Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

2-oxo-hept-4-ene-1,7-dioate hydratase

Gene

hpcG

Organism
Escherichia coli
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Transforms 2-oxo-hept-4-ene-1,7-dioate (OHED) into 4-hydroxy-2-oxoheptanedioate, a step in the 4-hydroxyphenylacetic acid (4-HPA) degradation pathway.1 Publication

Catalytic activityi

(4Z)-2-oxohept-4-enedioate + H2O = (4S)-4-hydroxy-2-oxoheptanedioate.2 Publications

Cofactori

Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication

Pathwayi: 4-hydroxyphenylacetate degradation

This protein is involved in step 6 of the subpathway that synthesizes pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate.
Proteins known to be involved in the 7 steps of the subpathway in this organism are:
  1. 4-hydroxyphenylacetate 3-monooxygenase oxygenase component (hpaB), 4-hydroxyphenylacetate 3-monooxygenase reductase component (hpaC)
  2. 3,4-dihydroxyphenylacetate 2,3-dioxygenase (hpcB)
  3. 5-carboxymethyl-2-hydroxymuconate semialdehyde dehydrogenase (hpcC)
  4. 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase (hpaG), 5-carboxymethyl-2-hydroxymuconate Delta-isomerase (hpcD), Homoprotocatechuate catabolism bifunctional isomerase/decarboxylase (hpcE)
  5. 4-hydroxyphenylacetate degradation bifunctional isomerase/decarboxylase (hpaG), Homoprotocatechuate catabolism bifunctional isomerase/decarboxylase (hpcE)
  6. 2-oxo-hept-4-ene-1,7-dioate hydratase (hpcG)
  7. 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (yfaU), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpaI), 4-hydroxy-2-oxo-heptane-1,7-dioate aldolase (hpcH)
This subpathway is part of the pathway 4-hydroxyphenylacetate degradation, which is itself part of Aromatic compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate, the pathway 4-hydroxyphenylacetate degradation and in Aromatic compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi106MagnesiumCombined sources1 Publication1
Metal bindingi108MagnesiumCombined sources1 Publication1
Metal bindingi139MagnesiumCombined sources1 Publication1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Aromatic hydrocarbons catabolism

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00208; UER00421.

Names & Taxonomyi

Protein namesi
Recommended name:
2-oxo-hept-4-ene-1,7-dioate hydratase1 Publication (EC:4.2.1.1632 Publications)
Short name:
OHED hydratase1 Publication
Gene namesi
Name:hpcG1 Publication
OrganismiEscherichia coli
Taxonomic identifieri562 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000840421 – 2672-oxo-hept-4-ene-1,7-dioate hydrataseAdd BLAST267

Proteomic databases

PRIDEiP42270.

Interactioni

Subunit structurei

Homodecamer.1 Publication

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EB4X-ray1.60A/B/C/D/E1-267[»]
2EB5X-ray1.70A/B/C/D/E1-267[»]
2EB6X-ray1.69A/B/C/D/E1-267[»]
ProteinModelPortaliP42270.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the hydratase/decarboxylase family.Curated

Family and domain databases

Gene3Di3.90.850.10. 1 hit.
InterProiIPR011234. Fumarylacetoacetase_C-rel.
IPR012690. HpcG.
[Graphical view]
PfamiPF01557. FAA_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56529. SSF56529. 1 hit.
TIGRFAMsiTIGR02312. HpaH. 1 hit.

Sequencei

Sequence statusi: Complete.

P42270-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFDKHTHTLI AQRLDQAEKQ REQIRAISLD YPEITIEDAY AVQREWVRLK
60 70 80 90 100
IAEGRTLKGH KIGLTSKAMQ ASSQISEPDY GALLDDMFFH DGSDIPTDRF
110 120 130 140 150
IVPRIEVELA FVLAKPLRGP NCTLFDVYNA TDYVIPALEL IDARCHNIDP
160 170 180 190 200
ETQRPRKVFD TISDNAANAG VILGGRPIKP DELDLRWISA LMYRNGVIEE
210 220 230 240 250
TGVAAGVLNH PANGVAWLAN KLAPYDVQLE AGQIILGGSF TRPVPARKGD
260
TFHVDYGNMG SISCRFV
Length:267
Mass (Da):29,715
Last modified:October 5, 2016 - v2
Checksum:iADA88A33D8A1079E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti85 – 98DDMFF…DIPTD → HDNVLPRWQRY in CAA57202 (PubMed:7737515).CuratedAdd BLAST14
Sequence conflicti169A → S in CAA57202 (PubMed:7737515).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81446 Genomic DNA. Translation: CAA57202.1.
AF036583 Genomic DNA. Translation: AAB91474.1.
PIRiI41083.
RefSeqiWP_000459750.1. NZ_MBSX01000016.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X81446 Genomic DNA. Translation: CAA57202.1.
AF036583 Genomic DNA. Translation: AAB91474.1.
PIRiI41083.
RefSeqiWP_000459750.1. NZ_MBSX01000016.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2EB4X-ray1.60A/B/C/D/E1-267[»]
2EB5X-ray1.70A/B/C/D/E1-267[»]
2EB6X-ray1.69A/B/C/D/E1-267[»]
ProteinModelPortaliP42270.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP42270.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00208; UER00421.

Family and domain databases

Gene3Di3.90.850.10. 1 hit.
InterProiIPR011234. Fumarylacetoacetase_C-rel.
IPR012690. HpcG.
[Graphical view]
PfamiPF01557. FAA_hydrolase. 1 hit.
[Graphical view]
SUPFAMiSSF56529. SSF56529. 1 hit.
TIGRFAMsiTIGR02312. HpaH. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPCG_ECOLX
AccessioniPrimary (citable) accession number: P42270
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 5, 2016
Last modified: November 30, 2016
This is version 63 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.