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Protein

Signal transducer and activator of transcription 5B

Gene

Stat5b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. Binds to the GAS element and activates PRL-induced transcription. Positively regulates hematopoietic/erythroid differentiation.1 Publication

GO - Molecular functioni

  • chromatin binding Source: UniProtKB
  • DNA binding Source: MGI
  • glucocorticoid receptor binding Source: MGI
  • protein dimerization activity Source: UniProtKB
  • RNA polymerase II proximal promoter sequence-specific DNA binding Source: MGI
  • signal transducer activity Source: MGI
  • transcriptional activator activity, RNA polymerase II proximal promoter sequence-specific DNA binding Source: NTNU_SB

GO - Biological processi

  • 2-oxoglutarate metabolic process Source: BHF-UCL
  • allantoin metabolic process Source: BHF-UCL
  • cellular response to epidermal growth factor stimulus Source: UniProtKB
  • cellular response to growth factor stimulus Source: UniProtKB
  • cellular response to hormone stimulus Source: MGI
  • citrate metabolic process Source: BHF-UCL
  • creatine metabolic process Source: BHF-UCL
  • creatinine metabolic process Source: BHF-UCL
  • cytokine-mediated signaling pathway Source: MGI
  • development of secondary female sexual characteristics Source: MGI
  • development of secondary male sexual characteristics Source: MGI
  • fatty acid metabolic process Source: BHF-UCL
  • female pregnancy Source: MGI
  • isoleucine metabolic process Source: BHF-UCL
  • JAK-STAT cascade Source: MGI
  • JAK-STAT cascade involved in growth hormone signaling pathway Source: BHF-UCL
  • lactation Source: MGI
  • lipid storage Source: MGI
  • luteinization Source: MGI
  • mast cell migration Source: MGI
  • natural killer cell differentiation Source: MGI
  • negative regulation of apoptotic process Source: MGI
  • negative regulation of erythrocyte differentiation Source: MGI
  • oxaloacetate metabolic process Source: BHF-UCL
  • Peyer's patch development Source: MGI
  • positive regulation of activated T cell proliferation Source: MGI
  • positive regulation of B cell differentiation Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of erythrocyte differentiation Source: UniProtKB
  • positive regulation of gamma-delta T cell differentiation Source: MGI
  • positive regulation of inflammatory response Source: MGI
  • positive regulation of interleukin-2 biosynthetic process Source: MGI
  • positive regulation of lymphocyte differentiation Source: MGI
  • positive regulation of mitotic cell cycle Source: MGI
  • positive regulation of multicellular organism growth Source: MGI
  • positive regulation of natural killer cell differentiation Source: MGI
  • positive regulation of natural killer cell mediated cytotoxicity Source: MGI
  • positive regulation of natural killer cell proliferation Source: MGI
  • positive regulation of transcription by RNA polymerase II Source: UniProtKB
  • progesterone metabolic process Source: MGI
  • regulation of cell adhesion Source: MGI
  • regulation of epithelial cell differentiation Source: MGI
  • regulation of multicellular organism growth Source: BHF-UCL
  • regulation of steroid metabolic process Source: MGI
  • regulation of transcription by RNA polymerase II Source: MGI
  • response to estradiol Source: MGI
  • response to interleukin-15 Source: MGI
  • response to interleukin-2 Source: MGI
  • response to interleukin-4 Source: MGI
  • sex differentiation Source: MGI
  • succinate metabolic process Source: BHF-UCL
  • taurine metabolic process Source: BHF-UCL
  • T cell differentiation in thymus Source: MGI
  • T cell homeostasis Source: MGI
  • valine metabolic process Source: BHF-UCL

Keywordsi

Molecular functionActivator, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-1266695 Interleukin-7 signaling
R-MMU-1433557 Signaling by SCF-KIT
R-MMU-186763 Downstream signal transduction
R-MMU-2586552 Signaling by Leptin
R-MMU-512988 Interleukin-3, 5 and GM-CSF signaling
R-MMU-8854691 Interleukin-20 family signaling
R-MMU-8983432 Interleukin-15 signaling
R-MMU-9020558 Interleukin-2 signaling
R-MMU-9020958 Interleukin-21 signaling
R-MMU-982772 Growth hormone receptor signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducer and activator of transcription 5B
Gene namesi
Name:Stat5b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:103035 Stat5b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi684T → A: Fails to interact with INSR. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001824301 – 786Signal transducer and activator of transcription 5BAdd BLAST786

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei90PhosphotyrosineBy similarity1
Modified residuei128PhosphoserineBy similarity1
Modified residuei699PhosphotyrosineCombined sources1

Post-translational modificationi

Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 (By similarity). Phosphorylation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity (By similarity).By similarity3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP42232
MaxQBiP42232
PaxDbiP42232
PeptideAtlasiP42232
PRIDEiP42232

PTM databases

iPTMnetiP42232
PhosphoSitePlusiP42232
SwissPalmiP42232

Expressioni

Tissue specificityi

In the virgin, found in most tissues. Particularly abundant in muscle tissue of virgin and lactating females, and of males.1 Publication

Developmental stagei

Detected both in virgin mouse and after mammary gland involution. The level of STAT5A increases constantly during pregnancy, but decreases during lactation.1 Publication

Gene expression databases

BgeeiENSMUSG00000020919
GenevisibleiP42232 MM

Interactioni

Subunit structurei

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1 (PubMed:9528750). Interacts with NCOA1 (By similarity). Interacts with SOCS7 (By similarity). Interacts (via SH2 domain) with INSR (PubMed:9428692). Interacts with CPEB3; this inhibits STAT5B-mediated transcriptional activation (PubMed:20639532).By similarity3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • glucocorticoid receptor binding Source: MGI
  • protein dimerization activity Source: UniProtKB

Protein-protein interaction databases

BioGridi203526, 6 interactors
DIPiDIP-898N
IntActiP42232, 4 interactors
MINTiP42232
STRINGi10090.ENSMUSP00000004143

Structurei

3D structure databases

ProteinModelPortaliP42232
SMRiP42232
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini589 – 686SH2PROSITE-ProRule annotationAdd BLAST98

Sequence similaritiesi

Belongs to the transcription factor STAT family.Curated

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiKOG3667 Eukaryota
ENOG410XPN8 LUCA
GeneTreeiENSGT00760000119236
HOVERGENiHBG107486
InParanoidiP42232
KOiK11224
OMAiYHQTTGT
OrthoDBiEOG091G03O3
PhylomeDBiP42232
TreeFamiTF318648

Family and domain databases

CDDicd10420 SH2_STAT5b, 1 hit
Gene3Di1.10.532.10, 1 hit
2.60.40.630, 1 hit
3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR008967 p53-like_TF_DNA-bd
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR001217 STAT
IPR035858 STAT5a/5b
IPR035886 STAT5b_SH2
IPR036535 STAT_N_sf
IPR013800 STAT_TF_alpha
IPR015988 STAT_TF_coiled-coil
IPR013801 STAT_TF_DNA-bd
IPR012345 STAT_TF_DNA-bd_N
IPR013799 STAT_TF_prot_interaction
PANTHERiPTHR11801 PTHR11801, 1 hit
PTHR11801:SF39 PTHR11801:SF39, 1 hit
PfamiView protein in Pfam
PF00017 SH2, 1 hit
PF01017 STAT_alpha, 1 hit
PF02864 STAT_bind, 1 hit
PF02865 STAT_int, 1 hit
SMARTiView protein in SMART
SM00252 SH2, 1 hit
SM00964 STAT_int, 1 hit
SUPFAMiSSF47655 SSF47655, 1 hit
SSF48092 SSF48092, 1 hit
SSF49417 SSF49417, 1 hit
SSF55550 SSF55550, 1 hit
PROSITEiView protein in PROSITE
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

P42232-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAMWIQAQQL QGDALHQMQA LYGQHFPIEV RHYLSQWIES QAWDSIDLDN
60 70 80 90 100
PQENIKATQL LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQSTYDR
110 120 130 140 150
CPMELVRCIR HILYNEQRLV REANNGSSPA GSLADAMSQK HLQINQTFEE
160 170 180 190 200
LRLITQDTEN ELKKLQQTQE YFIIQYQESL RIQAQFAQLG QLNPQERMSR
210 220 230 240 250
ETALQQKQVS LETWLQREAQ TLQQYRVELA EKHQKTLQLL RKQQTIILDD
260 270 280 290 300
ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC
310 320 330 340 350
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA
360 370 380 390 400
TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN DYSGEILNNC
410 420 430 440 450
CVMEYHQATG TLSAHFRNMS LKRIKRSDRR GAESVTEEKF TILFDSQFSV
460 470 480 490 500
GGNELVFQVK TLSLPVVVIV HGSQDNNATA TVLWDNAFAE PGRVPFAVPD
510 520 530 540 550
KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNI SSNHLEDYNS
560 570 580 590 600
MSVSWSQFNR ENLPGRNYTF WQWFDGVMEV LKKHLKPHWN DGAILGFVNK
610 620 630 640 650
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSQERMF WNLMPFTTRD
660 670 680 690 700
FSIRSLADRL GDLNYLIYVF PDRPKDEVYS KYYTPVPCEP ATAKAADGYV
710 720 730 740 750
KPQIKQVVPE FANASTDAGS GATYMDQAPS PVVCPQAHYN MYPPNPDSVL
760 770 780
DTDGDFDLED TMDVARRVEE LLGRPMDSQW IPHAQS
Length:786
Mass (Da):90,002
Last modified:November 1, 1995 - v1
Checksum:iA8FE76405E41B2EF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210S → P in CAB51862 (PubMed:10835485).Curated1
Sequence conflicti433E → G in AAC52282 (PubMed:7568026).Curated1
Sequence conflicti433E → G in AAL05590 (PubMed:14701862).Curated1
Sequence conflicti433E → G in AAK97791 (PubMed:14701862).Curated1
Sequence conflicti433E → G in AAK97792 (PubMed:14701862).Curated1
Sequence conflicti433E → G in AAK97793 (PubMed:14701862).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti327L → M in strain: NOD; reduces DNA-binding affinity. 1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z48539 mRNA Translation: CAA88420.1
U21110 mRNA Translation: AAC52282.1
AF234171 Genomic DNA Translation: AAF62911.2
AY040231 mRNA Translation: AAK74074.1
AY042906 mRNA Translation: AAL05590.1
AY044901 mRNA Translation: AAK97791.1
AY044902 mRNA Translation: AAK97792.1
AY044903 mRNA Translation: AAK97793.1
AK150098 mRNA Translation: BAE29305.1
AK154014 mRNA Translation: BAE32317.1
AK154664 mRNA Translation: BAE32752.1
AL591466 Genomic DNA Translation: CAM19465.1
BC024319 mRNA Translation: AAH24319.1
AJ237939 Genomic DNA Translation: CAB51862.1
CCDSiCCDS25438.1
PIRiI49274
RefSeqiNP_001107035.1, NM_001113563.1
NP_035619.3, NM_011489.3
XP_017169892.1, XM_017314403.1
UniGeneiMm.34064

Genome annotation databases

EnsembliENSMUST00000004143; ENSMUSP00000004143; ENSMUSG00000020919
ENSMUST00000107358; ENSMUSP00000102981; ENSMUSG00000020919
GeneIDi20851
KEGGimmu:20851
UCSCiuc007lmi.2 mouse

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiSTA5B_MOUSE
AccessioniPrimary (citable) accession number: P42232
Secondary accession number(s): A2A5D5
, Q541Q5, Q60804, Q8K3Q1, Q9JKM1, Q9R0X8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 187 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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