##gff-version 3
P42212	UniProtKB	Chain	1	238	.	.	.	ID=PRO_0000192576;Note=Green fluorescent protein	
P42212	UniProtKB	Modified residue	66	66	.	.	.	Note=(Z)-2%2C3-didehydrotyrosine;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8448132;Dbxref=PMID:8448132	
P42212	UniProtKB	Cross-link	65	67	.	.	.	Note=5-imidazolinone (Ser-Gly);Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8448132;Dbxref=PMID:8448132	
P42212	UniProtKB	Natural variant	100	100	.	.	.	Note=F->Y;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1347277;Dbxref=PMID:1347277	
P42212	UniProtKB	Natural variant	108	108	.	.	.	Note=T->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1347277;Dbxref=PMID:1347277	
P42212	UniProtKB	Natural variant	141	141	.	.	.	Note=L->M;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1347277;Dbxref=PMID:1347277	
P42212	UniProtKB	Natural variant	219	219	.	.	.	Note=V->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1347277;Dbxref=PMID:1347277	
P42212	UniProtKB	Mutagenesis	30	30	.	.	.	Note=In mut1.28%3B shifts fluorescence lifetime from 3.03 to 2.76 ns%3B when associated with H-145. In mut2.2%3B shifts fluorescence lifetime from 3.03 to 1.94 ns%3B when associated with H-69 and H-145. In mut3.3%3B shifts fluorescence lifetime from 3.03 to 1.88 ns%3B when associated with L-46%3B H-69 and H-145. In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with N-39%3B H-66%3B A-72%3B T-105%3B F-145%3B V-171%3B S-198 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. S->R;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16201762,ECO:0000269|PubMed:22891786;Dbxref=PMID:16201762,PMID:22891786	
P42212	UniProtKB	Mutagenesis	39	39	.	.	.	Note=In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B H-66%3B A-72%3B T-105%3B F-145%3B V-171%3B S-198 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. Y->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	46	46	.	.	.	Note=In mut3.3%3B shifts fluorescence lifetime from 3.03 to 1.88 ns%3B when associated with R-30%3B H-69 and H-145. In R10-3%3B matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm%2C respectively%3B when associated with L-64%3B N-68%3B Q-162%3B A-163%3B V-167 and L-171. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-64%3B G-65%3B L-68%3B A-72%3B T-153%3B A-163%3B G-175 and Y-203. F->L;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:16201762,ECO:0000269|PubMed:18366185;Dbxref=PMID:12370172,PMID:16201762,PMID:18366185	
P42212	UniProtKB	Mutagenesis	64	64	.	.	.	Note=In EGFP%3B increases fluorescence at warmer temperatures such as 37 degrees Celsius%3B when associated with T-65. In EBFP%3B gives rise to variants with blue fluorescence%3B when associated with T-65 and H-66. In ECFP%3B leads to cyan fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with T-65%3B W-66%3B I-146%3B T-153 and A-163. In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighterH than ECFP%3B when associated with T-65%3B W-66%3B A-72%3B A-145%3B I-146%3B D-148%3B T-153 and A-163. In R10-3%3B matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm%2C respectively%3B when associated with L-46%3B N-68%3B Q-162%3B A-163%3B V-167 and L-171. In GFPmut 1%3B red-shifts by about 100 nm the excitation maxima%2C permitting efficient excitation at 488 nm and increases fluorescence%3B when associated with T-65. In VisGreen%3B leads to brighter fluorescence%3B when associated with T-65%3B A-72%3B K-149 and T-167. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B G-65%3B L-68%3B A-72%3B T-153%3B A-163%3B G-175 and Y-203. F->L;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:11776264,ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:14990965,ECO:0000269|PubMed:15065907,ECO:0000269|PubMed:17658219,ECO:0000269|PubMed:18366185,ECO:0000269|PubMed:8707053,ECO:0000269|PubMed:9145105,ECO:0000269|PubMed:9370472;Dbxref=PMID:11299042,PMID:11776264,PMID:12370172,PMID:14990965,PMID:15065907,PMID:17658219,PMID:18366185,PMID:8707053,PMID:9145105,PMID:9370472	
P42212	UniProtKB	Mutagenesis	64	64	.	.	.	Note=In RSGFP4%3B increases fluorescence and shifts the major exitation peak to 489-490 nm%3B when associated with G-65 and L-69. F->M;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9634755;Dbxref=PMID:9634755	
P42212	UniProtKB	Mutagenesis	65	66	.	.	.	Note=Gives rise to variants with cerulean fluorsecence. SY->TW;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:17685554;Dbxref=PMID:17685554	
P42212	UniProtKB	Mutagenesis	65	65	.	.	.	Note=In GFPmut 2%3B red-shifts by about 100 nm the excitation maxima%2C permitting efficient excitation at 488 nm and increases fluorescence%3B when associated with L-68 and A-72. S->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8707053;Dbxref=PMID:8707053	
P42212	UniProtKB	Mutagenesis	65	65	.	.	.	Note=In EYFP%3B leads to yellow fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with L-68%3B A-72 and Y-203. In GFPmut 3%3B highly fluorescent mutant when excited at 488 nm%3B when associated with A-72. Highly fluorescent%3B when associated with Y-203%3B L-68 and A-72. In RSGFP4%3B increases fluorescence and shifts the major exitation peak to 489-490 nm%3B when associated with M-64 and L-69. In YFP 10C%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with L-68%3B A-72 and Y-203. In Topaz%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with A-72%3B R-79 and Y-203. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B L-64%3B L-68%3B A-72%3B T-153%3B A-163%3B G-175 and Y-203. S->G;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:8707053,ECO:0000269|PubMed:9634755,ECO:0000269|PubMed:9759496,ECO:0000269|PubMed:9782051;Dbxref=PMID:11299042,PMID:12370172,PMID:8707053,PMID:9634755,PMID:9759496,PMID:9782051	
P42212	UniProtKB	Mutagenesis	65	65	.	.	.	Note=Increases fluorescence%2C photostability and shifts the major exitation peak to 488 nm. In EGFP%3B increases fluorescence at warmer temperatures such as 37 degrees Celsius%3B when associated with L-64. In EBFP%3B gives rise to variants with blue fluorescence%3B when associated with L-64 and H-66. In GFPmut 1%3B red-shifts by about 100 nm the excitation maxima%2C permitting efficient excitation at 488 nm and increases fluorescence%3B when associated with T-65. In ECFP%3B leads to cyan fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with L-64%3B W-66%3B I-146%3B T-153 and A-163. In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B W-66%3B A-72%3B A-145%3B I-146%3B D-148%3B T-153 and A-163. In Esmerald%3B leads to brighter fluorescence%3B when associated with A-72%3B K-149%3B T-153 and T-167. In VisGreen%3B leads to brighter fluorescence%3B when associated with L-64%3B A-72%3B K-149 and T-167. S->T;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:11776264,ECO:0000269|PubMed:14990965,ECO:0000269|PubMed:15065907,ECO:0000269|PubMed:17658219,ECO:0000269|PubMed:8703075,ECO:0000269|PubMed:8707053,ECO:0000269|PubMed:9370472;Dbxref=PMID:11299042,PMID:11776264,PMID:14990965,PMID:15065907,PMID:17658219,PMID:8703075,PMID:8707053,PMID:9370472	
P42212	UniProtKB	Mutagenesis	66	66	.	.	.	Note=In BFP%3B shifts the excitation and emission spectra to shorter wavelengths. In EBFP%3B gives rise to variants with blue fluorescence%3B when associated with L-64 and T-65. In Azurite%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-80%3B F-145%3B I-150 and R-224. In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B A-72%3B T-105%3B F-145%3B V-171%3B S-198 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. Y->H;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:22891786,ECO:0000269|PubMed:8578587,ECO:0000269|PubMed:9145105,ECO:0000269|PubMed:9370472;Dbxref=PMID:22891786,PMID:8578587,PMID:9145105,PMID:9370472	
P42212	UniProtKB	Mutagenesis	66	66	.	.	.	Note=Shifts the excitation and emission spectra to shorter wavelengths. Y->T%2CF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8578587;Dbxref=PMID:8578587	
P42212	UniProtKB	Mutagenesis	66	66	.	.	.	Note=In W%3B leads to excitation and emission wavelengths intermediate between tyrosine and histidine but is only weakly fluorescent. In ECFP%3B leads to cyan fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with L-64%3B T-65%3B I-146%3B T-153 and A-163. In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B T-65%3B A-72%3B A-145%3B I-146%3B D-148%3B T-153 and A-163. Y->W;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:14990965,ECO:0000269|PubMed:15065907,ECO:0000269|PubMed:7809066;Dbxref=PMID:11299042,PMID:14990965,PMID:15065907,PMID:7809066	
P42212	UniProtKB	Mutagenesis	68	68	.	.	.	Note=In EYFP%3B leads to yellow fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with G-65%3B A-72 and Y-203. In GFPmut 2%3B red-shifts by about 100 nm the excitation maxima%2C permitting efficient excitation at 488 nm and increases fluorescence%3B when associated with A-65 and A-72. In Citrinine%3B leads to excitation and emission peaks of 516 and 529 nm%2C respectively%3B when associated with M-69%3B A-72 and Y-203. In YFP 10C%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with G-65%3B A-72 and Y-203. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B L-64%3B G-65%3B A-72%3B T-153%3B A-163%3B G-175 and Y-203. V->L;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:11387331,ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:8707053,ECO:0000269|PubMed:9759496,ECO:0000269|PubMed:9782051;Dbxref=PMID:11299042,PMID:11387331,PMID:12370172,PMID:8707053,PMID:9759496,PMID:9782051	
P42212	UniProtKB	Mutagenesis	68	68	.	.	.	Note=In R10-3%3B matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm%2C respectively%3B when associated with L-46%3B L-64%3B Q-162%3B A-163%3B V-167 and L-171. V->N;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18366185;Dbxref=PMID:18366185	
P42212	UniProtKB	Mutagenesis	69	69	.	.	.	Note=In P4%3B leads to no detectable fluorescence. In mut2.2%3B shifts fluorescence lifetime from 3.03 to 1.94 ns%3B when associated with R-30 and H-145. In mut3.3%3B shifts fluorescence lifetime from 3.03 to 1.88 ns%3B when associated with R-30%3B L-46 and H-145. Q->H;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:16201762,ECO:0000269|PubMed:7809066;Dbxref=PMID:16201762,PMID:7809066	
P42212	UniProtKB	Mutagenesis	69	69	.	.	.	Note=In RSGFP4%3B increases fluorescence and shifts the major exitation peak to 489-490 nm%3B when associated with M-64 and G-65. Q->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9634755;Dbxref=PMID:9634755	
P42212	UniProtKB	Mutagenesis	69	69	.	.	.	Note=In Citrinine%3B leads to excitation and emission peaks of 516 and 529 nm%2C respectively%3B when associated with L-68%3B A-72 and Y-203. Q->M;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:11387331;Dbxref=PMID:11387331	
P42212	UniProtKB	Mutagenesis	72	72	.	.	.	Note=Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In GFPmut 3%3B highly fluorescent mutant when excited at 488 nm%3B when associated with G-65. In EYFP%3B leads to yellow fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with G-65%3B L-68 and Y-203. In GFPmut 2%3B red-shifts by about 100 nm the excitation maxima%2C permitting efficient excitation at 488 nm and increases fluorescence%3B when associated with A-65 and L-68. In Citrinine%3B leads to excitation and emission peaks of 516 and 529 nm%2C respectively%3B when associated with L-68%3B M-69 and Y-203. In YFP 10C%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with G-65%3B L-68%3B and Y-203. In Topaz%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with G-65%3B R-79 and Y-203. In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B T-65%3B W-66%3B A-145%3B I-146%3B D-148%3B T-153 and A-163. In Sapphire/H9-40%3B exhibits a huge Stoke's shift%2C with an excitation peak at 399 nm and an emission peak at 511 nm%3B when associated with F-145 and I-203. In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B T-105%3B F-145%3B V-171%3B S-198 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. In Esmerald%3B leads to brighter fluorescence%3B when associated with T-65%3B K-149%3B T-153 and T-167. In VisGreen%3B leads to brighter fluorescence%3B when associated with L-64%3B T-65%3B K-149 and T-167. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B L-64%3B G-65%3B L-68%3B T-153%3B A-163%3B G-175 and Y-203. S->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:11387331,ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:12769828,ECO:0000269|PubMed:14990965,ECO:0000269|PubMed:17658219,ECO:0000269|PubMed:22891786,ECO:0000269|PubMed:8707053,ECO:0000269|PubMed:9145105,ECO:0000269|PubMed:9759496,ECO:0000269|PubMed:9782051;Dbxref=PMID:11299042,PMID:11387331,PMID:12370172,PMID:12769828,PMID:14990965,PMID:17658219,PMID:22891786,PMID:8707053,PMID:9145105,PMID:9759496,PMID:9782051	
P42212	UniProtKB	Mutagenesis	79	79	.	.	.	Note=In Topaz%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with G-65%3B A-72 and Y-203. K->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9759496;Dbxref=PMID:9759496	
P42212	UniProtKB	Mutagenesis	80	80	.	.	.	Note=In Azurite%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with H-66%3B F-145%3B I-150 and R-224. Q->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	99	99	.	.	.	Note=In alphaGFP/cycle 3 GFP%3B improves folding at 37 degrees Celsius%2C reduces aggregation at high concentrations%2C and increases the diffusibility of the protein inside cells%3B when associated with T-153 and A-163. F->S;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:9370472,ECO:0000269|PubMed:9630892,ECO:0000269|PubMed:9631088;Dbxref=PMID:9370472,PMID:9630892,PMID:9631088	
P42212	UniProtKB	Mutagenesis	103	103	.	.	.	Note=In mut1.27%3B shifts fluorescence lifetime from 3.03 to 2.85 ns%3B when associated with H-145. D->E;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16201762;Dbxref=PMID:16201762	
P42212	UniProtKB	Mutagenesis	105	105	.	.	.	Note=In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B F-145%3B V-171%3B S-198 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. N->T;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	128	128	.	.	.	Note=In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. I->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	145	145	.	.	.	Note=In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B T-65%3B W-66%3B A-72%3B I-146%3B D-148%3B T-153 and A-163. Y->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:14990965;Dbxref=PMID:14990965	
P42212	UniProtKB	Mutagenesis	145	145	.	.	.	Note=In mut1.9%3B shifts fluorescence lifetime from 3.03 to 2.74 ns. Y->C;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16201762;Dbxref=PMID:16201762	
P42212	UniProtKB	Mutagenesis	145	145	.	.	.	Note=In Sapphire/H9-40%3B exhibits a huge Stoke's shift%2C with an excitation peak at 399 nm and an emission peak at 511 nm%3B when associated with A-72 and I-203. In Azurite%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with H-66%3B R-80%3B I-150 and R-224. In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-171%3B S-198 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B I-150%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. Y->F;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12769828,ECO:0000269|PubMed:22891786;Dbxref=PMID:12769828,PMID:22891786	
P42212	UniProtKB	Mutagenesis	145	145	.	.	.	Note=In mut1.3%3B shifts fluorescence lifetime from 3.03 to 2.78 ns. In mut1.5%3B shifts fluorescence lifetime from 3.03 to 2.72 ns%3B when associated with A-193. In mut1.27%3B shifts fluorescence lifetime from 3.03 to 2.85 ns%3B when associated with E-103. In mut1.28%3B shifts fluorescence lifetime from 3.03 to 2.76 ns%3B when associated with R-30. In mut2.1%3B shifts fluorescence lifetime from 3.03 to 2.50 ns%3B when associated with A-176 and I-198. In mut2.2%3B shifts fluorescence lifetime from 3.03 to 1.94 ns%3B when associated with R-30 and H-69. In mut3.3%3B shifts fluorescence lifetime from 3.03 to 1.88 ns%3B when associated with R-30%3B L-46 and H-69. Y->H;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16201762;Dbxref=PMID:16201762	
P42212	UniProtKB	Mutagenesis	146	146	.	.	.	Note=In ECFP%3B leads to cyan fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with L-64%3B T-65%3B W-66%3B T-153 and A-163. In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B T-65%3B W-66%3B A-72%3B A-145%3B D-148%3B T-153 and A-163. N->I;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:14990965,ECO:0000269|PubMed:15065907;Dbxref=PMID:11299042,PMID:14990965,PMID:15065907	
P42212	UniProtKB	Mutagenesis	147	147	.	.	.	Note=Increases fluorescence at warmer temperatures such as 37 degrees Celsius. S->P;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:9125154;Dbxref=PMID:9125154	
P42212	UniProtKB	Mutagenesis	148	148	.	.	.	Note=In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B T-65%3B W-66%3B A-72%3B A-145%3B I-146%3B T-153 and A-163. H->D;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:14990965;Dbxref=PMID:14990965	
P42212	UniProtKB	Mutagenesis	149	149	.	.	.	Note=Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In Esmerald%3B leads to brighter fluorescence%3B when associated with T-65%3B A-72%3B T-153 and T-167. In VisGreen%3B leads to brighter fluorescence%3B when associated with L-64%3B T-65%3B A-72 and T-167. N->K;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17658219,ECO:0000269|PubMed:9145105;Dbxref=PMID:17658219,PMID:9145105	
P42212	UniProtKB	Mutagenesis	150	150	.	.	.	Note=In Azurite%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with H-66%3B R-80%3B F-145 and R-224. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B V-155%3B V-171%3B S-198%3B V-206 and V-224. V->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	153	153	.	.	.	Note=Increases fluorescence at warmer temperatures such as 37 degrees Celsius. In alphaGFP/cycle 3 GFP%3B improves folding at 37 degrees Celsius%2C reduces aggregation at high concentrations%2C and increases the diffusibility of the protein inside cells%3B when associated with S-99 and A-163. In ECFP%3B leads to cyan fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with L-64%3B T-65%3B W-66%3B I-146 and A-163. In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B T-65%3B W-66%3B A-72%3B A-145%3B I-146%3B D-148 and A-163. In Esmerald%3B leads to brighter fluorescence%3B when associated with T-65%3B A-72%3B K-149 and T-167. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B L-64%3B G-65%3B L-68%3B A-72%3B A-163%3B G-175 and Y-203. M->T;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:14990965,ECO:0000269|PubMed:15065907,ECO:0000269|PubMed:17658219,ECO:0000269|PubMed:9145105,ECO:0000269|PubMed:9370472,ECO:0000269|PubMed:9630892,ECO:0000269|PubMed:9631088;Dbxref=PMID:11299042,PMID:12370172,PMID:14990965,PMID:15065907,PMID:17658219,PMID:9145105,PMID:9370472,PMID:9630892,PMID:9631088	
P42212	UniProtKB	Mutagenesis	155	155	.	.	.	Note=In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-171%3B S-198%3B V-206 and V-224. D->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	162	162	.	.	.	Note=In R10-3%3B matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm%2C respectively%3B when associated with L-46%3B L-64%3B N-68%3B A-163%3B V-167 and L-171. K->Q;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18366185;Dbxref=PMID:18366185	
P42212	UniProtKB	Mutagenesis	163	163	.	.	.	Note=In GFPB%3B leads to enhanced fluorescence at 37 degrees Celsius. In GFPA%3B leads to even higher fluorescence at 37 degrees Celsius than GFPA%3B whenassociated with G-175. In alphaGFP/cycle 3 GFP%3B improves folding at 37 degrees Celsius%2C reduces aggregation at high concentrations%2C and increases the diffusibility of the protein inside cells%3B when associated with S-99 and T-153. In R10-3%3B matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm%2C respectively%3B when associated with L-46%3B L-64%3B N-68%3B Q-162%3B V-167 and L-171. In ECFP%3B leads to cyan fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with L-64%3B T-65%3B W-66%3B I-146 and T-153. In Cerulean%3B leads to improved quantum yield%2C a higher extinction coefficient and is 2.5-fold brighter than ECFP%3B when associated with L-64%3B T-65%3B W-66%3B A-72%3B A-145%3B I-146%3B D-148 and T-153. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B L-64%3B G-65%3B L-68%3B A-72%3B T-153%3B G-175 and Y-203. V->A;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:14990965,ECO:0000269|PubMed:15065907,ECO:0000269|PubMed:18366185,ECO:0000269|PubMed:8994830,ECO:0000269|PubMed:9370472,ECO:0000269|PubMed:9630892,ECO:0000269|PubMed:9631088;Dbxref=PMID:11299042,PMID:12370172,PMID:14990965,PMID:15065907,PMID:18366185,PMID:8994830,PMID:9370472,PMID:9630892,PMID:9631088	
P42212	UniProtKB	Mutagenesis	167	167	.	.	.	Note=In P11%3B increases fluorescence at 475 nm excitation and at warmer temperatures such as 37 degrees Celsius. In Esmerald%3B leads to brighter fluorescence%3B when associated with T-65%3B A-72%3B K-149 and T-153. In VisGreen%3B leads to brighter fluorescence%3B when associated with L-64%3B T-65%3B A-72 and K-149. I->T;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:17658219,ECO:0000269|PubMed:7809066,ECO:0000269|PubMed:9145105;Dbxref=PMID:17658219,PMID:7809066,PMID:9145105	
