SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P42230

- STA5A_MOUSE

UniProt

P42230 - STA5A_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Signal transducer and activator of transcription 5A
Gene
Stat5a, Mgf, Mpf
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Carries out a dual function: signal transduction and activation of transcription. Mediates cellular responses to the cytokine KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the GAS element and activates PRL-induced transcription. Regulates the expression of milk proteins during lactation.2 Publications

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. RNA polymerase II core promoter sequence-specific DNA binding Source: MGI
  3. calcium ion binding Source: InterPro
  4. protein binding Source: IntAct
  5. protein phosphatase binding Source: UniProtKB
  6. sequence-specific DNA binding transcription factor activity Source: InterPro
  7. signal transducer activity Source: MGI

GO - Biological processi

  1. 2-oxoglutarate metabolic process Source: BHF-UCL
  2. JAK-STAT cascade Source: MGI
  3. JAK-STAT cascade involved in growth hormone signaling pathway Source: BHF-UCL
  4. Peyer's patch development Source: MGI
  5. T cell differentiation in thymus Source: MGI
  6. T cell homeostasis Source: MGI
  7. allantoin metabolic process Source: BHF-UCL
  8. cellular response to granulocyte macrophage colony-stimulating factor stimulus Source: MGI
  9. citrate metabolic process Source: BHF-UCL
  10. creatine metabolic process Source: BHF-UCL
  11. creatinine metabolic process Source: BHF-UCL
  12. cytokine-mediated signaling pathway Source: MGI
  13. development of secondary female sexual characteristics Source: MGI
  14. development of secondary male sexual characteristics Source: MGI
  15. epithelial cell differentiation involved in prostate gland development Source: MGI
  16. fatty acid metabolic process Source: BHF-UCL
  17. female pregnancy Source: MGI
  18. isoleucine metabolic process Source: BHF-UCL
  19. lactation Source: MGI
  20. lipid storage Source: MGI
  21. luteinization Source: MGI
  22. mammary gland development Source: MGI
  23. mammary gland epithelium development Source: MGI
  24. natural killer cell differentiation Source: MGI
  25. negative regulation of apoptotic process Source: MGI
  26. negative regulation of erythrocyte differentiation Source: MGI
  27. negative regulation of mast cell apoptotic process Source: MGI
  28. oxaloacetate metabolic process Source: BHF-UCL
  29. peptidyl-tyrosine phosphorylation Source: MGI
  30. positive regulation of B cell differentiation Source: MGI
  31. positive regulation of activated T cell proliferation Source: MGI
  32. positive regulation of cell proliferation Source: MGI
  33. positive regulation of gamma-delta T cell differentiation Source: MGI
  34. positive regulation of inflammatory response Source: MGI
  35. positive regulation of interleukin-2 biosynthetic process Source: MGI
  36. positive regulation of lymphocyte differentiation Source: MGI
  37. positive regulation of mast cell differentiation Source: MGI
  38. positive regulation of mast cell proliferation Source: MGI
  39. positive regulation of mitotic cell cycle Source: MGI
  40. positive regulation of multicellular organism growth Source: MGI
  41. positive regulation of natural killer cell differentiation Source: MGI
  42. positive regulation of natural killer cell mediated cytotoxicity Source: MGI
  43. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  44. prolactin signaling pathway Source: UniProtKB
  45. prostate gland epithelium morphogenesis Source: MGI
  46. regulation of cell adhesion Source: MGI
  47. regulation of epithelial cell differentiation Source: MGI
  48. regulation of multicellular organism growth Source: BHF-UCL
  49. regulation of steroid metabolic process Source: MGI
  50. regulation of transcription from RNA polymerase II promoter Source: MGI
  51. response to peptide hormone Source: MGI
  52. succinate metabolic process Source: BHF-UCL
  53. taurine metabolic process Source: BHF-UCL
  54. transcription, DNA-templated Source: UniProtKB-KW
  55. valine metabolic process Source: BHF-UCL
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Lactation, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_169390. Signaling by Leptin.
REACT_188529. Signaling by Leptin.
REACT_188578. Signaling by SCF-KIT.
REACT_196460. Signaling by FGFR1 fusion mutants.
REACT_198561. Prolactin receptor signaling.
REACT_198574. Nuclear signaling by ERBB4.
REACT_198614. Growth hormone receptor signaling.
REACT_198701. Interleukin-7 signaling.
REACT_225836. Interleukin-3, 5 and GM-CSF signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Signal transducer and activator of transcription 5A
Alternative name(s):
Mammary gland factor
Gene namesi
Name:Stat5a
Synonyms:Mgf, Mpf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:103036. Stat5a.

