Signal transducer and activator of transcription 4

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P42228 (STAT4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Signal transducer and activator of transcription 4

Gene names

Name:

Stat4

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	749 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Carries out a dual function: signal transduction and activation of transcription.
Subunit structure	Forms a homodimer or a heterodimer with a related family member By similarity. Interacts with ARL2BP. Ref.3
Subcellular location	Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.
Post-translational modification	Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity By similarity.
Sequence similarities	Belongs to the transcription factor STAT family. Contains 1 SH2 domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	SH2 domain
Ligand	DNA-binding
Molecular function	Activator
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell proliferation Inferred from direct assay PubMed 12213961. Source: MGI cytokine-mediated signaling pathway Inferred from direct assay PubMed 11894097 PubMed 12213961. Source: MGI positive regulation of transcription from RNA polymerase II promoter Inferred from direct assay PubMed 19666510. Source: MGI protein phosphorylation Inferred from direct assay PubMed 11489994. Source: MGI transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay PubMed 12213961. Source: MGI
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding Inferred from direct assay PubMed 11894097. Source: MGI sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro signal transducer activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Stat1	P42225	2	EBI-6253572,EBI-647118

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 749

749

Signal transducer and activator of transcription 4

PRO_0000182421

Regions

Domain

570 – 665

SH2

Amino acid modifications

Modified residue

694

Phosphotyrosine; by JAK By similarity

Experimental info

Sequence conflict

199

Missing in AAA19692. Ref.2

Sequence conflict

638

A → P in AAA19692. Ref.2

Secondary structure

749

Helix Strand Turn

Details...

Sequences

Sequence

Length

Mass (Da)

Tools

P42228 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A88B837E49FCFEBC
Show »

FASTA

749

85,941

        10         20         30         40         50         60 
MSQWNQVQQL EIKFLEQVDQ FYDDNFPMEI RHLLAQWIET QDWEVASNNE TMATILLQNL 

        70         80         90        100        110        120 
LIQLDEQLGR VSKEKNLLLI HNLKRIRKVL QGKFHGNPMH VAVVISNCLR EERRILAAAN 

       130        140        150        160        170        180 
MPIQGPLEKS LQSSSVSERQ RNVEHKVSAI KNSVQMTEQD TKYLEDLQDE FDYRYKTIQT 

       190        200        210        220        230        240 
MDQGDKNSIL VNQEVLTLLQ EMLNSLDFKR KEALSKMTQI VNETDLLMNS MLLEELQDWK 

       250        260        270        280        290        300 
KRQQIACIGG PLHNGLDQLQ NCFTLLAESL FQLRQQLEKL QEQSTKMTYE GDPIPAQRAH 

       310        320        330        340        350        360 
LLERATFLIY NLFKNSFVVE RQPCMPTHPQ RPMVLKTLIQ FTVKLRLLIK LPELNYQVKV 

       370        380        390        400        410        420 
KASIDKNVST LSNRRFVLCG THVKAMSSEE SSNGSLSVEF RHLQPKEMKC STGSKGNEGC 

       430        440        450        460        470        480 
HMVTEELHSI TFETQICLYG LTINLETSSL PVVMISNVSQ LPNAWASIIW YNVSTNDSQN 

       490        500        510        520        530        540 
LVFFNNPPSV TLGQLLEVMS WQFSSYVGRG LNSEQLNMLA EKLTVQSNYN DGHLTWAKFC 

       550        560        570        580        590        600 
KEHLPGKTFT FWTWLEAILD LIKKHILPLW IDGYIMGFVS KEKERLLLKD KMPGTFLLRF 

       610        620        630        640        650        660 
SESHLGGITF TWVDQSENGE VRFHSVEPYN KGRLSALAFA DILRDYKVIM AENIPENPLK 

       670        680        690        700        710        720 
YLYPDIPKDK AFGKHYSSQP CEVSRPTERG DKGYVPSVFI PISTIRSDST EPQSPSDLLP 

       730        740 
MSPSAYAVLR ENLSPTTIET AMNSPYSAE

« Hide

References

[1]	"Stat3 and Stat4: members of the family of signal transducers and activators of transcription." Zhong Z., Wen Z., Darnell J.E. Jr. Proc. Natl. Acad. Sci. U.S.A. 91:4806-4810(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Thymus.
[2]	"Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation." Yamamoto K., Quelle F.W., Thierfelder W.E., Kreider B.L., Gilbert D.J., Jenkins N.A., Copeland N.G., Silvennoinen O., Ihle J.N. Mol. Cell. Biol. 14:4342-4349(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Testis.
[3]	"BART is essential for nuclear retention of STAT3." Muromoto R., Sekine Y., Imoto S., Ikeda O., Okayama T., Sato N., Matsuda T. Int. Immunol. 20:395-403(2008) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH ARL2BP.
[4]	"Structure of the amino-terminal protein interaction domain of STAT-4." Vinkemeier U., Moarefi I., Darnell J.E. Jr., Kuriyan J. Science 279:1048-1052(1998) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 1-123.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U06923 mRNA. Translation: AAA19453.1.
U09351 mRNA. Translation: AAA19692.1.

IPI

IPI00114970.

PIR

A56047.

RefSeq

NP_035617.1. NM_011487.4.

UniGene

Mm.1550.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BGF	X-ray	1.45	A	2-123	[»]

ProteinModelPortal

P42228.

SMR

P42228. Positions 2-703.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1170N.

IntAct

P42228. 1 interaction.

PTM databases

PhosphoSite

P42228.

Proteomic databases

PaxDb

P42228.

PRIDE

P42228.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

GeneID

20849.

KEGG

mmu:20849.

Organism-specific databases

CTD

6775.

MGI

MGI:103062. Stat4.

Phylogenomic databases

eggNOG

NOG267627.

HOGENOM

HOG000220792.

HOVERGEN

HBG055669.

InParanoid

P42228.

K11222.

OrthoDB

EOG405S0F.

Gene expression databases

Genevestigator

P42228.

GermOnline

ENSMUSG00000062939. Mus musculus.

Family and domain databases

Gene3D

1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.

InterPro

IPR011992. EF-hand-like_dom.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR001217. STAT_TF_core.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]

PANTHER

PTHR11801. PTHR11801. 1 hit.

Pfam

PF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]

SMART

SM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view]

SUPFAM

SSF49417. P53_like_DNA_bnd. 1 hit.
SSF47655. STAT. 1 hit.
SSF48092. STAT. 1 hit.

PROSITE

PS50001. SH2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P42228.

NextBio

299631.

SOURCE

Search...

Entry information

Entry name

STAT4_MOUSE

Accession

Primary (citable) accession number: P42228

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Experimental info

Secondary structure

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents