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Protein

Alcohol dehydrogenase

Gene

adh

Organism
Geobacillus stearothermophilus (Bacillus stearothermophilus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Active with primary alcohols, including methanol.

Catalytic activityi

An alcohol + NAD+ = an aldehyde or ketone + NADH.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Enzyme regulationi

The rate-limiting step is NADH release. Catabolite repression.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi38Zinc 1; catalyticBy similarity1
Metal bindingi61Zinc 1; catalyticBy similarity1
Metal bindingi92Zinc 2By similarity1
Metal bindingi95Zinc 2By similarity1
Metal bindingi98Zinc 2By similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi148Zinc 1; catalyticBy similarity1
Binding sitei195NADBy similarity1
Binding sitei200NADBy similarity1
Binding sitei331NADBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi172 – 177NADBy similarity6
Nucleotide bindingi260 – 262NADBy similarity3

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandMetal-binding, NAD, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Alcohol dehydrogenase (EC:1.1.1.1)
Short name:
ADH
Gene namesi
Name:adh
OrganismiGeobacillus stearothermophilus (Bacillus stearothermophilus)
Taxonomic identifieri1422 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeGeobacillus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001607371 – 339Alcohol dehydrogenaseAdd BLAST339

Structurei

3D structure databases

ProteinModelPortaliP42327.
SMRiP42327.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SMARTiView protein in SMART
SM00829. PKS_ER. 1 hit.
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00059. ADH_ZINC. 1 hit.

Sequencei

Sequence statusi: Complete.

P42327-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKAAVVNEFK KALEIKEVER PKLEEGEVLV KIEACGVCHT DLHAAHGDWP
60 70 80 90 100
IKPKLPLIPG HEGVGIVVEV AKGVKSIKVG DRVGIPWLYS ACGECEYCLT
110 120 130 140 150
GQETLCPHQL NGGYSVDGGY AEYCKAPADY VAKIPDNLDP VEVAPILCAG
160 170 180 190 200
VTTYKALKVS GARPGEWVAI YGIGGLGHIA LQYAKAMGLN VVAVDISDEK
210 220 230 240 250
SKLAKDLGAD IAINGLKEDP VKAIHDQVGG VHAAISVAVN KKAFEQAYQS
260 270 280 290 300
VKRGGTLVVV GLPNADLPIP IFDTVLNGVS VKGSIVGTRK DMQEALDFAA
310 320 330
RGKVRPIVET AELEEINEVF ERMEKGKING RIVLKLKED
Length:339
Mass (Da):36,205
Last modified:November 1, 1995 - v1
Checksum:i0EC33CE7287D7476
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25544 Genomic DNA. Translation: CAA80989.1.
PIRiS47643.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z25544 Genomic DNA. Translation: CAA80989.1.
PIRiS47643.

3D structure databases

ProteinModelPortaliP42327.
SMRiP42327.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
3.90.180.10. 1 hit.
InterProiView protein in InterPro
IPR013149. ADH_C.
IPR013154. ADH_N.
IPR002328. ADH_Zn_CS.
IPR011032. GroES-like.
IPR016040. NAD(P)-bd_dom.
IPR020843. PKS_ER.
PfamiView protein in Pfam
PF08240. ADH_N. 1 hit.
PF00107. ADH_zinc_N. 1 hit.
SMARTiView protein in SMART
SM00829. PKS_ER. 1 hit.
SUPFAMiSSF50129. SSF50129. 1 hit.
SSF51735. SSF51735. 1 hit.
PROSITEiView protein in PROSITE
PS00059. ADH_ZINC. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiADH2_GEOSE
AccessioniPrimary (citable) accession number: P42327
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 15, 2017
This is version 86 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.