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P42228 (STAT4_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Signal transducer and activator of transcription 4
Gene names
Name:Stat4
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length749 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Carries out a dual function: signal transduction and activation of transcription.

Subunit structure

Forms a homodimer or a heterodimer with a related family member By similarity. Interacts with ARL2BP. Ref.3

Subcellular location

Cytoplasm. Nucleus. Note: Translocated into the nucleus in response to phosphorylation.

Post-translational modification

Tyrosine phosphorylated. Serine phosphorylation is also required for maximal transcriptional activity By similarity.

Sequence similarities

Belongs to the transcription factor STAT family.

Contains 1 SH2 domain.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainSH2 domain
   LigandDNA-binding
   Molecular functionActivator
   PTMPhosphoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcell proliferation

Inferred from direct assay PubMed 12213961. Source: MGI

cellular response to organic substance

Inferred from direct assay PubMed 20399120. Source: MGI

cytokine-mediated signaling pathway

Inferred from direct assay PubMed 11894097PubMed 12213961. Source: MGI

positive regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 19666510. Source: MGI

protein phosphorylation

Inferred from direct assay PubMed 11489994. Source: MGI

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

nucleus

Inferred from direct assay PubMed 12213961. Source: MGI

   Molecular_functionDNA binding

Inferred from direct assay PubMed 12213961. Source: MGI

calcium ion binding

Inferred from electronic annotation. Source: InterPro

protein binding

Inferred from physical interaction Ref.3. Source: UniProtKB

sequence-specific DNA binding

Inferred from direct assay PubMed 11894097. Source: MGI

sequence-specific DNA binding transcription factor activity

Inferred from electronic annotation. Source: InterPro

signal transducer activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Stat1P422252EBI-6253572,EBI-647118

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 749749Signal transducer and activator of transcription 4
PRO_0000182421

Regions

Domain570 – 66596SH2

Amino acid modifications

Modified residue6941Phosphotyrosine; by JAK By similarity

Experimental info

Sequence conflict1991Missing in AAA19692. Ref.2
Sequence conflict6381A → P in AAA19692. Ref.2

Secondary structure

................... 749
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P42228 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: A88B837E49FCFEBC

FASTA74985,941
        10         20         30         40         50         60 
MSQWNQVQQL EIKFLEQVDQ FYDDNFPMEI RHLLAQWIET QDWEVASNNE TMATILLQNL 

        70         80         90        100        110        120 
LIQLDEQLGR VSKEKNLLLI HNLKRIRKVL QGKFHGNPMH VAVVISNCLR EERRILAAAN 

       130        140        150        160        170        180 
MPIQGPLEKS LQSSSVSERQ RNVEHKVSAI KNSVQMTEQD TKYLEDLQDE FDYRYKTIQT 

       190        200        210        220        230        240 
MDQGDKNSIL VNQEVLTLLQ EMLNSLDFKR KEALSKMTQI VNETDLLMNS MLLEELQDWK 

       250        260        270        280        290        300 
KRQQIACIGG PLHNGLDQLQ NCFTLLAESL FQLRQQLEKL QEQSTKMTYE GDPIPAQRAH 

       310        320        330        340        350        360 
LLERATFLIY NLFKNSFVVE RQPCMPTHPQ RPMVLKTLIQ FTVKLRLLIK LPELNYQVKV 

       370        380        390        400        410        420 
KASIDKNVST LSNRRFVLCG THVKAMSSEE SSNGSLSVEF RHLQPKEMKC STGSKGNEGC 

       430        440        450        460        470        480 
HMVTEELHSI TFETQICLYG LTINLETSSL PVVMISNVSQ LPNAWASIIW YNVSTNDSQN 

       490        500        510        520        530        540 
LVFFNNPPSV TLGQLLEVMS WQFSSYVGRG LNSEQLNMLA EKLTVQSNYN DGHLTWAKFC 

       550        560        570        580        590        600 
KEHLPGKTFT FWTWLEAILD LIKKHILPLW IDGYIMGFVS KEKERLLLKD KMPGTFLLRF 

       610        620        630        640        650        660 
SESHLGGITF TWVDQSENGE VRFHSVEPYN KGRLSALAFA DILRDYKVIM AENIPENPLK 

       670        680        690        700        710        720 
YLYPDIPKDK AFGKHYSSQP CEVSRPTERG DKGYVPSVFI PISTIRSDST EPQSPSDLLP 

       730        740 
MSPSAYAVLR ENLSPTTIET AMNSPYSAE 

« Hide

References

[1]"Stat3 and Stat4: members of the family of signal transducers and activators of transcription."
Zhong Z., Wen Z., Darnell J.E. Jr.
Proc. Natl. Acad. Sci. U.S.A. 91:4806-4810(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Thymus.
[2]"Stat4, a novel gamma interferon activation site-binding protein expressed in early myeloid differentiation."
Yamamoto K., Quelle F.W., Thierfelder W.E., Kreider B.L., Gilbert D.J., Jenkins N.A., Copeland N.G., Silvennoinen O., Ihle J.N.
Mol. Cell. Biol. 14:4342-4349(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Testis.
[3]"BART is essential for nuclear retention of STAT3."
Muromoto R., Sekine Y., Imoto S., Ikeda O., Okayama T., Sato N., Matsuda T.
Int. Immunol. 20:395-403(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH ARL2BP.
[4]"Structure of the amino-terminal protein interaction domain of STAT-4."
Vinkemeier U., Moarefi I., Darnell J.E. Jr., Kuriyan J.
Science 279:1048-1052(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.45 ANGSTROMS) OF 1-123.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U06923 mRNA. Translation: AAA19453.1.
U09351 mRNA. Translation: AAA19692.1.
PIRA56047.
RefSeqNP_035617.1. NM_011487.4.
UniGeneMm.1550.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1BGFX-ray1.45A2-123[»]
ProteinModelPortalP42228.
SMRP42228. Positions 2-703.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid203524. 1 interaction.
DIPDIP-1170N.
IntActP42228. 1 interaction.

PTM databases

PhosphoSiteP42228.

Proteomic databases

PaxDbP42228.
PRIDEP42228.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID20849.
KEGGmmu:20849.

Organism-specific databases

CTD6775.
MGIMGI:103062. Stat4.

Phylogenomic databases

eggNOGNOG267627.
HOGENOMHOG000220792.
HOVERGENHBG055669.
InParanoidP42228.
KOK11222.
PhylomeDBP42228.

Gene expression databases

GenevestigatorP42228.

Family and domain databases

Gene3D1.10.238.10. 1 hit.
1.10.532.10. 1 hit.
1.20.1050.20. 1 hit.
2.60.40.630. 1 hit.
3.30.505.10. 1 hit.
InterProIPR011992. EF-hand-dom_pair.
IPR008967. p53-like_TF_DNA-bd.
IPR000980. SH2.
IPR001217. STAT.
IPR013800. STAT_TF_alpha.
IPR015988. STAT_TF_coiled-coil.
IPR013801. STAT_TF_DNA-bd.
IPR012345. STAT_TF_DNA-bd_sub.
IPR013799. STAT_TF_prot_interaction.
[Graphical view]
PANTHERPTHR11801. PTHR11801. 1 hit.
PfamPF00017. SH2. 1 hit.
PF01017. STAT_alpha. 1 hit.
PF02864. STAT_bind. 1 hit.
PF02865. STAT_int. 1 hit.
[Graphical view]
SMARTSM00252. SH2. 1 hit.
SM00964. STAT_int. 1 hit.
[Graphical view]
SUPFAMSSF47655. SSF47655. 1 hit.
SSF48092. SSF48092. 1 hit.
SSF49417. SSF49417. 1 hit.
SSF55550. SSF55550. 1 hit.
PROSITEPS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP42228.
NextBio299631.
PROP42228.
SOURCESearch...

Entry information

Entry nameSTAT4_MOUSE
AccessionPrimary (citable) accession number: P42228
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot