSFPT STPGKVPRHE RGELERLEKL MNKYERGQIQ 190 200 210 220 230 240 SIDWLDRLML KSLDTIKEQE STKHGSSHLF VVIDFCSFEH RVVFQESGAN LFITAPIGST 250 260 270 280 290 300 NEFVTVWDTE LGKTNPSENK QLKLARSLDR GIIDRDLKPS NIERKSIQRV LKYPPTRTLS 310 320 330 340 350 360 GDERQLLWKF RFSLMSEKRA LTKFLRCVEW SDVQEAKQAI QLMYKWEMID VCDALELLSP 370 380 390 400 410 420 LFESEEVRAY AVSVLERADD EELQCYLLQL VQALRFERSD RSCLSQFLVQ RALQNIELAS 430 440 450 460 470 480 FLRWYVAVEL HDHVYAKRFY STYELLEENI IKLPPGVNGE DGYQLWQSLV RQTELTAQLC 490 500 510 520 530 540 SITREVRNVR GNTQKKIEKL RQLLGGLLSE LTYFEEPIRS PLTPNVLIKG IVAGESSLFK 550 560 570 580 590 600 SALHPLRLTF RTPEEGGSCK LIFKKGDDLR QDQLVVQMVW LMDRLLKLEN LDLCLTPYKV 610 620 630 640 650 660 LATGHDEGML EFIPSRSLAQ ILSEHRSITS YLQKFHPDEH APFGITATCL DTFIKSCAGY 670 680 690 700 710 720 SVITYILGIG DRHLDNLLLT DDGRLFHVDF AFILGRDPKP FPPPMKLCKE MVEAMGGAES 730 740 750 760 770 780 QYYTRFKSYC CEAYNILRKS SNLILNLFHL MAGSTIPDIA SDPEKGILKL QEKFRLDMDD 790 800 810 EACIHFFQDL INESVSALFP QMVETIHRWA QYWR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana, is an essential protein with homology to a calcium-dependent lipid binding domain." Welters P., Takegawa K., Emr S.D., Chrispeels M.J. Proc. Natl. Acad. Sci. U.S.A. 91:11398-11402(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Seedling.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U10669 mRNA. Translation: AAA83427.1. AC002292 Genomic DNA. Translation: AAB71971.1. CP002684 Genomic DNA. Translation: AEE33693.1. BT000878 mRNA. Translation: AAN41278.1.
IPI	IPI00517736.
PIR	B96630.
RefSeq	NP_176251.1. NM_104735.3.
UniGene	At.1545.
3D structure databases
ProteinModelPortal	P42339.
SMR	P42339. Positions 208-799.
ModBase	Search...
Protein-protein interaction databases
IntAct	P42339. 2 interactions.
Proteomic databases
PaxDb	P42339.
PRIDE	P42339.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G60490.1; AT1G60490.1; AT1G60490.
GeneID	842344.
KEGG	ath:AT1G60490.
Organism-specific databases
TAIR	At1g60490.
Phylogenomic databases
eggNOG	COG5032.
HOGENOM	HOG000174003.
InParanoid	P42339.
KO	K00914.
OMA	YLMVEFP.
PhylomeDB	P42339.
ProtClustDB	CLSN2681798.
Enzyme and pathway databases
BRENDA	2.7.1.137. 399.
Gene expression databases
ArrayExpress	P42339.
Genevestigator	P42339.
GermOnline	AT1G60490. Arabidopsis thaliana.
Family and domain databases
Gene3D	1.10.1070.11. 1 hit. 1.25.40.70. 1 hit.
InterPro	IPR016024. ARM-type_fold. IPR008973. C2_Ca/lipid-bd_dom_CaLB. IPR011009. Kinase-like_dom. IPR000403. PI3/4_kinase_cat_dom. IPR018936. PI3/4_kinase_CS. IPR002420. PI3K_C2_dom. IPR008290. PI3K_Vps34. IPR015433. PI_Kinase. IPR001263. PInositide-3_kin_accessory_dom. [Graphical view]
PANTHER	PTHR10048. PTHR10048. 1 hit.
Pfam	PF00454. PI3_PI4_kinase. 1 hit. PF00792. PI3K_C2. 1 hit. PF00613. PI3Ka. 1 hit. [Graphical view]
PIRSF	PIRSF000587. PI3K_Vps34. 1 hit.
SMART	SM00142. PI3K_C2. 1 hit. SM00145. PI3Ka. 1 hit. SM00146. PI3Kc. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit. SSF49562. C2_CaLB. 1 hit. SSF56112. Kinase_like. 1 hit.
PROSITE	PS00915. PI3_4_KINASE_1. 1 hit. PS00916. PI3_4_KINASE_2. 1 hit. PS50290. PI3_4_KINASE_3. 1 hit. PS51547. PI3K_C2. 1 hit. PS51545. PIK_HELICAL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PI3K_ARATH

Accession

Primary (citable) accession number: P42339
Secondary accession number(s): O22695

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 10, 2003
Last modified:	May 1, 2013
This is version 102 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program