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P42251

- PPBD_BACSU

UniProt

P42251 - PPBD_BACSU

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Protein

Alkaline phosphatase D

Gene

phoD

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.

GO - Molecular functioni

  1. alkaline phosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. response to stress Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Stress response

Enzyme and pathway databases

BioCyciBSUB:BSU02620-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase D (EC:3.1.3.1)
Short name:
APaseD
Gene namesi
Name:phoD
Synonyms:ycbS
Ordered Locus Names:BSU02620
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU02620. [Micado]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 5656Tat-type signal1 PublicationAdd
BLAST
Chaini57 – 583527Alkaline phosphatase DPRO_0000022088Add
BLAST

Post-translational modificationi

Predicted to be exported by the Tat system. The position of the signal peptide cleavage has been experimentally proven.

Proteomic databases

PaxDbiP42251.

Expressioni

Inductioni

By phosphate starvation.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU02620.

Structurei

Secondary structure

1
583
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi69 – 724
Beta strandi79 – 846
Helixi89 – 913
Beta strandi100 – 10910
Beta strandi112 – 12312
Helixi125 – 1273
Beta strandi130 – 1356
Beta strandi143 – 1508
Beta strandi158 – 1614
Beta strandi173 – 1786
Helixi183 – 1853
Helixi189 – 1946
Beta strandi200 – 2045
Beta strandi228 – 2314
Helixi235 – 24612
Helixi249 – 2579
Beta strandi258 – 2625
Turni267 – 2693
Helixi284 – 30118
Helixi306 – 3083
Beta strandi319 – 3235
Turni324 – 3263
Beta strandi327 – 3315
Beta strandi334 – 3374
Helixi342 – 3443
Beta strandi345 – 3484
Helixi352 – 3554
Helixi364 – 37613
Beta strandi379 – 3857
Beta strandi398 – 4003
Helixi408 – 4103
Helixi412 – 42413
Beta strandi430 – 4345
Beta strandi436 – 44813
Beta strandi456 – 4627
Beta strandi473 – 4753
Helixi479 – 4857
Beta strandi489 – 4946
Beta strandi496 – 5049
Beta strandi507 – 51610
Beta strandi518 – 5214
Beta strandi526 – 5338
Beta strandi540 – 5456
Helixi560 – 57617

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2YEQX-ray1.93A/B57-583[»]
ProteinModelPortaliP42251.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PhoD family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG3540.
HOGENOMiHOG000240657.
InParanoidiP42251.
KOiK01113.
OrthoDBiEOG6KT2N1.
PhylomeDBiP42251.

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
InterProiIPR018946. Alk_phosphatase_PhoD-like.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PfamiPF09423. PhoD. 1 hit.
[Graphical view]
SUPFAMiSSF56300. SSF56300. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42251-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAYDSRFDEW VQKLKEESFQ NNTFDRRKFI QGAGKIAGLS LGLTIAQSVG
60 70 80 90 100
AFEVNAAPNF SSYPFTLGVA SGDPLSDSVV LWTRLAPDPL NGGGMPKQAV
110 120 130 140 150
PVKWEVAKDE HFRKIVRKGT EMAKPSLAHS VHVEADGLEP NKVYYYRFKT
160 170 180 190 200
GHELSPVGKT KTLPAPGANV PQMTFAFASC QQYEHGYYTA YKHMAKEKLD
210 220 230 240 250
LVFHLGDYIY EYGPNEYVSK TGNVRTHNSA EIITLQDYRN RHAQYRSDAN
260 270 280 290 300
LKAAHAAFPW VVTWDDHEVE NNYANKIPEK GQSVEAFVLR RAAAYQAYYE
310 320 330 340 350
HMPLRISSLP NGPDMQLYRH FTYGNLASFN VLDTRQYRDD QANNDGNKPP
360 370 380 390 400
SDESRNPNRT LLGKEQEQWL FNNLGSSTAH WNVLAQQIFF AKWNFGTSAS
410 420 430 440 450
PIYSMDSWDG YPAQRERVIN FIKSKNLNNV VVLTGDVHAS WASNLHVDFE
460 470 480 490 500
KTSSKIFGAE FVGTSITSGG NGADKRADTD QILKENPHIQ FFNDYRGYVR
510 520 530 540 550
CTVTPHQWKA DYRVMPFVTE PGAAISTRAS FVYQKDQTGL RKVSSTTIQG
560 570 580
GVKQSDEVEE DRFFSHNKAH EKQMIKKRAK ITN
Length:583
Mass (Da):65,971
Last modified:June 16, 2009 - v3
Checksum:i7734224CB2470319
GO

Sequence cautioni

The sequence AAB47803.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.
The sequence BAA06483.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti59 – 591N → K.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49060 Genomic DNA. Translation: AAB47803.1. Frameshift.
AL009126 Genomic DNA. Translation: CAB12056.2.
D30808 Genomic DNA. Translation: BAA06483.1. Frameshift.
PIRiD69676.
RefSeqiNP_388144.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB12056; CAB12056; BSU02620.
GeneIDi938391.
KEGGibsu:BSU02620.
PATRICi18972083. VBIBacSub10457_0270.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U49060 Genomic DNA. Translation: AAB47803.1 . Frameshift.
AL009126 Genomic DNA. Translation: CAB12056.2 .
D30808 Genomic DNA. Translation: BAA06483.1 . Frameshift.
PIRi D69676.
RefSeqi NP_388144.2. NC_000964.3.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2YEQ X-ray 1.93 A/B 57-583 [» ]
ProteinModelPortali P42251.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU02620.

Proteomic databases

PaxDbi P42251.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB12056 ; CAB12056 ; BSU02620 .
GeneIDi 938391.
KEGGi bsu:BSU02620.
PATRICi 18972083. VBIBacSub10457_0270.

Organism-specific databases

GenoListi BSU02620. [Micado ]

Phylogenomic databases

eggNOGi COG3540.
HOGENOMi HOG000240657.
InParanoidi P42251.
KOi K01113.
OrthoDBi EOG6KT2N1.
PhylomeDBi P42251.

Enzyme and pathway databases

BioCyci BSUB:BSU02620-MONOMER.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
InterProi IPR018946. Alk_phosphatase_PhoD-like.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
Pfami PF09423. PhoD. 1 hit.
[Graphical view ]
SUPFAMi SSF56300. SSF56300. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD."
    Eder S., Shi L., Jensen K., Yamane K., Hulett F.M.
    Microbiology 142:2041-2047(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 57-76.
    Strain: 168 / JH642.
  2. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  3. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO C-TERMINUS.
  4. "Determination of a 21548 bp nucleotide sequence around the 24 degrees region of the Bacillus subtilis chromosome."
    Ogawa K., Akagawa E., Nakamura K., Yamane K.
    Microbiology 141:269-275(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-556.
    Strain: 168.
  5. "Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis."
    Yamane K., Maruo B.
    J. Bacteriol. 134:100-107(1978) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: 6060-BC6.

Entry informationi

Entry nameiPPBD_BACSU
AccessioniPrimary (citable) accession number: P42251
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: June 16, 2009
Last modified: October 29, 2014
This is version 99 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3