P42202 (IL4_HORSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Interleukin-4
Short name=IL-4

Alternative name(s):
B-cell stimulatory factor 1
Short name=BSF-1
Lymphocyte stimulatory factor 1

Gene names

Name:

IL4

Organism

Equus caballus (Horse) [Reference proteome]

Taxonomic identifier

9796 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Perissodactyla › Equidae › Equus › Equus

Protein attributes

Sequence length	134 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes By similarity.
Subcellular location	Secreted.
Sequence similarities	Belongs to the IL-4/IL-13 family.

Ontologies

Keywords
Biological process	B-cell activation
Cellular component	Secreted
Domain	Signal
Molecular function	Cytokine Growth factor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	B cell activation Inferred from electronic annotation. Source: UniProtKB-KW immune response Inferred from electronic annotation. Source: InterPro
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	By similarity
	Chain	24 – 134	111	Interleukin-4		PRO_0000015531
Amino acid modifications
	Glycosylation	38	1	N-linked (GlcNAc...) Potential
	Glycosylation	101	1	N-linked (GlcNAc...) Potential
	Disulfide bond	24 ↔ 133		By similarity
	Disulfide bond	48 ↔ 88		By similarity
Experimental info
	Sequence conflict	5	1	Y → S in AAA21299. Ref.1
	Sequence conflict	8	1	L → I in AAG21896. Ref.3
	Sequence conflict	19	1	S → SN in AAG21896. Ref.3
	Sequence conflict	25	1	K → I in AAB87701. Ref.2
	Sequence conflict	35	1	K → I in AAB87701. Ref.2
	Sequence conflict	38	1	N → NN in AAG21896. Ref.3
	Sequence conflict	58	1	G → AG Ref.3
	Sequence conflict	76	1	Q → QL in AAG21896. Ref.3
	Sequence conflict	96	1	Missing in AAA21299. Ref.1
	Sequence conflict	116	1	Missing in AAA21299. Ref.1
	Sequence conflict	127	1	K → R in AAA21299. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42202 [UniParc]. Last modified April 12, 2005. Version 2. Checksum: CD609F0083B90F32	FASTA	134	14,921
	10 20 30 40 50 60 MGLTYQLLPA LVCLLACTSF IQGCKYDITL QEIIKTLNLT DGKGKNSCME LTVADAFGPK 70 80 90 100 110 120 NTDGKEICRA AKVLQQYKRH DRSLIKECLS GLDRNLKGMA NGTCCTVNEA KKSTLKDFLE 130 RLKTIMKEKY SKCS

References

[1]	"Molecular cloning and sequencing of equine interleukin 4." Vandergrifft E.V., Swiderski C.E., Horohov D.W. Vet. Immunol. Immunopathol. 40:379-384(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Horse (Equus caballus) interleukin-4 gene." Schrenzel M.D., Stannard A.A., Daft B., Ferrick D.A. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"Cloning of equine interleukin 4 (IL-4)." Steinbach F., Mauel S. Submitted (SEP-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	L06010 mRNA. Translation: AAA21299.1. AF035404 mRNA. Translation: AAB87701.1. AF305617 mRNA. Translation: AAG21896.1.
RefSeq	NP_001075988.1. NM_001082519.1.
UniGene	Eca.11253.
3D structure databases
ProteinModelPortal	P42202.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100034225.
KEGG	ecb:100034225.
Organism-specific databases
CTD	3565.
Phylogenomic databases
eggNOG	NOG266404.
HOVERGEN	HBG000290.
InParanoid	P42202.
KO	K05430.
OrthoDB	EOG469QWD.
Family and domain databases
Gene3D	1.20.1250.10. 1 hit.
InterPro	IPR009079. 4_helix_cytokine-like_core. IPR012351. 4_helix_cytokine_core. IPR002354. Interleukin-4. IPR018096. Interleukin-4/-13_CS. IPR001325. Interleukin-4/13. [Graphical view]
PANTHER	PTHR11456. PTHR11456. 1 hit.
Pfam	PF00727. IL4. 1 hit. [Graphical view]
PIRSF	PIRSF001941. Interleukin_4. 1 hit.
PRINTS	PR00431. INTERLEUKIN4.
SMART	SM00190. IL4_13. 1 hit. [Graphical view]
SUPFAM	SSF47266. 4_helix_cytokine. 1 hit.
PROSITE	PS00838. INTERLEUKIN_4_13. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IL4_HORSE

Accession

Primary (citable) accession number: P42202
Secondary accession number(s): O46393, Q9GL85

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	April 12, 2005
Last modified:	April 3, 2013
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program