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P42176

- NARH_BACSU

UniProt

P42176 - NARH_BACSU

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Protein

Nitrate reductase beta chain

Gene

narH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 3 4Fe-4S clusters per subunit.By similarity
Binds 1 3Fe-4S cluster per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi19 – 191Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi22 – 221Iron-sulfur 1 (4Fe-4S)By similarity
Metal bindingi26 – 261Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi181 – 1811Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi184 – 1841Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi189 – 1891Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi193 – 1931Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi214 – 2141Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi220 – 2201Iron-sulfur 4 (3Fe-4S)By similarity
Metal bindingi224 – 2241Iron-sulfur 3 (4Fe-4S)By similarity
Metal bindingi241 – 2411Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi244 – 2441Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi256 – 2561Iron-sulfur 2 (4Fe-4S)By similarity
Metal bindingi260 – 2601Iron-sulfur 1 (4Fe-4S)By similarity

GO - Molecular functioni

  1. 3 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW
  4. nitrate reductase activity Source: UniProtKB-EC

GO - Biological processi

  1. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

3Fe-4S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU37270-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase beta chain (EC:1.7.99.4)
Gene namesi
Name:narH
Ordered Locus Names:BSU37270
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU37270. [Micado]

Subcellular locationi

Cell membrane By similarity; Peripheral membrane protein By similarity

GO - Cellular componenti

  1. nitrate reductase complex Source: InterPro
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 487487Nitrate reductase beta chainPRO_0000096719Add
BLAST

Proteomic databases

PaxDbiP42176.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU37270.

Structurei

3D structure databases

ProteinModelPortaliP42176.
SMRiP42176. Positions 1-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 36304Fe-4S ferredoxin-type 1PROSITE-ProRule annotationAdd
BLAST
Domaini172 – 203324Fe-4S ferredoxin-type 2PROSITE-ProRule annotationAdd
BLAST
Domaini205 – 234304Fe-4S ferredoxin-type 3PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 3 4Fe-4S ferredoxin-type domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1140.
HOGENOMiHOG000237353.
InParanoidiP42176.
KOiK00371.
OMAiPHAWEDQ.
OrthoDBiEOG6X3W4C.
PhylomeDBiP42176.

Family and domain databases

Gene3Di1.10.3650.10. 1 hit.
InterProiIPR017896. 4Fe4S_Fe-S-bd.
IPR029263. Nitr_red_bet_C.
IPR006547. NO3_Rdtase_bsu.
[Graphical view]
PfamiPF13247. Fer4_11. 1 hit.
PF14711. Nitr_red_bet_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01660. narH. 1 hit.
PROSITEiPS51379. 4FE4S_FER_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42176-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIKAQIGMV MNLDKCIGCH TCSVTCKNTW TNRSGAEYMY FNNVETKPGI
60 70 80 90 100
GYPKQWEDQD KYKGGWTLKK GKLELKSGSK TNRLAGLFYN PNQPSIDDYY
110 120 130 140 150
EPWNYDYETL TNSPQKKHQP VARPKSSLTG DFMNIEWGPN WEDDLAGGHI
160 170 180 190 200
TGLEDPNVQK MEESIKTEFD DVFMMYLPRI CEHCINPACV SSCPSGAMYK
210 220 230 240 250
REEDGIVLVD QNACRSWRYC VSSCPYKKVY FNWQTNKAEK CTLCFPRLEA
260 270 280 290 300
GLPTICSETC VGRIRYLGVM LYDADKVEEA ASVENEKDLY HSQLDVFLDP
310 320 330 340 350
NDPEVAKLAK EQGIPAEWIE AAQQSPIYKM IIDWKIALPL HPEYRTLPMV
360 370 380 390 400
WYIPPLSPIM NLFEGKGSRQ TAEDIFPAID QMRIPIDYLA QLLTAGDTDH
410 420 430 440 450
IRSTLKKMSV MRQYMRAVQT NKSIDPELIS SVGLTEQQIE DMYRLLAIAK
460 470 480
YDDRFVIPSS HREEVSDLYA EQGSCGLSFS GGPGSCF
Length:487
Mass (Da):55,472
Last modified:November 1, 1995 - v1
Checksum:i7BCE6F32CC4F4283
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti21 – 211T → A in CAA62927. (PubMed:7557333)Curated
Sequence conflicti21 – 211T → A in CAA59372. (PubMed:7557333)Curated
Sequence conflicti174 – 1741M → I in CAA62927. (PubMed:7557333)Curated
Sequence conflicti174 – 1741M → I in CAA59372. (PubMed:7557333)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49884 Genomic DNA. Translation: CAA90046.1.
X91819 Genomic DNA. Translation: CAA62927.1.
X85014 Genomic DNA. Translation: CAA59372.1.
AL009126 Genomic DNA. Translation: CAB15755.1.
PIRiS60086. S60573.
RefSeqiNP_391608.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15755; CAB15755; BSU37270.
GeneIDi937047.
KEGGibsu:BSU37270.
PATRICi18979492. VBIBacSub10457_3908.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49884 Genomic DNA. Translation: CAA90046.1 .
X91819 Genomic DNA. Translation: CAA62927.1 .
X85014 Genomic DNA. Translation: CAA59372.1 .
AL009126 Genomic DNA. Translation: CAB15755.1 .
PIRi S60086. S60573.
RefSeqi NP_391608.1. NC_000964.3.

3D structure databases

ProteinModelPortali P42176.
SMRi P42176. Positions 1-463.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU37270.

Proteomic databases

PaxDbi P42176.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15755 ; CAB15755 ; BSU37270 .
GeneIDi 937047.
KEGGi bsu:BSU37270.
PATRICi 18979492. VBIBacSub10457_3908.

Organism-specific databases

GenoListi BSU37270. [Micado ]

Phylogenomic databases

eggNOGi COG1140.
HOGENOMi HOG000237353.
InParanoidi P42176.
KOi K00371.
OMAi PHAWEDQ.
OrthoDBi EOG6X3W4C.
PhylomeDBi P42176.

Enzyme and pathway databases

BioCyci BSUB:BSU37270-MONOMER.

Family and domain databases

Gene3Di 1.10.3650.10. 1 hit.
InterProi IPR017896. 4Fe4S_Fe-S-bd.
IPR029263. Nitr_red_bet_C.
IPR006547. NO3_Rdtase_bsu.
[Graphical view ]
Pfami PF13247. Fer4_11. 1 hit.
PF14711. Nitr_red_bet_C. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01660. narH. 1 hit.
PROSITEi PS51379. 4FE4S_FER_2. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
    Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
    EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
    Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
    FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.

Entry informationi

Entry nameiNARH_BACSU
AccessioniPrimary (citable) accession number: P42176
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3