P42251 (PPBD_BACSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline phosphatase D
Short name=APaseD
EC=3.1.3.1

Gene names

Name:	phoD
Synonyms:	ycbS
Ordered Locus Names:	BSU02620

Organism

Bacillus subtilis (strain 168) [Reference proteome] [HAMAP]

Taxonomic identifier

224308 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Bacillaceae › Bacillus › Bacillus subtilis group › Bacillus subtilis › Bacillus subtilis subsp. subtilis

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Induction	By phosphate starvation.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.
Sequence similarities	Belongs to the PhoD family.
Sequence caution	The sequence AAB47803.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577. The sequence BAA06483.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.

Ontologies

Keywords
Biological process	Stress response
Domain	Signal
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 56

56

Tat-type signal Ref.1

Chain

57 – 583

527

Alkaline phosphatase D

PRO_0000022088

Natural variations

Natural variant

59

1

N → K.

Secondary structure

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583

Helix Strand Turn

Details...

Beta strand

69 – 72

4

Beta strand

79 – 84

6

Helix

89 – 91

3

Beta strand

100 – 109

10

Beta strand

112 – 123

12

Helix

125 – 127

3

Beta strand

130 – 135

6

Beta strand

143 – 150

8

Beta strand

158 – 161

4

Beta strand

173 – 178

6

Helix

183 – 185

3

Helix

189 – 194

6

Beta strand

200 – 204

5

Beta strand

228 – 231

4

Helix

235 – 246

12

Helix

249 – 257

9

Beta strand

258 – 262

5

Turn

267 – 269

3

Helix

284 – 301

18

Helix

306 – 308

3

Beta strand

319 – 323

5

Turn

324 – 326

3

Beta strand

327 – 331

5

Beta strand

334 – 337

4

Helix

342 – 344

3

Beta strand

345 – 348

4

Helix

352 – 355

4

Helix

364 – 376

13

Beta strand

379 – 385

7

Beta strand

398 – 400

3

Helix

408 – 410

3

Helix

412 – 424

13

Beta strand

430 – 434

5

Beta strand

436 – 448

13

Beta strand

456 – 462

7

Beta strand

473 – 475

3

Helix

479 – 485

7

Beta strand

489 – 494

6

Beta strand

496 – 504

9

Beta strand

507 – 516

10

Beta strand

518 – 521

4

Beta strand

526 – 533

8

Beta strand

540 – 545

6

Helix

560 – 576

17

Sequences

	Sequence		Length	Mass (Da)
	P42251 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: 7734224CB2470319	FASTA	583	65,971
	10 20 30 40 50 60 MAYDSRFDEW VQKLKEESFQ NNTFDRRKFI QGAGKIAGLS LGLTIAQSVG AFEVNAAPNF 70 80 90 100 110 120 SSYPFTLGVA SGDPLSDSVV LWTRLAPDPL NGGGMPKQAV PVKWEVAKDE HFRKIVRKGT 130 140 150 160 170 180 EMAKPSLAHS VHVEADGLEP NKVYYYRFKT GHELSPVGKT KTLPAPGANV PQMTFAFASC 190 200 210 220 230 240 QQYEHGYYTA YKHMAKEKLD LVFHLGDYIY EYGPNEYVSK TGNVRTHNSA EIITLQDYRN 250 260 270 280 290 300 RHAQYRSDAN LKAAHAAFPW VVTWDDHEVE NNYANKIPEK GQSVEAFVLR RAAAYQAYYE 310 320 330 340 350 360 HMPLRISSLP NGPDMQLYRH FTYGNLASFN VLDTRQYRDD QANNDGNKPP SDESRNPNRT 370 380 390 400 410 420 LLGKEQEQWL FNNLGSSTAH WNVLAQQIFF AKWNFGTSAS PIYSMDSWDG YPAQRERVIN 430 440 450 460 470 480 FIKSKNLNNV VVLTGDVHAS WASNLHVDFE KTSSKIFGAE FVGTSITSGG NGADKRADTD 490 500 510 520 530 540 QILKENPHIQ FFNDYRGYVR CTVTPHQWKA DYRVMPFVTE PGAAISTRAS FVYQKDQTGL 550 560 570 580 RKVSSTTIQG GVKQSDEVEE DRFFSHNKAH EKQMIKKRAK ITN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD." Eder S., Shi L., Jensen K., Yamane K., Hulett F.M. Microbiology 142:2041-2047(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 57-76. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract] Cited for: SEQUENCE REVISION TO C-TERMINUS.
[4]	"Determination of a 21548 bp nucleotide sequence around the 24 degrees region of the Bacillus subtilis chromosome." Ogawa K., Akagawa E., Nakamura K., Yamane K. Microbiology 141:269-275(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-556. Strain: 168.
[5]	"Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis." Yamane K., Maruo B. J. Bacteriol. 134:100-107(1978) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: 6060-BC6.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U49060 Genomic DNA. Translation: AAB47803.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB12056.2.
D30808 Genomic DNA. Translation: BAA06483.1. Frameshift.

PIR

D69676.

RefSeq

NP_388144.2. NC_000964.3.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2YEQ	X-ray	1.93	A/B	57-583	[»]

ProteinModelPortal

P42251.

ModBase

Search...

Protein-protein interaction databases

STRING

224308.BSU02620.

Proteomic databases

PaxDb

P42251.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

CAB12056; CAB12056; BSU02620.

GeneID

938391.

KEGG

bsu:BSU02620.

PATRIC

18972083. VBIBacSub10457_0270.

Organism-specific databases

GenoList

BSU02620. [Micado]

Phylogenomic databases

eggNOG

COG3540.

HOGENOM

HOG000240657.

KO

K01113.

ProtClustDB

CLSK886627.

Enzyme and pathway databases

BioCyc

BSUB:BSU02620-MONOMER.

Family and domain databases

InterPro

IPR018946. Alk_phosphatase_PhoD-like.
[Graphical view]

Pfam

PF09423. PhoD. 1 hit.
[Graphical view]

PROSITE

PS51318. TAT. False negative.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

PPBD_BACSU

Accession

Primary (citable) accession number: P42251

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 16, 2009
Last modified:	May 1, 2013
This is version 90 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program