P42329 (ALDH_GEOSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldehyde dehydrogenase, thermostable
EC=1.2.1.5

Gene names

Name:

aldHT

Organism

Geobacillus stearothermophilus (Bacillus stearothermophilus)

Taxonomic identifier

1422 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Geobacillus

Protein attributes

Sequence length	488 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidizes several aliphatic aldehydes, particularly c6-aliphatic aldehyde and hexanal, but does not oxidize benzaldehyde.
Catalytic activity	An aldehyde + NAD(P)+ + H2O = a carboxylate + NAD(P)H.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Subunit structure	Homotetramer.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.
Biophysicochemical properties	Temperature dependence: Optimum temperature is 55-60 degrees Celsius.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Molecular function	aldehyde dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 488	488	Aldehyde dehydrogenase, thermostable		PRO_0000056442
Regions
	Nucleotide binding	233 – 238	6	NAD By similarity
Sites
	Active site	255	1	By similarity
	Active site	289	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P42329 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 29E824451985D9ED	FASTA	488	52,915
	10 20 30 40 50 60 MKVQTEIKTY FNYINGNWVS SVSNNVEPSI NPANRHDIVG YVQRSTLEDV NEAVTAANEA 70 80 90 100 110 120 QTSWWKRSGV ERGEYLYKAA HILEQCLQDI AETMTREMGK TLAEAKAETM RGVHILRYYA 130 140 150 160 170 180 GEGARKIGDV IPSSDSEGLL FTTRVPLGVV GVISPWNFPV AIPIWKMAPA LVYGNTVVLK 190 200 210 220 230 240 PASETAVTAA KVIECFHEAG FPKGVVNMVC GSGSVVGQGI ANHPDIDGVT FTGSNTVGKQ 250 260 270 280 290 300 VGRAAFERGA KYQLEMGGKN PVIVAKDADL DLAVEGTISG GLRSTGQKCT ATSRVFIERE 310 320 330 340 350 360 VYEPFKAKLL ERVKQLKIGN GLDAETWMGP CASESQFHTV LSYIEKGKSE GAKLIYGGNR 370 380 390 400 410 420 CLEGELANGF FVEPTIFEDV DLQMTIAREE IFGPVLALIQ VDSIEEAIKL ANDTEYGLSA 430 440 450 460 470 480 SIYTKNIGNA LEFIKDIEAG LIKVNAETAG VEFQAPFGGM KQSSSHSREQ GQAAIEFFTS IKTVFVKA

References

[1]	"Cloning, nucleotide sequence, and efficient expression of the gene coding for thermostable aldehyde dehydrogenase from Bacillus stearothermophilus, and characterization of the enzyme." Imanaka T., Ohta T., Sakoda H., Widhyastuti N., Matsuoka M. J. Ferment. Bioeng. 76:161-167(1993) Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SIC1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D13846 Genomic DNA. Translation: BAA02975.1.
PIR	I39769.
3D structure databases
ProteinModelPortal	P42329.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. 1 hit. PS00687. ALDEHYDE_DEHYDR_GLU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALDH_GEOSE

Accession

Primary (citable) accession number: P42329

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program