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P42231

- STA5A_SHEEP

UniProt

P42231 - STA5A_SHEEP

Protein

Signal transducer and activator of transcription 5A

Gene

STAT5A

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

    GO - Molecular functioni

    1. calcium ion binding Source: InterPro
    2. DNA binding Source: AgBase
    3. sequence-specific DNA binding transcription factor activity Source: InterPro
    4. signal transducer activity Source: AgBase

    GO - Biological processi

    1. cytokine-mediated signaling pathway Source: AgBase
    2. development of secondary female sexual characteristics Source: AgBase
    3. female pregnancy Source: AgBase
    4. JAK-STAT cascade Source: AgBase
    5. lactation Source: AgBase
    6. lipid storage Source: AgBase
    7. luteinization Source: AgBase
    8. mammary gland development Source: AgBase
    9. natural killer cell differentiation Source: AgBase
    10. negative regulation of apoptotic process Source: AgBase
    11. negative regulation of erythrocyte differentiation Source: AgBase
    12. positive regulation of activated T cell proliferation Source: AgBase
    13. positive regulation of B cell differentiation Source: AgBase
    14. positive regulation of cell proliferation Source: AgBase
    15. positive regulation of inflammatory response Source: AgBase
    16. positive regulation of interleukin-2 biosynthetic process Source: AgBase
    17. positive regulation of mitotic cell cycle Source: AgBase
    18. positive regulation of multicellular organism growth Source: AgBase
    19. positive regulation of transcription from RNA polymerase II promoter Source: AgBase
    20. prolactin signaling pathway Source: UniProtKB
    21. regulation of cell adhesion Source: AgBase
    22. regulation of epithelial cell differentiation Source: AgBase
    23. regulation of steroid metabolic process Source: AgBase
    24. T cell homeostasis Source: AgBase
    25. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Lactation, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Signal transducer and activator of transcription 5A
    Alternative name(s):
    Mammary gland factor
    Gene namesi
    Name:STAT5A
    Synonyms:MGF, STAT5
    OrganismiOvis aries (Sheep)
    Taxonomic identifieri9940 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
    ProteomesiUP000002356: Unplaced

    Subcellular locationi

    Cytoplasm. Nucleus
    Note: Translocated into the nucleus in response to phosphorylation.

    GO - Cellular componenti

    1. cytoplasm Source: AgBase
    2. nucleus Source: AgBase

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 794794Signal transducer and activator of transcription 5APRO_0000182427Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei129 – 1291PhosphoserineBy similarity
    Modified residuei694 – 6941Phosphotyrosine; by JAK2By similarity

    Post-translational modificationi

    Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway By similarity.By similarity
    ISGylated.By similarity

    Keywords - PTMi

    Phosphoprotein, Ubl conjugation

    Expressioni

    Tissue specificityi

    Found in mammary gland and, in lesser extent, in ovary, thymus, spleen, kidney, lung, muscle and adrenal gland.

    Inductioni

    By prolactin.

    Interactioni

    Subunit structurei

    Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4 By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliP42231.
    SMRiP42231. Positions 139-690.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini589 – 68698SH2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the transcription factor STAT family.Curated
    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    SH2 domain

    Phylogenomic databases

    HOVERGENiHBG107486.

    Family and domain databases

    Gene3Di1.10.238.10. 1 hit.
    1.10.532.10. 1 hit.
    1.20.1050.20. 1 hit.
    2.60.40.630. 1 hit.
    3.30.505.10. 1 hit.
    InterProiIPR011992. EF-hand-dom_pair.
    IPR008967. p53-like_TF_DNA-bd.
    IPR000980. SH2.
    IPR001217. STAT.
    IPR013800. STAT_TF_alpha.
    IPR015988. STAT_TF_coiled-coil.
    IPR013801. STAT_TF_DNA-bd.
    IPR012345. STAT_TF_DNA-bd_sub.
    IPR013799. STAT_TF_prot_interaction.
    [Graphical view]
    PANTHERiPTHR11801. PTHR11801. 1 hit.
    PfamiPF00017. SH2. 1 hit.
    PF01017. STAT_alpha. 1 hit.
    PF02864. STAT_bind. 1 hit.
    PF02865. STAT_int. 1 hit.
    [Graphical view]
    SMARTiSM00252. SH2. 1 hit.
    SM00964. STAT_int. 1 hit.
    [Graphical view]
    SUPFAMiSSF47655. SSF47655. 1 hit.
    SSF48092. SSF48092. 1 hit.
    SSF49417. SSF49417. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEiPS50001. SH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42231-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN    50
    PQDRAQVTQL LEGLVQELQK KAEHQVGEDG FLLKIKLGHY VHVSSRTRTT 100
    AAPWSWLRCI RHILYNEQRL VREATNGNSS AGILVDAMSQ KHLQINQTFE 150
    ELRLVTQDTE NELKKLQQTQ EYFIIQYQES LRIQAQFAQL AQLNPQERLS 200
    RETALQQKQV SLEAWLQREA QTLQQYRVEL AEKHQKTLQL LRKQQTIILD 250
    DELIQWKRRH DWRGMEAPPR SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 300
    QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA 350
    TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC 400
    CVMEYHQRTG TLSAHFRNMS LKRIKRADRR GAESVTEEKF TVLFESQFSV 450
    GSNELVFQVK TLSLPVVVIV HGSQDHNATA TVLWDNAFAE PGRVPFAVPD 500
    KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL LFLAQKLFNN SSSHLEDYNG 550
    MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 600
    QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRE 650
    GSIRSLADRL GDLNYLIYVF PDRPKDEVFS KYYTPVLAKA VDGYVKPQIK 700
    QVVPEFVSAS ADSAGSSATY MDQAPSPAVC PQPHYNMYPQ NPDPVLDQDG 750
    EFDLDETMDV ARHVEELLRR PNGQSGPLSP PPAGLFTPAR GSLS 794
    Length:794
    Mass (Da):90,917
    Last modified:February 1, 1996 - v2
    Checksum:iE69A4EC826AF60BE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78428 mRNA. Translation: CAA55191.1.
    PIRiS55527.
    RefSeqiNP_001009402.1. NM_001009402.1.
    UniGeneiOar.13291.

    Genome annotation databases

    GeneIDi443419.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X78428 mRNA. Translation: CAA55191.1 .
    PIRi S55527.
    RefSeqi NP_001009402.1. NM_001009402.1.
    UniGenei Oar.13291.

    3D structure databases

    ProteinModelPortali P42231.
    SMRi P42231. Positions 139-690.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 443419.

    Organism-specific databases

    CTDi 6776.

    Phylogenomic databases

    HOVERGENi HBG107486.

    Family and domain databases

    Gene3Di 1.10.238.10. 1 hit.
    1.10.532.10. 1 hit.
    1.20.1050.20. 1 hit.
    2.60.40.630. 1 hit.
    3.30.505.10. 1 hit.
    InterProi IPR011992. EF-hand-dom_pair.
    IPR008967. p53-like_TF_DNA-bd.
    IPR000980. SH2.
    IPR001217. STAT.
    IPR013800. STAT_TF_alpha.
    IPR015988. STAT_TF_coiled-coil.
    IPR013801. STAT_TF_DNA-bd.
    IPR012345. STAT_TF_DNA-bd_sub.
    IPR013799. STAT_TF_prot_interaction.
    [Graphical view ]
    PANTHERi PTHR11801. PTHR11801. 1 hit.
    Pfami PF00017. SH2. 1 hit.
    PF01017. STAT_alpha. 1 hit.
    PF02864. STAT_bind. 1 hit.
    PF02865. STAT_int. 1 hit.
    [Graphical view ]
    SMARTi SM00252. SH2. 1 hit.
    SM00964. STAT_int. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47655. SSF47655. 1 hit.
    SSF48092. SSF48092. 1 hit.
    SSF49417. SSF49417. 1 hit.
    SSF55550. SSF55550. 1 hit.
    PROSITEi PS50001. SH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response."
      Wakao H., Gouilleux F., Groner B.
      EMBO J. 13:2182-2191(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Mammary gland.
    2. Erratum
      Wakao H., Gouilleux F., Groner B.
      EMBO J. 14:854-855(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION.
    3. "Interleukin-3 signals through multiple isoforms of Stat5."
      Azam M., Erdjument-Bromage H., Kreider B.L., Xia M., Quelle F., Basu R., Saris C., Tempst P., Ihle J.N., Schindler C.
      EMBO J. 14:1402-1411(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: PARTIAL PROTEIN SEQUENCE.
      Tissue: Mammary gland.

    Entry informationi

    Entry nameiSTA5A_SHEEP
    AccessioniPrimary (citable) accession number: P42231
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3