Skip Header

 
Contribute Send feedback
Read comments (0) or add your own

Reviewed, UniProtKB/Swiss-Prot P42230 (STA5A_MOUSE)

Last modified July 7, 2009. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein namesRecommended name:
    Signal transducer and activator of transcription 5A
Alternative name(s):
    Mammary gland factor
Gene names
Name: Stat5a
Synonyms: Mgf, Mpf
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Hide | Top

Protein attributes

Sequence length793 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

Hide | Top

General annotation (Comments)

Function

Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Subunit structure

Forms a homodimer or a heterodimer with a related family member. Interacts with NCOA1 and SOCS7 By similarity. Binds NR3C1.

Subcellular location

Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Tissue specificity

In the virgin, found in most tissues except brain and muscle. During lactation, abundantly found in mammary tissue, as well as in other secretory organs such as salivary gland and seminal vesicle.

Post-translational modification

Tyrosine phosphorylated in response to IL-2, IL-3, IL-7, IL-15, GM-CSF, growth hormone, prolactin, erythropoietin and thrombopoietin. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity By similarity.

Sequence similarities

Belongs to the transcription factor STAT family.

Contains 1 SH2 domain.

Hide | Top

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainSH2 domain
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
   Technical term3D-structure
Gene Ontology (GO)
   Biological process2-oxoglutarate metabolic process

Inferred from mutant phenotype. Source: UniProtKB

JAK-STAT cascade involved in growth hormone signaling pathway

Inferred from direct assay. Source: UniProtKB

Peyer's patch development

Inferred from genetic interaction. Source: MGI

T cell differentiation in the thymus

Inferred from genetic interaction. Source: MGI

T cell homeostasis

Inferred from genetic interaction. Source: MGI

allantoin metabolic process

Inferred from mutant phenotype. Source: UniProtKB

citrate metabolic process

Inferred from mutant phenotype. Source: UniProtKB

creatine metabolic process

Inferred from mutant phenotype. Source: UniProtKB

creatinine metabolic process

Inferred from mutant phenotype. Source: UniProtKB

cytokine-mediated signaling pathway

Inferred from direct assay. Source: MGI

development of secondary female sexual characteristics

Inferred from mutant phenotype. Source: MGI

development of secondary male sexual characteristics

Inferred from genetic interaction. Source: MGI

fatty acid metabolic process

Inferred from mutant phenotype. Source: UniProtKB

female pregnancy

Inferred from genetic interaction. Source: MGI

isoleucine metabolic process

Inferred from mutant phenotype. Source: UniProtKB

lactation

Inferred from mutant phenotype. Source: MGI

lipid storage

Inferred from genetic interaction. Source: MGI

luteinization

Inferred from genetic interaction. Source: MGI

natural killer cell differentiation

Inferred from genetic interaction. Source: MGI

negative regulation of erythrocyte differentiation

Inferred from genetic interaction. Source: MGI

negative regulation of mast cell apoptosis

Inferred from mutant phenotype. Source: MGI

oxaloacetate metabolic process

Inferred from mutant phenotype. Source: UniProtKB

peptidyl-tyrosine phosphorylation

Inferred from direct assay. Source: MGI

positive regulation of B cell differentiation

Inferred from genetic interaction. Source: MGI

positive regulation of activated T cell proliferation

Inferred from direct assay. Source: MGI

positive regulation of gamma-delta T cell differentiation

Inferred from genetic interaction. Source: MGI

positive regulation of inflammatory response

Inferred from genetic interaction. Source: MGI

positive regulation of interleukin-2 biosynthetic process

Inferred from genetic interaction. Source: MGI

positive regulation of mast cell differentiation

Inferred from mutant phenotype. Source: MGI

positive regulation of mitotic cell cycle

Inferred from genetic interaction. Source: MGI

positive regulation of multicellular organism growth

Inferred from genetic interaction. Source: MGI

positive regulation of natural killer cell differentiation

Inferred from mutant phenotype. Source: MGI

positive regulation of natural killer cell mediated cytotoxicity

Inferred from mutant phenotype. Source: MGI

positive regulation of survival gene product expression

Inferred from genetic interaction. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from mutant phenotype. Source: MGI

regulation of cell adhesion

Inferred from mutant phenotype. Source: MGI

regulation of epithelial cell differentiation

Inferred from mutant phenotype. Source: MGI

regulation of steroid metabolic process

Inferred from genetic interaction. Source: MGI

succinate metabolic process

Inferred from mutant phenotype. Source: UniProtKB

taurine metabolic process

Inferred from mutant phenotype. Source: UniProtKB

transcription

Inferred from electronic annotation. Source: UniProtKB-KW

valine metabolic process

Inferred from mutant phenotype. Source: UniProtKB

   Cellular componentcytoplasm

Inferred from direct assay. Source: MGI

nucleus

Inferred from direct assay. Source: MGI

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: InterPro

phosphate binding

Inferred from direct assay. Source: UniProtKB

protein binding

Inferred from physical interaction. Source: UniProtKB

signal transducer activity

Inferred from genetic interaction. Source: MGI

transcription factor activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Hide | Top

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 793793Signal transducer and activator of transcription 5A
PRO_0000182424

Regions

Domain589 – 68698SH2

Amino acid modifications

Modified residue3721Phosphothreonine By similarity
Modified residue3751Phosphoserine By similarity
Modified residue6941Phosphotyrosine; by JAK Ref.5
Modified residue7791Phosphoserine By similarity

Secondary structure

1 ........................................................................ 793
Helix Strand Turn

Details...

