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Protein

Trypsin zeta

Gene

zetaTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei87 – 871Charge relay systemBy similarity
Active sitei134 – 1341Charge relay systemBy similarity
Sitei228 – 2281Required for specificityBy similarity
Active sitei234 – 2341Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: FlyBase

GO - Biological processi

  1. proteolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.116.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin zeta (EC:3.4.21.4)
Gene namesi
Name:zetaTry
ORF Names:CG12387
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0011556. zetaTry.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222CuratedAdd
BLAST
Propeptidei23 – 3816Activation peptidePRO_0000028287Add
BLAST
Chaini39 – 280242Trypsin zetaPRO_0000028288Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi72 ↔ 88PROSITE-ProRule annotation
Disulfide bondi198 ↔ 218PROSITE-ProRule annotation
Disulfide bondi230 ↔ 254PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP42280.

Expressioni

Gene expression databases

BgeeiP42280.

Interactioni

Protein-protein interaction databases

BioGridi62009. 2 interactions.
IntActiP42280. 2 interactions.
MINTiMINT-297272.
STRINGi7227.FBpp0087260.

Structurei

3D structure databases

ProteinModelPortaliP42280.
SMRiP42280. Positions 39-274.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 278240Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00770000120824.
InParanoidiP42280.
KOiK01312.
OMAiVSNELCD.
OrthoDBiEOG75B84T.
PhylomeDBiP42280.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42280-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSSWIVGLL AFLVSLVALT QGLPLLEDLD EKSVPDGRIV GGYATDIAQV
60 70 80 90 100
PYQISLRYKG ITTPENPFRH RCGGSIFNET TIVTAAHCVI GTVASQYKVV
110 120 130 140 150
AGTNFQTGSD GVITNVKEIV MHEGYYSGAA YNNDIAILFV DPPLPLNNFT
160 170 180 190 200
IKAIKLALEQ PIEGTVSKVS GWGTTSPGGY SSNQLLAVDV PIVSNELCDQ
210 220 230 240 250
DYEDFGDETY RITSAMLCAG KRGVGGADAC QGDSGGPLAV RDELYGVVSW
260 270 280
GNSCALPNYP GVYANVAYLR PWIDAVLAGL
Length:280
Mass (Da):29,738
Last modified:August 16, 2005 - v2
Checksum:i99A2D76557C308FC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti86 – 861A → G in AAA17456 (PubMed:10486967).Curated
Sequence conflicti145 – 1451P → A in AAA17456 (PubMed:10486967).Curated
Sequence conflicti153 – 1531A → G in AAA17456 (PubMed:10486967).Curated
Sequence conflicti158 – 1581L → S in AAA17456 (PubMed:10486967).Curated
Sequence conflicti222 – 2221R → P in AAA17456 (PubMed:10486967).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04853 Genomic DNA. Translation: AAA17456.1.
AE013599 Genomic DNA. Translation: AAF58663.1.
AY113523 mRNA. Translation: AAM29528.1.
RefSeqiNP_523691.1. NM_078967.4.
UniGeneiDm.2633.

Genome annotation databases

EnsemblMetazoaiFBtr0088164; FBpp0087260; FBgn0011556.
GeneIDi36216.
KEGGidme:Dmel_CG12387.
UCSCiCG12387-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04853 Genomic DNA. Translation: AAA17456.1.
AE013599 Genomic DNA. Translation: AAF58663.1.
AY113523 mRNA. Translation: AAM29528.1.
RefSeqiNP_523691.1. NM_078967.4.
UniGeneiDm.2633.

3D structure databases

ProteinModelPortaliP42280.
SMRiP42280. Positions 39-274.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62009. 2 interactions.
IntActiP42280. 2 interactions.
MINTiMINT-297272.
STRINGi7227.FBpp0087260.

Protein family/group databases

MEROPSiS01.116.

Proteomic databases

PaxDbiP42280.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088164; FBpp0087260; FBgn0011556.
GeneIDi36216.
KEGGidme:Dmel_CG12387.
UCSCiCG12387-RA. d. melanogaster.

Organism-specific databases

CTDi36216.
FlyBaseiFBgn0011556. zetaTry.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00770000120824.
InParanoidiP42280.
KOiK01312.
OMAiVSNELCD.
OrthoDBiEOG75B84T.
PhylomeDBiP42280.

Miscellaneous databases

GenomeRNAii36216.
NextBioi797384.

Gene expression databases

BgeeiP42280.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTRYZ_DROME
AccessioniPrimary (citable) accession number: P42280
Secondary accession number(s): Q9V5X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 16, 2005
Last modified: February 4, 2015
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.