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Protein

P-selectin

Gene

SELP

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Ca2+-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1 (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Lectin

Names & Taxonomyi

Protein namesi
Recommended name:
P-selectin
Alternative name(s):
CD62 antigen-like family member P
Granule membrane protein 140
Short name:
GMP-140
Leukocyte-endothelial cell adhesion molecule 3
Short name:
LECAM3
Platelet activation dependent granule-external membrane protein
Short name:
PADGEM
CD_antigen: CD62P
Gene namesi
Name:SELP
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini42 – 587546ExtracellularSequence analysisAdd
BLAST
Transmembranei588 – 61124HelicalSequence analysisAdd
BLAST
Topological domaini612 – 64635CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4141By similarityAdd
BLAST
Chaini42 – 646605P-selectinPRO_0000017497Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi48 – 481N-linked (GlcNAc...)Sequence analysis
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence analysis
Disulfide bondi60 ↔ 158By similarity
Glycosylationi80 – 801N-linked (GlcNAc...)Sequence analysis
Disulfide bondi131 ↔ 150By similarity
Disulfide bondi163 ↔ 174By similarity
Disulfide bondi168 ↔ 183By similarity
Glycosylationi180 – 1801N-linked (GlcNAc...)Sequence analysis
Disulfide bondi185 ↔ 194By similarity
Disulfide bondi200 ↔ 244By similarity
Glycosylationi212 – 2121N-linked (GlcNAc...)Sequence analysis
Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence analysis
Disulfide bondi230 ↔ 257By similarity
Disulfide bondi262 ↔ 306By similarity
Disulfide bondi292 ↔ 319By similarity
Disulfide bondi324 ↔ 368By similarity
Glycosylationi336 – 3361N-linked (GlcNAc...)Sequence analysis
Disulfide bondi354 ↔ 381By similarity
Disulfide bondi386 ↔ 430By similarity
Disulfide bondi416 ↔ 443By similarity
Disulfide bondi458 ↔ 502By similarity
Glycosylationi481 – 4811N-linked (GlcNAc...)Sequence analysis
Disulfide bondi488 ↔ 515By similarity
Disulfide bondi520 ↔ 564By similarity
Glycosylationi532 – 5321N-linked (GlcNAc...)Sequence analysis
Glycosylationi539 – 5391N-linked (GlcNAc...)Sequence analysis
Disulfide bondi550 ↔ 577By similarity
Glycosylationi557 – 5571N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP42201.

Expressioni

Tissue specificityi

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Interactioni

Subunit structurei

Interacts with SNX17. Interacts with PSGL1/SEPL and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of PSGL1 and PODXL2, and specific tyrosine sulfation on PSGL1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliP42201.
SMRiP42201. Positions 42-199.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini58 – 158101C-type lectinPROSITE-ProRule annotationAdd
BLAST
Domaini159 – 19537EGF-likePROSITE-ProRule annotationAdd
BLAST
Domaini198 – 25962Sushi 1PROSITE-ProRule annotationAdd
BLAST
Domaini260 – 32162Sushi 2PROSITE-ProRule annotationAdd
BLAST
Domaini322 – 38362Sushi 3PROSITE-ProRule annotationAdd
BLAST
Domaini384 – 44562Sushi 4PROSITE-ProRule annotationAdd
BLAST
Domaini456 – 51762Sushi 5PROSITE-ProRule annotationAdd
BLAST
Domaini518 – 57962Sushi 6PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni637 – 64610Interaction with SNX17By similarity

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi634 – 6374Endocytosis signalCurated

Sequence similaritiesi

Belongs to the selectin/LECAM family.Curated
Contains 1 C-type lectin domain.PROSITE-ProRule annotation
Contains 1 EGF-like domain.PROSITE-ProRule annotation
Contains 6 Sushi (CCP/SCR) domains.PROSITE-ProRule annotation

Keywords - Domaini

EGF-like domain, Repeat, Signal, Sushi, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000236254.
HOVERGENiHBG052375.
InParanoidiP42201.
KOiK06496.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 6 hits.
[Graphical view]
PRINTSiPR00343. SELECTIN.
SMARTiSM00032. CCP. 6 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 2 hits.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57535. SSF57535. 6 hits.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 6 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42201-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASCPKAIWN WRFQRAVFRT VQLLCFSVLI FEVINQKEVS AWTYHYSNKT
60 70 80 90 100
YSWNYSRAFC QKYYTDLVAI QNKNEIAYLN ETIPYYNSYY WIGIRKINNK
110 120 130 140 150
WTWVGTKKTL TEEAENWADN EPNNKRNNQD CVEIYIKSLS APGKWNDEPC
160 170 180 190 200
WKRKRALCYR ASCQDMSCSK QGECIETIGN YTCSCYPGFY GPECEYVREC
210 220 230 240 250
GEFDLPQHVH MNCSHPLGNF SFNSHCSFHC AEGYALNGPS ELECLASGIW
260 270 280 290 300
TNSPPQCVAV QCPALKSPEQ GSMSCVQSAE AFQHQSSCSF SCEEGFALVG
310 320 330 340 350
PEVVHCTALG VWTAPTPVCK ALQCQDLPTS TKARVNCSHP FGDFRYQSTC
360 370 380 390 400
SFTCDEGSFL VGASVLQCLD TGNWDAPFPE CQAVTCAALP NPQNGEKTCV
410 420 430 440 450
QPLGGSSYES TCWFTCHEGF SLSGPERLDC TPSGHWTGSP PTCEEVDTVS
460 470 480 490 500
APAPGVQCPT LIAPKQGTMS CQHHVRNFGL NTTCHFGCKA GFTLLGDSAL
510 520 530 540 550
QCRPSRQWTA AAPTCRAVKC AKLPVTEPIV MNCSNPWGNF SYGSTCSFHC
560 570 580 590 600
PEGQLLNGSE RTVCQENGQW STTMPTCQAG PLTIQETLTY VGGAAAGTTG
610 620 630 640
LVTGSILLAL LRRRCRQKDD GKSPLNPQSH LGTYGVFTNA AFDPSP
Length:646
Mass (Da):71,229
Last modified:November 1, 1995 - v1
Checksum:i573912A4627A6ACA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12041 mRNA. Translation: AAA30743.1.
PIRiJN0473.
RefSeqiNP_776608.1. NM_174183.2.
UniGeneiBt.4342.

Genome annotation databases

GeneIDi281486.
KEGGibta:281486.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L12041 mRNA. Translation: AAA30743.1.
PIRiJN0473.
RefSeqiNP_776608.1. NM_174183.2.
UniGeneiBt.4342.

3D structure databases

ProteinModelPortaliP42201.
SMRiP42201. Positions 42-199.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP42201.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281486.
KEGGibta:281486.

Organism-specific databases

CTDi6403.

Phylogenomic databases

HOGENOMiHOG000236254.
HOVERGENiHBG052375.
InParanoidiP42201.
KOiK06496.

Family and domain databases

Gene3Di3.10.100.10. 1 hit.
InterProiIPR001304. C-type_lectin.
IPR016186. C-type_lectin-like.
IPR018378. C-type_lectin_CS.
IPR016187. C-type_lectin_fold.
IPR013032. EGF-like_CS.
IPR000742. EGF-like_dom.
IPR002396. Selectin_superfamily.
IPR000436. Sushi_SCR_CCP_dom.
[Graphical view]
PfamiPF00008. EGF. 1 hit.
PF00059. Lectin_C. 1 hit.
PF00084. Sushi. 6 hits.
[Graphical view]
PRINTSiPR00343. SELECTIN.
SMARTiSM00032. CCP. 6 hits.
SM00034. CLECT. 1 hit.
SM00181. EGF. 2 hits.
[Graphical view]
SUPFAMiSSF56436. SSF56436. 1 hit.
SSF57535. SSF57535. 6 hits.
PROSITEiPS00615. C_TYPE_LECTIN_1. 1 hit.
PS50041. C_TYPE_LECTIN_2. 1 hit.
PS00022. EGF_1. 1 hit.
PS01186. EGF_2. 1 hit.
PS50026. EGF_3. 1 hit.
PS50923. SUSHI. 6 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Isolation and characterization of a bovine cDNA encoding a functional homolog of human P-selectin."
    Strubel N.A., Nguyen M., Kansas G.S., Tedder T.F., Bischoff J.
    Biochem. Biophys. Res. Commun. 192:338-344(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Capillary endothelium.

Entry informationi

Entry nameiLYAM3_BOVIN
AccessioniPrimary (citable) accession number: P42201
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.