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P42177 (NARI_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrate reductase gamma chain

EC=1.7.99.4
Gene names
Name:narI
Ordered Locus Names:BSU37250
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the extracellular interface. Electrons are transferred from the extracellular to the cytoplasmic heme By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 223223Nitrate reductase gamma chain
PRO_0000096722

Regions

Transmembrane2 – 2726Helical; Name=1; By similarity
Topological domain28 – 4518Cytoplasmic By similarity
Transmembrane46 – 6823Helical; Name=2; By similarity
Topological domain69 – 8113Extracellular By similarity
Transmembrane82 – 11130Helical; Name=3; By similarity
Topological domain112 – 12312Cytoplasmic By similarity
Transmembrane124 – 14724Helical; Name=4; By similarity
Topological domain148 – 18033Extracellular By similarity
Transmembrane181 – 19616Helical; Name=5; By similarity
Topological domain197 – 22327Cytoplasmic By similarity

Sites

Metal binding541Iron (heme B 1 axial ligand) By similarity
Metal binding641Iron (heme B 2 axial ligand) By similarity
Metal binding1851Iron (heme B 2 axial ligand) By similarity
Metal binding2031Iron (heme B 1 axial ligand) By similarity

Sequences

Sequence LengthMass (Da)Tools
P42177 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 9C4379C5DF780000

FASTA22325,296
        10         20         30         40         50         60 
MSGQILWGIM PYIVLTIFIG GHIYRYQHDQ FGWTAKSSEL LEKKKLAAGS TLFHWGLLCV 

        70         80         90        100        110        120 
VGGHVMGILI PEGVYASLGI SEHMYHKMAI GAGLPAGIAA CTGLVILTYR RLFDKRIRKT 

       130        140        150        160        170        180 
SSPSDILTLL LLLFMMLSGV AATFLNIDSK GFDYRTTVGP WFREIVLFRP DASLMESVPL 

       190        200        210        220 
WFKFHIVIGY VVFILWPFTR LVHVFSLPLK YLTRSYVVYR KRS 

« Hide

References

« Hide 'large scale' references
[1]"Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[4]"The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-160.
Strain: 168 / JH642.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49884 Genomic DNA. Translation: CAA90048.1.
AL009126 Genomic DNA. Translation: CAB15753.1.
X85014 Genomic DNA. Translation: CAA59374.1.
X91819 Genomic DNA. Translation: CAA62929.1.
PIRS60088.
RefSeqNP_391606.1. NC_000964.3.

3D structure databases

ProteinModelPortalP42177.
SMRP42177. Positions 1-223.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU37250.

Proteomic databases

PaxDbP42177.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15753; CAB15753; BSU37250.
GeneID938454.
KEGGbsu:BSU37250.
PATRIC18979488. VBIBacSub10457_3906.

Organism-specific databases

GenoListBSU37250. [Micado]

Phylogenomic databases

eggNOGCOG2181.
HOGENOMHOG000237377.
KOK00374.
OMAWTEAVGV.
OrthoDBEOG6K13W6.
PhylomeDBP42177.

Enzyme and pathway databases

BioCycBSUB:BSU37250-MONOMER.

Family and domain databases

InterProIPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view]
PfamPF02665. Nitrate_red_gam. 1 hit.
[Graphical view]
SUPFAMSSF103501. SSF103501. 1 hit.
TIGRFAMsTIGR00351. narI. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNARI_BACSU
AccessionPrimary (citable) accession number: P42177
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList