Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Trypsin theta

Gene

thetaTry

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761Charge relay systemBy similarity
Active sitei121 – 1211Charge relay systemBy similarity
Sitei210 – 2101Required for specificityBy similarity
Active sitei216 – 2161Charge relay systemBy similarity

GO - Molecular functioni

  1. serine-type endopeptidase activity Source: FlyBase

GO - Biological processi

  1. proteolysis Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.114.

Names & Taxonomyi

Protein namesi
Recommended name:
Trypsin theta (EC:3.4.21.4)
Gene namesi
Name:thetaTry
ORF Names:CG12385
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0011555. thetaTry.

Subcellular locationi

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919CuratedAdd
BLAST
Propeptidei20 – 3415Activation peptidePRO_0000028279Add
BLAST
Chaini35 – 262228Trypsin thetaPRO_0000028280Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi61 ↔ 77PROSITE-ProRule annotation
Disulfide bondi186 ↔ 203PROSITE-ProRule annotation
Disulfide bondi212 ↔ 236PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Zymogen

Proteomic databases

PaxDbiP42278.
PRIDEiP42278.

Expressioni

Gene expression databases

BgeeiP42278.
ExpressionAtlasiP42278. differential.

Structurei

3D structure databases

ProteinModelPortaliP42278.
SMRiP42278. Positions 35-255.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini35 – 260226Peptidase S1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation
Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00620000088101.
InParanoidiP42278.
KOiK01312.
OMAiYIELATE.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP42278.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42278-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MHRLVVLLVC LAVGSACAGT VGVSNGDPFE REGRIVGGED TTIGAHPYQV
60 70 80 90 100
SLQTKSGSHF CGGSLINEDT VVTAAHCLVG RKVSKVFVRL GSTLYNEGGI
110 120 130 140 150
VVAVRELAYN EDYNSKTMEY DVGILKLDEK VKETENIRYI ELATETPPTG
160 170 180 190 200
TTAVVTGWGS KCYFWCMTLP KTLQEVYVNI VDWKTCASDE YKYGEIIYDS
210 220 230 240 250
MVCAYEKKKD ACQGDSGGPL AVGNTLVGIV SWGYACASNL LPGVYSDVPA
260
LRKWILNASE TL
Length:262
Mass (Da):28,378
Last modified:August 16, 2005 - v2
Checksum:i440A66F07037C985
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti45 – 462AH → GD in AAA17454. (PubMed:10486967)Curated
Sequence conflicti73 – 731T → A in AAL48054. (PubMed:12537569)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04853 Genomic DNA. Translation: AAA17454.1.
AE013599 Genomic DNA. Translation: AAF58661.1.
AY070583 mRNA. Translation: AAL48054.1.
RefSeqiNP_523693.1. NM_078969.5.
UniGeneiDm.5696.

Genome annotation databases

EnsemblMetazoaiFBtr0088162; FBpp0087258; FBgn0011555.
GeneIDi36218.
KEGGidme:Dmel_CG12385.
UCSCiCG12385-RA. d. melanogaster.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U04853 Genomic DNA. Translation: AAA17454.1.
AE013599 Genomic DNA. Translation: AAF58661.1.
AY070583 mRNA. Translation: AAL48054.1.
RefSeqiNP_523693.1. NM_078969.5.
UniGeneiDm.5696.

3D structure databases

ProteinModelPortaliP42278.
SMRiP42278. Positions 35-255.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiS01.114.

Proteomic databases

PaxDbiP42278.
PRIDEiP42278.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0088162; FBpp0087258; FBgn0011555.
GeneIDi36218.
KEGGidme:Dmel_CG12385.
UCSCiCG12385-RA. d. melanogaster.

Organism-specific databases

CTDi36218.
FlyBaseiFBgn0011555. thetaTry.

Phylogenomic databases

eggNOGiCOG5640.
GeneTreeiENSGT00620000088101.
InParanoidiP42278.
KOiK01312.
OMAiYIELATE.
OrthoDBiEOG7MKW6Q.
PhylomeDBiP42278.

Miscellaneous databases

GenomeRNAii36218.
NextBioi797394.

Gene expression databases

BgeeiP42278.
ExpressionAtlasiP42278. differential.

Family and domain databases

InterProiIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamiPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSiPR00722. CHYMOTRYPSIN.
SMARTiSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMiSSF50494. SSF50494. 1 hit.
PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Concerted evolution within a trypsin gene cluster in Drosophila."
    Wang S., Magoulas C., Hickey D.A.
    Mol. Biol. Evol. 16:1117-1124(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Oregon-R.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.

Entry informationi

Entry nameiTRYT_DROME
AccessioniPrimary (citable) accession number: P42278
Secondary accession number(s): Q8SZQ7, Q9V5Y0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: August 16, 2005
Last modified: February 4, 2015
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.