Reviewed, UniProtKB/Swiss-Prot P42211 (ASPRX_ORYSJ)

Names and origin

Protein names

Recommended name:
    Aspartic proteinase
    EC=3.4.23.-

Gene names

Name:	RAP
Ordered Locus Names:	Os05g0137400, LOC_Os05g04630
ORF Names:	OJ1127_B08.13

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	496 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Sequence similarities

Belongs to the peptidase A1 family. Contains 1 saposin B-type domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Aspartyl protease Hydrolase Protease
PTM	Disulfide bond Glycoprotein Zymogen
Gene Ontology (GO)
Biological process	lipid metabolic process Inferred from electronic annotation. Source: InterPro proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 59	40	Activation peptide Potential		PRO_0000025910
	Chain	60 – 496	437	Aspartic proteinase		PRO_0000025911
Regions
	Domain	307 – 407	101	Saposin B-type
Sites
	Active site	95	1	By similarity
	Active site	282	1	By similarity
Amino acid modifications
	Glycosylation	387	1	N-linked (GlcNAc...) Potential
	Disulfide bond	108 ↔ 114		By similarity
	Disulfide bond	273 ↔ 277		By similarity
	Disulfide bond	415 ↔ 452		By similarity
Experimental info
	Sequence conflict	158	1	L → Q in BAA02242. Ref.1
	Sequence conflict	431	1	K → R in BAA78908. Ref.3
	Sequence conflict	492	1	F → V in BAA78908. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P42211-1 [UniParc]. Last modified October 25, 2005. Version 2. Checksum: 82F5AE431A833FE0	FASTA	496	54,090
	10 20 30 40 50 60 MAKRHLLLVT TCLWALSCAL LLHASSDGFL RVNLNKKRLD KEDLTAAKLA QQGNRLLKTG 70 80 90 100 110 120 SSDSDPVPLV DYLNTQYYGV IGLGSPPQNF TVIFDTGSSN LWVPSAKCYF SIACYLHSRY 130 140 150 160 170 180 NSKKSSSYKA DGETCKITYG SGAISGFFSK DNVLVGDLVV KNQKFIEATR ETSVTFIIGK 190 200 210 220 230 240 FDGILGLGYP EISVGKAPPI WQSMQEQELL ADDVFSFWLN RDPDASSGGE LVFGGMDPKH 250 260 270 280 290 300 YKGDHTYVPV SRKGYWQFNM GDLLIDGHST GFCAKGCAAI VDSGTSLLAG PTAIVAQVNH 310 320 330 340 350 360 AIGAEGIIST ECKEVVSEYG EMILNLLIAQ TDPQKVCSQV GLCMFDGKRS VSNGIESVVD 370 380 390 400 410 420 KENLGSDAMC SVCEMAVVWI ENQLRENKTK ELILNYANQL CERLPSPNGE STVSCHQISK 430 440 450 460 470 480 MPNLAFTIAN KTFILTPEQY IVKLEQGGQT VCISGFMAFD IPPPRGPLWI LGDVFMGAYH 490 TVFDFGKDRI GFAKSA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nucleotide sequence of a cDNA encoding aspartic proteinase in rice." Hashimoto H., Nishi R., Uchimiya H., Kato A. Submitted (AUG-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"A fine physical map of the rice chromosome 5." Cheng C.-H., Chung M.C., Liu S.-M., Chen S.-K., Kao F.Y., Lin S.-J., Hsiao S.-H., Tseng I.C., Hsing Y.-I.C., Wu H.-P., Chen C.-S., Shaw J.-F., Wu J., Matsumoto T., Sasaki T., Chen H.-C., Chow T.-Y. Mol. Genet. Genomics 274:337-345(2005) [PubMed: 16261349] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
[3]	"Chilling sensitive rice partial mRNA for aspartic protease." Tao Y., Li L. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 405-496.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D12777 mRNA. Translation: BAA02242.1. AC093490 Genomic DNA. Translation: AAS98423.1. AB028888 mRNA. Translation: BAA78908.1.
PIR	JS0732.
RefSeq	NP_001054586.1.
UniGene	Os.4168
3D structure databases
SMR	P42211. Positions 41-496.
ModBase	Search...
Genome annotation databases
GeneID	4337744.
KEGG	osa:4337744.
Organism-specific databases
Gramene	P42211.
Family and domain databases
InterPro	IPR001461. Peptidase_A1. IPR001969. Peptidase_aspartic_AS. IPR009007. Peptidase_aspartic_catalytic. IPR007856. SapB_1. IPR008138. SapB_2. IPR011001. Saposin-like. IPR008139. SaposinB. [Graphical view]
Gene3D	G3DSA:2.40.70.10. Pept_Aspartc_cat. 2 hits.
PANTHER	PTHR13683. Peptidase_A1. 1 hit.
Pfam	PF00026. Asp. 1 hit. PF05184. SapB_1. 1 hit. PF03489. SapB_2. 1 hit. [Graphical view]
PRINTS	PR00792. PEPSIN.
SMART	SM00741. SapB. 2 hits. [Graphical view]
PROSITE	PS00141. ASP_PROTEASE. 2 hits. PS50015. SAP_B. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASPRX_ORYSJ

Accession

Primary (citable) accession number: P42211
Secondary accession number(s): Q75L35, Q9XIV3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	October 25, 2005
Last modified:	November 24, 2009
This is version 70 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)