P42219 (CEFF_NOCLA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deacetoxycephalosporin C hydroxylase
EC=1.14.11.26

Alternative name(s):
Beta-lactam hydroxylase
Deacetylcephalosporin C synthase
Short name=DACS

Gene names

Name:

cefF

Organism

Nocardia lactamdurans

Taxonomic identifier

1913 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Pseudonocardineae › Pseudonocardiaceae › Amycolatopsis

Protein attributes

Sequence length	310 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydroxylation of desacetoxicephalosporin C in 3'position to form deacetylcephalosporin C.
Catalytic activity	Deacetoxycephalosporin C + 2-oxoglutarate + O2 = deacetylcephalosporin C + succinate + CO2.
Cofactor	Iron.
Pathway	Antibiotic biosynthesis; cephalosporin C biosynthesis.
Sequence similarities	Belongs to the iron/ascorbate-dependent oxidoreductase family. Contains 1 Fe2OG dioxygenase domain.

Ontologies

Keywords
Biological process	Antibiotic biosynthesis
Ligand	Iron Vitamin C
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	antibiotic biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	L-ascorbic acid binding Inferred from electronic annotation. Source: UniProtKB-KW deacetoxycephalosporin-C hydroxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 310	310	Deacetoxycephalosporin C hydroxylase		PRO_0000219512
Regions
	Domain	154 – 267	114	Fe2OG dioxygenase

Sequences

	Sequence		Length	Mass (Da)
	P42219 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 9983EF1AC1B416E6	FASTA	310	34,364
	10 20 30 40 50 60 MSDKTVPVFS MAELRDGSRQ DEFREWARRG VFYLTGYGAT ERDHRVATDT AMDFFAQGTA 70 80 90 100 110 120 EEKQAVTTKV PTMRRGYSAL EAESTAQVTN TGTYTDYSMS YSMGIGGNLF PSKEFESVWT 130 140 150 160 170 180 DYFDSLYRAA QETARLVLTA AGTYDGEDLD TLLDCDPVLR LRYFPEVPEH RAAEYEPRRM 190 200 210 220 230 240 APHYDLSIIT FIHQTPCANG FVSLQAEVDG EMVSLPHVED AVVVLCGAIA PLVTQGAVPA 250 260 270 280 290 300 PNHHVVSPDA SMLKGSDRTS SVFFLRPSTD FTFSVPDARK YGLDVSLDME KATFGDWIGT 310 NYVTMHAVTS

References

[1]	"Characterization of the cefF gene of Nocardia lactamdurans encoding a 3'-methylcephem hydroxylase different from the 7-cephem hydroxylase." Coque J., Enguita F.J., Cardoza R.E., Martin J.F., Liras P. Appl. Microbiol. Biotechnol. 44:605-609(1996) [PubMed: 8703431] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z21687 Genomic DNA. Translation: CAA79803.1.
PIR	S40253.
3D structure databases
ProteinModelPortal	P42219.
SMR	P42219. Positions 3-307.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005123. Oxoglutarate/Fe-dep_oxygenase. [Graphical view]
Pfam	PF03171. 2OG-FeII_Oxy. 1 hit. [Graphical view]
PROSITE	PS51471. FE2OG_OXY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CEFF_NOCLA

Accession

Primary (citable) accession number: P42219

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 28, 2011
This is version 53 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program