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P42175

- NARG_BACSU

UniProt

P42175 - NARG_BACSU

Protein

Nitrate reductase alpha chain

Gene

narG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 2 (28 Jul 2009)
      Previous versions | rss
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    Functioni

    The alpha chain is the actual site of nitrate reduction.

    Catalytic activityi

    Nitrite + acceptor = nitrate + reduced acceptor.

    Cofactori

    Binds 1 4Fe-4S cluster per subunit.By similarity
    Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi54 – 541Iron-sulfur (4Fe-4S); via pros nitrogenPROSITE-ProRule annotation
    Metal bindingi58 – 581Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi62 – 621Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi97 – 971Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation
    Metal bindingi227 – 2271MolybdenumBy similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. electron carrier activity Source: InterPro
    3. molybdenum ion binding Source: InterPro
    4. nitrate reductase activity Source: UniProtKB-EC
    5. oxidoreductase activity, acting on NAD(P)H Source: RefGenome

    GO - Biological processi

    1. cellular respiration Source: RefGenome
    2. nitrate assimilation Source: UniProtKB-KW

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Electron transport, Nitrate assimilation, Transport

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

    Enzyme and pathway databases

    BioCyciBSUB:BSU37280-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrate reductase alpha chain (EC:1.7.99.4)
    Gene namesi
    Name:narG
    Ordered Locus Names:BSU37280
    OrganismiBacillus subtilis (strain 168)
    Taxonomic identifieri224308 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
    ProteomesiUP000001570: Chromosome

    Organism-specific databases

    GenoListiBSU37280. [Micado]

    Subcellular locationi

    GO - Cellular componenti

    1. nitrate reductase complex Source: InterPro
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 12281228Nitrate reductase alpha chainPRO_0000063232Add
    BLAST

    Proteomic databases

    PaxDbiP42175.

    Interactioni

    Protein-protein interaction databases

    STRINGi224308.BSU37280.

    Structurei

    3D structure databases

    ProteinModelPortaliP42175.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini47 – 111654Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 4Fe-4S Mo/W bis-MGD-type domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5013.
    HOGENOMiHOG000237341.
    KOiK00370.
    OrthoDBiEOG6RC3JS.
    PhylomeDBiP42175.

    Family and domain databases

    InterProiIPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR006468. NarG.
    IPR028189. Nitr_red_alph_N.
    [Graphical view]
    PfamiPF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF14710. Nitr_red_alph_N. 1 hit.
    [Graphical view]
    SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view]
    SUPFAMiSSF50692. SSF50692. 1 hit.
    TIGRFAMsiTIGR01580. narG. 1 hit.
    PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P42175-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKKRSPLF RRLNYFSPIE HHSNKHSQTT REDRDWENVY RNRWQYDKVV     50
    RSTHGVNCTG SCSWNIYVKN GIVTWEGQNL NYPSTGPDMP DFEPRGCPRG 100
    ASFSWYIYSP LRVKYPYVRG VLINLWREAL QTHQNPLEAW KSIVENPEKA 150
    KSYKQARGKG GFVRAEWPEV LKLISASLLY TVMKYGPDRN VGFSPIPAMS 200
    MISHASGSRF MSLIGGPMLS FYDWYADLPP ASPQIWGDQT DVPESSDWYN 250
    SGYIITWGSN VPLTRTPDAH FLAEARYKGA KVISISPDFA ESSKFADDWL 300
    SIRQGTDGAL AMAMGHVILQ EFYVNQETER FIEYAKQYTD FPFLVTLSKE 350
    NGVYTAGRFL HAKDIGRKTK HDQWKPAVWD EQTSSFAIPQ GTMGSRWDGQ 400
    QKWNLHMIDE ETGEPIEPRL SVLGIEDEIG TVRIPYFSND GNKVLERDLP 450
    IKKMNLNGEE TYITTVFDLI LANYGVNRGI GERSAVSYDD PEPFTPAWQE 500
    QMTGIKKEAV VKIAREFAQN AIDTDGRSMI IVGAGINHWF NSDTIYRAVL 550
    NLVLLVGAQG VNGGGWAHYV GQEKLRPAEG WQTIATAKDW EGVPKLQNGT 600
    SFFYFATDQW RYEDQPISDL ASPIAASSRY KHHADYNVLA ARLGWLPSYP 650
    TFNQNGIDLY KEAEKAGAAT PEDVGAYVAS QLQEKKLKFA IEDPDNEVNF 700
    PRNLFVWRAN LISSSGKGHE YFLKHLLGTT NGLMNDDSDS IRPEEIKWRE 750
    QAPEGKLDLL INLDFRMAGT ALYSDIVLPA ATWYEKHDLS STDMHPFIHP 800
    FAPAISAPWE SKSDWDIFKA LSKAVSDLAE EVDMEPVKEV VATPLLHDTM 850
    QELAQPFGKI NDWSKGECEA IPGKTMPNIQ VVERDYKHIF HKMTALGPNV 900
    ALKPSGTKGM SWSIADEYES LKQRLGEITS DSVAKGCPNI SEAKQAAEAI 950
    LTLSSTSNGK VAVKAWESLE NITNLKLKDL AEEREEECFT FEQITAQPKT 1000
    VITSPAFTGS EKGGRRYSPF TTNVEKLIPW RTLTGRQSYY VDHELMMEFG 1050
    ETMATFKPIL QHRPFLSKRP DQEGKEIVLN YLTPHNKWSV HSMYFDSLPM 1100
    LTLFRGGPTV WMNKDDAEDT DIKDNDWIEC FNRNGVVVAR AVLSHRIPKG 1150
    MAFMHHAQDR HINVPGTKLT NNRGGTHNSP TRIHVKPTQM IGGYAQLSYG 1200
    FNYYGPTGNQ RDLNVVIRKL KEVDWLED 1228
    Length:1,228
    Mass (Da):139,099
    Last modified:July 28, 2009 - v2
    Checksum:i60E6B3A83422CBF8
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti47 – 471D → T in CAA90045. (PubMed:8846791)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49884 Genomic DNA. Translation: CAA90045.1.
    X91819 Genomic DNA. Translation: CAA62926.1.
    X85014 Genomic DNA. Translation: CAA59371.1.
    AL009126 Genomic DNA. Translation: CAB15756.2.
    PIRiS60085.
    RefSeqiNP_391609.2. NC_000964.3.

