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Protein

Inositol-trisphosphate 3-kinase B

Gene

Itpkb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 1D-myo-inositol 1,4,5-trisphosphate = ADP + 1D-myo-inositol 1,3,4,5-tetrakisphosphate.

Enzyme regulationi

IP3K is activated by calmodulin.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei667ATPBy similarity1
Binding sitei678ATPBy similarity1
Binding sitei731ATPBy similarity1
Binding sitei733SubstrateBy similarity1
Binding sitei754SubstrateBy similarity1
Binding sitei805ATPBy similarity1
Binding sitei885ATPBy similarity1
Binding sitei888SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi718 – 720ATPBy similarity3

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.1.127. 5301.
ReactomeiR-RNO-1855204. Synthesis of IP3 and IP4 in the cytosol.
SABIO-RKP42335.

Names & Taxonomyi

Protein namesi
Recommended name:
Inositol-trisphosphate 3-kinase B (EC:2.7.1.127)
Alternative name(s):
Inositol 1,4,5-trisphosphate 3-kinase B
Short name:
IP3 3-kinase B
Short name:
IP3K B
Short name:
InsP 3-kinase B
Gene namesi
Name:Itpkb
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 13

Organism-specific databases

RGDi2932. Itpkb.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000668691 – 934Inositol-trisphosphate 3-kinase BAdd BLAST934

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42PhosphoserineCombined sources1
Modified residuei48PhosphoserineCombined sources1
Modified residuei70PhosphoserineBy similarity1
Modified residuei209PhosphoserineCombined sources1
Modified residuei274PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP42335.
PRIDEiP42335.

PTM databases

iPTMnetiP42335.
PhosphoSitePlusiP42335.

Expressioni

Gene expression databases

ExpressionAtlasiP42335. baseline and differential.
GenevisibleiP42335. RN.

Interactioni

Subunit structurei

Interacts with DMTN.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004032.

Structurei

Secondary structure

1934
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni643 – 6453
Beta strandi647 – 65711
Beta strandi667 – 6704
Beta strandi675 – 6795
Helixi682 – 69110
Helixi697 – 6993
Beta strandi703 – 7097
Beta strandi712 – 7187
Turni720 – 7234
Beta strandi728 – 7369
Helixi741 – 7477
Helixi755 – 76410
Helixi771 – 7766
Helixi781 – 7888
Beta strandi791 – 7933
Helixi794 – 7974
Beta strandi798 – 8058
Helixi821 – 83212
Helixi836 – 85318
Helixi857 – 8604
Beta strandi862 – 8643
Beta strandi868 – 8736
Beta strandi879 – 8846
Beta strandi889 – 8913
Beta strandi900 – 9023
Helixi913 – 92917

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AQXX-ray2.50A/B642-930[»]
ProteinModelPortaliP42335.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42335.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni756 – 764Calmodulin-bindingBy similarity9
Regioni781 – 788Substrate bindingBy similarity8

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1621. Eukaryota.
ENOG410Y8AC. LUCA.
GeneTreeiENSGT00390000017438.
HOGENOMiHOG000060305.
HOVERGENiHBG052139.
InParanoidiP42335.
KOiK00911.
OMAiQGQFLGS.
OrthoDBiEOG091G03J3.
PhylomeDBiP42335.
TreeFamiTF318394.

