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P42175

- NARG_BACSU

UniProt

P42175 - NARG_BACSU

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Protein

Nitrate reductase alpha chain

Gene

narG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

The alpha chain is the actual site of nitrate reduction.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Iron-sulfur (4Fe-4S); via pros nitrogenPROSITE-ProRule annotation
Metal bindingi58 – 581Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi62 – 621Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi97 – 971Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
Metal bindingi227 – 2271MolybdenumBy similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. molybdenum ion binding Source: InterPro
  4. nitrate reductase activity Source: UniProtKB-EC
  5. oxidoreductase activity, acting on NAD(P)H Source: RefGenome

GO - Biological processi

  1. cellular respiration Source: RefGenome
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBSUB:BSU37280-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase alpha chain (EC:1.7.99.4)
Gene namesi
Name:narG
Ordered Locus Names:BSU37280
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU37280. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. nitrate reductase complex Source: InterPro
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12281228Nitrate reductase alpha chainPRO_0000063232Add
BLAST

Proteomic databases

PaxDbiP42175.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU37280.

Structurei

3D structure databases

ProteinModelPortaliP42175.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 111654Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5013.
HOGENOMiHOG000237341.
InParanoidiP42175.
KOiK00370.
OrthoDBiEOG6RC3JS.
PhylomeDBiP42175.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01580. narG. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42175-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKKKRSPLF RRLNYFSPIE HHSNKHSQTT REDRDWENVY RNRWQYDKVV
60 70 80 90 100
RSTHGVNCTG SCSWNIYVKN GIVTWEGQNL NYPSTGPDMP DFEPRGCPRG
110 120 130 140 150
ASFSWYIYSP LRVKYPYVRG VLINLWREAL QTHQNPLEAW KSIVENPEKA
160 170 180 190 200
KSYKQARGKG GFVRAEWPEV LKLISASLLY TVMKYGPDRN VGFSPIPAMS
210 220 230 240 250
MISHASGSRF MSLIGGPMLS FYDWYADLPP ASPQIWGDQT DVPESSDWYN
260 270 280 290 300
SGYIITWGSN VPLTRTPDAH FLAEARYKGA KVISISPDFA ESSKFADDWL
310 320 330 340 350
SIRQGTDGAL AMAMGHVILQ EFYVNQETER FIEYAKQYTD FPFLVTLSKE
360 370 380 390 400
NGVYTAGRFL HAKDIGRKTK HDQWKPAVWD EQTSSFAIPQ GTMGSRWDGQ
410 420 430 440 450
QKWNLHMIDE ETGEPIEPRL SVLGIEDEIG TVRIPYFSND GNKVLERDLP
460 470 480 490 500
IKKMNLNGEE TYITTVFDLI LANYGVNRGI GERSAVSYDD PEPFTPAWQE
510 520 530 540 550
QMTGIKKEAV VKIAREFAQN AIDTDGRSMI IVGAGINHWF NSDTIYRAVL
560 570 580 590 600
NLVLLVGAQG VNGGGWAHYV GQEKLRPAEG WQTIATAKDW EGVPKLQNGT
610 620 630 640 650
SFFYFATDQW RYEDQPISDL ASPIAASSRY KHHADYNVLA ARLGWLPSYP
660 670 680 690 700
TFNQNGIDLY KEAEKAGAAT PEDVGAYVAS QLQEKKLKFA IEDPDNEVNF
710 720 730 740 750
PRNLFVWRAN LISSSGKGHE YFLKHLLGTT NGLMNDDSDS IRPEEIKWRE
760 770 780 790 800
QAPEGKLDLL INLDFRMAGT ALYSDIVLPA ATWYEKHDLS STDMHPFIHP
810 820 830 840 850
FAPAISAPWE SKSDWDIFKA LSKAVSDLAE EVDMEPVKEV VATPLLHDTM
860 870 880 890 900
QELAQPFGKI NDWSKGECEA IPGKTMPNIQ VVERDYKHIF HKMTALGPNV
910 920 930 940 950
ALKPSGTKGM SWSIADEYES LKQRLGEITS DSVAKGCPNI SEAKQAAEAI
960 970 980 990 1000
LTLSSTSNGK VAVKAWESLE NITNLKLKDL AEEREEECFT FEQITAQPKT
1010 1020 1030 1040 1050
VITSPAFTGS EKGGRRYSPF TTNVEKLIPW RTLTGRQSYY VDHELMMEFG
1060 1070 1080 1090 1100
ETMATFKPIL QHRPFLSKRP DQEGKEIVLN YLTPHNKWSV HSMYFDSLPM
1110 1120 1130 1140 1150
LTLFRGGPTV WMNKDDAEDT DIKDNDWIEC FNRNGVVVAR AVLSHRIPKG
1160 1170 1180 1190 1200
MAFMHHAQDR HINVPGTKLT NNRGGTHNSP TRIHVKPTQM IGGYAQLSYG
1210 1220
FNYYGPTGNQ RDLNVVIRKL KEVDWLED
Length:1,228
Mass (Da):139,099
Last modified:July 28, 2009 - v2
Checksum:i60E6B3A83422CBF8
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471D → T in CAA90045. (PubMed:8846791)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49884 Genomic DNA. Translation: CAA90045.1.
X91819 Genomic DNA. Translation: CAA62926.1.
X85014 Genomic DNA. Translation: CAA59371.1.
AL009126 Genomic DNA. Translation: CAB15756.2.
PIRiS60085.
RefSeqiNP_391609.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15756; CAB15756; BSU37280.
GeneIDi938358.
KEGGibsu:BSU37280.
PATRICi18979494. VBIBacSub10457_3909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49884 Genomic DNA. Translation: CAA90045.1 .
X91819 Genomic DNA. Translation: CAA62926.1 .
X85014 Genomic DNA. Translation: CAA59371.1 .
AL009126 Genomic DNA. Translation: CAB15756.2 .
PIRi S60085.
RefSeqi NP_391609.2. NC_000964.3.

3D structure databases

ProteinModelPortali P42175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU37280.

Proteomic databases

PaxDbi P42175.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15756 ; CAB15756 ; BSU37280 .
GeneIDi 938358.
KEGGi bsu:BSU37280.
PATRICi 18979494. VBIBacSub10457_3909.

Organism-specific databases

GenoListi BSU37280. [Micado ]

Phylogenomic databases

eggNOGi COG5013.
HOGENOMi HOG000237341.
InParanoidi P42175.
KOi K00370.
OrthoDBi EOG6RC3JS.
PhylomeDBi P42175.

Enzyme and pathway databases

BioCyci BSUB:BSU37280-MONOMER.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view ]
Pfami PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01580. narG. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
    Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
    EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
    Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
    FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 47.

Entry informationi

Entry nameiNARG_BACSU
AccessioniPrimary (citable) accession number: P42175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 28, 2009
Last modified: November 26, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3