Reviewed, UniProtKB/Swiss-Prot P42325 (NCAH_DROME)

Names and origin

Protein names

Recommended name:
    Neurocalcin homolog
      Short name=DrosNCa

Gene names

Name:	Nca
ORF Names:	CG7641

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	190 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Inhibits the phosphorylation of rhodopsin in a calcium-dependent manner. Probably binds two or three calcium ions. Ref.1 Ref.5
Tissue specificity	Expressed in neuronal tissues. High level expression seen in the cortical regions of the central brain and lower levels in the lamina, the first optic lobe of the brain. It is also found in the thoracic ganglia. Ref.1
Developmental stage	Found in the embryos, larvae and pupae. Expression in the adult heads is higher than in the bodies. Ref.1
Sequence similarities	Belongs to the recoverin family. Contains 4 EF-hand domains.
Mass spectrometry	Molecular mass is 21975.51 Da from positions 2 - 190. Determined by ESI. Ref.5

Ontologies

Keywords
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	calcium ion binding Ref.1 Inferred from direct assay. Source: FlyBase protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
	Q8INH9	1	EBI-149848,EBI-112777
	Q9VVV3	1	EBI-149848,EBI-155653
	Q9W3I4	1	EBI-149848,EBI-129482
babo	Q8MKS1	1	EBI-149848,EBI-103891
Pvf3	Q9VM39	1	EBI-149848,EBI-143185
RpLP0	P19889	1	EBI-149848,EBI-195497

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 190	189	Neurocalcin homolog		PRO_0000073801
Regions
	Domain	23 – 58	36	EF-hand 1
	Domain	60 – 95	36	EF-hand 2
	Domain	96 – 131	36	EF-hand 3
	Domain	144 – 179	36	EF-hand 4
	Calcium binding	73 – 84	12	1 Potential
	Calcium binding	109 – 120	12	2 Potential
	Calcium binding	157 – 168	12	3 Potential
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine

Sequences

	Sequence		Length	Mass (Da)
	P42325-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: F1158FEB8CF13F64	FASTA	190	21,894
	10 20 30 40 50 60 MGKQNSKLKP EVLEDLKQNT EFTDAEIQEW YKGFLKDCPS GHLSVEEFKK IYGNFFPYGD 70 80 90 100 110 120 ASKFAEHVFR TFDANGDGTI DFREFLCALS VTSRGKLEQK LKWAFSMYDL DGNGYISRQE 130 140 150 160 170 180 MLEIVTAIYK MVGSVMKMPE DESTPEKRTD KIFRQMDRNK DGKLSLEEFI EGAKSDPSIV 190 RLLQCDPQSH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A highly conserved homologue of bovine neurocalcin in Drosophila melanogaster is a Ca(2+)-binding protein expressed in neuronal tissues." Teng D.H.-F., Chen C.-K., Hurley J.B. J. Biol. Chem. 269:31900-31907(1994) [PubMed: 7989365] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Canton-S.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Drosophila neurocalcin, a fatty acylated, Ca2+-binding protein that associates with membranes and inhibits in vitro phosphorylation of bovine rhodopsin." Faurobert E., Chen C.-K., Hurley J.B., Teng D.H.-F. J. Biol. Chem. 271:10256-10262(1996) [PubMed: 8626592] [Abstract] Cited for: FUNCTION, MASS SPECTROMETRY, MYRISTOYLATION AT GLY-2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U15735 mRNA. Translation: AAA62152.1. AE014296 Genomic DNA. Translation: AAF49082.1. AY071612 mRNA. Translation: AAL49234.1.
PIR	A55666.
RefSeq	NP_788543.1.
UniGene	Dm.6656
3D structure databases
HSSP	HSSP built from PDB template 1BJF based on UniProtKB P29554.
SMR	P42325. Positions 5-185.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:19235N.
IntAct	P42325. 16 interactions.
Genome annotation databases
Ensembl	FBgn0013303. Drosophila melanogaster. [Contig view]
GeneID	40186.
KEGG	dme:Dmel_CG7641.
NMPDR	fig|7227.3.peg.10652.
Organism-specific databases
FlyBase	FBgn0013303. Nca.
Phylogenomic databases
HOGENOM	P42325.
OMA	P42325. DCPSGNL.
Gene expression databases
GermOnline	CG7641. Drosophila melanogaster.
Family and domain databases
InterPro	IPR011992. EF-Hand_type. IPR018248. EF_hand. IPR018247. EF_HAND_1. IPR018249. EF_HAND_2. IPR002048. EF_hand_Ca_bd. IPR001125. Recoverin. [Graphical view]
Gene3D	G3DSA:1.10.238.10. EF-Hand_type. 1 hit.
Pfam	PF00036. efhand. 3 hits. [Graphical view]
PRINTS	PR00450. RECOVERIN.
ProDom	PD000012. EF-hand. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00054. EFh. 3 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 3 hits. PS50222. EF_HAND_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	817463.

Entry information

Entry name

NCAH_DROME

Accession

Primary (citable) accession number: P42325
Secondary accession number(s): Q9VW67

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	June 16, 2009
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project