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Protein

Rho GTPase-activating protein 25

Gene

ARHGAP25

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

GTPase activator for the Rho-type GTPases by converting them to an inactive GDP-bound state.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

GTPase activation

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163219-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.

Names & Taxonomyi

Protein namesi
Recommended name:
Rho GTPase-activating protein 25
Alternative name(s):
Rho-type GTPase-activating protein 25
Gene namesi
Name:ARHGAP25
Synonyms:KIAA0053
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:28951. ARHGAP25.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000163219.
PharmGKBiPA134941737.

Polymorphism and mutation databases

BioMutaiARHGAP25.
DMDMi238054314.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000567161 – 645Rho GTPase-activating protein 25Add BLAST645

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei362PhosphoserineCombined sources1
Modified residuei395PhosphoserineCombined sources1
Modified residuei406PhosphothreonineBy similarity1
Modified residuei536PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP42331.
PaxDbiP42331.
PeptideAtlasiP42331.
PRIDEiP42331.

PTM databases

iPTMnetiP42331.
PhosphoSitePlusiP42331.

Expressioni

Gene expression databases

BgeeiENSG00000163219.
CleanExiHS_ARHGAP25.
ExpressionAtlasiP42331. baseline and differential.
GenevisibleiP42331. HS.

Organism-specific databases

HPAiHPA035346.
HPA061395.

Interactioni

Protein-protein interaction databases

BioGridi115264. 29 interactors.
IntActiP42331. 2 interactors.
STRINGi9606.ENSP00000386911.

Structurei

Secondary structure

1645
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi47 – 56Combined sources10
Beta strandi58 – 61Combined sources4
Beta strandi63 – 71Combined sources9
Beta strandi74 – 82Combined sources9
Beta strandi87 – 90Combined sources4
Beta strandi95 – 100Combined sources6
Beta strandi111 – 117Combined sources7
Beta strandi129 – 132Combined sources4
Helixi136 – 151Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V89NMR-A47-151[»]
ProteinModelPortaliP42331.
SMRiP42331.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42331.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 151PHPROSITE-ProRule annotationAdd BLAST106
Domaini159 – 353Rho-GAPPROSITE-ProRule annotationAdd BLAST195

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili541 – 644Sequence analysisAdd BLAST104

Sequence similaritiesi

Contains 1 PH domain.PROSITE-ProRule annotation
Contains 1 Rho-GAP domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
GeneTreeiENSGT00760000118863.
HOGENOMiHOG000232151.
HOVERGENiHBG058875.
InParanoidiP42331.
KOiK20642.
OMAiGPGKKNS.
OrthoDBiEOG091G02DD.
PhylomeDBiP42331.
TreeFamiTF323577.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42331-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSLKLPRNWD FNLKVEAAKI ARSRSVMTGE QMAAFHPSST PNPLERPIKM
60 70 80 90 100
GWLKKQRSIV KNWQQRYFVL RAQQLYYYKD EEDTKPQGCM YLPGCTIKEI
110 120 130 140 150
ATNPEEAGKF VFEIIPASWD QNRMGQDSYV LMASSQAEME EWVKFLRRVA
160 170 180 190 200
GTPCGVFGQR LDETVAYEQK FGPHLVPILV EKCAEFILEH GRNEEGIFRL
210 220 230 240 250
PGQDNLVKQL RDAFDAGERP SFDRDTDVHT VASLLKLYLR DLPEPVVPWS
260 270 280 290 300
QYEGFLLCGQ LTNADEAKAQ QELMKQLSIL PRDNYSLLSY ICRFLHEIQL
310 320 330 340 350
NCAVNKMSVD NLATVIGVNL IRSKVEDPAV IMRGTPQIQR VMTMMIRDHE
360 370 380 390 400
VLFPKSKDIP LSPPAQKNDP KKAPVARSSV GWDATEDLRI SRTDSFSSMT
410 420 430 440 450
SDSDTTSPTG QQPSDAFPED SSKVPREKPG DWKMQSRKRT QTLPNRKCFL
460 470 480 490 500
TSAFQGANSS KMEIFKNEFW SPSSEAKAGE GHRRTMSQDL RQLSDSQRTS
510 520 530 540 550
TYDNVPSLPG SPGEEASALS SQACDSKGDT LASPNSETGP GKKNSGEEEI
560 570 580 590 600
DSLQRMVQEL RKEIETQKQM YEEQIKNLEK ENYDVWAKVV RLNEELEKEK
610 620 630 640
KKSAALEISL RNMERSREDV EKRNKALEEE VKEFVKSMKE PKTEA
Note: No experimental confirmation available.
Length:645
Mass (Da):73,435
Last modified:May 26, 2009 - v2
Checksum:i3D54323CA5571754
GO
Isoform 2 (identifier: P42331-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSLKLPRNWDFNLKVEAAK → MSLGQSACLFLS
     155-155: G → GA
     400-645: TSDSDTTSPT...KSMKEPKTEA → VRCREPSCFH...LSKSSFYWHL