Subcellular locationi

Cytoplasm. Nucleus
Note: Translocated into the nucleus in response to phosphorylation.1 Publication

GO - Cellular componenti

  1. cytoplasm Source: MGI
  2. cytosol Source: Reactome
  3. nucleoplasm Source: Reactome
  4. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 793793Signal transducer and activator of transcription 5A
PRO_0000182424Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei128 – 1281Phosphoserine By similarity
Modified residuei694 – 6941Phosphotyrosine; by JAK2 By similarity

Post-translational modificationi

Tyrosine phosphorylated in response to IL2, IL3, IL7, IL15, KITLG/SCF, CSF2/GMCSF, GH1, PRL, EPO and THPO. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2 at Tyr-694. Tyrosine phosphorylation is required for DNA-binding activity and dimerization. Serine phosphorylation is also required for maximal transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway By similarity.4 Publications
ISGylated.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP42230.
PaxDbiP42230.
PRIDEiP42230.

PTM databases

PhosphoSiteiP42230.

Expressioni

Tissue specificityi

In the virgin, found in most tissues except brain and muscle. During lactation, abundantly found in mammary tissue, as well as in other secretory organs such as salivary gland and seminal vesicle.

Gene expression databases

ArrayExpressiP42230.
BgeeiP42230.
GenevestigatoriP42230.

Interactioni

Subunit structurei

Forms a homodimer or a heterodimer with a related family member. Interacts with NCOA1 and SOCS7 By similarity. Binds NR3C1. Interacts with ERBB4.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
AGAP2Q99490-22EBI-617434,EBI-7737644From a different organism.
GHRP199413EBI-617434,EBI-7526279From a different organism.

Protein-protein interaction databases

BioGridi203525. 13 interactions.
DIPiDIP-897N.
IntActiP42230. 7 interactions.
MINTiMINT-2576471.

Structurei

Secondary structure

Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi144 – 18138
Turni182 – 1909
Turni196 – 1983
Helixi199 – 24951
Helixi251 – 26212
Turni263 – 2653
Helixi273 – 30028
Helixi309 – 33022
Beta strandi332 – 3365
Beta strandi340 – 3456
Beta strandi348 – 3558
Turni356 – 3616
Beta strandi368 – 3758
Helixi376 – 3838
Beta strandi404 – 4063
Turni407 – 4104
Beta strandi411 – 4133
Beta strandi415 – 4195
Helixi435 – 4373
Beta strandi439 – 44911
Beta strandi451 – 4544
Beta strandi456 – 4627
Beta strandi466 – 4694
Helixi475 – 48814
Beta strandi500 – 5034
Helixi504 – 51916
Helixi527 – 53812
Helixi545 – 5506
Beta strandi552 – 5543
Helixi555 – 5595
Beta strandi566 – 5694
Helixi570 – 58415
Helixi586 – 5916
Helixi600 – 6089
Beta strandi615 – 6206
Beta strandi626 – 6316
Beta strandi641 – 6466
Helixi648 – 6536
Helixi656 – 6627
Helixi675 – 6795
Turni680 – 6823

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y1UX-ray3.21A/B/C128-712[»]
ProteinModelPortaliP42230.
SMRiP42230. Positions 138-690.

Miscellaneous databases

EvolutionaryTraceiP42230.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini589 – 68698SH2
Add
BLAST

Sequence similaritiesi

Contains 1 SH2 domain.

