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Protein

Protein gurken

Gene

grk

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Critical for defining the anterior-posterior and dorsal-ventral axes of the egg. May signal directly to dorsal follicle cells through the receptor torpedo (top). During oogenesis this signaling pathway instructs follicle cells to follow a dorsal pathway of development rather than the default ventral pathway.1 Publication

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: FlyBase

GO - Biological processi

  • anterior/posterior axis specification, follicular epithelium Source: FlyBase
  • anterior/posterior pattern specification Source: FlyBase
  • border follicle cell migration Source: FlyBase
  • cargo loading into COPII-coated vesicle Source: UniProtKB
  • cell fate determination Source: FlyBase
  • cell fate specification Source: FlyBase
  • chorion-containing eggshell pattern formation Source: FlyBase
  • dorsal/ventral axis specification, ovarian follicular epithelium Source: FlyBase
  • dorsal appendage formation Source: FlyBase
  • embryonic axis specification Source: FlyBase
  • epidermal growth factor receptor signaling pathway Source: FlyBase
  • gurken signaling pathway Source: FlyBase
  • maternal determination of dorsal/ventral axis, ovarian follicular epithelium, germ-line encoded Source: FlyBase
  • oocyte anterior/posterior axis specification Source: FlyBase
  • oocyte axis specification Source: FlyBase
  • oocyte dorsal/ventral axis specification Source: FlyBase
  • oocyte microtubule cytoskeleton organization Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Oogenesis

Enzyme and pathway databases

SignaLinkiP42287

Names & Taxonomyi

Protein namesi
Recommended name:
Protein gurken
Gene namesi
Name:grk
ORF Names:CG17610
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0001137 grk

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini27 – 247ExtracellularSequence analysisAdd BLAST221
Transmembranei248 – 268HelicalSequence analysisAdd BLAST21
Topological domaini269 – 295CytoplasmicSequence analysisAdd BLAST27

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 26Sequence analysisAdd BLAST26
ChainiPRO_000000760727 – 295Protein gurkenAdd BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi183 ↔ 198PROSITE-ProRule annotation
Glycosylationi188N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi192 ↔ 212PROSITE-ProRule annotation
Glycosylationi205N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi214 ↔ 223PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP42287
PRIDEiP42287

Miscellaneous databases

PMAP-CutDBiP42287

Expressioni

Tissue specificityi

Expressed in nurse cells and oocyte up to oogenesis stage 7. Specifically accumulates in dorsal anterior corner of the oocyte during stages 9/10, at later stages expression is seen as an anterior ring. In stage 10 ovaries, it is concentrated between the oocyte nucleus and the adjacent oolemma. During vitellogenesis stage it can be detected at the oocyte surface, especially on the microvilli. It is also found at the microvilli covering the apical surface of the follicular epithelium and within follicle cells.2 Publications

Gene expression databases

BgeeiFBgn0001137
GenevisibleiP42287 DM

Interactioni

Subunit structurei

Interacts with cni.1 Publication

GO - Molecular functioni

  • epidermal growth factor receptor binding Source: FlyBase

Protein-protein interaction databases

BioGridi60293, 60 interactors
DIPiDIP-20922N
IntActiP42287, 31 interactors
STRINGi7227.FBpp0079313

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5F5KX-ray2.40B241-246[»]
ProteinModelPortaliP42287
SMRiP42287
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini179 – 224EGF-likePROSITE-ProRule annotationAdd BLAST46

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni215 – 245Interaction with cniAdd BLAST31

Keywords - Domaini

EGF-like domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410KC03 Eukaryota
ENOG4110MRC LUCA
InParanoidiP42287
KOiK02447
OMAiMHEHAHE
OrthoDBiEOG091G0IXE
PhylomeDBiP42287

Family and domain databases

InterProiView protein in InterPro
IPR013032 EGF-like_CS
IPR000742 EGF-like_dom
PROSITEiView protein in PROSITE
PS00022 EGF_1, 1 hit
PS50026 EGF_3, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMQIPFTRIF KVIFVLSTIV AVTDCCSSRI LLLREHTLKI VQHQHSHMHE
60 70 80 90 100
HAHELQQQIQ ETAVELLNRL ELQRKQLEAS AQEEADQLHP DTDPNPDSGG
110 120 130 140 150
QLPNADDSIA ADPEQDGIIL GSSTDTWLAS ESSTPITDSE TVTTPETVTH
160 170 180 190 200
TGEPPPDPSS SSTPDSTTPS PNDKETEIQM LPCSEAYNTS FCLNGGHCFQ
210 220 230 240 250
HPMVNNTVFH SCLCVNDYDG ERCAYKSWNG DYIYSPPTAQ RKVRMAHIVF
260 270 280 290
SFPVLLMLSS LYVLFAAVFM LRNVPDYRRK QQQLHLHKQR FFVRC
Length:295
Mass (Da):33,280
Last modified:January 17, 2003 - v2
Checksum:i80E993704DE3123B
GO

Sequence cautioni

The sequence AAA28598 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAF72000 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22531 mRNA Translation: AAA28598.1 Different initiation.
AF223394 Genomic DNA Translation: AAF72000.1 Different initiation.
AE014134 Genomic DNA Translation: AAF52675.4
AY051814 mRNA Translation: AAK93238.1
PIRiA48844
RefSeqiNP_476568.2, NM_057220.3
UniGeneiDm.355

Genome annotation databases

EnsemblMetazoaiFBtr0079708; FBpp0079313; FBgn0001137
GeneIDi34171
KEGGidme:Dmel_CG17610
UCSCiCG17610-RA d. melanogaster

Similar proteinsi

Entry informationi

Entry nameiGRK_DROME
AccessioniPrimary (citable) accession number: P42287
Secondary accession number(s): Q9VLL2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 17, 2003
Last modified: March 28, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health