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P42175

- NARG_BACSU

UniProt

P42175 - NARG_BACSU

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Protein

Nitrate reductase alpha chain

Gene
narG, BSU37280
Organism
Bacillus subtilis (strain 168)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

The alpha chain is the actual site of nitrate reduction.

Catalytic activityi

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactori

Binds 1 4Fe-4S cluster per subunit By similarity.
Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi54 – 541Iron-sulfur (4Fe-4S); via pros nitrogen By similarity
Metal bindingi58 – 581Iron-sulfur (4Fe-4S) By similarity
Metal bindingi62 – 621Iron-sulfur (4Fe-4S) By similarity
Metal bindingi97 – 971Iron-sulfur (4Fe-4S) By similarity
Metal bindingi227 – 2271Molybdenum By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. electron carrier activity Source: InterPro
  3. molybdenum ion binding Source: InterPro
  4. nitrate reductase activity Source: UniProtKB-EC
  5. oxidoreductase activity, acting on NAD(P)H Source: RefGenome

GO - Biological processi

  1. cellular respiration Source: RefGenome
  2. nitrate assimilation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Electron transport, Nitrate assimilation, Transport

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, Molybdenum

Enzyme and pathway databases

BioCyciBSUB:BSU37280-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Nitrate reductase alpha chain (EC:1.7.99.4)
Gene namesi
Name:narG
Ordered Locus Names:BSU37280
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
ProteomesiUP000001570: Chromosome

Organism-specific databases

GenoListiBSU37280. [Micado]

Subcellular locationi

GO - Cellular componenti

  1. nitrate reductase complex Source: InterPro
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12281228Nitrate reductase alpha chainPRO_0000063232Add
BLAST

Proteomic databases

PaxDbiP42175.

Interactioni

Protein-protein interaction databases

STRINGi224308.BSU37280.

Structurei

3D structure databases

ProteinModelPortaliP42175.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini47 – 111654Fe-4S Mo/W bis-MGD-typeAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5013.
HOGENOMiHOG000237341.
KOiK00370.
OrthoDBiEOG6RC3JS.
PhylomeDBiP42175.

Family and domain databases

InterProiIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamiPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SMARTiSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMiSSF50692. SSF50692. 1 hit.
TIGRFAMsiTIGR01580. narG. 1 hit.
PROSITEiPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42175-1 [UniParc]FASTAAdd to Basket

« Hide

MKKKKRSPLF RRLNYFSPIE HHSNKHSQTT REDRDWENVY RNRWQYDKVV     50
RSTHGVNCTG SCSWNIYVKN GIVTWEGQNL NYPSTGPDMP DFEPRGCPRG 100
ASFSWYIYSP LRVKYPYVRG VLINLWREAL QTHQNPLEAW KSIVENPEKA 150
KSYKQARGKG GFVRAEWPEV LKLISASLLY TVMKYGPDRN VGFSPIPAMS 200
MISHASGSRF MSLIGGPMLS FYDWYADLPP ASPQIWGDQT DVPESSDWYN 250
SGYIITWGSN VPLTRTPDAH FLAEARYKGA KVISISPDFA ESSKFADDWL 300
SIRQGTDGAL AMAMGHVILQ EFYVNQETER FIEYAKQYTD FPFLVTLSKE 350
NGVYTAGRFL HAKDIGRKTK HDQWKPAVWD EQTSSFAIPQ GTMGSRWDGQ 400
QKWNLHMIDE ETGEPIEPRL SVLGIEDEIG TVRIPYFSND GNKVLERDLP 450
IKKMNLNGEE TYITTVFDLI LANYGVNRGI GERSAVSYDD PEPFTPAWQE 500
QMTGIKKEAV VKIAREFAQN AIDTDGRSMI IVGAGINHWF NSDTIYRAVL 550
NLVLLVGAQG VNGGGWAHYV GQEKLRPAEG WQTIATAKDW EGVPKLQNGT 600
SFFYFATDQW RYEDQPISDL ASPIAASSRY KHHADYNVLA ARLGWLPSYP 650
TFNQNGIDLY KEAEKAGAAT PEDVGAYVAS QLQEKKLKFA IEDPDNEVNF 700
PRNLFVWRAN LISSSGKGHE YFLKHLLGTT NGLMNDDSDS IRPEEIKWRE 750
QAPEGKLDLL INLDFRMAGT ALYSDIVLPA ATWYEKHDLS STDMHPFIHP 800
FAPAISAPWE SKSDWDIFKA LSKAVSDLAE EVDMEPVKEV VATPLLHDTM 850
QELAQPFGKI NDWSKGECEA IPGKTMPNIQ VVERDYKHIF HKMTALGPNV 900
ALKPSGTKGM SWSIADEYES LKQRLGEITS DSVAKGCPNI SEAKQAAEAI 950
LTLSSTSNGK VAVKAWESLE NITNLKLKDL AEEREEECFT FEQITAQPKT 1000
VITSPAFTGS EKGGRRYSPF TTNVEKLIPW RTLTGRQSYY VDHELMMEFG 1050
ETMATFKPIL QHRPFLSKRP DQEGKEIVLN YLTPHNKWSV HSMYFDSLPM 1100
LTLFRGGPTV WMNKDDAEDT DIKDNDWIEC FNRNGVVVAR AVLSHRIPKG 1150
MAFMHHAQDR HINVPGTKLT NNRGGTHNSP TRIHVKPTQM IGGYAQLSYG 1200
FNYYGPTGNQ RDLNVVIRKL KEVDWLED 1228
Length:1,228
Mass (Da):139,099
Last modified:July 28, 2009 - v2
Checksum:i60E6B3A83422CBF8
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti47 – 471D → T in CAA90045. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49884 Genomic DNA. Translation: CAA90045.1.
X91819 Genomic DNA. Translation: CAA62926.1.
X85014 Genomic DNA. Translation: CAA59371.1.
AL009126 Genomic DNA. Translation: CAB15756.2.
PIRiS60085.
RefSeqiNP_391609.2. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAB15756; CAB15756; BSU37280.
GeneIDi938358.
KEGGibsu:BSU37280.
PATRICi18979494. VBIBacSub10457_3909.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49884 Genomic DNA. Translation: CAA90045.1 .
X91819 Genomic DNA. Translation: CAA62926.1 .
X85014 Genomic DNA. Translation: CAA59371.1 .
AL009126 Genomic DNA. Translation: CAB15756.2 .
PIRi S60085.
RefSeqi NP_391609.2. NC_000964.3.

3D structure databases

ProteinModelPortali P42175.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224308.BSU37280.

Proteomic databases

PaxDbi P42175.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai CAB15756 ; CAB15756 ; BSU37280 .
GeneIDi 938358.
KEGGi bsu:BSU37280.
PATRICi 18979494. VBIBacSub10457_3909.

Organism-specific databases

GenoListi BSU37280. [Micado ]

Phylogenomic databases

eggNOGi COG5013.
HOGENOMi HOG000237341.
KOi K00370.
OrthoDBi EOG6RC3JS.
PhylomeDBi P42175.

Enzyme and pathway databases

BioCyci BSUB:BSU37280-MONOMER.

Family and domain databases

InterProi IPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view ]
Pfami PF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view ]
SMARTi SM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view ]
SUPFAMi SSF50692. SSF50692. 1 hit.
TIGRFAMsi TIGR01580. narG. 1 hit.
PROSITEi PS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
    Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
    EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
    Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
    Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168.
  3. "The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
    Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
    FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: 168 / JH642.
  4. "The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
    Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V.
    , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
    Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 168.
  5. "From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
    Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
    Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: SEQUENCE REVISION TO 47.

Entry informationi

Entry nameiNARG_BACSU
AccessioniPrimary (citable) accession number: P42175
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 28, 2009
Last modified: July 9, 2014
This is version 113 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi