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P42177

- NARI_BACSU

UniProt

P42177 - NARI_BACSU

Protein

Nitrate reductase gamma chain

Gene

narI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 101 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the extracellular interface. Electrons are transferred from the extracellular to the cytoplasmic heme By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Iron (heme B 1 axial ligand)By similarity
    Metal bindingi64 – 641Iron (heme B 2 axial ligand)By similarity
    Metal bindingi185 – 1851Iron (heme B 2 axial ligand)By similarity
    Metal bindingi203 – 2031Iron (heme B 1 axial ligand)By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU37250-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrate reductase gamma chain (EC:1.7.99.4)
    Gene namesi
    Name:narI
    Ordered Locus Names:BSU37250
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU37250. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. nitrate reductase complex Source: InterPro
    3. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 223223Nitrate reductase gamma chainPRO_0000096722Add
    BLAST

    Proteomic databases

    PaxDbiP42177.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU37250.

    Structurei

    3D structure databases

    ProteinModelPortaliP42177.
    SMRiP42177. Positions 1-223.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini28 – 4518CytoplasmicBy similarityAdd
    BLAST
    Topological domaini69 – 8113ExtracellularBy similarityAdd
    BLAST
    Topological domaini112 – 12312CytoplasmicBy similarityAdd
    BLAST
    Topological domaini148 – 18033ExtracellularBy similarityAdd
    BLAST
    Topological domaini197 – 22327CytoplasmicBy similarityAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei2 – 2726Helical; Name=1By similarityAdd
    BLAST
    Transmembranei46 – 6823Helical; Name=2By similarityAdd
    BLAST
    Transmembranei82 – 11130Helical; Name=3By similarityAdd
    BLAST
    Transmembranei124 – 14724Helical; Name=4By similarityAdd
    BLAST
    Transmembranei181 – 19616Helical; Name=5By similarityAdd
    BLAST

    Family & Domainsi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2181.
    HOGENOMiHOG000237377.
    KOiK00374.
    OMAiWTEAVGV.
    OrthoDBiEOG6K13W6.
    PhylomeDBiP42177.

    Family and domain databases

    InterProiIPR023234. NarG-like_domain.
    IPR003816. Nitrate_red_gam.
    [Graphical view]
    PfamiPF02665. Nitrate_red_gam. 1 hit.
    [Graphical view]
    SUPFAMiSSF103501. SSF103501. 1 hit.
    TIGRFAMsiTIGR00351. narI. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P42177-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGQILWGIM PYIVLTIFIG GHIYRYQHDQ FGWTAKSSEL LEKKKLAAGS    50
    TLFHWGLLCV VGGHVMGILI PEGVYASLGI SEHMYHKMAI GAGLPAGIAA 100
    CTGLVILTYR RLFDKRIRKT SSPSDILTLL LLLFMMLSGV AATFLNIDSK 150
    GFDYRTTVGP WFREIVLFRP DASLMESVPL WFKFHIVIGY VVFILWPFTR 200
    LVHVFSLPLK YLTRSYVVYR KRS 223
    Length:223
    Mass (Da):25,296
    Last modified:November 1, 1995 - v1
    Checksum:i9C4379C5DF780000
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49884 Genomic DNA. Translation: CAA90048.1.
    AL009126 Genomic DNA. Translation: CAB15753.1.
    X85014 Genomic DNA. Translation: CAA59374.1.
    X91819 Genomic DNA. Translation: CAA62929.1.
    PIRiS60088.
    RefSeqiNP_391606.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15753; CAB15753; BSU37250.
    GeneIDi938454.
    KEGGibsu:BSU37250.
    PATRICi18979488. VBIBacSub10457_3906.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49884 Genomic DNA. Translation: CAA90048.1 .
    AL009126 Genomic DNA. Translation: CAB15753.1 .
    X85014 Genomic DNA. Translation: CAA59374.1 .
    X91819 Genomic DNA. Translation: CAA62929.1 .
    PIRi S60088.
    RefSeqi NP_391606.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P42177.
    SMRi P42177. Positions 1-223.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU37250.

    Proteomic databases

    PaxDbi P42177.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15753 ; CAB15753 ; BSU37250 .
    GeneIDi 938454.
    KEGGi bsu:BSU37250.
    PATRICi 18979488. VBIBacSub10457_3906.

    Organism-specific databases

    GenoListi BSU37250. [Micado ]

    Phylogenomic databases

    eggNOGi COG2181.
    HOGENOMi HOG000237377.
    KOi K00374.
    OMAi WTEAVGV.
    OrthoDBi EOG6K13W6.
    PhylomeDBi P42177.

    Enzyme and pathway databases

    BioCyci BSUB:BSU37250-MONOMER.

    Family and domain databases

    InterProi IPR023234. NarG-like_domain.
    IPR003816. Nitrate_red_gam.
    [Graphical view ]
    Pfami PF02665. Nitrate_red_gam. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103501. SSF103501. 1 hit.
    TIGRFAMsi TIGR00351. narI. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
      Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
      EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
      Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
      Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    4. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
      Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
      FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-160.
      Strain: 168 / JH642.

    Entry informationi

    Entry nameiNARI_BACSU
    AccessioniPrimary (citable) accession number: P42177
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 101 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

    External Data

    Dasty 3