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Protein

Enolase-related protein 3

Gene

ERR3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

2-phospho-D-glycerate = phosphoenolpyruvate + H2O.

Cofactori

Mg2+By similarity

Pathwayi: glycolysis

This protein is involved in step 4 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 1 (TDH1), Glyceraldehyde-3-phosphate dehydrogenase 2 (TDH2), Glyceraldehyde-3-phosphate dehydrogenase 3 (TDH3)
  2. Phosphoglycerate kinase (PGK1)
  3. Phosphoglycerate mutase 2 (GPM2), Phosphoglycerate mutase 3 (GPM3), Phosphoglycerate mutase 1 (GPM1)
  4. Enolase 2 (ENO2), Enolase-related protein 2 (ERR2), Enolase-related protein 1 (ERR1), Enolase-related protein 3 (ERR3), Enolase 1 (ENO1)
  5. Pyruvate kinase 2 (PYK2), Pyruvate kinase 1 (CDC19)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei160SubstrateBy similarity1
Binding sitei169SubstrateBy similarity1
Active sitei212Proton donorBy similarity1
Metal bindingi247MagnesiumBy similarity1
Metal bindingi296MagnesiumBy similarity1
Binding sitei296SubstrateBy similarity1
Metal bindingi321MagnesiumBy similarity1
Binding sitei321SubstrateBy similarity1
Active sitei346Proton acceptorBy similarity1
Binding sitei397SubstrateBy similarity1

GO - Molecular functioni

  • magnesium ion binding Source: InterPro
  • phosphopyruvate hydratase activity Source: SGD

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:YMR323W-MONOMER.
SABIO-RKP42222.
UniPathwayiUPA00109; UER00187.

Names & Taxonomyi

Protein namesi
Recommended name:
Enolase-related protein 3 (EC:4.2.1.11)
Alternative name(s):
2-phospho-D-glycerate hydro-lyase
2-phosphoglycerate dehydratase
Gene namesi
Name:ERR3
Ordered Locus Names:YMR323W
ORF Names:YM9924.15
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome XIII

Organism-specific databases

EuPathDBiFungiDB:YMR323W.
SGDiS000004942. ERR3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001340651 – 437Enolase-related protein 3Add BLAST437

Proteomic databases

PRIDEiP42222.

Interactioni

Protein-protein interaction databases

BioGridi35502. 5 interactors.
DIPiDIP-4046N.
MINTiMINT-2492619.

Structurei

3D structure databases

ProteinModelPortaliP42222.
SMRiP42222.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni373 – 376Substrate bindingBy similarity4

Sequence similaritiesi

Belongs to the enolase family.Curated

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiP42222.
KOiK01689.
OMAiCESGHAC.
OrthoDBiEOG092C2W5X.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42222-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSITKVHART VYDSRGNPTV EVEITTENGL FRAIVPSGAS TGIHEAVELR
60 70 80 90 100
DGNKSEWMGK GVTKAVSNVN SIIGPALIKS DLCVTNQKGI DELMISLDGT
110 120 130 140 150
SNKSRLGANA ILGVSLCVAR AAAAQKGITL YKYIAELADA RQDPFVIPVP
160 170 180 190 200
FFNVLNGGAH AGGSLAMQEF KIAPVGAQSF AEAMRMGSEV YHHLKILAKE
210 220 230 240 250
QYGPSAGNVG DEGGVAPDID TAEDALDMIV KAINICGYEG RVKVGIDSAP
260 270 280 290 300
SVFYKDGKYD LNFKEPNSDP SHWLSPAQLA EYYHSLLKKY PIISLEDPYA
310 320 330 340 350
EDDWSSWSAF LKTVNVQIIA DDLTCTNKTR IARAIEEKCA NTLLLKLNQI
360 370 380 390 400
GTLTESIEAA NQAFDAGWGV MISHRSGETE DPFIADLVVG LRCGQIKSGA
410 420 430
LSRSERLAKY NELLRIEEEL GDDCIYAGHR FHDGNKL
Length:437
Mass (Da):47,313
Last modified:October 1, 1996 - v2
Checksum:i143D6EF66FB03D13
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA. Translation: CAA90841.1.
BK006946 Genomic DNA. Translation: DAA10224.1.
PIRiS69881.
RefSeqiNP_014056.3. NM_001182836.3.

Genome annotation databases

EnsemblFungiiYMR323W; YMR323W; YMR323W.
GeneIDi855373.
KEGGisce:YMR323W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z54141 Genomic DNA. Translation: CAA90841.1.
BK006946 Genomic DNA. Translation: DAA10224.1.
PIRiS69881.
RefSeqiNP_014056.3. NM_001182836.3.

3D structure databases

ProteinModelPortaliP42222.
SMRiP42222.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi35502. 5 interactors.
DIPiDIP-4046N.
MINTiMINT-2492619.

Proteomic databases

PRIDEiP42222.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYMR323W; YMR323W; YMR323W.
GeneIDi855373.
KEGGisce:YMR323W.

Organism-specific databases

EuPathDBiFungiDB:YMR323W.
SGDiS000004942. ERR3.

Phylogenomic databases

HOGENOMiHOG000072174.
InParanoidiP42222.
KOiK01689.
OMAiCESGHAC.
OrthoDBiEOG092C2W5X.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00187.
BioCyciYEAST:YMR323W-MONOMER.
SABIO-RKP42222.

Miscellaneous databases

PROiP42222.

Family and domain databases

CDDicd03313. enolase. 1 hit.
Gene3Di3.20.20.120. 1 hit.
3.30.390.10. 1 hit.
HAMAPiMF_00318. Enolase. 1 hit.
InterProiIPR000941. Enolase.
IPR020810. Enolase_C.
IPR029065. Enolase_C-like.
IPR020809. Enolase_CS.
IPR020811. Enolase_N.
IPR029017. Enolase_N-like.
[Graphical view]
PANTHERiPTHR11902. PTHR11902. 1 hit.
PfamiPF00113. Enolase_C. 1 hit.
PF03952. Enolase_N. 1 hit.
[Graphical view]
PIRSFiPIRSF001400. Enolase. 1 hit.
PRINTSiPR00148. ENOLASE.
SMARTiSM01192. Enolase_C. 1 hit.
SM01193. Enolase_N. 1 hit.
[Graphical view]
SUPFAMiSSF51604. SSF51604. 1 hit.
SSF54826. SSF54826. 1 hit.
TIGRFAMsiTIGR01060. eno. 1 hit.
PROSITEiPS00164. ENOLASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiERR3_YEAST
AccessioniPrimary (citable) accession number: P42222
Secondary accession number(s): D6W0F0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome XIII
    Yeast (Saccharomyces cerevisiae) chromosome XIII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.