Note: No experimental confirmation available.
Show »
Length:458
Mass (Da):51,779
Checksum:i73BC028BC8A2923E
GO
Isoform 3 (identifier: P42331-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSLKLPRNWDFNLKVEAAK → MSLGQSACLFLS

Show »
Length:638
Mass (Da):72,431
Checksum:i0B2897FB44880871
GO
Isoform 4 (identifier: P42331-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     155-155: G → GA

Show »
Length:646
Mass (Da):73,506
Checksum:i975E9D31C3740E42
GO
Isoform 5 (identifier: P42331-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     117-155: Missing.

Note: No experimental confirmation available.
Show »
Length:606
Mass (Da):69,017
Checksum:iC203F76F8BA05A6F
GO
Isoform 6 (identifier: P42331-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-19: MSLKLPRNWDFNLKVEAAK → MSLGQSACLFLS
     155-155: G → GA

Note: No experimental confirmation available. Gene prediction based on cDNA data.
Show »
Length:639
Mass (Da):72,502
Checksum:iA618E1940495D15D
GO

Sequence cautioni

The sequence BAA06125 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049142192R → W.Corresponds to variant rs3749130dbSNPEnsembl.1
Natural variantiVAR_049143555R → S.1 PublicationCorresponds to variant rs4241344dbSNPEnsembl.1
Natural variantiVAR_049144556M → T.1 PublicationCorresponds to variant rs10177248dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0373451 – 19MSLKL…VEAAK → MSLGQSACLFLS in isoform 2, isoform 3 and isoform 6. 2 PublicationsAdd BLAST19
Alternative sequenceiVSP_045391117 – 155Missing in isoform 5. 1 PublicationAdd BLAST39
Alternative sequenceiVSP_010275155G → GA in isoform 2, isoform 4 and isoform 6. 2 Publications1
Alternative sequenceiVSP_010276400 – 645TSDSD…PKTEA → VRCREPSCFHWVLPLVQAIP CKACSRVAIWGVLGDAVAVG AAATDSSEHTLKAWPLSKSS FYWHL in isoform 2. 1 PublicationAdd BLAST246

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29642 mRNA. Translation: BAA06125.2. Different initiation.
AK290396 mRNA. Translation: BAF83085.1.
AK297056 mRNA. Translation: BAH12484.1.
AC097495 Genomic DNA. No translation available.
AC105054 Genomic DNA. No translation available.
AC112700 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAW99866.1.
BC039591 mRNA. Translation: AAH39591.1.
CCDSiCCDS33214.2. [P42331-4]
CCDS46312.1. [P42331-3]
CCDS54363.1. [P42331-5]
CCDS54364.1. [P42331-6]
PIRiC59430.
RefSeqiNP_001007232.2. NM_001007231.2. [P42331-4]
NP_001159748.1. NM_001166276.1. [P42331-6]
NP_001159749.1. NM_001166277.1. [P42331-5]
NP_055697.1. NM_014882.2. [P42331-3]
XP_005264732.1. XM_005264675.2. [P42331-1]
UniGeneiHs.531807.

Genome annotation databases

EnsembliENST00000409030; ENSP00000386863; ENSG00000163219. [P42331-3]
ENST00000409202; ENSP00000386911; ENSG00000163219. [P42331-4]
ENST00000409220; ENSP00000386241; ENSG00000163219. [P42331-6]
ENST00000467265; ENSP00000420583; ENSG00000163219. [P42331-5]
ENST00000497079; ENSP00000417139; ENSG00000163219. [P42331-2]
GeneIDi9938.
KEGGihsa:9938.
UCSCiuc002sev.4. human. [P42331-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D29642 mRNA. Translation: BAA06125.2. Different initiation.
AK290396 mRNA. Translation: BAF83085.1.
AK297056 mRNA. Translation: BAH12484.1.
AC097495 Genomic DNA. No translation available.
AC105054 Genomic DNA. No translation available.
AC112700 Genomic DNA. No translation available.
CH471053 Genomic DNA. Translation: EAW99866.1.
BC039591 mRNA. Translation: AAH39591.1.
CCDSiCCDS33214.2. [P42331-4]
CCDS46312.1. [P42331-3]
CCDS54363.1. [P42331-5]
CCDS54364.1. [P42331-6]
PIRiC59430.
RefSeqiNP_001007232.2. NM_001007231.2. [P42331-4]
NP_001159748.1. NM_001166276.1. [P42331-6]
NP_001159749.1. NM_001166277.1. [P42331-5]
NP_055697.1. NM_014882.2. [P42331-3]
XP_005264732.1. XM_005264675.2. [P42331-1]
UniGeneiHs.531807.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V89NMR-A47-151[»]
ProteinModelPortaliP42331.
SMRiP42331.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi115264. 29 interactors.
IntActiP42331. 2 interactors.
STRINGi9606.ENSP00000386911.

PTM databases

iPTMnetiP42331.
PhosphoSitePlusiP42331.

Polymorphism and mutation databases

BioMutaiARHGAP25.
DMDMi238054314.

Proteomic databases

MaxQBiP42331.
PaxDbiP42331.
PeptideAtlasiP42331.
PRIDEiP42331.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000409030; ENSP00000386863; ENSG00000163219. [P42331-3]
ENST00000409202; ENSP00000386911; ENSG00000163219. [P42331-4]
ENST00000409220; ENSP00000386241; ENSG00000163219. [P42331-6]
ENST00000467265; ENSP00000420583; ENSG00000163219. [P42331-5]
ENST00000497079; ENSP00000417139; ENSG00000163219. [P42331-2]
GeneIDi9938.
KEGGihsa:9938.
UCSCiuc002sev.4. human. [P42331-1]

Organism-specific databases

CTDi9938.
GeneCardsiARHGAP25.
HGNCiHGNC:28951. ARHGAP25.
HPAiHPA035346.
HPA061395.
MIMi610587. gene.
neXtProtiNX_P42331.
OpenTargetsiENSG00000163219.
PharmGKBiPA134941737.
HUGEiSearch...
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4270. Eukaryota.
ENOG410XRR2. LUCA.
GeneTreeiENSGT00760000118863.
HOGENOMiHOG000232151.
HOVERGENiHBG058875.
InParanoidiP42331.
KOiK20642.
OMAiGPGKKNS.
OrthoDBiEOG091G02DD.
PhylomeDBiP42331.
TreeFamiTF323577.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000163219-MONOMER.
ReactomeiR-HSA-194840. Rho GTPase cycle.

Miscellaneous databases

EvolutionaryTraceiP42331.
GenomeRNAii9938.
PROiP42331.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000163219.
CleanExiHS_ARHGAP25.
ExpressionAtlasiP42331. baseline and differential.
GenevisibleiP42331. HS.

Family and domain databases

Gene3Di1.10.555.10. 1 hit.
2.30.29.30. 1 hit.
InterProiIPR011993. PH_dom-like.
IPR001849. PH_domain.
IPR008936. Rho_GTPase_activation_prot.
IPR000198. RhoGAP_dom.
[Graphical view]
PfamiPF00169. PH. 1 hit.
PF00620. RhoGAP. 1 hit.
[Graphical view]
SMARTiSM00233. PH. 1 hit.
SM00324. RhoGAP. 1 hit.
[Graphical view]
SUPFAMiSSF48350. SSF48350. 1 hit.
SSF50729. SSF50729. 1 hit.
PROSITEiPS50003. PH_DOMAIN. 1 hit.
PS50238. RHOGAP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRHG25_HUMAN
AccessioniPrimary (citable) accession number: P42331
Secondary accession number(s): A8K2Y1
, B7Z498, E9PFQ7, G5E9G2, Q8IXQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 26, 2009
Last modified: November 2, 2016
This is version 147 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.