Reviewed, UniProtKB/Swiss-Prot P42255 (ABFB_ASPNG)

Names and origin

Protein names

Recommended name:
    Alpha-N-arabinofuranosidase B
      Short name=Arabinosidase B
      Short name=ABF B
    EC=3.2.1.55

Gene names

Name:

abfB

Organism

Aspergillus niger

Taxonomic identifier

5061 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Aspergillus

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Able to hydrolyze 1,5-, 1,3- and 1,2-alpha-linkages not only in L-arabinofuranosyl oligosaccharides, but also in polysac-charides containing terminal non-reducing L-arabinofuranoses in side chains, like L-arabinan, arabinogalactan and arabinoxylan.
Catalytic activity	Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides.
Pathway	Glycan metabolism; L-arabinan degradation.
Induction	By growth on polymeric substrates and L-arabitol.
Sequence similarities	Belongs to the glycosyl hydrolase 54 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	L-arabinose metabolic process Inferred from electronic annotation. Source: InterPro arabinan metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	alpha-N-arabinofuranosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.1
	Chain	19 – 499	481	Alpha-N-arabinofuranosidase B		PRO_0000012223
Amino acid modifications
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	202	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P42255-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: F1F6C3B47AA419C9	FASTA	499	52,523
	10 20 30 40 50 60 MFSRRNLVAL GLAATVSAGP CDIYEAGDTP CVAAHSTTRA LYSSFSGALY QLQRGSDDTT 70 80 90 100 110 120 TTISPLTAGG VADASAQDTF CANTTCLITI IYDQSGNGNH LTQAPPGGFD GPDVDGYDNL 130 140 150 160 170 180 ASAIGAPVTL NGQKAYGVFM SPGTGYRNNE ATGTATGDEP EGMYAVLDGT HYNDACCFDY 190 200 210 220 230 240 GNAETSSTDT GAGHMEAIYL GNSTTWGYGA GDGPWIMVDM ENNLFSGADE GYNSGDPSIS 250 260 270 280 290 300 YSFVTAAVKG GADKWAIRGG NAASGSLSTY YSGARPDYSG YNPMSKEGAI ILGIGGDNSN 310 320 330 340 350 360 GAQGTFYEGV MTSGYPSDDV ENSVQENIVA AKYVSGSLVS GPSFTSGEVV SLRVTTPGYT 370 380 390 400 410 420 TRYIAHTDTT VNTQVVDDDS STTLKEEASW TVVTGLANSQ CFSFESVDTP GSYIRHYNFE 430 440 450 460 470 480 LLLNANDGTK QFHEDATFCP QAPLNGEGTS LRSWSYPTRY FRHYENVLYA ASNGGVQTFD 490 SKTSFNNDVS FEIETAFAS

References

[1]

"Cloning and characterization of the abfB gene coding for the major alpha-L-arabinofuranosidase (ABF B) of Aspergillus niger." Flipphi M.J.A., van Heuvel M., van der Veen P., Visser J., de Graaff L.H. Curr. Genet. 24:525-532(1993) [PubMed: 8299175] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 19-30; 221-234; 285-304 AND 312-324. Strain: ATCC 9029 / NRRL 3 / CBS 120.49 / DSM 2466 / N400.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L23502 Genomic DNA. Translation: AAB53944.1.
PIR	S39113.
3D structure databases
SMR	P42255. Positions 19-499.
ModBase	Search...
Protein family/group databases
CAZy	CBM42. Carbohydrate-Binding Module Family 42. GH54. Glycoside Hydrolase Family 54.
Enzyme and pathway databases
BRENDA	3.2.1.55. 277.
Family and domain databases
InterPro	IPR015289. A-L-arabinofuranosidase_B_cat. IPR007934. AbfB. IPR013320. ConA-like_subgrp. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF05270. AbfB. 1 hit. PF09206. ArabFuran-catal. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ABFB_ASPNG

Accession

Primary (citable) accession number: P42255

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)