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P42339

- PI3K_ARATH

UniProt

P42339 - PI3K_ARATH

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Protein

Phosphatidylinositol 3-kinase VPS34

Gene

At1g60490

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

GO - Molecular functioni

  1. 1-phosphatidylinositol-3-kinase activity Source: UniProtKB-EC
  2. ATP binding Source: UniProtKB-KW

GO - Biological processi

  1. endocytosis Source: TAIR
  2. microgametogenesis Source: TAIR
  3. phosphatidylinositol-mediated signaling Source: InterPro
  4. reactive oxygen species metabolic process Source: TAIR
  5. response to salt stress Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G60490-MONOMER.
BRENDAi2.7.1.137. 399.
ReactomeiREACT_187640. Synthesis of PIPs at the late endosome membrane.
REACT_187646. Synthesis of PIPs at the Golgi membrane.
REACT_187647. Synthesis of PIPs at the early endosome membrane.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol 3-kinase VPS34 (EC:2.7.1.137)
Alternative name(s):
PI3-kinase VPS34
Short name:
AtVPS34
Short name:
PI3K VPS34
PtdIns-3-kinase VSP34
Gene namesi
Ordered Locus Names:At1g60490
ORF Names:F8A5.4
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G60490.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 814814Phosphatidylinositol 3-kinase VPS34PRO_0000088819Add
BLAST

Proteomic databases

PaxDbiP42339.
PRIDEiP42339.

Expressioni

Gene expression databases

ExpressionAtlasiP42339. baseline and differential.
GenevestigatoriP42339.

Interactioni

Protein-protein interaction databases

BioGridi27568. 1 interaction.
IntActiP42339. 2 interactions.

Structurei

3D structure databases

ProteinModelPortaliP42339.
SMRiP42339. Positions 208-799.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini25 – 177153C2 PI3K-typePROSITE-ProRule annotationAdd
BLAST
Domaini274 – 449176PIK helicalPROSITE-ProRule annotationAdd
BLAST
Domaini560 – 813254PI3K/PI4KPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
Contains 1 PIK helical domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5032.
HOGENOMiHOG000174003.
InParanoidiP42339.
KOiK00914.
OMAiYLMVEFP.
PhylomeDBiP42339.

Family and domain databases

Gene3Di1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 2 hits.
InterProiIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERiPTHR10048. PTHR10048. 1 hit.
PfamiPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
SMARTiSM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42339-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGANEFRFFL SCDINSPVTF RIEKLDGNLP VKKSSDSGVV SIAEEKKPEL
60 70 80 90 100
YIECALYIDG APFGLPMRTR LKTTGPPYCW NELITLSSKY RDLTAHSQLA
110 120 130 140 150
ITVWDVSCGK TEGLIGGATV LLFNSKMQMK SGKQKLRLWQ GKEADGSFPT
160 170 180 190 200
STPGKVPRHE RGELERLEKL MNKYERGQIQ SIDWLDRLML KSLDTIKEQE
210 220 230 240 250
STKHGSSHLF VVIDFCSFEH RVVFQESGAN LFITAPIGST NEFVTVWDTE
260 270 280 290 300
LGKTNPSENK QLKLARSLDR GIIDRDLKPS NIERKSIQRV LKYPPTRTLS
310 320 330 340 350
GDERQLLWKF RFSLMSEKRA LTKFLRCVEW SDVQEAKQAI QLMYKWEMID
360 370 380 390 400
VCDALELLSP LFESEEVRAY AVSVLERADD EELQCYLLQL VQALRFERSD
410 420 430 440 450
RSCLSQFLVQ RALQNIELAS FLRWYVAVEL HDHVYAKRFY STYELLEENI
460 470 480 490 500
IKLPPGVNGE DGYQLWQSLV RQTELTAQLC SITREVRNVR GNTQKKIEKL
510 520 530 540 550
RQLLGGLLSE LTYFEEPIRS PLTPNVLIKG IVAGESSLFK SALHPLRLTF
560 570 580 590 600
RTPEEGGSCK LIFKKGDDLR QDQLVVQMVW LMDRLLKLEN LDLCLTPYKV
610 620 630 640 650
LATGHDEGML EFIPSRSLAQ ILSEHRSITS YLQKFHPDEH APFGITATCL
660 670 680 690 700
DTFIKSCAGY SVITYILGIG DRHLDNLLLT DDGRLFHVDF AFILGRDPKP
710 720 730 740 750
FPPPMKLCKE MVEAMGGAES QYYTRFKSYC CEAYNILRKS SNLILNLFHL
760 770 780 790 800
MAGSTIPDIA SDPEKGILKL QEKFRLDMDD EACIHFFQDL INESVSALFP
810
QMVETIHRWA QYWR
Length:814
Mass (Da):93,328
Last modified:January 10, 2003 - v2
Checksum:i0A09926196E08D3E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti37 – 371S → A in AAA83427. (PubMed:7972072)Curated
Sequence conflicti452 – 4532KL → NW in AAA83427. (PubMed:7972072)Curated
Sequence conflicti557 – 5571G → R in AAA83427. (PubMed:7972072)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10669 mRNA. Translation: AAA83427.1.
AC002292 Genomic DNA. Translation: AAB71971.1.
CP002684 Genomic DNA. Translation: AEE33693.1.
BT000878 mRNA. Translation: AAN41278.1.
PIRiB96630.
RefSeqiNP_176251.1. NM_104735.3.
UniGeneiAt.1545.

Genome annotation databases

EnsemblPlantsiAT1G60490.1; AT1G60490.1; AT1G60490.
GeneIDi842344.
KEGGiath:AT1G60490.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10669 mRNA. Translation: AAA83427.1 .
AC002292 Genomic DNA. Translation: AAB71971.1 .
CP002684 Genomic DNA. Translation: AEE33693.1 .
BT000878 mRNA. Translation: AAN41278.1 .
PIRi B96630.
RefSeqi NP_176251.1. NM_104735.3.
UniGenei At.1545.

3D structure databases

ProteinModelPortali P42339.
SMRi P42339. Positions 208-799.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 27568. 1 interaction.
IntActi P42339. 2 interactions.

Proteomic databases

PaxDbi P42339.
PRIDEi P42339.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G60490.1 ; AT1G60490.1 ; AT1G60490 .
GeneIDi 842344.
KEGGi ath:AT1G60490.

Organism-specific databases

TAIRi AT1G60490.

Phylogenomic databases

eggNOGi COG5032.
HOGENOMi HOG000174003.
InParanoidi P42339.
KOi K00914.
OMAi YLMVEFP.
PhylomeDBi P42339.

Enzyme and pathway databases

BioCyci ARA:AT1G60490-MONOMER.
BRENDAi 2.7.1.137. 399.
Reactomei REACT_187640. Synthesis of PIPs at the late endosome membrane.
REACT_187646. Synthesis of PIPs at the Golgi membrane.
REACT_187647. Synthesis of PIPs at the early endosome membrane.

Miscellaneous databases

PROi P42339.

Gene expression databases

ExpressionAtlasi P42339. baseline and differential.
Genevestigatori P42339.

Family and domain databases

Gene3Di 1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 2 hits.
InterProi IPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view ]
PANTHERi PTHR10048. PTHR10048. 1 hit.
Pfami PF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view ]
PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
SMARTi SM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana, is an essential protein with homology to a calcium-dependent lipid binding domain."
    Welters P., Takegawa K., Emr S.D., Chrispeels M.J.
    Proc. Natl. Acad. Sci. U.S.A. 91:11398-11402(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Seedling.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPI3K_ARATH
AccessioniPrimary (citable) accession number: P42339
Secondary accession number(s): O22695
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2003
Last modified: October 29, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3