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Protein

Nitrate reductase beta chain

Gene

narH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterBy similarityNote: Binds 3 [4Fe-4S] clusters per subunit.By similarity
  • [3Fe-4S] clusterBy similarityNote: Binds 1 [3Fe-4S] cluster per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi19 – 191Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi22 – 221Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi26 – 261Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi181 – 1811Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi184 – 1841Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi189 – 1891Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi193 – 1931Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi214 – 2141Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi220 – 2201Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi224 – 2241Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi241 – 2411Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi244 – 2441Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi256 – 2561Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi260 – 2601Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU37270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase beta chain (EC:1.7.99.4)
Gene namesi
Name:narH
Ordered Locus Names:BSU37270
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570 Componenti: Chromosome

Organism-specific databases

GenoListiBSU37270. [Micado]

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Nitrate reductase beta chainPRO_0000096719Add
BLAST

Proteomic databases

PaxDbiP42176.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020146.

Structurei

3D structure databases

ProteinModelPortaliP42176.
SMRiP42176. Positions 1-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 36304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini172 – 203324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini205 – 234304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1140.
HOGENOMiHOG000237353.
InParanoidiP42176.
KOiK00371.
OMAiQSPIYKM.
OrthoDBiEOG6X3W4C.
PhylomeDBiP42176.

Family and domain databases

Gene3Di1.10.3650.10. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR029263. Nitr_red_bet_C.
IPR006547. NO3_Rdtase_bsu.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
PF14711. Nitr_red_bet_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01660. narH. 1 hit.
PROSITEiPS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42176-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIKAQIGMV MNLDKCIGCH TCSVTCKNTW TNRSGAEYMY FNNVETKPGI
60 70 80 90 100
GYPKQWEDQD KYKGGWTLKK GKLELKSGSK TNRLAGLFYN PNQPSIDDYY
110 120 130 140 150
EPWNYDYETL TNSPQKKHQP VARPKSSLTG DFMNIEWGPN WEDDLAGGHI
160 170 180 190 200
TGLEDPNVQK MEESIKTEFD DVFMMYLPRI CEHCINPACV SSCPSGAMYK
210 220 230 240 250
REEDGIVLVD QNACRSWRYC VSSCPYKKVY FNWQTNKAEK CTLCFPRLEA
260 270 280 290 300
GLPTICSETC VGRIRYLGVM LYDADKVEEA ASVENEKDLY HSQLDVFLDP
310 320 330 340 350
NDPEVAKLAK EQGIPAEWIE AAQQSPIYKM IIDWKIALPL HPEYRTLPMV
360 370 380 390 400
WYIPPLSPIM NLFEGKGSRQ TAEDIFPAID QMRIPIDYLA QLLTAGDTDH
410 420 430 440 450
IRSTLKKMSV MRQYMRAVQT NKSIDPELIS SVGLTEQQIE DMYRLLAIAK
460 470 480
YDDRFVIPSS HREEVSDLYA EQGSCGLSFS GGPGSCF
Length:487
Mass (Da):55,472
Last modified:November 1, 1995 - v1
Checksum:i7BCE6F32CC4F4283
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211T → A in CAA62927 (PubMed:7557333).Curated
Sequence conflicti21 – 211T → A in CAA59372 (PubMed:7557333).Curated
Sequence conflicti174 – 1741M → I in CAA62927 (PubMed:7557333).Curated
Sequence conflicti174 – 1741M → I in CAA59372 (PubMed:7557333).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49884 Genomic DNA. Translation: CAA90046.1.
X91819 Genomic DNA. Translation: CAA62927.1.
X85014 Genomic DNA. Translation: CAA59372.1.
AL009126 Genomic DNA. Translation: CAB15755.1.
PIRiS60086. S60573.
RefSeqiNP_391608.1. NC_000964.3.
WP_003244173.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15755; CAB15755; BSU37270.
GeneIDi937047.
KEGGibsu:BSU37270.
PATRICi18979492. VBIBacSub10457_3908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49884 Genomic DNA. Translation: CAA90046.1.
X91819 Genomic DNA. Translation: CAA62927.1.
X85014 Genomic DNA. Translation: CAA59372.1.
AL009126 Genomic DNA. Translation: CAB15755.1.
PIRiS60086. S60573.
RefSeqiNP_391608.1. NC_000964.3.
WP_003244173.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP42176.
SMRiP42176. Positions 1-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020146.

Proteomic databases

PaxDbiP42176.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15755; CAB15755; BSU37270.
GeneIDi937047.
KEGGibsu:BSU37270.
PATRICi18979492. VBIBacSub10457_3908.

Organism-specific databases

GenoListiBSU37270. [Micado]

Phylogenomic databases

eggNOGiCOG1140.
HOGENOMiHOG000237353.
InParanoidiP42176.
KOiK00371.
OMAiQSPIYKM.
OrthoDBiEOG6X3W4C.
PhylomeDBiP42176.

Enzyme and pathway databases

BioCyciBSUB:BSU37270-MONOMER.

Family and domain databases

Gene3Di1.10.3650.10. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR029263. Nitr_red_bet_C.
IPR006547. NO3_Rdtase_bsu.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
PF14711. Nitr_red_bet_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01660. narH. 1 hit.
PROSITEiPS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
    Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
    EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
    Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
    FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiNARH_BACSU
AccessioniPrimary (citable) accession number: P42176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.