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P42339

- PI3K_ARATH

UniProt

P42339 - PI3K_ARATH

Protein

Phosphatidylinositol 3-kinase VPS34

Gene

At1g60490

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (10 Jan 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

    GO - Molecular functioni

    1. 1-phosphatidylinositol-3-kinase activity Source: UniProtKB-EC
    2. ATP binding Source: UniProtKB-KW

    GO - Biological processi

    1. endocytosis Source: TAIR
    2. microgametogenesis Source: TAIR
    3. phosphatidylinositol-mediated signaling Source: InterPro
    4. reactive oxygen species metabolic process Source: TAIR
    5. response to salt stress Source: TAIR

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G60490-MONOMER.
    BRENDAi2.7.1.137. 399.
    ReactomeiREACT_187640. Synthesis of PIPs at the late endosome membrane.
    REACT_187646. Synthesis of PIPs at the Golgi membrane.
    REACT_187647. Synthesis of PIPs at the early endosome membrane.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphatidylinositol 3-kinase VPS34 (EC:2.7.1.137)
    Alternative name(s):
    PI3-kinase VPS34
    Short name:
    AtVPS34
    Short name:
    PI3K VPS34
    PtdIns-3-kinase VSP34
    Gene namesi
    Ordered Locus Names:At1g60490
    ORF Names:F8A5.4
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G60490.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 814814Phosphatidylinositol 3-kinase VPS34PRO_0000088819Add
    BLAST

    Proteomic databases

    PaxDbiP42339.
    PRIDEiP42339.

    Expressioni

    Gene expression databases

    ArrayExpressiP42339.
    GenevestigatoriP42339.

    Interactioni

    Protein-protein interaction databases

    BioGridi27568. 1 interaction.
    IntActiP42339. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP42339.
    SMRiP42339. Positions 208-799.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini25 – 177153C2 PI3K-typePROSITE-ProRule annotationAdd
    BLAST
    Domaini274 – 449176PIK helicalPROSITE-ProRule annotationAdd
    BLAST
    Domaini560 – 813254PI3K/PI4KPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the PI3/PI4-kinase family.PROSITE-ProRule annotation
    Contains 1 C2 PI3K-type domain.PROSITE-ProRule annotation
    Contains 1 PI3K/PI4K domain.PROSITE-ProRule annotation
    Contains 1 PIK helical domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5032.
    HOGENOMiHOG000174003.
    InParanoidiP42339.
    KOiK00914.
    OMAiYLMVEFP.
    PhylomeDBiP42339.

    Family and domain databases

    Gene3Di1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 2 hits.
    InterProiIPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view]
    PANTHERiPTHR10048. PTHR10048. 1 hit.
    PfamiPF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000587. PI3K_Vps34. 1 hit.
    SMARTiSM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42339-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGANEFRFFL SCDINSPVTF RIEKLDGNLP VKKSSDSGVV SIAEEKKPEL    50
    YIECALYIDG APFGLPMRTR LKTTGPPYCW NELITLSSKY RDLTAHSQLA 100
    ITVWDVSCGK TEGLIGGATV LLFNSKMQMK SGKQKLRLWQ GKEADGSFPT 150
    STPGKVPRHE RGELERLEKL MNKYERGQIQ SIDWLDRLML KSLDTIKEQE 200
    STKHGSSHLF VVIDFCSFEH RVVFQESGAN LFITAPIGST NEFVTVWDTE 250
    LGKTNPSENK QLKLARSLDR GIIDRDLKPS NIERKSIQRV LKYPPTRTLS 300
    GDERQLLWKF RFSLMSEKRA LTKFLRCVEW SDVQEAKQAI QLMYKWEMID 350
    VCDALELLSP LFESEEVRAY AVSVLERADD EELQCYLLQL VQALRFERSD 400
    RSCLSQFLVQ RALQNIELAS FLRWYVAVEL HDHVYAKRFY STYELLEENI 450
    IKLPPGVNGE DGYQLWQSLV RQTELTAQLC SITREVRNVR GNTQKKIEKL 500
    RQLLGGLLSE LTYFEEPIRS PLTPNVLIKG IVAGESSLFK SALHPLRLTF 550
    RTPEEGGSCK LIFKKGDDLR QDQLVVQMVW LMDRLLKLEN LDLCLTPYKV 600
    LATGHDEGML EFIPSRSLAQ ILSEHRSITS YLQKFHPDEH APFGITATCL 650
    DTFIKSCAGY SVITYILGIG DRHLDNLLLT DDGRLFHVDF AFILGRDPKP 700
    FPPPMKLCKE MVEAMGGAES QYYTRFKSYC CEAYNILRKS SNLILNLFHL 750
    MAGSTIPDIA SDPEKGILKL QEKFRLDMDD EACIHFFQDL INESVSALFP 800
    QMVETIHRWA QYWR 814
    Length:814
    Mass (Da):93,328
    Last modified:January 10, 2003 - v2
    Checksum:i0A09926196E08D3E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti37 – 371S → A in AAA83427. (PubMed:7972072)Curated
    Sequence conflicti452 – 4532KL → NW in AAA83427. (PubMed:7972072)Curated
    Sequence conflicti557 – 5571G → R in AAA83427. (PubMed:7972072)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10669 mRNA. Translation: AAA83427.1.
    AC002292 Genomic DNA. Translation: AAB71971.1.
    CP002684 Genomic DNA. Translation: AEE33693.1.
    BT000878 mRNA. Translation: AAN41278.1.
    PIRiB96630.
    RefSeqiNP_176251.1. NM_104735.3.
    UniGeneiAt.1545.

    Genome annotation databases

    EnsemblPlantsiAT1G60490.1; AT1G60490.1; AT1G60490.
    GeneIDi842344.
    KEGGiath:AT1G60490.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U10669 mRNA. Translation: AAA83427.1 .
    AC002292 Genomic DNA. Translation: AAB71971.1 .
    CP002684 Genomic DNA. Translation: AEE33693.1 .
    BT000878 mRNA. Translation: AAN41278.1 .
    PIRi B96630.
    RefSeqi NP_176251.1. NM_104735.3.
    UniGenei At.1545.

    3D structure databases

    ProteinModelPortali P42339.
    SMRi P42339. Positions 208-799.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 27568. 1 interaction.
    IntActi P42339. 2 interactions.

    Proteomic databases

    PaxDbi P42339.
    PRIDEi P42339.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G60490.1 ; AT1G60490.1 ; AT1G60490 .
    GeneIDi 842344.
    KEGGi ath:AT1G60490.

    Organism-specific databases

    TAIRi AT1G60490.

    Phylogenomic databases

    eggNOGi COG5032.
    HOGENOMi HOG000174003.
    InParanoidi P42339.
    KOi K00914.
    OMAi YLMVEFP.
    PhylomeDBi P42339.

    Enzyme and pathway databases

    BioCyci ARA:AT1G60490-MONOMER.
    BRENDAi 2.7.1.137. 399.
    Reactomei REACT_187640. Synthesis of PIPs at the late endosome membrane.
    REACT_187646. Synthesis of PIPs at the Golgi membrane.
    REACT_187647. Synthesis of PIPs at the early endosome membrane.

    Miscellaneous databases

    PROi P42339.

    Gene expression databases

    ArrayExpressi P42339.
    Genevestigatori P42339.

    Family and domain databases

    Gene3Di 1.10.1070.11. 1 hit.
    1.25.40.70. 1 hit.
    2.60.40.150. 2 hits.
    InterProi IPR016024. ARM-type_fold.
    IPR000008. C2_dom.
    IPR011009. Kinase-like_dom.
    IPR000403. PI3/4_kinase_cat_dom.
    IPR018936. PI3/4_kinase_CS.
    IPR002420. PI3K_C2_dom.
    IPR008290. PI3K_Vps34.
    IPR015433. PI_Kinase.
    IPR001263. PInositide-3_kin_accessory_dom.
    [Graphical view ]
    PANTHERi PTHR10048. PTHR10048. 1 hit.
    Pfami PF00454. PI3_PI4_kinase. 1 hit.
    PF00792. PI3K_C2. 1 hit.
    PF00613. PI3Ka. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000587. PI3K_Vps34. 1 hit.
    SMARTi SM00142. PI3K_C2. 1 hit.
    SM00145. PI3Ka. 1 hit.
    SM00146. PI3Kc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    SSF49562. SSF49562. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00915. PI3_4_KINASE_1. 1 hit.
    PS00916. PI3_4_KINASE_2. 1 hit.
    PS50290. PI3_4_KINASE_3. 1 hit.
    PS51547. PI3K_C2. 1 hit.
    PS51545. PIK_HELICAL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana, is an essential protein with homology to a calcium-dependent lipid binding domain."
      Welters P., Takegawa K., Emr S.D., Chrispeels M.J.
      Proc. Natl. Acad. Sci. U.S.A. 91:11398-11402(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Seedling.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPI3K_ARATH
    AccessioniPrimary (citable) accession number: P42339
    Secondary accession number(s): O22695
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 10, 2003
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3