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Protein

Trypsin eta

Gene

etaTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei74 – 741Charge relay systemBy similarity
Active sitei120 – 1201Charge relay systemBy similarity
Sitei209 – 2091Required for specificityBy similarity
Active sitei215 – 2151Charge relay systemBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.117.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin eta (EC:3.4.21.4)
Gene namesi
Name:etaTry
ORF Names:CG12386
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0011554. etaTry.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Propeptidei23 – 275Activation peptidePRO_0000028283
Chaini28 – 262235Trypsin etaPRO_0000028284Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi59 ↔ 75PROSITE-ProRule annotation
Disulfide bondi185 ↔ 200PROSITE-ProRule annotation
Disulfide bondi211 ↔ 235PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP42279.

Expressioni

Gene expression databases

BgeeiP42279.
ExpressionAtlasiP42279. differential.
GenevisibleiP42279. DM.

Interactioni

Protein-protein interaction databases

BioGridi62010. 1 interaction.
MINTiMINT-317423.
STRINGi7227.FBpp0087259.

Structurei

3D structure databases

ProteinModelPortaliP42279.
SMRiP42279. Positions 28-260.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini28 – 259232Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00790000123162.
InParanoidiP42279.
KOiK01312.
OMAiYWRPISE.
OrthoDBiEOG75B84T.
PhylomeDBiP42279.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42279-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNKVILRILA VLFLLGIYAV SAQSDGRIVG GADTSSYYTK YVVQLRRRSS
60 70 80 90 100
SSSSYAQTCG GCILDAVTIA TAAHCVYNRE AENFLVVAGD DSRGGMNGVV
110 120 130 140 150
VRVSKLIPHE LYNSSTMDND IALVVVDPPL PLDSFSTMEA IEIASEQPAV
160 170 180 190 200
GVQATISGWG YTKENGLSSD QLQQVKVPIV DSEKCQEAYY WRPISEGMLC
210 220 230 240 250
AGLSEGGKDA CQGDSGGPLV VANKLAGIVS WGEGCARPNY PGVYANVAYY
260
KDWIAKQRTS YV
Length:262
Mass (Da):28,088
Last modified:August 16, 2005 - v2
Checksum:iBE908F7602A611DC
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti8 – 81I → V in AAA17455 (PubMed:10486967).Curated
Sequence conflicti24 – 241S → P in AAA17455 (PubMed:10486967).Curated
Sequence conflicti88 – 881A → S in AAA17455 (PubMed:10486967).Curated
Sequence conflicti97 – 971N → Y in AAA17455 (PubMed:10486967).Curated
Sequence conflicti105 – 1051K → Q in AAA17455 (PubMed:10486967).Curated
Sequence conflicti142 – 1421E → V in AAA17455 (PubMed:10486967).Curated
Sequence conflicti149 – 1491A → P in AAA17455 (PubMed:10486967).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04853 Genomic DNA. Translation: AAA17455.1.
AE013599 Genomic DNA. Translation: AAF58662.1.
BT012306 mRNA. Translation: AAS77431.1.
RefSeqiNP_523692.1. NM_078968.2.
UniGeneiDm.23664.

Genome annotation databases

EnsemblMetazoaiFBtr0088163; FBpp0087259; FBgn0011554.
GeneIDi36217.
KEGGidme:Dmel_CG12386.
UCSCiCG12386-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04853 Genomic DNA. Translation: AAA17455.1.
AE013599 Genomic DNA. Translation: AAF58662.1.
BT012306 mRNA. Translation: AAS77431.1.
RefSeqiNP_523692.1. NM_078968.2.
UniGeneiDm.23664.

3D structure databases

ProteinModelPortaliP42279.
SMRiP42279. Positions 28-260.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62010. 1 interaction.
MINTiMINT-317423.
STRINGi7227.FBpp0087259.

Protein family/group databases

MEROPSiS01.117.

Proteomic databases

PaxDbiP42279.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088163; FBpp0087259; FBgn0011554.
GeneIDi36217.
KEGGidme:Dmel_CG12386.
UCSCiCG12386-RA. d. melanogaster.

Organism-specific databases

CTDi36217.
FlyBaseiFBgn0011554. etaTry.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00790000123162.
InParanoidiP42279.
KOiK01312.
OMAiYWRPISE.
OrthoDBiEOG75B84T.
PhylomeDBiP42279.

Miscellaneous databases

GenomeRNAii36217.
NextBioi797389.
PROiP42279.

Gene expression databases

BgeeiP42279.
ExpressionAtlasiP42279. differential.
GenevisibleiP42279. DM.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M., Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.
    Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-262.
    Strain: Berkeley.
    Tissue: Larva and Pupae.

Entry informationi

Entry nameiTRYU_DROME
AccessioniPrimary (citable) accession number: P42279
Secondary accession number(s): Q6NLM5, Q9V5X9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 16, 2005
Last modified: June 24, 2015
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.