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P42196 (BACS2_NATPH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sensory rhodopsin-2
Alternative name(s):
Sensory rhodopsin II
Short name=SR-II
Gene names
Name:sop2
Synonyms:sopII
OrganismNatronomonas pharaonis (Natronobacterium pharaonis)
Taxonomic identifier2257 [NCBI]
Taxonomic lineageArchaeaEuryarchaeotaHalobacteriaHalobacterialesHalobacteriaceaeNatronomonas

Protein attributes

Sequence length239 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Photophobic photoreceptor responsible for the negative phototaxis. Activates the sensory rhodopsin II transducer (HTR-II) in response to blue light. Ref.2 Ref.3 Ref.5

Subunit structure

Homodimer. Interacts with HTR-II. Ref.5 Ref.6

Subcellular location

Cell membrane; Multi-pass membrane protein Ref.2 Ref.7.

Sequence similarities

Belongs to the archaeal/bacterial/fungal opsin family.

Ontologies

Keywords
   Biological processSensory transduction
   Cellular componentCell membrane
Membrane
   DomainTransmembrane
Transmembrane helix
   LigandChromophore
   Molecular functionPhotoreceptor protein
Receptor
Retinal protein
   Technical term3D-structure
Gene Ontology (GO)
   Biological_processphototransduction

Inferred from electronic annotation. Source: UniProtKB-KW

protein-chromophore linkage

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionion channel activity

Inferred from electronic annotation. Source: InterPro

photoreceptor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

htr2P422591EBI-1034509,EBI-1034515

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 239239Sensory rhodopsin-2
PRO_0000196282

Regions

Topological domain1 – 33Extracellular
Transmembrane4 – 2522Helical; Name=Helix A
Topological domain26 – 338Cytoplasmic
Transmembrane34 – 5522Helical; Name=Helix B
Topological domain56 – 6914Extracellular
Transmembrane70 – 9122Helical; Name=Helix C
Topological domain92 – 943Cytoplasmic
Transmembrane95 – 11723Helical; Name=Helix D
Topological domain118 – 1214Extracellular
Transmembrane122 – 14928Helical; Name=Helix E
Topological domain150 – 1534Cytoplasmic
Transmembrane154 – 18128Helical; Name=Helix F
Topological domain182 – 1898Extracellular
Transmembrane190 – 22233Helical; Name=Helix G
Topological domain223 – 23917Cytoplasmic

Amino acid modifications

Modified residue2051N6-(retinylidene)lysine

Secondary structure

......................... 239
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P42196 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: C0E93EAF341B6B19

FASTA23925,355
        10         20         30         40         50         60 
MVGLTTLFWL GAIGMLVGTL AFAWAGRDAG SGERRYYVTL VGISGIAAVA YVVMALGVGW 

        70         80         90        100        110        120 
VPVAERTVFA PRYIDWILTT PLIVYFLGLL AGLDSREFGI VITLNTVVML AGFAGAMVPG 

       130        140        150        160        170        180 
IERYALFGMG AVAFLGLVYY LVGPMTESAS QRSSGIKSLY VRLRNLTVIL WAIYPFIWLL 

       190        200        210        220        230 
GPPGVALLTP TVDVALIVYL DLVTKVGFGF IALDAAATLR AEHGESLAGV DTDAPAVAD 

« Hide

References

[1]"The primary structure of sensory rhodopsin II: a member of an additional retinal protein subgroup is coexpressed with its transducer, the halobacterial transducer of rhodopsin II."
Seidel R., Scharf B., Gautel M., Kleine K., Oesterhelt D., Engelhard M.
Proc. Natl. Acad. Sci. U.S.A. 92:3036-3040(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: SP-1 / 28.
[2]"Biochemical and photochemical properties of the photophobic receptors from Halobacterium halobium and Natronobacterium pharaonis."
Scharf B., Pevec B., Hess B., Engelhard M.
Eur. J. Biochem. 206:359-366(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
Strain: SP-1 / 28.
[3]"Heterologous coexpression of the blue light receptor psRII and its transducer pHtrII from Natronobacterium pharaonis in the Halobacterium salinarium strain Pho81/w restores negative phototaxis."
Luttenberg B., Wolff E.K., Engelhard M.
FEBS Lett. 426:117-120(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[4]"Crystal structure of sensory rhodopsin II at 2.4 Angstroms: insights into color tuning and transducer interaction."
Luecke H., Schobert B., Lanyi J.K., Spudich E.N., Spudich J.L.
Science 293:1499-1503(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS) OF 1-217, RETINAL-BINDING SITE.
[5]"Molecular basis of transmembrane signalling by sensory rhodopsin II-transducer complex."
Gordeliy V.I., Labahn J., Moukhametzianov R., Efremov R., Granzin J., Schlesinger R., Bueldt G., Savopol T., Scheidig A.J., Klare J.P., Engelhard M.
Nature 419:484-487(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 1-225, FUNCTION, INTERACTION WITH HTR-II, RETINAL-BINDING SITE.
[6]"X-ray structure of sensory rhodopsin II at 2.1-A resolution."
Royant A., Nollert P., Edman K., Neutze R., Landau E.M., Pebay-Peyroula E., Navarro J.
Proc. Natl. Acad. Sci. U.S.A. 98:10131-10136(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 2-219, SUBUNIT, RETINAL-BINDING SITE.
[7]"Early structural rearrangements in the photocycle of an integral membrane sensory receptor."
Edman K., Royant A., Nollert P., Maxwell C.A., Pebay-Peyroula E., Navarro J., Neutze R., Landau E.M.
Structure 10:473-482(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.27 ANGSTROMS) OF 2-219, SUBCELLULAR LOCATION, RETINAL-BINDING SITE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z35086 Genomic DNA. Translation: CAA84469.1.
PIRS55300.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1GU8X-ray2.27A1-239[»]
1GUEX-ray2.27A1-239[»]
1H2SX-ray1.93A1-225[»]
1H68X-ray2.10A1-239[»]
1JGJX-ray2.40A1-217[»]
2F93X-ray2.00A2-239[»]
2F95X-ray2.20A2-239[»]
3QAPX-ray1.90A1-239[»]
3QDCX-ray2.50A1-239[»]
4GYCX-ray2.05A1-239[»]
ProteinModelPortalP42196.
SMRP42196. Positions 1-225.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-35282N.
IntActP42196. 1 interaction.
MINTMINT-248654.

Protein family/group databases

TCDB3.E.1.3.3. the ion-translocating microbial rhodopsin (mr) family.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D1.20.1070.10. 1 hit.
InterProIPR017452. GPCR_Rhodpsn_7TM.
IPR001425. Rhodopsin_arc/bac/fun.
IPR018229. Rhodopsin_retinal_BS.
[Graphical view]
PfamPF01036. Bac_rhodopsin. 1 hit.
[Graphical view]
PRINTSPR00251. BACTRLOPSIN.
SMARTSM01021. Bac_rhodopsin. 1 hit.
[Graphical view]
PROSITEPS00950. BACTERIAL_OPSIN_1. 1 hit.
PS00327. BACTERIAL_OPSIN_RET. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP42196.

Entry information

Entry nameBACS2_NATPH
AccessionPrimary (citable) accession number: P42196
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries