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Protein

HTH-type transcriptional regulator LrpA

Gene

lrpA

Organism
Pyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Negatively regulates its own transcription. Binds to a 46-base pair sequence that overlaps the transcriptional start site of its own promoter.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi21 – 4020H-T-H motifPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
HTH-type transcriptional regulator LrpA
Gene namesi
Name:lrpA
Ordered Locus Names:PF1601
OrganismiPyrococcus furiosus (strain ATCC 43587 / DSM 3638 / JCM 8422 / Vc1)
Taxonomic identifieri186497 [NCBI]
Taxonomic lineageiArchaeaEuryarchaeotaThermococciThermococcalesThermococcaceaePyrococcus
Proteomesi
  • UP000001013 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 141141HTH-type transcriptional regulator LrpAPRO_0000111771Add
BLAST

Proteomic databases

PRIDEiP42180.

Interactioni

Subunit structurei

Binds DNA as a homotetramer.

Protein-protein interaction databases

STRINGi186497.PF1601.

Structurei

Secondary structure

1
141
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi5 – 1511Combined sources
Helixi21 – 288Combined sources
Helixi32 – 4514Combined sources
Helixi58 – 603Combined sources
Beta strandi64 – 729Combined sources
Helixi74 – 763Combined sources
Helixi77 – 8610Combined sources
Beta strandi90 – 934Combined sources
Beta strandi97 – 11014Combined sources
Helixi111 – 12010Combined sources
Turni121 – 1244Combined sources
Beta strandi128 – 1358Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I1GX-ray2.90A/B1-141[»]
ProteinModelPortaliP42180.
SMRiP42180. Positions 2-141.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42180.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 6362HTH asnC-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HTH asnC-type DNA-binding domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiarCOG01580. Archaea.
COG1522. LUCA.
HOGENOMiHOG000115326.
KOiK03718.
OMAiMAVIWAK.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC/Lrp_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIDERDKIIL EILEKDARTP FTEIAKKLGI SETAVRKRVK ALEEKGIIEG
60 70 80 90 100
YTIKINPKKL GYSLVTITGV DTKPEKLFEV AEKLKEYDFV KELYLSSGDH
110 120 130 140
MIMAVIWAKD GEDLAEIISN KIGKIEGVTK VCPAIILEKL K
Length:141
Mass (Da):15,892
Last modified:September 26, 2001 - v2
Checksum:i41A74AD10119C925
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97860 Genomic DNA. Translation: AAD20389.1.
AE009950 Genomic DNA. Translation: AAL81725.1.
PIRiT46972.
RefSeqiWP_011012747.1. NC_003413.1.

Genome annotation databases

EnsemblBacteriaiAAL81725; AAL81725; PF1601.
GeneIDi1469477.
KEGGipfu:PF1601.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M97860 Genomic DNA. Translation: AAD20389.1.
AE009950 Genomic DNA. Translation: AAL81725.1.
PIRiT46972.
RefSeqiWP_011012747.1. NC_003413.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1I1GX-ray2.90A/B1-141[»]
ProteinModelPortaliP42180.
SMRiP42180. Positions 2-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi186497.PF1601.

Proteomic databases

PRIDEiP42180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAL81725; AAL81725; PF1601.
GeneIDi1469477.
KEGGipfu:PF1601.

Phylogenomic databases

eggNOGiarCOG01580. Archaea.
COG1522. LUCA.
HOGENOMiHOG000115326.
KOiK03718.
OMAiMAVIWAK.

Miscellaneous databases

EvolutionaryTraceiP42180.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
3.30.70.920. 1 hit.
InterProiIPR000485. AsnC-type_HTH_dom.
IPR011008. Dimeric_a/b-barrel.
IPR019888. Tscrpt_reg_AsnC-typ.
IPR019887. Tscrpt_reg_AsnC/Lrp_C.
IPR019885. Tscrpt_reg_HTH_AsnC-type_CS.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamiPF01037. AsnC_trans_reg. 1 hit.
[Graphical view]
PRINTSiPR00033. HTHASNC.
SMARTiSM00344. HTH_ASNC. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 1 hit.
SSF54909. SSF54909. 1 hit.
PROSITEiPS00519. HTH_ASNC_1. 1 hit.
PS50956. HTH_ASNC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The glutamate dehydrogenase-encoding gene of the hyperthermophilic archaeon Pyrococcus furiosus: sequence, transcription and analysis of the deduced amino acid sequence."
    Eggen R.I.L., Geerling A.C.M., Waldkoetter K., Antranikian G., de Vos W.M.
    Gene 132:143-148(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  2. "An Lrp-like transcriptional regulator from the archaeon Pyrococcus furiosus is negatively autoregulated."
    Brinkman A.B., Dahlke I., Tuininga J.E., Lammers T., Dumay V., de Heus E., Lebbink J.H.G., Thomm M., de Vos W.M., van Der Oost J.
    J. Biol. Chem. 275:38160-38169(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], SEQUENCE REVISION, CHARACTERIZATION.
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  3. "Divergence of the hyperthermophilic archaea Pyrococcus furiosus and P. horikoshii inferred from complete genomic sequences."
    Maeder D.L., Weiss R.B., Dunn D.M., Cherry J.L., Gonzalez J.M., DiRuggiero J., Robb F.T.
    Genetics 152:1299-1305(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43587 / DSM 3638 / JCM 8422 / Vc1.
  4. "The eubacterial transcriptional activator Lrp is present in the archaeon Pyrococcus furiosus."
    Kyrpides N.C., Ouzounis C.A.
    Trends Biochem. Sci. 20:140-141(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, SIMILARITY.

Entry informationi

Entry nameiREG7_PYRFU
AccessioniPrimary (citable) accession number: P42180
Secondary accession number(s): Q9Y8K7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: September 26, 2001
Last modified: May 11, 2016
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.