P42212	UniProtKB	Mutagenesis	167	167	.	.	.	Note=In P9%3B increases fluorescence at 475 nm excitation. In R10-3%3B matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm%2C respectively%3B when associated with L-46%3B L-64%3B N-68%3B Q-162%3B A-163 and L-171. I->V;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:18366185,ECO:0000269|PubMed:7809066;Dbxref=PMID:18366185,PMID:7809066	
P42212	UniProtKB	Mutagenesis	171	171	.	.	.	Note=In R10-3%3B matures to the red-emitting state with excitation and emission maxima at 555 and 585 nm%2C respectively%3B when associated with L-46%3B L-64%3B N-68%3B Q-162%3B A-163 and V-167. I->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:18366185;Dbxref=PMID:18366185	
P42212	UniProtKB	Mutagenesis	171	171	.	.	.	Note=In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B F-145%3B S-198 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B S-198%3B V-206 and V-224. I->V;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	175	175	.	.	.	Note=In GFPA%3B leads to enhanced fluorescence at 37 degrees Celsius%3B when associated with A-163. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B L-64%3B G-65%3B L-68%3B A-72%3B T-153%3B A-163 and Y-203. S->G;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:8994830;Dbxref=PMID:12370172,PMID:8994830	
P42212	UniProtKB	Mutagenesis	176	176	.	.	.	Note=In mut2.1%3B shifts fluorescence lifetime from 3.03 to 2.50 ns%3B when associated with H-145 and I-198. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16201762;Dbxref=PMID:16201762	
P42212	UniProtKB	Mutagenesis	193	193	.	.	.	Note=In mut1.5%3B shifts fluorescence lifetime from 3.03 to 2.72 ns%3B when associated with H-145. V->A;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16201762;Dbxref=PMID:16201762	
P42212	UniProtKB	Mutagenesis	198	198	.	.	.	Note=In mut2.1%3B shifts fluorescence lifetime from 3.03 to 2.50 ns%3B when associated with H-145 and A-176. N->I;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16201762;Dbxref=PMID:16201762	
P42212	UniProtKB	Mutagenesis	198	198	.	.	.	Note=In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B F-145%3B V-171 and V-206. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198 and V-224. N->S;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Mutagenesis	202	202	.	.	.	Note=In H9%3B increases fluorescence at 395 nm excitation%3B when associated with I-203. S->F;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7809066;Dbxref=PMID:7809066	
P42212	UniProtKB	Mutagenesis	203	203	.	.	.	Note=Results in significantly red-shifted excitation and emission maxima. T->H%2CW%2CF;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8703075;Dbxref=PMID:8703075	
P42212	UniProtKB	Mutagenesis	203	203	.	.	.	Note=Suppresses the 475 nm excitation peak%2C leaving only the shorter wavelength peak at 399 nm. In H9%3B increases fluorescence at 395 nm excitation%3B when associated with I-203. In Sapphire/H9-40%3B exhibits a huge Stoke's shift%2C with an excitation peak at 399 nm and an emission peak at 511 nm%3B when associated with A-72 and F-145. T->I;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:12769828,ECO:0000269|PubMed:7796912,ECO:0000269|PubMed:7809066;Dbxref=PMID:12769828,PMID:7796912,PMID:7809066	
P42212	UniProtKB	Mutagenesis	203	203	.	.	.	Note=Gives rise to yellow-emission variants. In EYFP%3B leads to yellow fluorescence%2C folds faster and more efficiently at 37 degrees Celsius and has superior solubility and brightness%3B when associated with G-65%3B L-68 and A-72. In Citrinine%3B leads to excitation and emission peaks of 516 and 529 nm%2C respectively%3B when associated with L-68%3B M-69 and A-72. In YFP 10C%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with G-65%3B L-68 and A-72. In Topaz%3B shifts the major emission and exitation peak up to 20 nm%3B when associated with G-65%3B A-72 and R-79. In Venus%3B leads to yellow fluorescence%2C improved maturation and reduced environmental sensitivity%3B when associated with L-46%3B L-64%3B G-65%3B L-68%3B A-72%3B T-153%3B A-163 and G-175. T->Y;Ontology_term=ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:11299042,ECO:0000269|PubMed:11387331,ECO:0000269|PubMed:12370172,ECO:0000269|PubMed:9759496,ECO:0000269|PubMed:9782051;Dbxref=PMID:11299042,PMID:11387331,PMID:12370172,PMID:9759496,PMID:9782051	
P42212	UniProtKB	Mutagenesis	206	206	.	.	.	Note=Abolishes the tendency to dimerize and leads to monomeric fluorescent proteins. A->K;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:16739159;Dbxref=PMID:16739159	
P42212	UniProtKB	Mutagenesis	206	206	.	.	.	Note=In EBFP1.2%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B F-145%3B V-171 and S-198. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198 and V-206. A->V	
P42212	UniProtKB	Mutagenesis	222	222	.	.	.	Note=Suppresses the 399 nm excitation peak%2C leaving only the longer wavelength peak at 475 nm. E->G;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:7796912;Dbxref=PMID:7796912	
P42212	UniProtKB	Mutagenesis	224	224	.	.	.	Note=In Azurite%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with H-66%3B R-80%3B F-145 and I-150. In EBFP2.0%3B shifts the excitation and emission spectra to shorter wavelengths and increases quantum yields compared to BFP%3B when associated with R-30%3B N-39%3B H-66%3B A-72%3B T-105%3B V-128%3B F-145%3B I-150%3B V-155%3B V-171%3B S-198 and V-206. V->R;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:22891786;Dbxref=PMID:22891786	
P42212	UniProtKB	Sequence conflict	2	2	.	.	.	Note=S->G;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P42212	UniProtKB	Sequence conflict	25	25	.	.	.	Note=H->Q;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P42212	UniProtKB	Sequence conflict	80	80	.	.	.	Note=Q->R;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P42212	UniProtKB	Sequence conflict	157	157	.	.	.	Note=Q->P;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P42212	UniProtKB	Sequence conflict	172	172	.	.	.	Note=E->K;Ontology_term=ECO:0000305;evidence=ECO:0000305	
P42212	UniProtKB	Turn	1	3	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6UHQ	
P42212	UniProtKB	Helix	5	8	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	12	22	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	25	36	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Helix	37	39	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	41	48	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	50	52	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:5LEM	
P42212	UniProtKB	Helix	57	60	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Helix	61	64	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Turn	65	67	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1QYO	
P42212	UniProtKB	Helix	69	71	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Helix	72	74	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6OA8	
P42212	UniProtKB	Helix	76	81	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Helix	83	86	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Turn	87	90	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	92	100	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	101	103	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6MWQ	
P42212	UniProtKB	Beta strand	105	115	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	118	129	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	132	134	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:1EMK	
P42212	UniProtKB	Turn	135	139	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	141	147	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4ZF5	
P42212	UniProtKB	Beta strand	148	154	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	158	171	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Turn	172	174	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4OGS	
P42212	UniProtKB	Beta strand	176	191	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Beta strand	198	208	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Helix	209	213	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4IK8	
P42212	UniProtKB	Beta strand	215	228	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6L27	
P42212	UniProtKB	Helix	233	236	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4GF6