Hide | Top

Sequences

Sequence LengthMass (Da)Tools
P42230-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7C66E435C37624DD

FASTA79390,831
        10         20         30         40         50         60 
MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN PQDRGQATQL 

        70         80         90        100        110        120 
LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQNTYDR CPMELVRCIR HILYNEQRLV 

       130        140        150        160        170        180 
REANNCSSPA GVLVDAMSQK HLQINQRFEE LRLITQDTEN ELKKLQQTQE YFIIQYQESL 

       190        200        210        220        230        240 
RIQAQFAQLG QLNPQERMSR ETALQQKQVS LETWLQREAQ TLQQYRVELA EKHQKTLQLL 

       250        260        270        280        290        300 
RKQQTIILDD ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 

       310        320        330        340        350        360 
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA TVRLLVGGKL 

       370        380        390        400        410        420 
NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC CVMEYHQATG TLSAHFRNMS 

       430        440        450        460        470        480 
LKRIKRADRR GAESVTEEKF TVLFESQFSV GSNELVFQVK TLSLPVVVIV HGSQDHNATA 

       490        500        510        520        530        540 
TVLWDNAFAE PGRVPFAVPD KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNI 

       550        560        570        580        590        600 
SSNHLEDYNS MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 

       610        620        630        640        650        660 
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRD FSIRSLADRL 

       670        680        690        700        710        720 
GDLNYLIYVF PDRPKDEVFA KYYTPVLAKA VDGYVKPQIK QVVPEFVNAS TDAGASATYM 

       730        740        750        760        770        780 
DQAPSPVVCP QPHYNMYPPN PDPVLDQDGE FDLDESMDVA RHVEELLRRP MDSLDARLSP 

       790 
PAGLFTSARS SLS

« Hide

Hide | Top

References

« Hide 'large scale' references
[1]"Interleukin-3, granulocyte-macrophage colony stimulating factor and interleukin-5 transduce signals through two STAT5 homologs."
Mui A.L.-F., Wakao H., O'Farrell A.-M., Harada N., Miyajima A.
EMBO J. 14:1166-1175(1995) [PubMed: 7720707] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6 X A/J.
Tissue: Liver.
[2]"Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue."
Liu X., Robinson G.W., Gouilleux F., Groner B., Hennighausen L.
Proc. Natl. Acad. Sci. U.S.A. 92:8831-8835(1995) [PubMed: 7568026] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6.
[3]Zhou L.X., Moore R.C., Oka T.
Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C3H/HeN.
Tissue: Mammary gland.
[4]"Characterization of Stat5a and Stat5b homodimers and heterodimers and their association with the glucocortiocoid receptor in mammary cells."
Cella N., Groner B., Hynes N.E.
Mol. Cell. Biol. 18:1783-1792(1998) [PubMed: 9528750] [Abstract]
Cited for: INTERACTION WITH NR3C1.
[5]"Multiple reaction monitoring for robust quantitative proteomic analysis of cellular signaling networks."
Wolf-Yadlin A., Hautaniemi S., Lauffenburger D.A., White F.M.
Proc. Natl. Acad. Sci. U.S.A. 104:5860-5865(2007) [PubMed: 17389395] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-694, MASS SPECTROMETRY.
[6]"Structure of the unphosphorylated STAT5a dimer."
Neculai D., Neculai A.M., Verrier S., Straub K., Klumpp K., Pfitzner E., Becker S.
J. Biol. Chem. 280:40782-40787(2005) [PubMed: 16192273] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS) OF 128-712.
+Additional computationally mapped references.

Hide | Top

Cross-references

Sequence databases

Z48538 mRNA. Translation: CAA88419.1.
U21103 mRNA. Translation: AAA80590.1.
U36502 mRNA. Translation: AAA78945.1.
IPIIPI00114979.
PIRS54772.
RefSeqNP_035618.1.
UniGeneMm.277403

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
1Y1UX-ray3.21A/B/C128-712[»]
ModBaseSearch...

Protein-protein interaction databases

DIPDIP:897N.
IntActP42230. 1 interaction.

PTM databases

PhosphoSiteP42230.

Proteomic databases

PRIDEP42230.

Genome annotation databases

EnsemblENSMUSG00000004043. Mus musculus. [Contig view]
GeneID20850.
KEGGmmu:20850.

Organism-specific databases

MGIMGI:103036. Stat5a.

Phylogenomic databases

HOGENOMP42230.
HOVERGENP42230.
OMAP42230. DSPERNL.

Gene expression databases

ArrayExpressP42230.
BgeeP42230.
GermOnlineENSMUSG00000004043. Mus musculus.

Family and domain databases

InterProIPR011992. EF-Hand_type.
IPR000980. SH2.
IPR013800. STAT_TF_alpha.
IPR001217. STAT_TF_core.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
Gene3DG3DSA:1.10.238.10. EF-Hand_type. 1 hit.
G3DSA:3.30.505.10. SH2. 1 hit.
G3DSA:1.20.1050.20. STAT_alpha. 1 hit.
G3DSA:2.60.40.630. STAT_DNA_bd_sub. 1 hit.
G3DSA:1.10.532.10. STAT_protein_interaction. 1 hit.
PANTHERPTHR11801. STAT. 1 hit.
PfamPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
ProDomPD000093. SH2. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00252. SH2. 1 hit.
[Graphical view]
PROSITEPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio299635.
SOURCESearch...

Hide | Top

Entry information

Entry nameSTA5A_MOUSE
AccessionPrimary (citable) accession number: P42230
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 7, 2009
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Hide | Top

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents