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Reviewed, UniProtKB/Swiss-Prot P42326 (TYSY1_BACSU)

Last modified February 9, 2010. Version 76. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Thymidylate synthase 1
      Short name=TS 1
      Short name=TSase 1
    EC=2.1.1.45
Alternative name(s):
    Thymidylate synthase A
      Short name=TS A
      Short name=TSase A
Gene names
Name: thyA1
Ordered Locus Names: BSU17680
OrganismBacillus subtilis [Complete proteome] [HAMAP]
Taxonomic identifier1423 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

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Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis. HAMAP MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP MF_00008

Subunit structure

Homodimer. HAMAP MF_00008

Subcellular location

Cytoplasm By similarity HAMAP MF_00008.

Miscellaneous

B.subtilis strain 168 possesses two thymidylate synthases, a major form thyA and a minor form thyB. HAMAP MF_00008

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type thyA subfamily.

Biophysicochemical properties

Temperature dependence:

Thermostable. Active at 46 degrees Celsius. HAMAP MF_00008

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Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processdTMP biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionthymidylate synthase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Thymidylate synthase 1 HAMAP MF_00008
PRO_0000140931

Sites

Active site1611 By similarity

Natural variations

Natural variant84 – 852ND → TE in strain: ATCC 6633 and W23.
Natural variant881M → K in strain: ATCC 6633 and W23.
Natural variant1181S → N in strain: ATCC 6633 and W23.
Natural variant1491N → S in strain: W23.
Natural variant1551A → S in strain: ATCC 6633 and W23.
Natural variant1711H → Q in strain: ATCC 6633 and W23.
Natural variant2651I → F in strain: W23.

Secondary structure

1 ................................................. 279
Helix Strand Turn

Details...

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Sequences

Sequence LengthMass (Da)Tools
P42326-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: CC0B127AAF7912BD

FASTA27932,807
        10         20         30         40         50         60 
MTQFDKQYNS IIKDIINNGI SDEEFDVRTK WDSDGTPAHT LSVISKQMRF DNSEVPILTT 

        70         80         90        100        110        120 
KKVAWKTAIK ELLWIWQLKS NDVNDLNMMG VHIWDQWKQE DGTIGHAYGF QLGKKNRSLN 

       130        140        150        160        170        180 
GEKVDQVDYL LHQLKNNPSS RRHITMLWNP DELDAMALTP CVYETQWYVK HGKLHLEVRA 

       190        200        210        220        230        240 
RSNDMALGNP FNVFQYNVLQ RMIAQVTGYE LGEYIFNIGD CHVYTRHIDN LKIQMEREQF 

       250        260        270 
EAPELWINPE VKDFYDFTID DFKLINYKHG DKLLFEVAV

« Hide

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References

« Hide 'large scale' references
[1]"The thyA gene from Bacillus subtilis exhibits similarity with the phage phi 3T thymidylate synthase gene."
Tam N.H., Borriss R.
Microbiology 141:291-297(1995) [PubMed: 7704257] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed: 9384377] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"Genes encoding thymidylate synthases A and B in the genus Bacillus are members of two distinct families."
Tam N.H., Borriss R.
Mol. Gen. Genet. 258:427-430(1998) [PubMed: 9648749] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 49-279.
Strain: ATCC 6633 / NCIMB 8054 / CCM1999 and W23.
[4]"Crystal structures of a unique thermal-stable thymidylate synthase from Bacillus subtilis."
Stout T.J., Schellenberger U., Santi D.V., Stroud R.M.
Biochemistry 37:14736-14747(1998) [PubMed: 9778348] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS).
[5]"Crystal structure of thymidylate synthase A from Bacillus subtilis."
Fox K.M., Maley F., Garibian A., Changchien L.M., van Roey P.
Protein Sci. 8:538-544(1999) [PubMed: 10091656] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X78560 Genomic DNA. Translation: CAA55307.1.
AL009126 Genomic DNA. Translation: CAB13652.1.
AF004102 Genomic DNA. Translation: AAC26324.1.
AF004103 Genomic DNA. Translation: AAC26325.1.
PIRI40494.
RefSeqNP_389651.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1B02X-ray2.50A1-279[»]
1BKOX-ray2.75A/B/C/D2-279[»]
1BKPX-ray1.70A/B2-279[»]
1BSFX-ray2.20A/B2-279[»]
1BSPX-ray2.50A/B2-279[»]
ModBaseSearch...

Genome annotation databases

GeneID939555.
GenomeReviewsGene locus BSU17680 in contig AL009126_GR.
KEGGbsu:BSU17680.
NMPDRfig|224308.1.peg.1772.

Organism-specific databases

SubtiListBG11190. thyA1. [Micado]
CMRSearch...

Phylogenomic databases

HOGENOMHBG588098.
OMADIKPEDF.
PhylomeDBP42326.

Enzyme and pathway databases

BRENDA2.1.1.45. 150.

Family and domain databases

HAMAPMF_00008. Thymidy_synth_bact.
[Tree]
InterProIPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
Gene3DG3DSA:3.30.572.10. Thymidylat_synth_C. 1 hit.
PANTHERPTHR11549:SF2. Thymidylat_synth_C. 1 hit.
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameTYSY1_BACSU
AccessionPrimary (citable) accession number: P42326
Secondary accession number(s): O30395, O30396
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 9, 2010
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents