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Protein

Green fluorescent protein

Gene

GFP

Organism
Aequorea victoria (Jellyfish)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca2+-activated photoprotein aequorin.

Absorptioni

Abs(max)=395 nm

Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.

GO - Biological processi

  • bioluminescence Source: UniProtKB
  • generation of precursor metabolites and energy Source: UniProtKB
  • protein-chromophore linkage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Photoprotein

Keywords - Biological processi

Luminescence

Keywords - Ligandi

Chromophore

Names & Taxonomyi

Protein namesi
Recommended name:
Green fluorescent protein
Gene namesi
Name:GFP
OrganismiAequorea victoria (Jellyfish)
Taxonomic identifieri6100 [NCBI]
Taxonomic lineageiEukaryotaMetazoaCnidariaHydrozoaHydroidolinaLeptothecataAequoreidaeAequorea

Pathology & Biotechi

Biotechnological usei

Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.1 Publication
Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi65S → G in mut3; highly fluorescent mutant when excited at 488 nm; when associated with A-72. 1 Publication1
Mutagenesisi65S → T: Increases fluoresence, photostability and shift the major exitation peak to 488 nm. 1 Publication1
Mutagenesisi72S → A in mut3; highly fluorescent mutant when excited at 488 nm; when associated with G-65. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001925761 – 238Green fluorescent proteinAdd BLAST238

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki65 ↔ 675-imidazolinone (Ser-Gly)
Modified residuei66(Z)-2,3-didehydrotyrosine1

Post-translational modificationi

Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.

Proteomic databases

PRIDEiP42212.

Expressioni

Tissue specificityi

Photocytes.

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

IntActiP42212. 1 interactor.

Structurei

Secondary structure

1238
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 8Combined sources5
Beta strandi12 – 22Combined sources11
Beta strandi25 – 36Combined sources12
Helixi37 – 39Combined sources3
Beta strandi41 – 48Combined sources8
Helixi57 – 60Combined sources4
Turni61 – 63Combined sources3
Turni65 – 67Combined sources3
Helixi69 – 71Combined sources3
Helixi76 – 81Combined sources6
Helixi83 – 86Combined sources4
Turni87 – 90Combined sources4
Beta strandi92 – 100Combined sources9
Beta strandi105 – 115Combined sources11
Beta strandi118 – 128Combined sources11
Beta strandi132 – 134Combined sources3
Turni135 – 139Combined sources5
Beta strandi141 – 147Combined sources7
Beta strandi149 – 155Combined sources7
Helixi156 – 158Combined sources3
Beta strandi160 – 171Combined sources12
Turni172 – 174Combined sources3
Beta strandi176 – 191Combined sources16
Beta strandi198 – 208Combined sources11
Beta strandi217 – 227Combined sources11
Helixi233 – 236Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B9CX-ray2.40A/B/C/D1-238[»]
1BFPX-ray2.10A1-238[»]
1C4FX-ray2.25A1-238[»]
1CV7X-ray2.50A1-228[»]
1EMAX-ray1.90A1-238[»]
1EMBX-ray2.13A1-238[»]
1EMCX-ray2.30A/B/C/D2-237[»]
1EMEX-ray2.50A2-237[»]
1EMFX-ray2.40A2-238[»]
1EMGX-ray2.00A1-229[»]
1EMKX-ray2.10A2-237[»]
1EMLX-ray2.30A2-237[»]
1EMMX-ray2.30A2-238[»]
1F09X-ray2.14A1-238[»]
1F0BX-ray2.10A1-238[»]
1GFLX-ray1.90A/B2-238[»]
1H6RX-ray1.50A/B/C1-238[»]
1HCJX-ray1.80A/B/C/D1-238[»]
1HUYX-ray2.20A2-238[»]
1JBYX-ray1.80A1-238[»]
1JBZX-ray1.50A1-238[»]
1JC0X-ray2.00A/B/C1-238[»]
1JC1X-ray1.90A/B/C1-238[»]
1KP5X-ray2.60A/B1-238[»]
1KYPX-ray1.35A2-238[»]
1KYRX-ray1.50A2-238[»]
1KYSX-ray1.44A2-238[»]
1MYWX-ray2.20A2-238[»]
1Q4AX-ray1.45A1-238[»]
1Q4BX-ray1.48A1-238[»]
1Q4CX-ray1.55A1-238[»]
1Q4DX-ray1.58A1-238[»]
1Q4EX-ray1.38A1-238[»]
1Q73X-ray1.60A1-238[»]
1QXTX-ray2.00A2-229[»]
1QY3X-ray2.00A1-229[»]
1QYFX-ray1.50A1-227[»]
1QYOX-ray1.80A1-238[»]
1QYQX-ray1.80A2-238[»]
1RM9X-ray2.90A2-236[»]
1RMMX-ray1.90A2-227[»]
1RMOX-ray1.80A2-236[»]
1RMPX-ray3.00A2-229[»]
1RRXX-ray2.10A2-227[»]
1S6ZX-ray1.50A1-238[»]
1W7SX-ray1.85A/B/C/D1-238[»]
1W7TX-ray1.85A/B/C/D1-238[»]
1W7UX-ray1.85A/B/C/D1-238[»]
1YFPX-ray2.50A/B3-229[»]
1YHGX-ray2.50A/B2-238[»]
1YHHX-ray1.50A2-238[»]
1YHIX-ray1.90A2-238[»]
1YJ2X-ray1.50A2-238[»]
1YJFX-ray1.35A2-238[»]
1Z1PX-ray2.00A1-238[»]
1Z1QX-ray1.50A1-238[»]
2AH8X-ray2.24A/B1-238[»]
2AHAX-ray1.98A/B1-238[»]
2AWJX-ray1.60A2-229[»]
2AWKX-ray1.15A1-229[»]
2AWLX-ray1.85A1-229[»]
2AWMX-ray1.70A1-229[»]
2B3PX-ray1.40A1-238[»]
2B3QX-ray2.30A/B/C/D1-238[»]
2DUEX-ray1.24A1-238[»]
2DUFX-ray1.50A1-238[»]
2DUGX-ray1.40A1-238[»]
2DUHX-ray1.20A1-238[»]
2DUIX-ray1.36A1-238[»]
2EMDX-ray2.00A1-238[»]
2EMNX-ray2.30A1-238[»]
2EMOX-ray2.60A1-238[»]
2FWQX-ray1.40A2-238[»]
2FZUX-ray1.25A2-238[»]
2G16X-ray2.00A2-64[»]
B67-238[»]
2G2SX-ray1.20A2-63[»]
B66-238[»]
2G3DX-ray1.35A2-63[»]
B66-238[»]
2G5ZX-ray1.80A2-65[»]
B66-238[»]
2G6EX-ray1.30A2-238[»]
2H6VX-ray1.47A2-238[»]
2H9WX-ray1.82A2-237[»]
2HCGX-ray1.35A2-238[»]
2HFCX-ray1.20A2-238[»]
2HGDX-ray1.60A2-238[»]
2HGYX-ray2.05A2-238[»]
2HJOX-ray1.25A1-238[»]
2HQZX-ray1.20A1-238[»]
2HRSX-ray1.40A1-238[»]
2JADX-ray2.70A1-238[»]
2O24X-ray1.45A2-238[»]
2O29X-ray1.80A2-238[»]
2O2BX-ray1.94A2-238[»]
2OKWX-ray1.90A/B/C/D/E/F1-238[»]
2OKYX-ray2.40A/B1-238[»]
2Q57X-ray2.00A1-238[»]
2Q6PX-ray2.10A1-238[»]
2QRFX-ray1.50A1-230[»]
2QT2X-ray1.31A1-238[»]
2QU1X-ray1.70A1-238[»]
2QZ0X-ray1.20A2-229[»]
2WSNX-ray1.37A2-238[»]
2WSOX-ray1.15A2-238[»]
2WURX-ray0.90A1-237[»]
2Y0GX-ray1.50A2-238[»]
2YDZX-ray1.59A2-238[»]
2YE0X-ray1.47A2-238[»]
2YE1X-ray1.63A2-238[»]
2YFPX-ray2.60A1-238[»]
3CB9X-ray1.31A2-238[»]
3CBEX-ray1.49A2-238[»]
3CD1X-ray1.31A2-238[»]
3CD9X-ray1.50A2-238[»]
3DPWX-ray1.59A2-238[»]
3DPXX-ray1.50A2-238[»]
3DPZX-ray1.70A2-238[»]
3DQ1X-ray1.70A2-238[»]
3DQ2X-ray1.60A2-238[»]
3DQ3X-ray1.70A2-238[»]
3DQ4X-ray1.47A2-238[»]
3DQ5X-ray1.50A2-238[»]
3DQ6X-ray1.60A2-238[»]
3DQ7X-ray1.23A2-238[»]
3DQ8X-ray1.51A2-238[»]
3DQ9X-ray1.40A2-238[»]
3DQAX-ray1.44A2-238[»]
3DQCX-ray1.49A2-238[»]
3DQDX-ray1.40A2-238[»]
3DQEX-ray1.43A2-238[»]
3DQFX-ray1.46A2-238[»]
3DQHX-ray1.45A2-238[»]
3DQIX-ray1.42A2-238[»]
3DQJX-ray1.51A2-238[»]
3DQKX-ray1.40A2-238[»]
3DQLX-ray1.47A2-238[»]
3DQMX-ray1.44A2-238[»]
3DQNX-ray1.44A2-238[»]
3DQOX-ray1.50A2-238[»]
3DQUX-ray1.42A2-238[»]
3ED8X-ray2.70A/B/C/D/E2-238[»]
3EK4X-ray2.65A2-238[»]
3EK7X-ray1.85A2-238[»]
3EK8X-ray2.80A2-238[»]
3EKHX-ray2.00A2-238[»]
3EKJX-ray2.80A2-238[»]
3EVPX-ray1.45A2-144[»]
3EVRX-ray2.00A2-144[»]
3EVUX-ray1.75A2-144[»]
3EVVX-ray2.60A2-238[»]
3G9AX-ray1.61A1-238[»]
3GEXX-ray1.60A1-238[»]
3GJ1X-ray1.80A/B/C/D1-230[»]
3GJ2X-ray1.90A/B/C/D1-230[»]
3I19X-ray1.36A1-238[»]
3K1KX-ray2.15A/B1-238[»]
3LA1X-ray1.29A1-238[»]
3O77X-ray2.35A2-238[»]
3O78X-ray2.60A/B2-237[»]
3OGOX-ray2.80A/B/C/D1-238[»]
3OSQX-ray1.90A2-238[»]
3OSRX-ray2.00A/B2-238[»]
3P28X-ray1.80A3-229[»]
3SG2X-ray2.00A2-238[»]
3SG3X-ray2.10A2-238[»]
3SG4X-ray2.40A2-238[»]
3SG5X-ray1.90A2-238[»]
3SG6X-ray1.70A2-238[»]
3SG7X-ray1.90A2-238[»]
3SRYX-ray1.16A2-238[»]
3SS0X-ray1.49A2-238[»]
3SSHX-ray1.28A2-238[»]
3SSKX-ray1.36A2-238[»]
3SSLX-ray1.45A2-238[»]
3SSPX-ray1.63A2-238[»]
3SSTX-ray1.40A2-238[»]
3SSVX-ray1.86A2-238[»]
3SSYX-ray1.77A2-238[»]
3ST0X-ray1.19A2-238[»]
3SV5X-ray1.53A2-238[»]
3SVBX-ray1.30A2-238[»]
3SVCX-ray1.31A2-238[»]
3SVDX-ray1.78A2-238[»]
3SVEX-ray1.49A2-238[»]
3U8PX-ray2.75A/B/C2-238[»]
3UFZX-ray1.85A2-229[»]
3UG0X-ray2.09A2-229[»]
3V3DX-ray1.95A2-238[»]
3VHTX-ray2.40A1-230[»]
3W1CX-ray1.30A2-238[»]
3W1DX-ray1.50A2-238[»]
3WLCX-ray2.49A2-238[»]
3WLDX-ray2.70A2-144[»]
3ZTFX-ray1.31A2-238[»]
4ANJX-ray2.60A1-238[»]
4AR7X-ray1.23A2-238[»]
4AS8X-ray1.02A2-238[»]
4B5YX-ray1.45A2-238[»]
4BDUX-ray3.00A/B/C/D1-230[»]
4EN1X-ray1.62A/B2-238[»]
4EULX-ray1.35A2-238[»]
4GESX-ray1.23B1-238[»]
4GF6X-ray1.10B1-237[»]
4H47X-ray1.90A1-238[»]
4H48X-ray1.45A1-238[»]
4IK1X-ray2.00A2-144[»]
4IK3X-ray2.01A149-238[»]
A2-142[»]
4IK4X-ray2.01A2-142[»]
A149-238[»]
4IK5X-ray2.50A2-142[»]
A149-238[»]
4IK8X-ray1.55A2-144[»]
A149-238[»]
4IK9X-ray1.80A2-144[»]
4J88X-ray2.08A/B2-238[»]
4J89X-ray2.10A/B2-238[»]
4J8AX-ray1.26A2-238[»]
4JFGX-ray3.00A/B/C/D/E/F/G/H1-238[»]
4JRBX-ray2.41A1-229[»]
4KA9X-ray1.58A5-238[»]
4KAGX-ray1.12A1-238[»]
4KEXX-ray1.60A1-238[»]
4KF5X-ray2.60A/B1-196[»]
4KW4X-ray1.75A2-238[»]
4KW8X-ray2.46A2-238[»]
4KW9X-ray1.80A2-238[»]
4L12X-ray1.78A2-230[»]
4L13X-ray1.66A2-230[»]
4L1IX-ray1.20A2-230[»]
4LQTX-ray1.10A2-238[»]
4LQUX-ray1.60A/B/C/D2-238[»]
4LW5X-ray2.55A/B/C/D/E2-238[»]
4N3DX-ray1.34A/B1-230[»]
4NDJX-ray1.85A2-238[»]
4NDKX-ray2.30A/B2-238[»]
4OGSX-ray2.21A/B1-238[»]
4ORNX-ray1.71A/B2-238[»]
4P1QX-ray1.50A3-231[»]
4P7HX-ray3.20A/B5-238[»]
4PA0X-ray2.25A/B5-234[»]
4PFEX-ray2.60A/B2-229[»]
4U2VX-ray2.30A/B/C/D1-230[»]
4XBIX-ray2.01A/B2-230[»]
4XGYX-ray1.49A2-238[»]
4XL5X-ray2.00A2-238[»]
4XOVX-ray1.20A2-238[»]
4XOWX-ray1.25A2-238[»]
4XVPX-ray3.40A/B/C2-238[»]
4Z4KX-ray2.80A/B1-230[»]
4Z4MX-ray2.15A/B1-230[»]
4ZF3X-ray1.90A/B51-236[»]
4ZF4X-ray1.82A/B4-237[»]
4ZF5X-ray1.70A/B4-237[»]
5AQBX-ray1.37B2-231[»]
5DPGX-ray1.85A2-238[»]
5DPHX-ray1.42A/B2-238[»]
5DPIX-ray2.54A/B/C/D/E/F2-238[»]
5DPJX-ray2.50A/B/C/D2-238[»]
5DQBX-ray1.25A2-238[»]
5DQMX-ray1.30A2-238[»]
5DRFX-ray1.14A2-238[»]
5DRGX-ray1.14A2-238[»]
5DTXX-ray1.45A1-63[»]
A65-238[»]
5DTYX-ray1.50A1-63[»]
A65-238[»]
5DTZX-ray1.50A/B/C/D2-238[»]
5DU0X-ray2.35A/B/C/D2-238[»]
5EHUX-ray1.45A/B2-238[»]
5F9GX-ray2.77A2-144[»]
5FGUX-ray1.90A1-229[»]
5HZOX-ray2.49A/B1-235[»]
5J2OX-ray1.50A2-238[»]
5KTGX-ray2.80A/B1-230[»]
ProteinModelPortaliP42212.
SMRiP42212.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42212.

Family & Domainsi

Sequence similaritiesi

Belongs to the GFP family.Curated

Family and domain databases

Gene3Di2.40.155.10. 1 hit.
InterProiIPR009017. GFP.
IPR023413. GFP-like.
IPR011584. GFP-related.
IPR000786. Green_fluorescent_prot.
[Graphical view]
PfamiPF01353. GFP. 1 hit.
[Graphical view]
PRINTSiPR01229. GFLUORESCENT.
SUPFAMiSSF54511. SSF54511. 1 hit.

Sequencei

Sequence statusi: Complete.

P42212-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKGEELFTG VVPILVELDG DVNGHKFSVS GEGEGDATYG KLTLKFICTT
60 70 80 90 100
GKLPVPWPTL VTTFSYGVQC FSRYPDHMKQ HDFFKSAMPE GYVQERTIFF
110 120 130 140 150
KDDGNYKTRA EVKFEGDTLV NRIELKGIDF KEDGNILGHK LEYNYNSHNV
160 170 180 190 200
YIMADKQKNG IKVNFKIRHN IEDGSVQLAD HYQQNTPIGD GPVLLPDNHY
210 220 230
LSTQSALSKD PNEKRDHMVL LEFVTAAGIT HGMDELYK
Length:238
Mass (Da):26,886
Last modified:November 1, 1995 - v1
Checksum:iEA5A6F21FBFB6E05
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2S → G in CAA65278 (PubMed:9154981).Curated1
Sequence conflicti25H → Q in CAA65278 (PubMed:9154981).Curated1
Sequence conflicti80Q → R in CAA65278 (PubMed:9154981).Curated1
Sequence conflicti157Q → P in AAA58246 (PubMed:8137953).Curated1
Sequence conflicti172E → K in AAA58246 (PubMed:8137953).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti100F → Y.1 Publication1
Natural varianti108T → S.1 Publication1
Natural varianti141L → M.1 Publication1
Natural varianti219V → I.1 Publication1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62654 mRNA. Translation: AAA27722.1.
M62653 mRNA. Translation: AAA27721.1.
L29345 mRNA. Translation: AAA58246.1.
X96418 mRNA. Translation: CAA65278.1.
U73901 Genomic DNA. Translation: AAB18957.1.
PIRiJS0692. JQ1514.

Cross-referencesi

Web resourcesi

Protein Spotlight

The greenest of us all - Issue 11 of June 2001

Protein Spotlight

Paint my thoughts - Issue 108 of August 2009

Wikipedia

Green fluorescent protein entry

Protein Spotlight

Paint my thoughts - Issue 108 of November 2007

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M62654 mRNA. Translation: AAA27722.1.
M62653 mRNA. Translation: AAA27721.1.
L29345 mRNA. Translation: AAA58246.1.
X96418 mRNA. Translation: CAA65278.1.
U73901 Genomic DNA. Translation: AAB18957.1.
PIRiJS0692. JQ1514.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1B9CX-ray2.40A/B/C/D1-238[»]
1BFPX-ray2.10A1-238[»]
1C4FX-ray2.25A1-238[»]
1CV7X-ray2.50A1-228[»]
1EMAX-ray1.90A1-238[»]
1EMBX-ray2.13A1-238[»]
1EMCX-ray2.30A/B/C/D2-237[»]
1EMEX-ray2.50A2-237[»]
1EMFX-ray2.40A2-238[»]
1EMGX-ray2.00A1-229[»]
1EMKX-ray2.10A2-237[»]
1EMLX-ray2.30A2-237[»]
1EMMX-ray2.30A2-238[»]
1F09X-ray2.14A1-238[»]
1F0BX-ray2.10A1-238[»]
1GFLX-ray1.90A/B2-238[»]
1H6RX-ray1.50A/B/C1-238[»]
1HCJX-ray1.80A/B/C/D1-238[»]
1HUYX-ray2.20A2-238[»]
1JBYX-ray1.80A1-238[»]
1JBZX-ray1.50A1-238[»]
1JC0X-ray2.00A/B/C1-238[»]
1JC1X-ray1.90A/B/C1-238[»]
1KP5X-ray2.60A/B1-238[»]
1KYPX-ray1.35A2-238[»]
1KYRX-ray1.50A2-238[»]
1KYSX-ray1.44A2-238[»]
1MYWX-ray2.20A2-238[»]
1Q4AX-ray1.45A1-238[»]
1Q4BX-ray1.48A1-238[»]
1Q4CX-ray1.55A1-238[»]
1Q4DX-ray1.58A1-238[»]
1Q4EX-ray1.38A1-238[»]
1Q73X-ray1.60A1-238[»]
1QXTX-ray2.00A2-229[»]
1QY3X-ray2.00A1-229[»]
1QYFX-ray1.50A1-227[»]
1QYOX-ray1.80A1-238[»]
1QYQX-ray1.80A2-238[»]
1RM9X-ray2.90A2-236[»]
1RMMX-ray1.90A2-227[»]
1RMOX-ray1.80A2-236[»]
1RMPX-ray3.00A2-229[»]
1RRXX-ray2.10A2-227[»]
1S6ZX-ray1.50A1-238[»]
1W7SX-ray1.85A/B/C/D1-238[»]
1W7TX-ray1.85A/B/C/D1-238[»]
1W7UX-ray1.85A/B/C/D1-238[»]
1YFPX-ray2.50A/B3-229[»]
1YHGX-ray2.50A/B2-238[»]
1YHHX-ray1.50A2-238[»]
1YHIX-ray1.90A2-238[»]
1YJ2X-ray1.50A2-238[»]
1YJFX-ray1.35A2-238[»]
1Z1PX-ray2.00A1-238[»]
1Z1QX-ray1.50A1-238[»]
2AH8X-ray2.24A/B1-238[»]
2AHAX-ray1.98A/B1-238[»]
2AWJX-ray1.60A2-229[»]
2AWKX-ray1.15A1-229[»]
2AWLX-ray1.85A1-229[»]
2AWMX-ray1.70A1-229[»]
2B3PX-ray1.40A1-238[»]
2B3QX-ray2.30A/B/C/D1-238[»]
2DUEX-ray1.24A1-238[»]
2DUFX-ray1.50A1-238[»]
2DUGX-ray1.40A1-238[»]
2DUHX-ray1.20A1-238[»]
2DUIX-ray1.36A1-238[»]
2EMDX-ray2.00A1-238[»]
2EMNX-ray2.30A1-238[»]
2EMOX-ray2.60A1-238[»]
2FWQX-ray1.40A2-238[»]
2FZUX-ray1.25A2-238[»]
2G16X-ray2.00A2-64[»]
B67-238[»]
2G2SX-ray1.20A2-63[»]
B66-238[»]
2G3DX-ray1.35A2-63[»]
B66-238[»]
2G5ZX-ray1.80A2-65[»]
B66-238[»]
2G6EX-ray1.30A2-238[»]
2H6VX-ray1.47A2-238[»]
2H9WX-ray1.82A2-237[»]
2HCGX-ray1.35A2-238[»]
2HFCX-ray1.20A2-238[»]
2HGDX-ray1.60A2-238[»]
2HGYX-ray2.05A2-238[»]
2HJOX-ray1.25A1-238[»]
2HQZX-ray1.20A1-238[»]
2HRSX-ray1.40A1-238[»]
2JADX-ray2.70A1-238[»]
2O24X-ray1.45A2-238[»]
2O29X-ray1.80A2-238[»]
2O2BX-ray1.94A2-238[»]
2OKWX-ray1.90A/B/C/D/E/F1-238[»]
2OKYX-ray2.40A/B1-238[»]
2Q57X-ray2.00A1-238[»]
2Q6PX-ray2.10A1-238[»]
2QRFX-ray1.50A1-230[»]
2QT2X-ray1.31A1-238[»]
2QU1X-ray1.70A1-238[»]
2QZ0X-ray1.20A2-229[»]
2WSNX-ray1.37A2-238[»]
2WSOX-ray1.15A2-238[»]
2WURX-ray0.90A1-237[»]
2Y0GX-ray1.50A2-238[»]
2YDZX-ray1.59A2-238[»]
2YE0X-ray1.47A2-238[»]
2YE1X-ray1.63A2-238[»]
2YFPX-ray2.60A1-238[»]
3CB9X-ray1.31A2-238[»]
3CBEX-ray1.49A2-238[»]
3CD1X-ray1.31A2-238[»]
3CD9X-ray1.50A2-238[»]
3DPWX-ray1.59A2-238[»]
3DPXX-ray1.50A2-238[»]
3DPZX-ray1.70A2-238[»]
3DQ1X-ray1.70A2-238[»]
3DQ2X-ray1.60A2-238[»]
3DQ3X-ray1.70A2-238[»]
3DQ4X-ray1.47A2-238[»]
3DQ5X-ray1.50A2-238[»]
3DQ6X-ray1.60A2-238[»]
3DQ7X-ray1.23A2-238[»]
3DQ8X-ray1.51A2-238[»]
3DQ9X-ray1.40A2-238[»]
3DQAX-ray1.44A2-238[»]
3DQCX-ray1.49A2-238[»]
3DQDX-ray1.40A2-238[»]
3DQEX-ray1.43A2-238[»]
3DQFX-ray1.46A2-238[»]
3DQHX-ray1.45A2-238[»]
3DQIX-ray1.42A2-238[»]
3DQJX-ray1.51A2-238[»]
3DQKX-ray1.40A2-238[»]
3DQLX-ray1.47A2-238[»]
3DQMX-ray1.44A2-238[»]
3DQNX-ray1.44A2-238[»]
3DQOX-ray1.50A2-238[»]
3DQUX-ray1.42A2-238[»]
3ED8X-ray2.70A/B/C/D/E2-238[»]
3EK4X-ray2.65A2-238[»]
3EK7X-ray1.85A2-238[»]
3EK8X-ray2.80A2-238[»]
3EKHX-ray2.00A2-238[»]
3EKJX-ray2.80A2-238[»]
3EVPX-ray1.45A2-144[»]
3EVRX-ray2.00A2-144[»]
3EVUX-ray1.75A2-144[»]
3EVVX-ray2.60A2-238[»]
3G9AX-ray1.61A1-238[»]
3GEXX-ray1.60A1-238[»]
3GJ1X-ray1.80A/B/C/D1-230[»]
3GJ2X-ray1.90A/B/C/D1-230[»]
3I19X-ray1.36A1-238[»]
3K1KX-ray2.15A/B1-238[»]
3LA1X-ray1.29A1-238[»]
3O77X-ray2.35A2-238[»]
3O78X-ray2.60A/B2-237[»]
3OGOX-ray2.80A/B/C/D1-238[»]
3OSQX-ray1.90A2-238[»]
3OSRX-ray2.00A/B2-238[»]
3P28X-ray1.80A3-229[»]
3SG2X-ray2.00A2-238[»]
3SG3X-ray2.10A2-238[»]
3SG4X-ray2.40A2-238[»]
3SG5X-ray1.90A2-238[»]
3SG6X-ray1.70A2-238[»]
3SG7X-ray1.90A2-238[»]
3SRYX-ray1.16A2-238[»]
3SS0X-ray1.49A2-238[»]
3SSHX-ray1.28A2-238[»]
3SSKX-ray1.36A2-238[»]
3SSLX-ray1.45A2-238[»]
3SSPX-ray1.63A2-238[»]
3SSTX-ray1.40A2-238[»]
3SSVX-ray1.86A2-238[»]
3SSYX-ray1.77A2-238[»]
3ST0X-ray1.19A2-238[»]
3SV5X-ray1.53A2-238[»]
3SVBX-ray1.30A2-238[»]
3SVCX-ray1.31A2-238[»]
3SVDX-ray1.78A2-238[»]
3SVEX-ray1.49A2-238[»]
3U8PX-ray2.75A/B/C2-238[»]
3UFZX-ray1.85A2-229[»]
3UG0X-ray2.09A2-229[»]
3V3DX-ray1.95A2-238[»]
3VHTX-ray2.40A1-230[»]
3W1CX-ray1.30A2-238[»]
3W1DX-ray1.50A2-238[»]
3WLCX-ray2.49A2-238[»]
3WLDX-ray2.70A2-144[»]
3ZTFX-ray1.31A2-238[»]
4ANJX-ray2.60A1-238[»]
4AR7X-ray1.23A2-238[»]
4AS8X-ray1.02A2-238[»]
4B5YX-ray1.45A2-238[»]
4BDUX-ray3.00A/B/C/D1-230[»]
