Reviewed, UniProtKB/Swiss-Prot P42174 (DHE3_PIG)

Names and origin

Protein names

Recommended name:
    Glutamate dehydrogenase 1, mitochondrial
      Short name=GDH
    EC=1.4.1.3
Alternative name(s):
    Membrane protein 50
      Short name=MP50

Gene names

Name:	GLUD1
Synonyms:	GLUD

Organism

Sus scrofa (Pig)

Taxonomic identifier

9823 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Suina › Suidae › Sus

Protein attributes

Sequence length	33 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Present on the membrane of a cell organelle and confers it the capacity to interact with microtubules in a way regulated by ATP. Potential role as membrane anchor.
Catalytic activity	L-glutamate + H2O + NAD(P)+ = 2-oxoglutarate + NH3 + NAD(P)H.
Subunit structure	Homohexamer By similarity.
Subcellular location	Mitochondrion membrane; Peripheral membrane protein.
Tissue specificity	Liver, brain and thyroid.
Sequence similarities	Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	glutamate dehydrogenase [NAD(P)+] activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – ›33	›33	Glutamate dehydrogenase 1, mitochondrial		PRO_0000182741
Experimental info
	Non-adjacent residues	20 – 21	2
	Non-terminal residue	33	1

Sequences

	Sequence		Length	Mass (Da)
	P42174-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 63B6C5C3C479F4DD	FASTA	33	3,624
	10 20 30 SEAAADREDD PNFFKMVEGF GSILEADXDI LIP

References

[1]	"A membrane-bound form of glutamate dehydrogenase possesses an ATP-dependent high-affinity microtubule-binding activity." Rajas F., Rousset B. Biochem. J. 295:447-455(1993) [PubMed: 8240242] [Abstract] Cited for: PROTEIN SEQUENCE.

Cross-references

Sequence databases
PIR	S39010.
3D structure databases
ModBase	Search...
Phylogenomic databases
HOVERGEN	P42174.
Enzyme and pathway databases
BRENDA	1.4.1.3. 249.
Family and domain databases
InterPro	IPR006095. Glu/Leu/Phe/Val_DH. [Graphical view]
PROSITE	PS00074. GLFV_DEHYDROGENASE. Partial match. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DHE3_PIG

Accession

Primary (citable) accession number: P42174

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 45 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)