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Protein

Enolase-related protein 3

Gene

ERR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2), Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3), Phosphoglycerate mutase 1 (GPM1)
  4. Enolase 2 (ENO2), Enolase-related protein 2 (ERR2), Enolase-related protein 1 (ERR1), Enolase-related protein 3 (ERR3), Enolase 1 (ENO1)
  5. Pyruvate kinase 2 (PYK2), Pyruvate kinase 1 (CDC19)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160SubstrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei212Proton donorBy similarity1
Metal bindingi247MagnesiumBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Metal bindingi321MagnesiumBy similarity1
Binding sitei321SubstrateBy similarity1
Active sitei346Proton acceptorBy similarity1
Binding sitei397SubstrateBy similarity1

GO - Molecular functioni

  • magnesium ion binding Source: InterPro
  • phosphopyruvate hydratase activity Source: SGD

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processGlycolysis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YMR323W-MONOMER
SABIO-RKiP42222
UniPathwayiUPA00109; UER00187

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-related protein 3 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ERR3
Ordered Locus Names:YMR323W
ORF Names:YM9924.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR323W
SGDiS000004942 ERR3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340651 – 437Enolase-related protein 3Add BLAST437

Proteomic databases

PaxDbiP42222
PRIDEiP42222

Interactioni

Protein-protein interaction databases

BioGridi35502, 5 interactors
DIPiDIP-4046N
IntActiP42222, 9 interactors
MINTiP42222
STRINGi4932.YMR323W

Structurei

3D structure databases

ProteinModelPortaliP42222
SMRiP42222
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni373 – 376Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

GeneTreeiENSGT00910000144064
HOGENOMiHOG000072174
InParanoidiP42222
KOiK01689
OrthoDBiEOG092C2W5X

Family and domain databases

CDDicd03313 enolase, 1 hit
Gene3Di3.20.20.120, 1 hit
3.30.390.10, 1 hit
HAMAPiMF_00318 Enolase, 1 hit
InterProiView protein in InterPro
IPR000941 Enolase
IPR036849 Enolase-like_C_sf
IPR029017 Enolase-like_N
IPR034390 Enolase-like_superfamily
IPR020810 Enolase_C
IPR020809 Enolase_CS
IPR020811 Enolase_N
PANTHERiPTHR11902 PTHR11902, 1 hit
PfamiView protein in Pfam
PF00113 Enolase_C, 1 hit
PF03952 Enolase_N, 1 hit
PIRSFiPIRSF001400 Enolase, 1 hit
PRINTSiPR00148 ENOLASE
SFLDiSFLDG00178 enolase, 1 hit
SFLDS00001 Enolase, 1 hit
SMARTiView protein in SMART
SM01192 Enolase_C, 1 hit
SM01193 Enolase_N, 1 hit
SUPFAMiSSF51604 SSF51604, 1 hit
TIGRFAMsiTIGR01060 eno, 1 hit
PROSITEiView protein in PROSITE
PS00164 ENOLASE, 1 hit

Sequencei

Sequence statusi: Complete.

P42222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSITKVHART VYDSRGNPTV EVEITTENGL FRAIVPSGAS TGIHEAVELR
60 70 80 90 100
DGNKSEWMGK GVTKAVSNVN SIIGPALIKS DLCVTNQKGI DELMISLDGT
110 120 130 140 150
SNKSRLGANA ILGVSLCVAR AAAAQKGITL YKYIAELADA RQDPFVIPVP
160 170 180 190 200
FFNVLNGGAH AGGSLAMQEF KIAPVGAQSF AEAMRMGSEV YHHLKILAKE
210 220 230 240 250
QYGPSAGNVG DEGGVAPDID TAEDALDMIV KAINICGYEG RVKVGIDSAP
260 270 280 290 300
SVFYKDGKYD LNFKEPNSDP SHWLSPAQLA EYYHSLLKKY PIISLEDPYA
310 320 330 340 350
EDDWSSWSAF LKTVNVQIIA DDLTCTNKTR IARAIEEKCA NTLLLKLNQI
360 370 380 390 400
GTLTESIEAA NQAFDAGWGV MISHRSGETE DPFIADLVVG LRCGQIKSGA
410 420 430
LSRSERLAKY NELLRIEEEL GDDCIYAGHR FHDGNKL
Length:437
Mass (Da):47,313
Last modified:October 1, 1996 - v2
Checksum:i143D6EF66FB03D13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA Translation: CAA90841.1
BK006946 Genomic DNA Translation: DAA10224.1
PIRiS69881
RefSeqiNP_014056.3, NM_001182836.3

Genome annotation databases

EnsemblFungiiYMR323W; YMR323W; YMR323W
GeneIDi855373
KEGGisce:YMR323W

Similar proteinsi

Entry informationi

Entry nameiERR3_YEAST
AccessioniPrimary (citable) accession number: P42222
Secondary accession number(s): D6W0F0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: March 28, 2018
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health