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P42176 (NARH_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrate reductase beta chain

EC=1.7.99.4
Gene names
Name:narH
Ordered Locus Names:BSU37270
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length487 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 3 4Fe-4S clusters per subunit By similarity.

Binds 1 3Fe-4S cluster per subunit By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein By similarity.

Sequence similarities

Contains 3 4Fe-4S ferredoxin-type domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 487487Nitrate reductase beta chain
PRO_0000096719

Regions

Domain7 – 36304Fe-4S ferredoxin-type 1
Domain172 – 203324Fe-4S ferredoxin-type 2
Domain205 – 234304Fe-4S ferredoxin-type 3

Sites

Metal binding161Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding191Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding221Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding261Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding1811Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1841Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1891Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding1931Iron-sulfur 4 (3Fe-4S) By similarity
Metal binding2141Iron-sulfur 4 (3Fe-4S) By similarity
Metal binding2201Iron-sulfur 4 (3Fe-4S) By similarity
Metal binding2241Iron-sulfur 3 (4Fe-4S) By similarity
Metal binding2411Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2441Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2561Iron-sulfur 2 (4Fe-4S) By similarity
Metal binding2601Iron-sulfur 1 (4Fe-4S) By similarity

Experimental info

Sequence conflict211T → A in CAA62927. Ref.3
Sequence conflict211T → A in CAA59372. Ref.3
Sequence conflict1741M → I in CAA62927. Ref.3
Sequence conflict1741M → I in CAA59372. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P42176 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7BCE6F32CC4F4283

FASTA48755,472
        10         20         30         40         50         60 
MKIKAQIGMV MNLDKCIGCH TCSVTCKNTW TNRSGAEYMY FNNVETKPGI GYPKQWEDQD 

        70         80         90        100        110        120 
KYKGGWTLKK GKLELKSGSK TNRLAGLFYN PNQPSIDDYY EPWNYDYETL TNSPQKKHQP 

       130        140        150        160        170        180 
VARPKSSLTG DFMNIEWGPN WEDDLAGGHI TGLEDPNVQK MEESIKTEFD DVFMMYLPRI 

       190        200        210        220        230        240 
CEHCINPACV SSCPSGAMYK REEDGIVLVD QNACRSWRYC VSSCPYKKVY FNWQTNKAEK 

       250        260        270        280        290        300 
CTLCFPRLEA GLPTICSETC VGRIRYLGVM LYDADKVEEA ASVENEKDLY HSQLDVFLDP 

       310        320        330        340        350        360 
NDPEVAKLAK EQGIPAEWIE AAQQSPIYKM IIDWKIALPL HPEYRTLPMV WYIPPLSPIM 

       370        380        390        400        410        420 
NLFEGKGSRQ TAEDIFPAID QMRIPIDYLA QLLTAGDTDH IRSTLKKMSV MRQYMRAVQT 

       430        440        450        460        470        480 
NKSIDPELIS SVGLTEQQIE DMYRLLAIAK YDDRFVIPSS HREEVSDLYA EQGSCGLSFS 


GGPGSCF 

« Hide

References

« Hide 'large scale' references
[1]"Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[4]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49884 Genomic DNA. Translation: CAA90046.1.
X91819 Genomic DNA. Translation: CAA62927.1.
X85014 Genomic DNA. Translation: CAA59372.1.
AL009126 Genomic DNA. Translation: CAB15755.1.
PIRS60573. S60086.
RefSeqNP_391608.1. NC_000964.3.

3D structure databases

ProteinModelPortalP42176.
SMRP42176. Positions 1-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU37270.

Proteomic databases

PaxDbP42176.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15755; CAB15755; BSU37270.
GeneID937047.
KEGGbsu:BSU37270.
PATRIC18979492. VBIBacSub10457_3908.

Organism-specific databases

GenoListBSU37270. [Micado]

Phylogenomic databases

eggNOGCOG1140.
HOGENOMHOG000237353.
KOK00371.
OMAPHAWEDQ.
OrthoDBEOG6X3W4C.
PhylomeDBP42176.

Enzyme and pathway databases

BioCycBSUB:BSU37270-MONOMER.

Family and domain databases

Gene3D1.10.3650.10. 1 hit.
InterProIPR017896. 4Fe4S_Fe-S-bd.
IPR029263. Nitr_red_bet_C.
IPR006547. NO3_Rdtase_bsu.
[Graphical view]
PfamPF13247. Fer4_11. 1 hit.
PF14711. Nitr_red_bet_C. 1 hit.
[Graphical view]
TIGRFAMsTIGR01660. narH. 1 hit.
PROSITEPS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNARH_BACSU
AccessionPrimary (citable) accession number: P42176
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList