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Protein

Alkaline phosphatase D

Gene

phoD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciBSUB:BSU02620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase D (EC:3.1.3.1)
Short name:
APaseD
Gene namesi
Name:phoD
Synonyms:ycbS
Ordered Locus Names:BSU02620
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 56Tat-type signal1 PublicationAdd BLAST56
ChainiPRO_000002208857 – 583Alkaline phosphatase DAdd BLAST527

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Proteomic databases

PaxDbiP42251.

Expressioni

Inductioni

By phosphate starvation.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001458.

Structurei

Secondary structure

1583
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi69 – 72Combined sources4
Beta strandi79 – 84Combined sources6
Helixi89 – 91Combined sources3
Beta strandi100 – 109Combined sources10
Beta strandi112 – 123Combined sources12
Helixi125 – 127Combined sources3
Beta strandi130 – 135Combined sources6
Beta strandi143 – 150Combined sources8
Beta strandi158 – 161Combined sources4
Beta strandi173 – 178Combined sources6
Helixi183 – 185Combined sources3
Helixi189 – 194Combined sources6
Beta strandi200 – 204Combined sources5
Beta strandi228 – 231Combined sources4
Helixi235 – 246Combined sources12
Helixi249 – 257Combined sources9
Beta strandi258 – 262Combined sources5
Turni267 – 269Combined sources3
Helixi284 – 301Combined sources18
Helixi306 – 308Combined sources3
Beta strandi319 – 323Combined sources5
Turni324 – 326Combined sources3
Beta strandi327 – 331Combined sources5
Beta strandi334 – 337Combined sources4
Helixi342 – 344Combined sources3
Beta strandi345 – 348Combined sources4
Helixi352 – 355Combined sources4
Helixi364 – 376Combined sources13
Beta strandi379 – 385Combined sources7
Beta strandi398 – 400Combined sources3
Helixi408 – 410Combined sources3
Helixi412 – 424Combined sources13
Beta strandi430 – 434Combined sources5
Beta strandi436 – 448Combined sources13
Beta strandi456 – 462Combined sources7
Beta strandi473 – 475Combined sources3
Helixi479 – 485Combined sources7
Beta strandi489 – 494Combined sources6
Beta strandi496 – 504Combined sources9
Beta strandi507 – 516Combined sources10
Beta strandi518 – 521Combined sources4
Beta strandi526 – 533Combined sources8
Beta strandi540 – 545Combined sources6
Helixi560 – 576Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YEQX-ray1.93A/B57-583[»]
ProteinModelPortaliP42251.
SMRiP42251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PhoD family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105DGI. Bacteria.
COG3540. LUCA.
HOGENOMiHOG000240657.
InParanoidiP42251.
KOiK01113.
OMAiYRGYVRC.
PhylomeDBiP42251.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR018946. Alk_phosphatase_PhoD-like.
IPR029052. Metallo-depent_PP-like.
IPR032093. PhoD_N.
[Graphical view]
PfamiPF09423. PhoD. 1 hit.
PF16655. PhoD_N. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42251-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAYDSRFDEW VQKLKEESFQ NNTFDRRKFI QGAGKIAGLS LGLTIAQSVG
60 70 80 90 100
AFEVNAAPNF SSYPFTLGVA SGDPLSDSVV LWTRLAPDPL NGGGMPKQAV
110 120 130 140 150
PVKWEVAKDE HFRKIVRKGT EMAKPSLAHS VHVEADGLEP NKVYYYRFKT
160 170 180 190 200
GHELSPVGKT KTLPAPGANV PQMTFAFASC QQYEHGYYTA YKHMAKEKLD
210 220 230 240 250
LVFHLGDYIY EYGPNEYVSK TGNVRTHNSA EIITLQDYRN RHAQYRSDAN
260 270 280 290 300
LKAAHAAFPW VVTWDDHEVE NNYANKIPEK GQSVEAFVLR RAAAYQAYYE
310 320 330 340 350
HMPLRISSLP NGPDMQLYRH FTYGNLASFN VLDTRQYRDD QANNDGNKPP
360 370 380 390 400
SDESRNPNRT LLGKEQEQWL FNNLGSSTAH WNVLAQQIFF AKWNFGTSAS
410 420 430 440 450
PIYSMDSWDG YPAQRERVIN FIKSKNLNNV VVLTGDVHAS WASNLHVDFE
460 470 480 490 500
KTSSKIFGAE FVGTSITSGG NGADKRADTD QILKENPHIQ FFNDYRGYVR
510 520 530 540 550
CTVTPHQWKA DYRVMPFVTE PGAAISTRAS FVYQKDQTGL RKVSSTTIQG
560 570 580
GVKQSDEVEE DRFFSHNKAH EKQMIKKRAK ITN
Length:583
Mass (Da):65,971
Last modified:June 16, 2009 - v3
Checksum:i7734224CB2470319
GO

Sequence cautioni

The sequence AAB47803 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.Curated
The sequence BAA06483 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.Curated

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti59N → K.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49060 Genomic DNA. Translation: AAB47803.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB12056.2.
D30808 Genomic DNA. Translation: BAA06483.1. Frameshift.
PIRiD69676.
RefSeqiNP_388144.2. NC_000964.3.
WP_009969242.1. NZ_JNCM01000030.1.

Genome annotation databases

EnsemblBacteriaiCAB12056; CAB12056; BSU02620.
GeneIDi938391.
KEGGibsu:BSU02620.
PATRICi18972083. VBIBacSub10457_0270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U49060 Genomic DNA. Translation: AAB47803.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB12056.2.
D30808 Genomic DNA. Translation: BAA06483.1. Frameshift.
PIRiD69676.
RefSeqiNP_388144.2. NC_000964.3.
WP_009969242.1. NZ_JNCM01000030.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2YEQX-ray1.93A/B57-583[»]
ProteinModelPortaliP42251.
SMRiP42251.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100001458.

Proteomic databases

PaxDbiP42251.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB12056; CAB12056; BSU02620.
GeneIDi938391.
KEGGibsu:BSU02620.
PATRICi18972083. VBIBacSub10457_0270.

Phylogenomic databases

eggNOGiENOG4105DGI. Bacteria.
COG3540. LUCA.
HOGENOMiHOG000240657.
InParanoidiP42251.
KOiK01113.
OMAiYRGYVRC.
PhylomeDBiP42251.

Enzyme and pathway databases

BioCyciBSUB:BSU02620-MONOMER.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR018946. Alk_phosphatase_PhoD-like.
IPR029052. Metallo-depent_PP-like.
IPR032093. PhoD_N.
[Graphical view]
PfamiPF09423. PhoD. 1 hit.
PF16655. PhoD_N. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPPBD_BACSU
AccessioniPrimary (citable) accession number: P42251
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2009
Last modified: November 2, 2016
This is version 110 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.