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P42177

- NARI_BACSU

UniProt

P42177 - NARI_BACSU

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Protein

Nitrate reductase gamma chain

Gene

narI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 2 heme groups per subunit. Heme 1 is located at the cytoplasmic interface, heme 2 is located at the extracellular interface. Electrons are transferred from the extracellular to the cytoplasmic heme (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Iron (heme B 1 axial ligand)By similarity
Metal bindingi64 – 641Iron (heme B 2 axial ligand)By similarity
Metal bindingi185 – 1851Iron (heme B 2 axial ligand)By similarity
Metal bindingi203 – 2031Iron (heme B 1 axial ligand)By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU37250-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase gamma chain (EC:1.7.99.4)
Gene namesi
Name:narI
Ordered Locus Names:BSU37250
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU37250. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. nitrate reductase complex Source: InterPro
  3. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 223223Nitrate reductase gamma chainPRO_0000096722Add
BLAST

Proteomic databases

PaxDbiP42177.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU37250.

Structurei

3D structure databases

ProteinModelPortaliP42177.
SMRiP42177. Positions 1-223.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini28 – 4518CytoplasmicBy similarityAdd
BLAST
Topological domaini69 – 8113ExtracellularBy similarityAdd
BLAST
Topological domaini112 – 12312CytoplasmicBy similarityAdd
BLAST
Topological domaini148 – 18033ExtracellularBy similarityAdd
BLAST
Topological domaini197 – 22327CytoplasmicBy similarityAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei2 – 2726Helical; Name=1By similarityAdd
BLAST
Transmembranei46 – 6823Helical; Name=2By similarityAdd
BLAST
Transmembranei82 – 11130Helical; Name=3By similarityAdd
BLAST
Transmembranei124 – 14724Helical; Name=4By similarityAdd
BLAST
Transmembranei181 – 19616Helical; Name=5By similarityAdd
BLAST

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2181.
HOGENOMiHOG000237377.
InParanoidiP42177.
KOiK00374.
OMAiWTEAVGV.
OrthoDBiEOG6K13W6.
PhylomeDBiP42177.

Family and domain databases

InterProiIPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view]
PfamiPF02665. Nitrate_red_gam. 1 hit.
[Graphical view]
SUPFAMiSSF103501. SSF103501. 1 hit.
TIGRFAMsiTIGR00351. narI. 1 hit.

Sequencei

Sequence statusi: Complete.

P42177 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGQILWGIM PYIVLTIFIG GHIYRYQHDQ FGWTAKSSEL LEKKKLAAGS
60 70 80 90 100
TLFHWGLLCV VGGHVMGILI PEGVYASLGI SEHMYHKMAI GAGLPAGIAA
110 120 130 140 150
CTGLVILTYR RLFDKRIRKT SSPSDILTLL LLLFMMLSGV AATFLNIDSK
160 170 180 190 200
GFDYRTTVGP WFREIVLFRP DASLMESVPL WFKFHIVIGY VVFILWPFTR
210 220
LVHVFSLPLK YLTRSYVVYR KRS
Length:223
Mass (Da):25,296
Last modified:November 1, 1995 - v1
Checksum:i9C4379C5DF780000
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49884 Genomic DNA. Translation: CAA90048.1.
AL009126 Genomic DNA. Translation: CAB15753.1.
X85014 Genomic DNA. Translation: CAA59374.1.
X91819 Genomic DNA. Translation: CAA62929.1.
PIRiS60088.
RefSeqiNP_391606.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15753; CAB15753; BSU37250.
GeneIDi938454.
KEGGibsu:BSU37250.
PATRICi18979488. VBIBacSub10457_3906.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49884 Genomic DNA. Translation: CAA90048.1 .
AL009126 Genomic DNA. Translation: CAB15753.1 .
X85014 Genomic DNA. Translation: CAA59374.1 .
X91819 Genomic DNA. Translation: CAA62929.1 .
PIRi S60088.
RefSeqi NP_391606.1. NC_000964.3.

3D structure databases

ProteinModelPortali P42177.
SMRi P42177. Positions 1-223.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU37250.

Proteomic databases

PaxDbi P42177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15753 ; CAB15753 ; BSU37250 .
GeneIDi 938454.
KEGGi bsu:BSU37250.
PATRICi 18979488. VBIBacSub10457_3906.

Organism-specific databases

GenoListi BSU37250. [Micado ]

Phylogenomic databases

eggNOGi COG2181.
HOGENOMi HOG000237377.
InParanoidi P42177.
KOi K00374.
OMAi WTEAVGV.
OrthoDBi EOG6K13W6.
PhylomeDBi P42177.

Enzyme and pathway databases

BioCyci BSUB:BSU37250-MONOMER.

Family and domain databases

InterProi IPR023234. NarG-like_domain.
IPR003816. Nitrate_red_gam.
[Graphical view ]
Pfami PF02665. Nitrate_red_gam. 1 hit.
[Graphical view ]
SUPFAMi SSF103501. SSF103501. 1 hit.
TIGRFAMsi TIGR00351. narI. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
    Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
    EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  4. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
    Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
    FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-160.
    Strain: 168 / JH642.

Entry informationi

Entry nameiNARI_BACSU
AccessioniPrimary (citable) accession number: P42177
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList

External Data

Dasty 3