4EN1X-ray1.62A/B2-238[»]
4EULX-ray1.35A2-238[»]
4GESX-ray1.23B1-238[»]
4GF6X-ray1.10B1-237[»]
4H47X-ray1.90A1-238[»]
4H48X-ray1.45A1-238[»]
4IK1X-ray2.00A2-144[»]
4IK3X-ray2.01A149-238[»]
A2-142[»]
4IK4X-ray2.01A2-142[»]
A149-238[»]
4IK5X-ray2.50A2-142[»]
A149-238[»]
4IK8X-ray1.55A2-144[»]
A149-238[»]
4IK9X-ray1.80A2-144[»]
4J88X-ray2.08A/B2-238[»]
4J89X-ray2.10A/B2-238[»]
4J8AX-ray1.26A2-238[»]
4JFGX-ray3.00A/B/C/D/E/F/G/H1-238[»]
4JRBX-ray2.41A1-229[»]
4KA9X-ray1.58A5-238[»]
4KAGX-ray1.12A1-238[»]
4KEXX-ray1.60A1-238[»]
4KF5X-ray2.60A/B1-196[»]
4KW4X-ray1.75A2-238[»]
4KW8X-ray2.46A2-238[»]
4KW9X-ray1.80A2-238[»]
4L12X-ray1.78A2-230[»]
4L13X-ray1.66A2-230[»]
4L1IX-ray1.20A2-230[»]
4LQTX-ray1.10A2-238[»]
4LQUX-ray1.60A/B/C/D2-238[»]
4LW5X-ray2.55A/B/C/D/E2-238[»]
4N3DX-ray1.34A/B1-230[»]
4NDJX-ray1.85A2-238[»]
4NDKX-ray2.30A/B2-238[»]
4OGSX-ray2.21A/B1-238[»]
4ORNX-ray1.71A/B2-238[»]
4P1QX-ray1.50A3-231[»]
4P7HX-ray3.20A/B5-238[»]
4PA0X-ray2.25A/B5-234[»]
4PFEX-ray2.60A/B2-229[»]
4U2VX-ray2.30A/B/C/D1-230[»]
4XBIX-ray2.01A/B2-230[»]
4XGYX-ray1.49A2-238[»]
4XL5X-ray2.00A2-238[»]
4XOVX-ray1.20A2-238[»]
4XOWX-ray1.25A2-238[»]
4XVPX-ray3.40A/B/C2-238[»]
4Z4KX-ray2.80A/B1-230[»]
4Z4MX-ray2.15A/B1-230[»]
4ZF3X-ray1.90A/B51-236[»]
4ZF4X-ray1.82A/B4-237[»]
4ZF5X-ray1.70A/B4-237[»]
5AQBX-ray1.37B2-231[»]
5DPGX-ray1.85A2-238[»]
5DPHX-ray1.42A/B2-238[»]
5DPIX-ray2.54A/B/C/D/E/F2-238[»]
5DPJX-ray2.50A/B/C/D2-238[»]
5DQBX-ray1.25A2-238[»]
5DQMX-ray1.30A2-238[»]
5DRFX-ray1.14A2-238[»]
5DRGX-ray1.14A2-238[»]
5DTXX-ray1.45A1-63[»]
A65-238[»]
5DTYX-ray1.50A1-63[»]
A65-238[»]
5DTZX-ray1.50A/B/C/D2-238[»]
5DU0X-ray2.35A/B/C/D2-238[»]
5EHUX-ray1.45A/B2-238[»]
5F9GX-ray2.77A2-144[»]
5FGUX-ray1.90A1-229[»]
5HZOX-ray2.49A/B1-235[»]
5J2OX-ray1.50A2-238[»]
5KTGX-ray2.80A/B1-230[»]
ProteinModelPortaliP42212.
SMRiP42212.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP42212. 1 interactor.

Proteomic databases

PRIDEiP42212.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP42212.

Family and domain databases

Gene3Di2.40.155.10. 1 hit.
InterProiIPR009017. GFP.
IPR023413. GFP-like.
IPR011584. GFP-related.
IPR000786. Green_fluorescent_prot.
[Graphical view]
PfamiPF01353. GFP. 1 hit.
[Graphical view]
PRINTSiPR01229. GFLUORESCENT.
SUPFAMiSSF54511. SSF54511. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGFP_AEQVI
AccessioniPrimary (citable) accession number: P42212
Secondary accession number(s): Q17104, Q27903, Q93125
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Protein Spotlight
    Protein Spotlight articles and cited UniProtKB/Swiss-Prot entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.