    Genome annotation databases

    EnsemblBacteriaiCAB15756; CAB15756; BSU37280.
    GeneIDi938358.
    KEGGibsu:BSU37280.
    PATRICi18979494. VBIBacSub10457_3909.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49884 Genomic DNA. Translation: CAA90045.1 .
    X91819 Genomic DNA. Translation: CAA62926.1 .
    X85014 Genomic DNA. Translation: CAA59371.1 .
    AL009126 Genomic DNA. Translation: CAB15756.2 .
    PIRi S60085.
    RefSeqi NP_391609.2. NC_000964.3.

    3D structure databases

    ProteinModelPortali P42175.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224308.BSU37280.

    Proteomic databases

    PaxDbi P42175.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai CAB15756 ; CAB15756 ; BSU37280 .
    GeneIDi 938358.
    KEGGi bsu:BSU37280.
    PATRICi 18979494. VBIBacSub10457_3909.

    Organism-specific databases

    GenoListi BSU37280. [Micado ]

    Phylogenomic databases

    eggNOGi COG5013.
    HOGENOMi HOG000237341.
    KOi K00370.
    OrthoDBi EOG6RC3JS.
    PhylomeDBi P42175.

    Enzyme and pathway databases

    BioCyci BSUB:BSU37280-MONOMER.

    Family and domain databases

    InterProi IPR009010. Asp_de-COase-like_dom.
    IPR006657. MoPterin_dinucl-bd_dom.
    IPR006656. Mopterin_OxRdtase.
    IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
    IPR006655. Mopterin_OxRdtase_prok_CS.
    IPR027467. MopterinOxRdtase_cofactor_BS.
    IPR006468. NarG.
    IPR028189. Nitr_red_alph_N.
    [Graphical view ]
    Pfami PF00384. Molybdopterin. 1 hit.
    PF01568. Molydop_binding. 1 hit.
    PF14710. Nitr_red_alph_N. 1 hit.
    [Graphical view ]
    SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50692. SSF50692. 1 hit.
    TIGRFAMsi TIGR01580. narG. 1 hit.
    PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
    PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
    PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
    PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
      Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
      EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
      Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
      Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168.
    3. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
      Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
      FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: 168 / JH642.
    4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
      Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
      , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
      Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 168.
    5. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
      Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
      Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: SEQUENCE REVISION TO 47.

    Entry informationi

    Entry nameiNARG_BACSU
    AccessioniPrimary (citable) accession number: P42175
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: July 28, 2009
    Last modified: October 1, 2014
    This is version 114 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Bacillus subtilis
      Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3