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P42251 (PPBD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase D

Short name=APaseD
EC=3.1.3.1
Gene names
Name:phoD
Synonyms:ycbS
Ordered Locus Names:BSU02620
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length583 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Induction

By phosphate starvation.

Post-translational modification

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Sequence similarities

Belongs to the PhoD family.

Sequence caution

The sequence AAB47803.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.

The sequence BAA06483.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.

Ontologies

Keywords
   Biological processStress response
   DomainSignal
   Molecular functionHydrolase
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processresponse to stress

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionalkaline phosphatase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5656Tat-type signal Ref.1
Chain57 – 583527Alkaline phosphatase D
PRO_0000022088

Natural variations

Natural variant591N → K.

Secondary structure

..................................................................................... 583
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P42251 [UniParc].

Last modified June 16, 2009. Version 3.
Checksum: 7734224CB2470319

FASTA58365,971
        10         20         30         40         50         60 
MAYDSRFDEW VQKLKEESFQ NNTFDRRKFI QGAGKIAGLS LGLTIAQSVG AFEVNAAPNF 

        70         80         90        100        110        120 
SSYPFTLGVA SGDPLSDSVV LWTRLAPDPL NGGGMPKQAV PVKWEVAKDE HFRKIVRKGT 

       130        140        150        160        170        180 
EMAKPSLAHS VHVEADGLEP NKVYYYRFKT GHELSPVGKT KTLPAPGANV PQMTFAFASC 

       190        200        210        220        230        240 
QQYEHGYYTA YKHMAKEKLD LVFHLGDYIY EYGPNEYVSK TGNVRTHNSA EIITLQDYRN 

       250        260        270        280        290        300 
RHAQYRSDAN LKAAHAAFPW VVTWDDHEVE NNYANKIPEK GQSVEAFVLR RAAAYQAYYE 

       310        320        330        340        350        360 
HMPLRISSLP NGPDMQLYRH FTYGNLASFN VLDTRQYRDD QANNDGNKPP SDESRNPNRT 

       370        380        390        400        410        420 
LLGKEQEQWL FNNLGSSTAH WNVLAQQIFF AKWNFGTSAS PIYSMDSWDG YPAQRERVIN 

       430        440        450        460        470        480 
FIKSKNLNNV VVLTGDVHAS WASNLHVDFE KTSSKIFGAE FVGTSITSGG NGADKRADTD 

       490        500        510        520        530        540 
QILKENPHIQ FFNDYRGYVR CTVTPHQWKA DYRVMPFVTE PGAAISTRAS FVYQKDQTGL 

       550        560        570        580 
RKVSSTTIQG GVKQSDEVEE DRFFSHNKAH EKQMIKKRAK ITN 

« Hide

References

« Hide 'large scale' references
[1]"A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD."
Eder S., Shi L., Jensen K., Yamane K., Hulett F.M.
Microbiology 142:2041-2047(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 57-76.
Strain: 168 / JH642.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO C-TERMINUS.
[4]"Determination of a 21548 bp nucleotide sequence around the 24 degrees region of the Bacillus subtilis chromosome."
Ogawa K., Akagawa E., Nakamura K., Yamane K.
Microbiology 141:269-275(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-556.
Strain: 168.
[5]"Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis."
Yamane K., Maruo B.
J. Bacteriol. 134:100-107(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: 6060-BC6.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U49060 Genomic DNA. Translation: AAB47803.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB12056.2.
D30808 Genomic DNA. Translation: BAA06483.1. Frameshift.
PIRD69676.
RefSeqNP_388144.2. NC_000964.3.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2YEQX-ray1.93A/B57-583[»]
ProteinModelPortalP42251.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU02620.

Proteomic databases

PaxDbP42251.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB12056; CAB12056; BSU02620.
GeneID938391.
KEGGbsu:BSU02620.
PATRIC18972083. VBIBacSub10457_0270.

Organism-specific databases

GenoListBSU02620. [Micado]

Phylogenomic databases

eggNOGCOG3540.
HOGENOMHOG000240657.
KOK01113.
OrthoDBEOG6KT2N1.
PhylomeDBP42251.

Enzyme and pathway databases

BioCycBSUB:BSU02620-MONOMER.

Family and domain databases

Gene3D3.60.21.10. 1 hit.
InterProIPR018946. Alk_phosphatase_PhoD-like.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamPF09423. PhoD. 1 hit.
[Graphical view]
SUPFAMSSF56300. SSF56300. 1 hit.
ProtoNetSearch...

Entry information

Entry namePPBD_BACSU
AccessionPrimary (citable) accession number: P42251
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2009
Last modified: July 9, 2014
This is version 97 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList