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P42176

- NARH_BACSU

UniProt

P42176 - NARH_BACSU

Protein

Nitrate reductase beta chain

Gene

narH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 3 4Fe-4S clusters per subunit.By similarity
    Binds 1 3Fe-4S cluster per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi16 – 161Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi19 – 191Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi22 – 221Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi26 – 261Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi181 – 1811Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi184 – 1841Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi189 – 1891Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi193 – 1931Iron-sulfur 4 (3Fe-4S)By similarity
    Metal bindingi214 – 2141Iron-sulfur 4 (3Fe-4S)By similarity
    Metal bindingi220 – 2201Iron-sulfur 4 (3Fe-4S)By similarity
    Metal bindingi224 – 2241Iron-sulfur 3 (4Fe-4S)By similarity
    Metal bindingi241 – 2411Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi244 – 2441Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi256 – 2561Iron-sulfur 2 (4Fe-4S)By similarity
    Metal bindingi260 – 2601Iron-sulfur 1 (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. metal ion binding Source: UniProtKB-KW
    4. nitrate reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

    Enzyme and pathway databases

    BioCyciBSUB:BSU37270-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrate reductase beta chain (EC:1.7.99.4)
    Gene namesi
    Name:narH
    Ordered Locus Names:BSU37270
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU37270. [Micado]

    Subcellular locationi

    Cell membrane By similarity; Peripheral membrane protein By similarity

    GO - Cellular componenti

    1. nitrate reductase complex Source: InterPro
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 487487Nitrate reductase beta chainPRO_0000096719Add
    BLAST

    Proteomic databases

    PaxDbiP42176.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU37270.

    Structurei

    3D structure databases

    ProteinModelPortaliP42176.
    SMRiP42176. Positions 1-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini7 – 36304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini172 – 203324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini205 – 234304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1140.
    HOGENOMiHOG000237353.
    KOiK00371.
    OMAiPHAWEDQ.
    OrthoDBiEOG6X3W4C.
    PhylomeDBiP42176.

    Family and domain databases

    Gene3Di1.10.3650.10. 1 hit.
    InterProiIPR017896. 4Fe4S_Fe-S-bd.
    IPR029263. Nitr_red_bet_C.
    IPR006547. NO3_Rdtase_bsu.
    [Graphical view]
    PfamiPF13247. Fer4_11. 1 hit.
    PF14711. Nitr_red_bet_C. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01660. narH. 1 hit.
    PROSITEiPS51379. 4FE4S_FER_2. 3 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42176-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKIKAQIGMV MNLDKCIGCH TCSVTCKNTW TNRSGAEYMY FNNVETKPGI    50
    GYPKQWEDQD KYKGGWTLKK GKLELKSGSK TNRLAGLFYN PNQPSIDDYY 100
    EPWNYDYETL TNSPQKKHQP VARPKSSLTG DFMNIEWGPN WEDDLAGGHI 150
    TGLEDPNVQK MEESIKTEFD DVFMMYLPRI CEHCINPACV SSCPSGAMYK 200
    REEDGIVLVD QNACRSWRYC VSSCPYKKVY FNWQTNKAEK CTLCFPRLEA 250
    GLPTICSETC VGRIRYLGVM LYDADKVEEA ASVENEKDLY HSQLDVFLDP 300
    NDPEVAKLAK EQGIPAEWIE AAQQSPIYKM IIDWKIALPL HPEYRTLPMV 350
    WYIPPLSPIM NLFEGKGSRQ TAEDIFPAID QMRIPIDYLA QLLTAGDTDH 400
    IRSTLKKMSV MRQYMRAVQT NKSIDPELIS SVGLTEQQIE DMYRLLAIAK 450
    YDDRFVIPSS HREEVSDLYA EQGSCGLSFS GGPGSCF 487
    Length:487
    Mass (Da):55,472
    Last modified:November 1, 1995 - v1
    Checksum:i7BCE6F32CC4F4283
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti21 – 211T → A in CAA62927. (PubMed:7557333)Curated
    Sequence conflicti21 – 211T → A in CAA59372. (PubMed:7557333)Curated
    Sequence conflicti174 – 1741M → I in CAA62927. (PubMed:7557333)Curated
    Sequence conflicti174 – 1741M → I in CAA59372. (PubMed:7557333)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49884 Genomic DNA. Translation: CAA90046.1.
    X91819 Genomic DNA. Translation: CAA62927.1.
    X85014 Genomic DNA. Translation: CAA59372.1.
    AL009126 Genomic DNA. Translation: CAB15755.1.
    PIRiS60086. S60573.
    RefSeqiNP_391608.1. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15755; CAB15755; BSU37270.
    GeneIDi937047.
    KEGGibsu:BSU37270.
    PATRICi18979492. VBIBacSub10457_3908.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49884 Genomic DNA. Translation: CAA90046.1 .
    X91819 Genomic DNA. Translation: CAA62927.1 .
    X85014 Genomic DNA. Translation: CAA59372.1 .
    AL009126 Genomic DNA. Translation: CAB15755.1 .
    PIRi S60086. S60573.
    RefSeqi NP_391608.1. NC_000964.3.

    3D structure databases

    ProteinModelPortali P42176.
    SMRi P42176. Positions 1-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU37270.

    Proteomic databases

    PaxDbi P42176.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15755 ; CAB15755 ; BSU37270 .
    GeneIDi 937047.
    KEGGi bsu:BSU37270.
    PATRICi 18979492. VBIBacSub10457_3908.

    Organism-specific databases

    GenoListi BSU37270. [Micado ]

    Phylogenomic databases

    eggNOGi COG1140.
    HOGENOMi HOG000237353.
    KOi K00371.
    OMAi PHAWEDQ.
    OrthoDBi EOG6X3W4C.
    PhylomeDBi P42176.

    Enzyme and pathway databases

    BioCyci BSUB:BSU37270-MONOMER.

    Family and domain databases

    Gene3Di 1.10.3650.10. 1 hit.
    InterProi IPR017896. 4Fe4S_Fe-S-bd.
    IPR029263. Nitr_red_bet_C.
    IPR006547. NO3_Rdtase_bsu.
    [Graphical view ]
    Pfami PF13247. Fer4_11. 1 hit.
    PF14711. Nitr_red_bet_C. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01660. narH. 1 hit.
    PROSITEi PS51379. 4FE4S_FER_2. 3 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
      Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
      EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
      Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
      Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
      Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
      FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.

    Entry informationi

    Entry nameiNARH_BACSU
    AccessioniPrimary (citable) accession number: P42176
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 108 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3