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P42231 (STA5A_SHEEP) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal transducer and activator of transcription 5A
Alternative name(s):
Mammary gland factor
Gene names
Name:STAT5A
Synonyms:MGF, STAT5
OrganismOvis aries (Sheep) [Complete proteome]
Taxonomic identifier9940 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis

Protein attributes

Sequence length794 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

Subunit structure

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4 By similarity.

Subcellular location

Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Tissue specificity

Found in mammary gland and, in lesser extent, in ovary, thymus, spleen, kidney, lung, muscle and adrenal gland.

Induction

By prolactin.

Post-translational modification

Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway By similarity.

ISGylated By similarity.

Sequence similarities

Belongs to the transcription factor STAT family.

Contains 1 SH2 domain.

Ontologies

Keywords
   Biological processLactation
Transcription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainSH2 domain
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
Ubl conjugation
   Technical termComplete proteome
Direct protein sequencing
Gene Ontology (GO)
   Biological_processJAK-STAT cascade

Inferred from sequence or structural similarity. Source: AgBase

T cell homeostasis

Inferred from sequence or structural similarity. Source: AgBase

cytokine-mediated signaling pathway

Inferred from sequence or structural similarity. Source: AgBase

development of secondary female sexual characteristics

Inferred from sequence or structural similarity. Source: AgBase

female pregnancy

Inferred from sequence or structural similarity. Source: AgBase

lactation

Inferred from sequence or structural similarity. Source: AgBase

lipid storage

Inferred from sequence or structural similarity. Source: AgBase

luteinization

Inferred from sequence or structural similarity. Source: AgBase

mammary gland development

Inferred from sequence or structural similarity. Source: AgBase

natural killer cell differentiation

Inferred from sequence or structural similarity. Source: AgBase

negative regulation of apoptotic process

Inferred from sequence or structural similarity. Source: AgBase

negative regulation of erythrocyte differentiation

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of B cell differentiation

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of activated T cell proliferation

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of cell proliferation

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of inflammatory response

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of interleukin-2 biosynthetic process

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of mitotic cell cycle

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of multicellular organism growth

Inferred from sequence or structural similarity. Source: AgBase

positive regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: AgBase

prolactin signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of cell adhesion

Inferred from sequence or structural similarity. Source: AgBase

regulation of epithelial cell differentiation

Inferred from sequence or structural similarity. Source: AgBase

regulation of steroid metabolic process

Inferred from sequence or structural similarity. Source: AgBase

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from sequence or structural similarity. Source: AgBase

nucleus

Inferred from sequence or structural similarity. Source: AgBase

   Molecular_functionDNA binding

Inferred from sequence or structural similarity. Source: AgBase

calcium ion binding

Inferred from electronic annotation. Source: InterPro

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

signal transducer activity

Inferred from sequence or structural similarity. Source: AgBase

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 794794Signal transducer and activator of transcription 5A
PRO_0000182427

Regions

Domain589 – 68698SH2

Amino acid modifications

Modified residue1291Phosphoserine By similarity
Modified residue6941Phosphotyrosine; by JAK2 By similarity

Sequences

Sequence LengthMass (Da)Tools
P42231 [UniParc].

Last modified February 1, 1996. Version 2.
Checksum: E69A4EC826AF60BE

FASTA79490,917
        10         20         30         40         50         60 
MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN PQDRAQVTQL 

        70         80         90        100        110        120 
LEGLVQELQK KAEHQVGEDG FLLKIKLGHY VHVSSRTRTT AAPWSWLRCI RHILYNEQRL 

       130        140        150        160        170        180 
VREATNGNSS AGILVDAMSQ KHLQINQTFE ELRLVTQDTE NELKKLQQTQ EYFIIQYQES 

       190        200        210        220        230        240 
LRIQAQFAQL AQLNPQERLS RETALQQKQV SLEAWLQREA QTLQQYRVEL AEKHQKTLQL 

       250        260        270        280        290        300 
LRKQQTIILD DELIQWKRRH DWRGMEAPPR SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 

       310        320        330        340        350        360 
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA TVRLLVGGKL 

       370        380        390        400        410        420 
NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC CVMEYHQRTG TLSAHFRNMS 

       430        440        450        460        470        480 
LKRIKRADRR GAESVTEEKF TVLFESQFSV GSNELVFQVK TLSLPVVVIV HGSQDHNATA 

       490        500        510        520        530        540 
TVLWDNAFAE PGRVPFAVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL LFLAQKLFNN 

       550        560        570        580        590        600 
SSSHLEDYNG MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 

       610        620        630        640        650        660 
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRE GSIRSLADRL 

       670        680        690        700        710        720 
GDLNYLIYVF PDRPKDEVFS KYYTPVLAKA VDGYVKPQIK QVVPEFVSAS ADSAGSSATY 

       730        740        750        760        770        780 
MDQAPSPAVC PQPHYNMYPQ NPDPVLDQDG EFDLDETMDV ARHVEELLRR PNGQSGPLSP 

       790 
PPAGLFTPAR GSLS 

« Hide

References

[1]"Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response."
Wakao H., Gouilleux F., Groner B.
EMBO J. 13:2182-2191(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Mammary gland.
[2]Erratum
Wakao H., Gouilleux F., Groner B.
EMBO J. 14:854-855(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION.
[3]"Interleukin-3 signals through multiple isoforms of Stat5."
Azam M., Erdjument-Bromage H., Kreider B.L., Xia M., Quelle F., Basu R., Saris C., Tempst P., Ihle J.N., Schindler C.
EMBO J. 14:1402-1411(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: PARTIAL PROTEIN SEQUENCE.
Tissue: Mammary gland.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X78428 mRNA. Translation: CAA55191.1.
PIRS55527.
RefSeqNP_001009402.1. NM_001009402.1.
UniGeneOar.13291.

3D structure databases

ProteinModelPortalP42231.
SMRP42231. Positions 139-690.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID443419.

Organism-specific databases

CTD6776.

Phylogenomic databases

HOVERGENHBG107486.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
PANTHERPTHR11801. PTHR11801. 1 hit.
PfamPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
SMARTSM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view]
SUPFAMSSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSTA5A_SHEEP
AccessionPrimary (citable) accession number: P42231
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families