Keywords - Domaini

SH2 domain

Phylogenomic databases

eggNOGiNOG245085.
HOGENOMiHOG000230988.
HOVERGENiHBG107486.
InParanoidiP42230.
KOiK11223.
OrthoDBiEOG73JKTT.
TreeFamiTF318648.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
PANTHERiPTHR11801. PTHR11801. 1 hit.
PfamiPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
SMARTiSM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view]
SUPFAMiSSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEiPS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42230-1 [UniParc]FASTAAdd to Basket

« Hide

MAGWIQAQQL QGDALRQMQV LYGQHFPIEV RHYLAQWIES QPWDAIDLDN    50
PQDRGQATQL LEGLVQELQK KAEHQVGEDG FLLKIKLGHY ATQLQNTYDR 100
CPMELVRCIR HILYNEQRLV REANNCSSPA GVLVDAMSQK HLQINQRFEE 150
LRLITQDTEN ELKKLQQTQE YFIIQYQESL RIQAQFAQLG QLNPQERMSR 200
ETALQQKQVS LETWLQREAQ TLQQYRVELA EKHQKTLQLL RKQQTIILDD 250
ELIQWKRRQQ LAGNGGPPEG SLDVLQSWCE KLAEIIWQNR QQIRRAEHLC 300
QQLPIPGPVE EMLAEVNATI TDIISALVTS TFIIEKQPPQ VLKTQTKFAA 350
TVRLLVGGKL NVHMNPPQVK ATIISEQQAK SLLKNENTRN ECSGEILNNC 400
CVMEYHQATG TLSAHFRNMS LKRIKRADRR GAESVTEEKF TVLFESQFSV 450
GSNELVFQVK TLSLPVVVIV HGSQDHNATA TVLWDNAFAE PGRVPFAVPD 500
KVLWPQLCEA LNMKFKAEVQ SNRGLTKENL VFLAQKLFNI SSNHLEDYNS 550
MSVSWSQFNR ENLPGWNYTF WQWFDGVMEV LKKHHKPHWN DGAILGFVNK 600
QQAHDLLINK PDGTFLLRFS DSEIGGITIA WKFDSPDRNL WNLKPFTTRD 650
FSIRSLADRL GDLNYLIYVF PDRPKDEVFA KYYTPVLAKA VDGYVKPQIK 700
QVVPEFVNAS TDAGASATYM DQAPSPVVCP QPHYNMYPPN PDPVLDQDGE 750
FDLDESMDVA RHVEELLRRP MDSLDARLSP PAGLFTSARS SLS 793
Length:793
Mass (Da):90,831
Last modified:November 1, 1995 - v1
Checksum:i7C66E435C37624DD
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48538 mRNA. Translation: CAA88419.1.
U21103 mRNA. Translation: AAA80590.1.
U36502 mRNA. Translation: AAA78945.1.
CCDSiCCDS25439.1.
PIRiS54772.
RefSeqiNP_035618.1. NM_011488.3.
XP_006532784.1. XM_006532721.1.
XP_006532785.1. XM_006532722.1.
UniGeneiMm.277403.

Genome annotation databases

EnsembliENSMUST00000004145; ENSMUSP00000004145; ENSMUSG00000004043.
ENSMUST00000107356; ENSMUSP00000102979; ENSMUSG00000004043.
GeneIDi20850.
KEGGimmu:20850.
UCSCiuc007lml.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z48538 mRNA. Translation: CAA88419.1 .
U21103 mRNA. Translation: AAA80590.1 .
U36502 mRNA. Translation: AAA78945.1 .
CCDSi CCDS25439.1.
PIRi S54772.
RefSeqi NP_035618.1. NM_011488.3.
XP_006532784.1. XM_006532721.1.
XP_006532785.1. XM_006532722.1.
UniGenei Mm.277403.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Y1U X-ray 3.21 A/B/C 128-712 [» ]
ProteinModelPortali P42230.
SMRi P42230. Positions 138-690.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 203525. 13 interactions.
DIPi DIP-897N.
IntActi P42230. 7 interactions.
MINTi MINT-2576471.

Chemistry

ChEMBLi CHEMBL5513.

PTM databases

PhosphoSitei P42230.

Proteomic databases

MaxQBi P42230.
PaxDbi P42230.
PRIDEi P42230.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000004145 ; ENSMUSP00000004145 ; ENSMUSG00000004043 .
ENSMUST00000107356 ; ENSMUSP00000102979 ; ENSMUSG00000004043 .
GeneIDi 20850.
KEGGi mmu:20850.
UCSCi uc007lml.2. mouse.

Organism-specific databases

CTDi 6776.
MGIi MGI:103036. Stat5a.

Phylogenomic databases

eggNOGi NOG245085.
HOGENOMi HOG000230988.
HOVERGENi HBG107486.
InParanoidi P42230.
KOi K11223.
OrthoDBi EOG73JKTT.
TreeFami TF318648.

Enzyme and pathway databases

Reactomei REACT_169390. Signaling by Leptin.
REACT_188529. Signaling by Leptin.
REACT_188578. Signaling by SCF-KIT.
REACT_196460. Signaling by FGFR1 fusion mutants.
REACT_198561. Prolactin receptor signaling.
REACT_198574. Nuclear signaling by ERBB4.
REACT_198614. Growth hormone receptor signaling.
REACT_198701. Interleukin-7 signaling.
REACT_225836. Interleukin-3, 5 and GM-CSF signaling.

Miscellaneous databases

EvolutionaryTracei P42230.
NextBioi 299635.
PROi P42230.
SOURCEi Search...

Gene expression databases

ArrayExpressi P42230.
Bgeei P42230.
Genevestigatori P42230.

Family and domain databases

Gene3Di 1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProi IPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view ]
PANTHERi PTHR11801. PTHR11801. 1 hit.
Pfami PF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view ]
SMARTi SM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view ]
SUPFAMi SSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEi PS50001. SH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Interleukin-3, granulocyte-macrophage colony stimulating factor and interleukin-5 transduce signals through two STAT5 homologs."
    Mui A.L.-F., Wakao H., O'Farrell A.-M., Harada N., Miyajima A.
    EMBO J. 14:1166-1175(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X A/J.
    Tissue: Liver.
  2. "Cloning and expression of Stat5 and an additional homologue (Stat5b) involved in prolactin signal transduction in mouse mammary tissue."
    Liu X., Robinson G.W., Gouilleux F., Groner B., Hennighausen L.
    Proc. Natl. Acad. Sci. U.S.A. 92:8831-8835(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6.
  3. Zhou L.X., Moore R.C., Oka T.
    Submitted (SEP-1995) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C3H/HeN.
    Tissue: Mammary gland.
  4. "Characterization of Stat5a and Stat5b homodimers and heterodimers and their association with the glucocortiocoid receptor in mammary cells."
    Cella N., Groner B., Hynes N.E.
    Mol. Cell. Biol. 18:1783-1792(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH NR3C1.
  5. "ErbB4 signaling in the mammary gland is required for lobuloalveolar development and Stat5 activation during lactation."
    Jones F.E., Welte T., Fu X.Y., Stern D.F.
    J. Cell Biol. 147:77-88(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ERBB4.
  6. "Flt3 mutations from patients with acute myeloid leukemia induce transformation of 32D cells mediated by the Ras and STAT5 pathways."
    Mizuki M., Fenski R., Halfter H., Matsumura I., Schmidt R., Muller C., Gruning W., Kratz-Albers K., Serve S., Steur C., Buchner T., Kienast J., Kanakura Y., Berdel W.E., Serve H.
    Blood 96:3907-3914(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION IN RESPONSE TO FLT3 SIGNALING.
  7. "The intracellular domain of ErbB4 induces differentiation of mammary epithelial cells."
    Muraoka-Cook R.S., Sandahl M., Husted C., Hunter D., Miraglia L., Feng S.M., Elenius K., Earp H.S. III
    Mol. Biol. Cell 17:4118-4129(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, SUBCELLULAR LOCATION, INTERACTION WITH ERBB4.
  8. "Mechanisms of STAT protein activation by oncogenic KIT mutants in neoplastic mast cells."
    Chaix A., Lopez S., Voisset E., Gros L., Dubreuil P., De Sepulveda P.
    J. Biol. Chem. 286:5956-5966(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION IN RESPONSE TO KIT SIGNALING.
  9. Cited for: PHOSPHORYLATION IN RESPONSE TO FLT3 SIGNALING.
  10. Cited for: ISGYLATION.
  11. Cited for: X-RAY CRYSTALLOGRAPHY (3.21 ANGSTROMS) OF 128-712.

Entry informationi

Entry nameiSTA5A_MOUSE
AccessioniPrimary (citable) accession number: P42230
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 3, 2014
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi