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Protein

Neurocalcin homolog

Gene

Nca

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits the phosphorylation of rhodopsin in a calcium-dependent manner. Probably binds two or three calcium ions.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi73 – 84121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi109 – 120122PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi157 – 168123PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: FlyBase

GO - Biological processi

Complete GO annotation...

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

ReactomeiR-DME-451308. Activation of Ca-permeable Kainate Receptor.

Names & Taxonomyi

Protein namesi
Recommended name:
Neurocalcin homolog
Short name:
DrosNCa
Gene namesi
Name:Nca
ORF Names:CG7641
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0013303. Nca.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 190189Neurocalcin homologPRO_0000073801Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication

Keywords - PTMi

Lipoprotein, Myristate

Proteomic databases

PaxDbiP42325.

Expressioni

Tissue specificityi

Expressed in neuronal tissues. High level expression seen in the cortical regions of the central brain and lower levels in the lamina, the first optic lobe of the brain. It is also found in the thoracic ganglia.1 Publication

Developmental stagei

Found in the embryos, larvae and pupae. Expression in the adult heads is higher than in the bodies.1 Publication

Gene expression databases

BgeeiP42325.
ExpressionAtlasiP42325. differential.
GenevisibleiP42325. DM.

Interactioni

Protein-protein interaction databases

BioGridi65453. 16 interactions.
DIPiDIP-19235N.
IntActiP42325. 6 interactions.
MINTiMINT-304355.
STRINGi7227.FBpp0074671.

Structurei

3D structure databases

ProteinModelPortaliP42325.
SMRiP42325. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini23 – 5836EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini60 – 9536EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini96 – 13136EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini144 – 17936EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the recoverin family.Curated
Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
InParanoidiP42325.
OMAiMATHRSK.
OrthoDBiEOG7GJ6F3.
PhylomeDBiP42325.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGKQNSKLKP EVLEDLKQNT EFTDAEIQEW YKGFLKDCPS GHLSVEEFKK
60 70 80 90 100
IYGNFFPYGD ASKFAEHVFR TFDANGDGTI DFREFLCALS VTSRGKLEQK
110 120 130 140 150
LKWAFSMYDL DGNGYISRQE MLEIVTAIYK MVGSVMKMPE DESTPEKRTD
160 170 180 190
KIFRQMDRNK DGKLSLEEFI EGAKSDPSIV RLLQCDPQSH
Length:190
Mass (Da):21,894
Last modified:January 23, 2007 - v2
Checksum:iF1158FEB8CF13F64
GO

Mass spectrometryi

Molecular mass is 21975.51 Da from positions 2 - 190. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15735 mRNA. Translation: AAA62152.1.
AE014296 Genomic DNA. Translation: AAF49082.1.
AY071612 mRNA. Translation: AAL49234.1.
PIRiA55666.
RefSeqiNP_788543.1. NM_176365.3.
UniGeneiDm.6656.

Genome annotation databases

EnsemblMetazoaiFBtr0074902; FBpp0074671; FBgn0013303.
GeneIDi40186.
KEGGidme:Dmel_CG7641.
UCSCiCG7641-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U15735 mRNA. Translation: AAA62152.1.
AE014296 Genomic DNA. Translation: AAF49082.1.
AY071612 mRNA. Translation: AAL49234.1.
PIRiA55666.
RefSeqiNP_788543.1. NM_176365.3.
UniGeneiDm.6656.

3D structure databases

ProteinModelPortaliP42325.
SMRiP42325. Positions 5-185.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi65453. 16 interactions.
DIPiDIP-19235N.
IntActiP42325. 6 interactions.
MINTiMINT-304355.
STRINGi7227.FBpp0074671.

Proteomic databases

PaxDbiP42325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0074902; FBpp0074671; FBgn0013303.
GeneIDi40186.
KEGGidme:Dmel_CG7641.
UCSCiCG7641-RA. d. melanogaster.

Organism-specific databases

CTDi40186.
FlyBaseiFBgn0013303. Nca.

Phylogenomic databases

eggNOGiKOG0044. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000118820.
InParanoidiP42325.
OMAiMATHRSK.
OrthoDBiEOG7GJ6F3.
PhylomeDBiP42325.

Enzyme and pathway databases

ReactomeiR-DME-451308. Activation of Ca-permeable Kainate Receptor.

Miscellaneous databases

GenomeRNAii40186.
PROiP42325.

Gene expression databases

BgeeiP42325.
ExpressionAtlasiP42325. differential.
GenevisibleiP42325. DM.

Family and domain databases

Gene3Di1.10.238.10. 3 hits.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
IPR028846. Recoverin.
[Graphical view]
PANTHERiPTHR23055. PTHR23055. 1 hit.
PfamiPF00036. EF-hand_1. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 3 hits.
PS50222. EF_HAND_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A highly conserved homologue of bovine neurocalcin in Drosophila melanogaster is a Ca(2+)-binding protein expressed in neuronal tissues."
    Teng D.H.-F., Chen C.-K., Hurley J.B.
    J. Biol. Chem. 269:31900-31907(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Canton-S.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Drosophila neurocalcin, a fatty acylated, Ca2+-binding protein that associates with membranes and inhibits in vitro phosphorylation of bovine rhodopsin."
    Faurobert E., Chen C.-K., Hurley J.B., Teng D.H.-F.
    J. Biol. Chem. 271:10256-10262(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MASS SPECTROMETRY, MYRISTOYLATION AT GLY-2.

Entry informationi

Entry nameiNCAH_DROME
AccessioniPrimary (citable) accession number: P42325
Secondary accession number(s): Q9VW67
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.