Reviewed, UniProtKB/Swiss-Prot P42251 (PPBD_BACSU)

Names and origin

Protein names

Recommended name:
    Alkaline phosphatase D
      Short name=APaseD
    EC=3.1.3.1

Gene names

Name:	phoD
Synonyms:	ycbS
Ordered Locus Names:	BSU02620

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	583 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Induction	By phosphate starvation.
Sequence caution	The sequence AAB47803.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577. The sequence BAA06483.1 differs from that shown. Reason: Frameshift at positions 508, 565 and 577.

Ontologies

Keywords
Biological process	Stress response
Domain	Signal
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alkaline phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 56	56	Ref.1
	Chain	57 – 583	527	Alkaline phosphatase D		PRO_0000022088
Natural variations
	Natural variant	59	1	N → K

Sequences

	Sequence		Length	Mass (Da)
	P42251-1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: 7734224CB2470319	FASTA	583	65,971
	10 20 30 40 50 60 MAYDSRFDEW VQKLKEESFQ NNTFDRRKFI QGAGKIAGLS LGLTIAQSVG AFEVNAAPNF 70 80 90 100 110 120 SSYPFTLGVA SGDPLSDSVV LWTRLAPDPL NGGGMPKQAV PVKWEVAKDE HFRKIVRKGT 130 140 150 160 170 180 EMAKPSLAHS VHVEADGLEP NKVYYYRFKT GHELSPVGKT KTLPAPGANV PQMTFAFASC 190 200 210 220 230 240 QQYEHGYYTA YKHMAKEKLD LVFHLGDYIY EYGPNEYVSK TGNVRTHNSA EIITLQDYRN 250 260 270 280 290 300 RHAQYRSDAN LKAAHAAFPW VVTWDDHEVE NNYANKIPEK GQSVEAFVLR RAAAYQAYYE 310 320 330 340 350 360 HMPLRISSLP NGPDMQLYRH FTYGNLASFN VLDTRQYRDD QANNDGNKPP SDESRNPNRT 370 380 390 400 410 420 LLGKEQEQWL FNNLGSSTAH WNVLAQQIFF AKWNFGTSAS PIYSMDSWDG YPAQRERVIN 430 440 450 460 470 480 FIKSKNLNNV VVLTGDVHAS WASNLHVDFE KTSSKIFGAE FVGTSITSGG NGADKRADTD 490 500 510 520 530 540 QILKENPHIQ FFNDYRGYVR CTVTPHQWKA DYRVMPFVTE PGAAISTRAS FVYQKDQTGL 550 560 570 580 RKVSSTTIQG GVKQSDEVEE DRFFSHNKAH EKQMIKKRAK ITN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A Bacillus subtilis secreted phosphodiesterase/alkaline phosphatase is the product of a Pho regulon gene, phoD." Eder S., Shi L., Jensen K., Yamane K., Hulett F.M. Microbiology 142:2041-2047(1996) [PubMed: 8760916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 57-76. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO C-TERMINUS.
[4]	"Determination of a 21548 bp nucleotide sequence around the 24 degrees region of the Bacillus subtilis chromosome." Ogawa K., Akagawa E., Nakamura K., Yamane K. Microbiology 141:269-275(1995) [PubMed: 7704254] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 95-556. Strain: 168.
[5]	"Purification and characterization of extracellular soluble and membrane-bound insoluble alkaline phosphatases possessing phosphodiesterase activities in Bacillus subtilis." Yamane K., Maruo B. J. Bacteriol. 134:100-107(1978) [PubMed: 25878] [Abstract] Cited for: CHARACTERIZATION. Strain: 6060-BC6.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U49060 Genomic DNA. Translation: AAB47803.1. Frameshift. AL009126 Genomic DNA. Translation: CAB12056.2. D30808 Genomic DNA. Translation: BAA06483.1. Frameshift.
PIR	D69676.
RefSeq	NP_388144.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	938391.
GenomeReviews	Gene locus BSU02620 in contig AL009126_GR.
KEGG	bsu:BSU02620.
NMPDR	fig|224308.1.peg.263.
Organism-specific databases
SubtiList	BG11174. phoD. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P42251.
OMA	YRHLAAE.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU0263-MON.
BRENDA	3.1.3.1. 150.
Family and domain databases
InterPro	IPR018946. Alk_phosphatase_PhoD-like. IPR006311. TAT_signal. [Graphical view]
Pfam	PF09423. PhoD. 1 hit. [Graphical view]
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PPBD_BACSU

Accession

Primary (citable) accession number: P42251

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 16, 2009
Last modified:	November 3, 2009
This is version 65 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)