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P42251

- PPBD_BACSU

UniProt

P42251 - PPBD_BACSU

Protein

Alkaline phosphatase D

Gene

phoD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 98 (01 Oct 2014)
      Sequence version 3 (16 Jun 2009)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: UniProtKB-EC

    GO - Biological processi

    1. response to stress Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Stress response

    Enzyme and pathway databases

    BioCyciBSUB:BSU02620-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase D (EC:3.1.3.1)
    Short name:
    APaseD
    Gene namesi
    Name:phoD
    Synonyms:ycbS
    Ordered Locus Names:BSU02620
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU02620. [Micado]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 5656Tat-type signal1 PublicationAdd
    BLAST
    Chaini57 – 583527Alkaline phosphatase DPRO_0000022088Add
    BLAST

    Post-translational modificationi

    Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

    Proteomic databases

    PaxDbiP42251.

    Expressioni

    Inductioni

    By phosphate starvation.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU02620.

    Structurei

    Secondary structure

    1
    583
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi69 – 724
    Beta strandi79 – 846
    Helixi89 – 913
    Beta strandi100 – 10910
    Beta strandi112 – 12312
    Helixi125 – 1273
    Beta strandi130 – 1356
    Beta strandi143 – 1508
    Beta strandi158 – 1614
    Beta strandi173 – 1786
    Helixi183 – 1853
    Helixi189 – 1946
    Beta strandi200 – 2045
    Beta strandi228 – 2314
    Helixi235 – 24612
    Helixi249 – 2579
    Beta strandi258 – 2625
    Turni267 – 2693
    Helixi284 – 30118
    Helixi306 – 3083
    Beta strandi319 – 3235
    Turni324 – 3263
    Beta strandi327 – 3315
    Beta strandi334 – 3374
    Helixi342 – 3443
    Beta strandi345 – 3484
    Helixi352 – 3554
    Helixi364 – 37613
    Beta strandi379 – 3857
    Beta strandi398 – 4003
    Helixi408 – 4103
    Helixi412 – 42413
    Beta strandi430 – 4345
    Beta strandi436 – 44813
    Beta strandi456 – 4627
    Beta strandi473 – 4753
    Helixi479 – 4857
    Beta strandi489 – 4946
    Beta strandi496 – 5049
    Beta strandi507 – 51610
    Beta strandi518 – 5214
    Beta strandi526 – 5338
    Beta strandi540 – 5456
    Helixi560 – 57617

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2YEQX-ray1.93A/B57-583[»]
    ProteinModelPortaliP42251.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PhoD family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG3540.
    HOGENOMiHOG000240657.
    KOiK01113.
    OrthoDBiEOG6KT2N1.
    PhylomeDBiP42251.

    Family and domain databases

    Gene3Di3.60.21.10. 1 hit.
    InterProiIPR018946. Alk_phosphatase_PhoD-like.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view]
    PfamiPF09423. PhoD. 1 hit.
    [Graphical view]
    SUPFAMiSSF56300. SSF56300. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42251-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAYDSRFDEW VQKLKEESFQ NNTFDRRKFI QGAGKIAGLS LGLTIAQSVG    50
    AFEVNAAPNF SSYPFTLGVA SGDPLSDSVV LWTRLAPDPL NGGGMPKQAV 100
    PVKWEVAKDE HFRKIVRKGT EMAKPSLAHS VHVEADGLEP NKVYYYRFKT 150
    GHELSPVGKT KTLPAPGANV PQMTFAFASC QQYEHGYYTA YKHMAKEKLD 200
    LVFHLGDYIY EYGPNEYVSK TGNVRTHNSA EIITLQDYRN RHAQYRSDAN 250
    LKAAHAAFPW VVTWDDHEVE NNYANKIPEK GQSVEAFVLR RAAAYQAYYE 300
    HMPLRISSLP NGPDMQLYRH FTYGNLASFN VLDTRQYRDD QANNDGNKPP 350
    SDESRNPNRT LLGKEQEQWL FNNLGSSTAH WNVLAQQIFF AKWNFGTSAS 400
    PIYSMDSWDG YPAQRERVIN FIKSKNLNNV VVLTGDVHAS WASNLHVDFE 450
    KTSSKIFGAE FVGTSITSGG NGADKRADTD QILKENPHIQ FFNDYRGYVR 500
    CTVTPHQWKA DYRVMPFVTE PGAAISTRAS FVYQKDQTGL RKVSSTTIQG 550
    GVKQSDEVEE DRFFSHNKAH EKQMIKKRAK ITN 583
    Length:583
    Mass (Da):65,971
    Last modified:June 16, 2009 - v3
    Checksum:i7734224CB2470319
    GO

    Sequence cautioni

    The sequence AAB47803.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.
    The sequence BAA06483.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti59 – 591N → K.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49060 Genomic DNA. Translation: AAB47803.1. Frameshift.
    AL009126 Genomic DNA. Translation: CAB12056.2.
    D30808 Genomic DNA. Translation: BAA06483.1. Frameshift.
    PIRiD69676.
    RefSeqiNP_388144.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB12056; CAB12056; BSU02620.
    GeneIDi938391.
    KEGGibsu:BSU02620.
    PATRICi18972083. VBIBacSub10457_0270.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U49060 Genomic DNA. Translation: AAB47803.1 . Frameshift.
    AL009126 Genomic DNA. Translation: CAB12056.2 .
    D30808 Genomic DNA. Translation: BAA06483.1 . Frameshift.
    PIRi D69676.
    RefSeqi NP_388144.2. NC_000964.3.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2YEQ X-ray 1.93 A/B 57-583 [» ]
    ProteinModelPortali P42251.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU02620.

    Proteomic databases

    PaxDbi P42251.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB12056 ; CAB12056 ; BSU02620 .
    GeneIDi 938391.
    KEGGi bsu:BSU02620.
    PATRICi 18972083. VBIBacSub10457_0270.

    Organism-specific databases

    GenoListi BSU02620. [Micado ]

    Phylogenomic databases

    eggNOGi COG3540.
    HOGENOMi HOG000240657.
    KOi K01113.
    OrthoDBi EOG6KT2N1.
    PhylomeDBi P42251.

    Enzyme and pathway databases

    BioCyci BSUB:BSU02620-MONOMER.

    Family and domain databases

    Gene3Di 3.60.21.10. 1 hit.
    InterProi IPR018946. Alk_phosphatase_PhoD-like.
    IPR029052. Metallo-depent_PP-like.
    [Graphical view ]
    Pfami PF09423. PhoD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56300. SSF56300. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD."
      Eder S., Shi L., Jensen K., Yamane K., Hulett F.M.
      Microbiology 142:2041-2047(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 57-76.
      Strain: 168 / JH642.
    2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO C-TERMINUS.
    4. "Determination of a 21548 bp nucleotide sequence around the 24 degrees region of the Bacillus subtilis chromosome."
      Ogawa K., Akagawa E., Nakamura K., Yamane K.
      Microbiology 141:269-275(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-556.
      Strain: 168.
    5. "Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis."
      Yamane K., Maruo B.
      J. Bacteriol. 134:100-107(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: 6060-BC6.

    Entry informationi

    Entry nameiPPBD_BACSU
    AccessioniPrimary (citable) accession number: P42251
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: June 16, 2009
    Last modified: October 1, 2014
    This is version 98 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3