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P42175 (NARG_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Nitrate reductase alpha chain

EC=1.7.99.4
Gene names
Name:narG
Ordered Locus Names:BSU37280
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length1228 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The alpha chain is the actual site of nitrate reduction.

Catalytic activity

Nitrite + acceptor = nitrate + reduced acceptor.

Cofactor

Binds 1 4Fe-4S cluster per subunit By similarity.

Binds 1 molybdenum-bis(molybdopterin guanine dinucleotide) (Mo-bis-MGD) cofactor per subunit By similarity.

Subcellular location

Cell membrane; Peripheral membrane protein.

Sequence similarities

Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.

Contains 1 4Fe-4S Mo/W bis-MGD-type domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 12281228Nitrate reductase alpha chain
PRO_0000063232

Regions

Domain47 – 111654Fe-4S Mo/W bis-MGD-type

Sites

Metal binding541Iron-sulfur (4Fe-4S); via pros nitrogen By similarity
Metal binding581Iron-sulfur (4Fe-4S) By similarity
Metal binding621Iron-sulfur (4Fe-4S) By similarity
Metal binding971Iron-sulfur (4Fe-4S) By similarity
Metal binding2271Molybdenum By similarity

Experimental info

Sequence conflict471D → T in CAA90045. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P42175 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 60E6B3A83422CBF8

FASTA1,228139,099
        10         20         30         40         50         60 
MKKKKRSPLF RRLNYFSPIE HHSNKHSQTT REDRDWENVY RNRWQYDKVV RSTHGVNCTG 

        70         80         90        100        110        120 
SCSWNIYVKN GIVTWEGQNL NYPSTGPDMP DFEPRGCPRG ASFSWYIYSP LRVKYPYVRG 

       130        140        150        160        170        180 
VLINLWREAL QTHQNPLEAW KSIVENPEKA KSYKQARGKG GFVRAEWPEV LKLISASLLY 

       190        200        210        220        230        240 
TVMKYGPDRN VGFSPIPAMS MISHASGSRF MSLIGGPMLS FYDWYADLPP ASPQIWGDQT 

       250        260        270        280        290        300 
DVPESSDWYN SGYIITWGSN VPLTRTPDAH FLAEARYKGA KVISISPDFA ESSKFADDWL 

       310        320        330        340        350        360 
SIRQGTDGAL AMAMGHVILQ EFYVNQETER FIEYAKQYTD FPFLVTLSKE NGVYTAGRFL 

       370        380        390        400        410        420 
HAKDIGRKTK HDQWKPAVWD EQTSSFAIPQ GTMGSRWDGQ QKWNLHMIDE ETGEPIEPRL 

       430        440        450        460        470        480 
SVLGIEDEIG TVRIPYFSND GNKVLERDLP IKKMNLNGEE TYITTVFDLI LANYGVNRGI 

       490        500        510        520        530        540 
GERSAVSYDD PEPFTPAWQE QMTGIKKEAV VKIAREFAQN AIDTDGRSMI IVGAGINHWF 

       550        560        570        580        590        600 
NSDTIYRAVL NLVLLVGAQG VNGGGWAHYV GQEKLRPAEG WQTIATAKDW EGVPKLQNGT 

       610        620        630        640        650        660 
SFFYFATDQW RYEDQPISDL ASPIAASSRY KHHADYNVLA ARLGWLPSYP TFNQNGIDLY 

       670        680        690        700        710        720 
KEAEKAGAAT PEDVGAYVAS QLQEKKLKFA IEDPDNEVNF PRNLFVWRAN LISSSGKGHE 

       730        740        750        760        770        780 
YFLKHLLGTT NGLMNDDSDS IRPEEIKWRE QAPEGKLDLL INLDFRMAGT ALYSDIVLPA 

       790        800        810        820        830        840 
ATWYEKHDLS STDMHPFIHP FAPAISAPWE SKSDWDIFKA LSKAVSDLAE EVDMEPVKEV 

       850        860        870        880        890        900 
VATPLLHDTM QELAQPFGKI NDWSKGECEA IPGKTMPNIQ VVERDYKHIF HKMTALGPNV 

       910        920        930        940        950        960 
ALKPSGTKGM SWSIADEYES LKQRLGEITS DSVAKGCPNI SEAKQAAEAI LTLSSTSNGK 

       970        980        990       1000       1010       1020 
VAVKAWESLE NITNLKLKDL AEEREEECFT FEQITAQPKT VITSPAFTGS EKGGRRYSPF 

      1030       1040       1050       1060       1070       1080 
TTNVEKLIPW RTLTGRQSYY VDHELMMEFG ETMATFKPIL QHRPFLSKRP DQEGKEIVLN 

      1090       1100       1110       1120       1130       1140 
YLTPHNKWSV HSMYFDSLPM LTLFRGGPTV WMNKDDAEDT DIKDNDWIEC FNRNGVVVAR 

      1150       1160       1170       1180       1190       1200 
AVLSHRIPKG MAFMHHAQDR HINVPGTKLT NNRGGTHNSP TRIHVKPTQM IGGYAQLSYG 

      1210       1220 
FNYYGPTGNQ RDLNVVIRKL KEVDWLED 

« Hide

References

« Hide 'large scale' references
[1]"Anaerobic transcription activation in Bacillus subtilis: identification of distinct FNR-dependent and -independent regulatory mechanisms."
Cruz Ramos H., Boursier L., Moszer I., Kunst F., Danchin A., Glaser P.
EMBO J. 14:5984-5994(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees)."
Presecan E., Moszer I., Boursier L., Cruz Ramos H., De La Fuente V., Hullo M.-F., Lelong C., Schleich S., Sekowska A., Song B.H., Villani G., Kunst F., Danchin A., Glaser P.
Microbiology 143:3313-3328(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168.
[3]"The anaerobic life of Bacillus subtilis: cloning of the genes encoding the respiratory nitrate reductase system."
Hoffmann T., Troup B., Szabo A., Hungerer C., Jahn D.
FEMS Microbiol. Lett. 131:219-225(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / JH642.
[4]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[5]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 47.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49884 Genomic DNA. Translation: CAA90045.1.
X91819 Genomic DNA. Translation: CAA62926.1.
X85014 Genomic DNA. Translation: CAA59371.1.
AL009126 Genomic DNA. Translation: CAB15756.2.
PIRS60085.
RefSeqNP_391609.2. NC_000964.3.

3D structure databases

ProteinModelPortalP42175.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU37280.

Proteomic databases

PaxDbP42175.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB15756; CAB15756; BSU37280.
GeneID938358.
KEGGbsu:BSU37280.
PATRIC18979494. VBIBacSub10457_3909.

Organism-specific databases

GenoListBSU37280. [Micado]

Phylogenomic databases

eggNOGCOG5013.
HOGENOMHOG000237341.
KOK00370.
OrthoDBEOG6RC3JS.
ProtClustDBCLSK868288.

Enzyme and pathway databases

BioCycBSUB:BSU37280-MONOMER.

Family and domain databases

InterProIPR009010. Asp_de-COase-like_dom.
IPR006657. MoPterin_dinucl-bd_dom.
IPR006656. Mopterin_OxRdtase.
IPR006963. Mopterin_OxRdtase_4Fe-4S_dom.
IPR006655. Mopterin_OxRdtase_prok_CS.
IPR027467. MopterinOxRdtase_cofactor_BS.
IPR006468. NarG.
IPR028189. Nitr_red_alph_N.
[Graphical view]
PfamPF00384. Molybdopterin. 1 hit.
PF01568. Molydop_binding. 1 hit.
PF14710. Nitr_red_alph_N. 1 hit.
[Graphical view]
SMARTSM00926. Molybdop_Fe4S4. 1 hit.
[Graphical view]
SUPFAMSSF50692. SSF50692. 1 hit.
TIGRFAMsTIGR01580. narG. 1 hit.
PROSITEPS51669. 4FE4S_MOW_BIS_MGD. 1 hit.
PS00551. MOLYBDOPTERIN_PROK_1. 1 hit.
PS00490. MOLYBDOPTERIN_PROK_2. 1 hit.
PS00932. MOLYBDOPTERIN_PROK_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNARG_BACSU
AccessionPrimary (citable) accession number: P42175
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 28, 2009
Last modified: November 13, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList