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P42339 (PI3K_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 113. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphatidylinositol 3-kinase VPS34

EC=2.7.1.137
Alternative name(s):
PI3-kinase VPS34
Short name=AtVPS34
Short name=PI3K VPS34
PtdIns-3-kinase VSP34
Gene names
Ordered Locus Names:At1g60490
ORF Names:F8A5.4
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length814 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate.

Sequence similarities

Belongs to the PI3/PI4-kinase family.

Contains 1 C2 PI3K-type domain.

Contains 1 PI3K/PI4K domain.

Contains 1 PIK helical domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 814814Phosphatidylinositol 3-kinase VPS34
PRO_0000088819

Regions

Domain25 – 177153C2 PI3K-type
Domain274 – 449176PIK helical
Domain560 – 813254PI3K/PI4K

Experimental info

Sequence conflict371S → A in AAA83427. Ref.1
Sequence conflict452 – 4532KL → NW in AAA83427. Ref.1
Sequence conflict5571G → R in AAA83427. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P42339 [UniParc].

Last modified January 10, 2003. Version 2.
Checksum: 0A09926196E08D3E

FASTA81493,328
        10         20         30         40         50         60 
MGANEFRFFL SCDINSPVTF RIEKLDGNLP VKKSSDSGVV SIAEEKKPEL YIECALYIDG 

        70         80         90        100        110        120 
APFGLPMRTR LKTTGPPYCW NELITLSSKY RDLTAHSQLA ITVWDVSCGK TEGLIGGATV 

       130        140        150        160        170        180 
LLFNSKMQMK SGKQKLRLWQ GKEADGSFPT STPGKVPRHE RGELERLEKL MNKYERGQIQ 

       190        200        210        220        230        240 
SIDWLDRLML KSLDTIKEQE STKHGSSHLF VVIDFCSFEH RVVFQESGAN LFITAPIGST 

       250        260        270        280        290        300 
NEFVTVWDTE LGKTNPSENK QLKLARSLDR GIIDRDLKPS NIERKSIQRV LKYPPTRTLS 

       310        320        330        340        350        360 
GDERQLLWKF RFSLMSEKRA LTKFLRCVEW SDVQEAKQAI QLMYKWEMID VCDALELLSP 

       370        380        390        400        410        420 
LFESEEVRAY AVSVLERADD EELQCYLLQL VQALRFERSD RSCLSQFLVQ RALQNIELAS 

       430        440        450        460        470        480 
FLRWYVAVEL HDHVYAKRFY STYELLEENI IKLPPGVNGE DGYQLWQSLV RQTELTAQLC 

       490        500        510        520        530        540 
SITREVRNVR GNTQKKIEKL RQLLGGLLSE LTYFEEPIRS PLTPNVLIKG IVAGESSLFK 

       550        560        570        580        590        600 
SALHPLRLTF RTPEEGGSCK LIFKKGDDLR QDQLVVQMVW LMDRLLKLEN LDLCLTPYKV 

       610        620        630        640        650        660 
LATGHDEGML EFIPSRSLAQ ILSEHRSITS YLQKFHPDEH APFGITATCL DTFIKSCAGY 

       670        680        690        700        710        720 
SVITYILGIG DRHLDNLLLT DDGRLFHVDF AFILGRDPKP FPPPMKLCKE MVEAMGGAES 

       730        740        750        760        770        780 
QYYTRFKSYC CEAYNILRKS SNLILNLFHL MAGSTIPDIA SDPEKGILKL QEKFRLDMDD 

       790        800        810 
EACIHFFQDL INESVSALFP QMVETIHRWA QYWR 

« Hide

References

« Hide 'large scale' references
[1]"AtVPS34, a phosphatidylinositol 3-kinase of Arabidopsis thaliana, is an essential protein with homology to a calcium-dependent lipid binding domain."
Welters P., Takegawa K., Emr S.D., Chrispeels M.J.
Proc. Natl. Acad. Sci. U.S.A. 91:11398-11402(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Seedling.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U10669 mRNA. Translation: AAA83427.1.
AC002292 Genomic DNA. Translation: AAB71971.1.
CP002684 Genomic DNA. Translation: AEE33693.1.
BT000878 mRNA. Translation: AAN41278.1.
PIRB96630.
RefSeqNP_176251.1. NM_104735.3.
UniGeneAt.1545.

3D structure databases

ProteinModelPortalP42339.
SMRP42339. Positions 208-799.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid27568. 1 interaction.
IntActP42339. 2 interactions.

Proteomic databases

PaxDbP42339.
PRIDEP42339.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G60490.1; AT1G60490.1; AT1G60490.
GeneID842344.
KEGGath:AT1G60490.

Organism-specific databases

TAIRAT1G60490.

Phylogenomic databases

eggNOGCOG5032.
HOGENOMHOG000174003.
InParanoidP42339.
KOK00914.
OMAYLMVEFP.
PhylomeDBP42339.

Enzyme and pathway databases

BioCycARA:AT1G60490-MONOMER.
BRENDA2.7.1.137. 399.

Gene expression databases

ArrayExpressP42339.
GenevestigatorP42339.

Family and domain databases

Gene3D1.10.1070.11. 1 hit.
1.25.40.70. 1 hit.
2.60.40.150. 2 hits.
InterProIPR016024. ARM-type_fold.
IPR000008. C2_dom.
IPR011009. Kinase-like_dom.
IPR000403. PI3/4_kinase_cat_dom.
IPR018936. PI3/4_kinase_CS.
IPR002420. PI3K_C2_dom.
IPR008290. PI3K_Vps34.
IPR015433. PI_Kinase.
IPR001263. PInositide-3_kin_accessory_dom.
[Graphical view]
PANTHERPTHR10048. PTHR10048. 1 hit.
PfamPF00454. PI3_PI4_kinase. 1 hit.
PF00792. PI3K_C2. 1 hit.
PF00613. PI3Ka. 1 hit.
[Graphical view]
PIRSFPIRSF000587. PI3K_Vps34. 1 hit.
SMARTSM00142. PI3K_C2. 1 hit.
SM00145. PI3Ka. 1 hit.
SM00146. PI3Kc. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
SSF49562. SSF49562. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEPS00915. PI3_4_KINASE_1. 1 hit.
PS00916. PI3_4_KINASE_2. 1 hit.
PS50290. PI3_4_KINASE_3. 1 hit.
PS51547. PI3K_C2. 1 hit.
PS51545. PIK_HELICAL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP42339.

Entry information

Entry namePI3K_ARATH
AccessionPrimary (citable) accession number: P42339
Secondary accession number(s): O22695
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 10, 2003
Last modified: June 11, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names