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P42231

- STA5A_SHEEP

UniProt

P42231 - STA5A_SHEEP

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Protein

Signal transducer and activator of transcription 5A

Gene

STAT5A

Organism
Ovis aries (Sheep)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.

GO - Molecular functioni

  1. calcium ion binding Source: InterPro
  2. DNA binding Source: AgBase
  3. sequence-specific DNA binding transcription factor activity Source: InterPro
  4. signal transducer activity Source: AgBase

GO - Biological processi

  1. cytokine-mediated signaling pathway Source: AgBase
  2. development of secondary female sexual characteristics Source: AgBase
  3. female pregnancy Source: AgBase
  4. JAK-STAT cascade Source: AgBase
  5. lactation Source: AgBase
  6. lipid storage Source: AgBase
  7. luteinization Source: AgBase
  8. mammary gland development Source: AgBase
  9. natural killer cell differentiation Source: AgBase
  10. negative regulation of apoptotic process Source: AgBase
  11. negative regulation of erythrocyte differentiation Source: AgBase
  12. positive regulation of activated T cell proliferation Source: AgBase
  13. positive regulation of B cell differentiation Source: AgBase
  14. positive regulation of cell proliferation Source: AgBase
  15. positive regulation of inflammatory response Source: AgBase
  16. positive regulation of interleukin-2 biosynthetic process Source: AgBase
  17. positive regulation of mitotic cell cycle Source: AgBase
  18. positive regulation of multicellular organism growth Source: AgBase
  19. positive regulation of transcription from RNA polymerase II promoter Source: AgBase
  20. prolactin signaling pathway Source: UniProtKB
  21. regulation of cell adhesion Source: AgBase
  22. regulation of epithelial cell differentiation Source: AgBase
  23. regulation of steroid metabolic process Source: AgBase
  24. T cell homeostasis Source: AgBase
  25. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Lactation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducer and activator of transcription 5A
Alternative name(s):
Mammary gland factor
Gene namesi
Name:STAT5A
Synonyms:MGF, STAT5
OrganismiOvis aries (Sheep)
Taxonomic identifieri9940 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeCaprinaeOvis
ProteomesiUP000002356: Unplaced

Subcellular locationi

Cytoplasm. Nucleus
Note: Translocated into the nucleus in response to phosphorylation.

GO - Cellular componenti

  1. cytoplasm Source: AgBase
  2. nucleus Source: AgBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 794794Signal transducer and activator of transcription 5APRO_0000182427Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei129 – 1291PhosphoserineBy similarity
Modified residuei694 – 6941Phosphotyrosine; by JAK2By similarity

Post-translational modificationi

Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated in response to signaling via activated FLT3 and KIT, resulting in translocation to the nucleus. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway (By similarity).By similarity
ISGylated.By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Expressioni

Tissue specificityi

Found in mammary gland and, in lesser extent, in ovary, thymus, spleen, kidney, lung, muscle and adrenal gland.

Inductioni

By prolactin.

Interactioni

Subunit structurei

Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1 and SOCS7. Interacts with ERBB4 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP42231.
SMRiP42231. Positions 139-690.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini589 – 68698SH2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the transcription factor STAT family.Curated
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain

Phylogenomic databases

HOVERGENiHBG107486.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
PANTHERiPTHR11801. PTHR11801. 1 hit.
PfamiPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
SMARTiSM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view]
SUPFAMiSSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42231-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN
60 70 80 90 100
PQDRAQVTQL LEGLVQELQK KAEHQVGEDG FLLKIKLGHY VHVSSRTRTT
110 120 130 140 150
AAPWSWLRCI RHILYNEQRL VREATNGNSS AGILVDAMSQ KHLQINQTFE
160 170 180 190 200
ELRLVTQDTE NELKKLQQTQ EYFIIQYQES LRIQAQFAQL AQLNPQERLS
210 220 230 240 250
RETALQQKQV SLEAWLQREA QTLQQYRVEL AEKHQKTLQL LRKQQTIILD
260 270 280 290 300
DELIQWKRRH DWRGMEAPPR SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC
310 320 330 340 350
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA
360 370 380 390 400
TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC
410 420 430 440 450
CVMEYHQRTG TLSAHFRNMS LKRIKRADRR GAESVTEEKF TVLFESQFSV
460 470 480 490 500
GSNELVFQVK TLSLPVVVIV HGSQDHNATA TVLWDNAFAE PGRVPFAVPD
510 520 530 540 550
KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL LFLAQKLFNN SSSHLEDYNG
560 570 580 590 600
MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK
610 620 630 640 650
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRE
660 670 680 690 700
GSIRSLADRL GDLNYLIYVF PDRPKDEVFS KYYTPVLAKA VDGYVKPQIK
710 720 730 740 750
QVVPEFVSAS ADSAGSSATY MDQAPSPAVC PQPHYNMYPQ NPDPVLDQDG
760 770 780 790
EFDLDETMDV ARHVEELLRR PNGQSGPLSP PPAGLFTPAR GSLS
Length:794
Mass (Da):90,917
Last modified:February 1, 1996 - v2
Checksum:iE69A4EC826AF60BE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78428 mRNA. Translation: CAA55191.1.
PIRiS55527.
RefSeqiNP_001009402.1. NM_001009402.1.
UniGeneiOar.13291.

Genome annotation databases

GeneIDi443419.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X78428 mRNA. Translation: CAA55191.1 .
PIRi S55527.
RefSeqi NP_001009402.1. NM_001009402.1.
UniGenei Oar.13291.

3D structure databases

ProteinModelPortali P42231.
SMRi P42231. Positions 139-690.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 443419.

Organism-specific databases

CTDi 6776.

Phylogenomic databases

HOVERGENi HBG107486.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view ]
PANTHERi PTHR11801. PTHR11801. 1 hit.
Pfami PF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view ]
SMARTi SM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view ]
SUPFAMi SSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEi PS50001. SH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Mammary gland factor (MGF) is a novel member of the cytokine regulated transcription factor gene family and confers the prolactin response."
    Wakao H., Gouilleux F., Groner B.
    EMBO J. 13:2182-2191(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Mammary gland.
  2. Erratum
    Wakao H., Gouilleux F., Groner B.
    EMBO J. 14:854-855(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION.
  3. "Interleukin-3 signals through multiple isoforms of Stat5."
    Azam M., Erdjument-Bromage H., Kreider B.L., Xia M., Quelle F., Basu R., Saris C., Tempst P., Ihle J.N., Schindler C.
    EMBO J. 14:1402-1411(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE.
    Tissue: Mammary gland.

Entry informationi

Entry nameiSTA5A_SHEEP
AccessioniPrimary (citable) accession number: P42231
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: February 1, 1996
Last modified: October 1, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3