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Protein

Nitrate reductase alpha chain

Gene

narG

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

The alpha chain is the actual site of nitrate reduction.

Catalytic activityi

Nitrate + a quinol = nitrite + a quinone + H2O.

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi54Iron-sulfur (4Fe-4S); via pros nitrogenPROSITE-ProRule annotation1
Metal bindingi58Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi62Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi97Iron-sulfur (4Fe-4S)PROSITE-ProRule annotation1
Metal bindingi227MolybdenumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processElectron transport, Nitrate assimilation, Transport
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBSUB:BSU37280-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase alpha chain (EC:1.7.5.1)
Gene namesi
Name:narG
Ordered Locus Names:BSU37280
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000632321 – 1228Nitrate reductase alpha chainAdd BLAST1228

Proteomic databases

PaxDbiP42175
PRIDEiP42175

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100020151

Structurei

3D structure databases

ProteinModelPortaliP42175
SMRiP42175
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini47 – 1114Fe-4S Mo/W bis-MGD-typePROSITE-ProRule annotationAdd BLAST65

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5013 LUCA
HOGENOMiHOG000237341
InParanoidiP42175
KOiK00370
OMAiGAAMNHW
PhylomeDBiP42175

Family and domain databases

CDDicd02776 MopB_CT_Nitrate-R-NarG-like, 1 hit
InterProiView protein in InterPro
IPR009010 Asp_de-COase-like_dom_sf
IPR037943 MopB_CT_Nitrate-R-NarG-like
IPR006657 MoPterin_dinucl-bd_dom
IPR006656 Mopterin_OxRdtase
IPR006963 Mopterin_OxRdtase_4Fe-4S_dom
IPR006655 Mopterin_OxRdtase_prok_CS
IPR027467 MopterinOxRdtase_cofactor_BS
IPR006468 NarG
IPR028189 Nitr_red_alph_N
PfamiView protein in Pfam
PF00384 Molybdopterin, 1 hit
PF01568 Molydop_binding, 1 hit
PF14710 Nitr_red_alph_N, 1 hit
SMARTiView protein in SMART
SM00926 Molybdop_Fe4S4, 1 hit
SUPFAMiSSF50692 SSF50692, 1 hit
TIGRFAMsiTIGR01580 narG, 1 hit
PROSITEiView protein in PROSITE
PS51669 4FE4S_MOW_BIS_MGD, 1 hit
PS00551 MOLYBDOPTERIN_PROK_1, 1 hit
PS00490 MOLYBDOPTERIN_PROK_2, 1 hit
PS00932 MOLYBDOPTERIN_PROK_3, 1 hit

Sequencei

Sequence statusi: Complete.

P42175-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKKRSPLF RRLNYFSPIE HHSNKHSQTT REDRDWENVY RNRWQYDKVV
60 70 80 90 100
RSTHGVNCTG SCSWNIYVKN GIVTWEGQNL NYPSTGPDMP DFEPRGCPRG
110 120 130 140 150
ASFSWYIYSP LRVKYPYVRG VLINLWREAL QTHQNPLEAW KSIVENPEKA
160 170 180 190 200
KSYKQARGKG GFVRAEWPEV LKLISASLLY TVMKYGPDRN VGFSPIPAMS
210 220 230 240 250
MISHASGSRF MSLIGGPMLS FYDWYADLPP ASPQIWGDQT DVPESSDWYN
260 270 280 290 300
SGYIITWGSN VPLTRTPDAH FLAEARYKGA KVISISPDFA ESSKFADDWL
310 320 330 340 350
SIRQGTDGAL AMAMGHVILQ EFYVNQETER FIEYAKQYTD FPFLVTLSKE
360 370 380 390 400
NGVYTAGRFL HAKDIGRKTK HDQWKPAVWD EQTSSFAIPQ GTMGSRWDGQ
410 420 430 440 450
QKWNLHMIDE ETGEPIEPRL SVLGIEDEIG TVRIPYFSND GNKVLERDLP
460 470 480 490 500
IKKMNLNGEE TYITTVFDLI LANYGVNRGI GERSAVSYDD PEPFTPAWQE
510 520 530 540 550
QMTGIKKEAV VKIAREFAQN AIDTDGRSMI IVGAGINHWF NSDTIYRAVL
560 570 580 590 600
NLVLLVGAQG VNGGGWAHYV GQEKLRPAEG WQTIATAKDW EGVPKLQNGT
610 620 630 640 650
SFFYFATDQW RYEDQPISDL ASPIAASSRY KHHADYNVLA ARLGWLPSYP
660 670 680 690 700
TFNQNGIDLY KEAEKAGAAT PEDVGAYVAS QLQEKKLKFA IEDPDNEVNF
710 720 730 740 750
PRNLFVWRAN LISSSGKGHE YFLKHLLGTT NGLMNDDSDS IRPEEIKWRE
760 770 780 790 800
QAPEGKLDLL INLDFRMAGT ALYSDIVLPA ATWYEKHDLS STDMHPFIHP
810 820 830 840 850
FAPAISAPWE SKSDWDIFKA LSKAVSDLAE EVDMEPVKEV VATPLLHDTM
860 870 880 890 900
QELAQPFGKI NDWSKGECEA IPGKTMPNIQ VVERDYKHIF HKMTALGPNV
910 920 930 940 950
ALKPSGTKGM SWSIADEYES LKQRLGEITS DSVAKGCPNI SEAKQAAEAI
960 970 980 990 1000
LTLSSTSNGK VAVKAWESLE NITNLKLKDL AEEREEECFT FEQITAQPKT
1010 1020 1030 1040 1050
VITSPAFTGS EKGGRRYSPF TTNVEKLIPW RTLTGRQSYY VDHELMMEFG
1060 1070 1080 1090 1100
ETMATFKPIL QHRPFLSKRP DQEGKEIVLN YLTPHNKWSV HSMYFDSLPM
1110 1120 1130 1140 1150
LTLFRGGPTV WMNKDDAEDT DIKDNDWIEC FNRNGVVVAR AVLSHRIPKG
1160 1170 1180 1190 1200
MAFMHHAQDR HINVPGTKLT NNRGGTHNSP TRIHVKPTQM IGGYAQLSYG
1210 1220
FNYYGPTGNQ RDLNVVIRKL KEVDWLED
Length:1,228
Mass (Da):139,099
Last modified:July 28, 2009 - v2
Checksum:i60E6B3A83422CBF8
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti47D → T in CAA90045 (PubMed:8846791).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49884 Genomic DNA Translation: CAA90045.1
X91819 Genomic DNA Translation: CAA62926.1
X85014 Genomic DNA Translation: CAA59371.1
AL009126 Genomic DNA Translation: CAB15756.2
PIRiS60085
RefSeqiNP_391609.2, NC_000964.3
WP_003243085.1, NZ_JNCM01000034.1

Genome annotation databases

EnsemblBacteriaiCAB15756; CAB15756; BSU37280
GeneIDi938358
KEGGibsu:BSU37280
PATRICifig|224308.179.peg.4039

Similar proteinsi

Entry informationi

Entry nameiNARG_BACSU
AccessioniPrimary (citable) accession number: P42175
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 28, 2009
Last modified: April 25, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

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