Family and domain databases

InterProiIPR005522. IPK.
[Graphical view]
PANTHERiPTHR12400. PTHR12400. 2 hits.
PfamiPF03770. IPK. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42335-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAVYCYALNS LVIMNSTNEL KSGSPLPSGS ETPQPSGRAA LSPGSVFSPG
60 70 80 90 100
RGASFLFPPA ESLSLEEPGS AGGWRSGRRR LNSSSGSGGG SSSSNSSSSS
110 120 130 140 150
GVGSPSWAGR LRGDAQQVVA TRILSPPGPE EAQRKLRILQ RELQNVQVNQ
160 170 180 190 200
KVGMFEAQIQ AQTSAIQAPR SPRLGRARSP SPCPFRSSSQ PPERVLAPCS
210 220 230 240 250
PSEERRTKSW GEQCTETPDA NSRRRSRLST HPSKDKEGVA PLLGPASPTR
260 270 280 290 300
LGTQSPSTSV RMERGSPASP RCGSPTPMEI DKRTAPSLEH FGTSLTLATK
310 320 330 340 350
VAASAASAGP HPGHDSVLME ADCELGAMRP WEAHLERRGQ FLGRETGSAP
360 370 380 390 400
EPIRTHIREP PGRVERVHSV GGQGSWTPEV IKRPEEGTVD AQSSELSENP
410 420 430 440 450
RWSRLPGDPG SVGPEKGGSR IPGIRGPQQT LDSMREGSSA LGLLGGSQAA
460 470 480 490 500
QPGSMDVETG ISCGRMLEPL PPGEVTTNLK EPQCLPGDRM GMQPESSIVW
510 520 530 540 550
PSAVEEAPLI WTCDTGIQLK GTWRSQDGDA HPSCQEKSPD QKDKACSPSN
560 570 580 590 600
IPAIPAVIIT DMGAQEDGGL EEIQGSPRGP LPLRKLSSSS ASSTGFSSSY
610 620 630 640 650
EDSEEDISSD PERTLDPNSA FLHTLDQQKP RVSKSWRKIK NMVQWSPFVM
660 670 680 690 700
SFKKKYPWIQ LAGHAGSFKA AANGRILKKH CESEQRCLDR LMADVLRPFV
710 720 730 740 750
PAYHGDVVKD GERYNQMDDL LADFDSPCVM DCKMGIRTYL EEELTKARKK
760 770 780 790 800
PSLRKDMYQK MVEVDPEAPT EEEKAQRAVT KPRYMQWRET ISSTATLGFR
810 820 830 840 850
IEGIKKEDGS VNRDFKKTKT REQVTEAFRE FTKGNQNILI AYRDRLKAIR
860 870 880 890 900
ETLEVSPFFK CHEVIGSSLL FIHDKKEQAK VWMIDFGKTT PLPEGQTLQH
910 920 930
DVPWQEGNRE DGYLSGLNNL IDILTEMSQG SPLT
Length:934
Mass (Da):101,819
Last modified:July 19, 2004 - v3
Checksum:iC844B79747E1A117
GO

Sequence cautioni

The sequence CAA52298 differs from that shown. Reason: Frameshift at position 175.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63 – 68LSLEEP → ATKVPW (PubMed:8312366).Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242781 mRNA. Translation: CAC40660.1.
X74227 mRNA. Translation: CAA52298.1. Frameshift.
PIRiS41053.
RefSeqiNP_062185.2. NM_019312.2.
XP_017454406.1. XM_017598917.1.
XP_017454407.1. XM_017598918.1.
UniGeneiRn.22498.

Genome annotation databases

EnsembliENSRNOT00000004032; ENSRNOP00000004032; ENSRNOG00000002969.
GeneIDi54260.
KEGGirno:54260.
UCSCiRGD:2932. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ242781 mRNA. Translation: CAC40660.1.
X74227 mRNA. Translation: CAA52298.1. Frameshift.
PIRiS41053.
RefSeqiNP_062185.2. NM_019312.2.
XP_017454406.1. XM_017598917.1.
XP_017454407.1. XM_017598918.1.
UniGeneiRn.22498.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2AQXX-ray2.50A/B642-930[»]
ProteinModelPortaliP42335.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000004032.

PTM databases

iPTMnetiP42335.
PhosphoSitePlusiP42335.

Proteomic databases

PaxDbiP42335.
PRIDEiP42335.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000004032; ENSRNOP00000004032; ENSRNOG00000002969.
GeneIDi54260.
KEGGirno:54260.
UCSCiRGD:2932. rat.

Organism-specific databases

CTDi3707.
RGDi2932. Itpkb.

Phylogenomic databases

eggNOGiKOG1621. Eukaryota.
ENOG410Y8AC. LUCA.
GeneTreeiENSGT00390000017438.
HOGENOMiHOG000060305.
HOVERGENiHBG052139.
InParanoidiP42335.
KOiK00911.
OMAiQGQFLGS.
OrthoDBiEOG091G03J3.
PhylomeDBiP42335.
TreeFamiTF318394.

Enzyme and pathway databases

BRENDAi2.7.1.127. 5301.
ReactomeiR-RNO-1855204. Synthesis of IP3 and IP4 in the cytosol.
SABIO-RKP42335.

Miscellaneous databases

EvolutionaryTraceiP42335.
PROiP42335.

Gene expression databases

ExpressionAtlasiP42335. baseline and differential.
GenevisibleiP42335. RN.

Family and domain databases

InterProiIPR005522. IPK.
[Graphical view]
PANTHERiPTHR12400. PTHR12400. 2 hits.
PfamiPF03770. IPK. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIP3KB_RAT
AccessioniPrimary (citable) accession number: P42335
Secondary accession number(s): Q91XW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 19, 2004
Last modified: November 30, 2016
This is version 119 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.