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Protein

Nitrate reductase gamma chain

Gene

narI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transfers electrons from quinones to the beta subunit.

Catalytic activityi

Nitrate + a quinol = nitrite + a quinone + H2O.

Cofactori

hemeBy similarityNote: Binds 2 heme groups per subunit. Heme 1, called the proximal or heme Bp in, is located at the cytoplasmic interface, heme 2, called the distal or heme Bd, is located at the periplasmic interface. Electrons are transferred from the periplasmic to the cytoplasmic heme.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Iron (heme B 1 axial ligand)By similarity1
Metal bindingi64Iron (heme B 2 axial ligand)By similarity1
Metal bindingi185Iron (heme B 2 axial ligand)By similarity1
Metal bindingi203Iron (heme B 1 axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU37250-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase gamma chain (EC:1.7.5.1)
Gene namesi
Name:narI
Ordered Locus Names:BSU37250
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei2 – 27Helical; Name=1By similarityAdd BLAST26
Topological domaini28 – 45CytoplasmicBy similarityAdd BLAST18
Transmembranei46 – 68Helical; Name=2By similarityAdd BLAST23
Topological domaini69 – 81ExtracellularBy similarityAdd BLAST13
Transmembranei82 – 111Helical; Name=3By similarityAdd BLAST30
Topological domaini112 – 123CytoplasmicBy similarityAdd BLAST12
Transmembranei124 – 147Helical; Name=4By similarityAdd BLAST24
Topological domaini148 – 180ExtracellularBy similarityAdd BLAST33
Transmembranei181 – 196Helical; Name=5By similarityAdd BLAST16
Topological domaini197 – 223CytoplasmicBy similarityAdd BLAST27

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000967221 – 223Nitrate reductase gamma chainAdd BLAST223

Proteomic databases

PaxDbiP42177
PRIDEiP42177

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020136

Structurei

3D structure databases

ProteinModelPortaliP42177
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2181 LUCA
HOGENOMiHOG000237377
InParanoidiP42177
KOiK00374
OMAiMVSGGFF
PhylomeDBiP42177

Family and domain databases

InterProiView protein in InterPro
IPR023234 NarG-like_domain
IPR036197 NarG-like_sf
IPR003816 Nitrate_red_gam
PfamiView protein in Pfam
PF02665 Nitrate_red_gam, 1 hit
SUPFAMiSSF103501 SSF103501, 1 hit
TIGRFAMsiTIGR00351 narI, 1 hit

Sequencei

Sequence statusi: Complete.

P42177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGQILWGIM PYIVLTIFIG GHIYRYQHDQ FGWTAKSSEL LEKKKLAAGS
60 70 80 90 100
TLFHWGLLCV VGGHVMGILI PEGVYASLGI SEHMYHKMAI GAGLPAGIAA
110 120 130 140 150
CTGLVILTYR RLFDKRIRKT SSPSDILTLL LLLFMMLSGV AATFLNIDSK
160 170 180 190 200
GFDYRTTVGP WFREIVLFRP DASLMESVPL WFKFHIVIGY VVFILWPFTR
210 220
LVHVFSLPLK YLTRSYVVYR KRS
Length:223
Mass (Da):25,296
Last modified:November 1, 1995 - v1
Checksum:i9C4379C5DF780000
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49884 Genomic DNA Translation: CAA90048.1
AL009126 Genomic DNA Translation: CAB15753.1
X85014 Genomic DNA Translation: CAA59374.1
X91819 Genomic DNA Translation: CAA62929.1
PIRiS60088
RefSeqiNP_391606.1, NC_000964.3
WP_003242665.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB15753; CAB15753; BSU37250
GeneIDi938454
KEGGibsu:BSU37250
PATRICifig|224308.179.peg.4036

Similar proteinsi

Entry informationi

Entry nameiNARI_BACSU
AccessioniPrimary (citable) accession number: P42177
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health