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Protein

Lamina-associated polypeptide 2, isoforms beta/gamma

Gene

TMPO

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May help direct the assembly of the nuclear lamina and thereby help maintain the structural organization of the nuclear envelope. Possible receptor for attachment of lamin filaments to the inner nuclear membrane. May be involved in the control of initiation of DNA replication through its interaction with NAKAP95.
Thymopoietin (TP) and Thymopentin (TP5) may play a role in T-cell development and function. TP5 is an immunomodulating pentapeptide.

GO - Molecular functioni

  • DNA binding Source: UniProtKB-KW
  • lamin binding Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120802-MONOMER.
ReactomeiR-HSA-2993913. Clearance of Nuclear Envelope Membranes from Chromatin.
R-HSA-2995383. Initiation of Nuclear Envelope Reformation.
R-HSA-4419969. Depolymerisation of the Nuclear Lamina.

Names & Taxonomyi

Protein namesi
Recommended name:
Lamina-associated polypeptide 2, isoforms beta/gamma
Alternative name(s):
Thymopoietin, isoforms beta/gamma
Short name:
TP beta/gamma
Thymopoietin-related peptide isoforms beta/gamma
Short name:
TPRP isoforms beta/gamma
Cleaved into the following 2 chains:
Thymopoietin
Short name:
TP
Alternative name(s):
Splenin
Alternative name(s):
TP5
Gene namesi
Name:TMPO
Synonyms:LAP2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 12

Organism-specific databases

HGNCiHGNC:11875. TMPO.

Subcellular locationi

Isoform Zeta :

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei411 – 434Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST24
Topological domaini435 – 454LumenalSequence analysisAdd BLAST20

GO - Cellular componenti

  • cytoplasm Source: UniProtKB-SubCell
  • integral component of membrane Source: UniProtKB-KW
  • membrane Source: UniProtKB
  • nuclear envelope Source: BHF-UCL
  • nuclear inner membrane Source: UniProtKB-SubCell
  • nuclear membrane Source: HPA
  • nucleus Source: ProtInc
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Pharmaceutical usei

TP5 is available under the names Timunox (Cilag), Sintomodulina (Italofarmaco) and Mepentil (Recordati). Used in primary and secondary immune deficiencies, autoimmunity, infections and cancer.

Organism-specific databases

DisGeNETi7112.
MalaCardsiTMPO.
OpenTargetsiENSG00000120802.
PharmGKBiPA36576.

Polymorphism and mutation databases

BioMutaiPLCE1.
DMDMi1174690.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000458411 – 454Lamina-associated polypeptide 2, isoforms beta/gammaAdd BLAST454
PeptideiPRO_00000176771 – 50ThymopoietinAdd BLAST50
PeptideiPRO_000001767833 – 37Thymopentin5

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei57PhosphothreonineCombined sources1
Modified residuei59PhosphoserineCombined sources1
Modified residuei66PhosphoserineCombined sources1
Modified residuei67PhosphoserineCombined sources1
Modified residuei74PhosphothreonineCombined sources1
Modified residuei79PhosphoserineCombined sources1
Modified residuei86Omega-N-methylarginineBy similarity1
Modified residuei88Omega-N-methylarginineBy similarity1
Modified residuei154PhosphothreonineCombined sources1
Modified residuei156PhosphoserineCombined sources1
Modified residuei159PhosphoserineBy similarity1
Modified residuei160PhosphothreonineCombined sources1
Modified residuei164PhosphothreonineCombined sources1
Modified residuei166PhosphoserineBy similarity1
Modified residuei168PhosphoserineCombined sources1
Modified residuei177PhosphoserineCombined sources1
Modified residuei180PhosphoserineCombined sources1
Modified residuei184PhosphoserineCombined sources1
Modified residuei190PhosphoserineCombined sources1
Modified residuei207N6-acetyllysineBy similarity1
Modified residuei211PhosphothreonineCombined sources1
Modified residuei222PhosphoserineCombined sources1
Modified residuei224PhosphoserineCombined sources1
Modified residuei250PhosphoserineCombined sources1
Modified residuei254PhosphoserineCombined sources1
Modified residuei265PhosphoserineCombined sources1
Modified residuei292PhosphoserineCombined sources1
Modified residuei306PhosphoserineCombined sources1
Modified residuei312PhosphothreonineCombined sources1
Modified residuei315PhosphoserineCombined sources1
Modified residuei320CitrullineBy similarity1
Modified residuei362PhosphoserineCombined sources1
Modified residuei378PhosphoserineCombined sources1
Modified residuei385PhosphoserineCombined sources1
Modified residuei389N6-acetyllysineBy similarity1
Modified residuei402PhosphoserineCombined sources1

Post-translational modificationi

Mitosis-specific phosphorylation specifically abolishes its binding to lamin B and chromosomes.By similarity
Citrullinated by PADI4.By similarity

Keywords - PTMi

Acetylation, Citrullination, Methylation, Phosphoprotein

Proteomic databases

EPDiP42167.
MaxQBiP42167.
PaxDbiP42167.
PeptideAtlasiP42167.
PRIDEiP42167.
TopDownProteomicsiP42167-1. [P42167-1]
P42167-2. [P42167-2]

PTM databases

iPTMnetiP42167.
PhosphoSitePlusiP42167.
SwissPalmiP42167.

Miscellaneous databases

PMAP-CutDBP42167.

Expressioni

Tissue specificityi

Expressed in many tissues. Most abundant in adult thymus and fetal liver.

Gene expression databases

BgeeiENSG00000120802.
CleanExiHS_TMPO.
ExpressionAtlasiP42167. baseline and differential.
GenevisibleiP42167. HS.

Organism-specific databases

HPAiCAB009847.
HPA008150.

Interactioni

Subunit structurei

Interacts with LMNB1, LMNB2, BANF1, AKAP8L, GMCL and chromosomes (By similarity). Isoform Zeta interacts with BANF1/BAF and may sequester it in the cytoplasm.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
NCK1P163332EBI-455283,EBI-389883

GO - Molecular functioni

  • lamin binding Source: ProtInc

Protein-protein interaction databases

BioGridi112967. 105 interactors.
DIPiDIP-43686N.
IntActiP42167. 46 interactors.
MINTiMINT-2839042.
STRINGi9606.ENSP00000266732.

Structurei

3D structure databases

ProteinModelPortaliP42167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 48LEM-likePROSITE-ProRule annotationAdd BLAST44
Domaini109 – 153LEMPROSITE-ProRule annotationAdd BLAST45

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 410NucleoplasmicSequence analysisAdd BLAST410
Regioni49 – 108LinkerAdd BLAST60
Regioni138 – 243NAKAP95-binding NAdd BLAST106
Regioni299 – 371Binds lamins BAdd BLAST73
Regioni300 – 374NAKAP95-binding CAdd BLAST75

Domaini

Has two structurally independent, non-interacting domains: LEM-like (also called LAP2-N or LEM-D) and LEM (also called LAP2-C or LEM-B). LEM-like binds DNA while LEM interacts with BANF1.

Sequence similaritiesi

Belongs to the LEM family.Curated
Contains 1 LEM domain.PROSITE-ProRule annotation
Contains 1 LEM-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IT7C. Eukaryota.
ENOG4111J0N. LUCA.
GeneTreeiENSGT00510000048934.
HOGENOMiHOG000113280.
HOVERGENiHBG000166.
OMAiGNFKHAA.

Family and domain databases

Gene3Di1.10.720.40. 2 hits.
InterProiIPR013146. LEM-like_dom.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
SM01261. Thymopoietin. 1 hit.
[Graphical view]
SUPFAMiSSF63451. SSF63451. 2 hits.
PROSITEiPS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.
[Graphical view]

Sequences (4)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.
Isoform Beta (identifier: P42167-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPEFLEDPSV LTKDKLKSEL VANNVTLPAG EQRKDVYVQL YLQHLTARNR
60 70 80 90 100
PPLPAGTNSK GPPDFSSDEE REPTPVLGSG AAAAGRSRAA VGRKATKKTD
110 120 130 140 150
KPRQEDKDDL DVTELTNEDL LDQLVKYGVN PGPIVGTTRK LYEKKLLKLR
160 170 180 190 200
EQGTESRSST PLPTISSSAE NTRQNGSNDS DRYSDNEEDS KIELKLEKRE
210 220 230 240 250
PLKGRAKTPV TLKQRRVEHN QSYSQAGITE TEWTSGSSKG GPLQALTRES
260 270 280 290 300
TRGSRRTPRK RVETSEHFRI DGPVISESTP IAETIMASSN ESLVVNRVTG
310 320 330 340 350
NFKHASPILP ITEFSDIPRR APKKPLTRAE VGEKTEERRV ERDILKEMFP
360 370 380 390 400
YEASTPTGIS ASCRRPIKGA AGRPLELSDF RMEESFSSKY VPKYVPLADV
410 420 430 440 450
KSEKTKKGRS IPVWIKILLF VVVAVFLFLV YQAMETNQVN PFSNFLHVDP

RKSN
Length:454
Mass (Da):50,670
Last modified:January 23, 2007 - v2
Checksum:i03277C5723117909
GO
Isoform Alpha (identifier: P42166-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry P42166.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:694
Mass (Da):75,492
GO
Isoform Gamma (identifier: P42167-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-330: Missing.

Show »
Length:345
Mass (Da):38,738
Checksum:iF0669D9AEF97B32E
GO
Isoform Zeta (identifier: P42167-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     222-248: SYSQAGITETEWTSGSSKGGPLQALTR → VSLVLLPPCTGINNLLTTLIHVLAFNG
     249-454: Missing.

Note: Inhibits LAP2beta-mediated repression.
Show »
Length:248
Mass (Da):27,388
Checksum:iC3CDE62D4C0B0590
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_049779287A → P.Corresponds to variant rs7133258dbSNPEnsembl.1
Natural variantiVAR_014786427L → F.Corresponds to variant rs1058288dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_004456222 – 330Missing in isoform Gamma. 2 PublicationsAdd BLAST109
Alternative sequenceiVSP_056162222 – 248SYSQA…QALTR → VSLVLLPPCTGINNLLTTLI HVLAFNG in isoform Zeta. 1 PublicationAdd BLAST27
Alternative sequenceiVSP_056163249 – 454Missing in isoform Zeta. 1 PublicationAdd BLAST206

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09087 mRNA. Translation: AAB60330.1.
U09088 mRNA. Translation: AAB60331.1.
EF028063 mRNA. Translation: ABL61272.1.
AF070631 mRNA. Translation: AAC25390.1.
BC053675 mRNA. Translation: AAH53675.1.
U18269
, U18266, U18267, U18268, U18270 Genomic DNA. Translation: AAB60434.1.
U18271 Genomic DNA. Translation: AAB60435.1.
CCDSiCCDS31879.1. [P42167-1]
CCDS31880.1. [P42167-2]
PIRiB55741.
C55741.
RefSeqiNP_001027454.1. NM_001032283.2. [P42167-1]
NP_001027455.1. NM_001032284.2. [P42167-2]
NP_001294904.1. NM_001307975.1.
UniGeneiHs.11355.

Genome annotation databases

EnsembliENST00000261210; ENSP00000261210; ENSG00000120802. [P42167-3]
ENST00000393053; ENSP00000376773; ENSG00000120802. [P42167-2]
ENST00000556029; ENSP00000450627; ENSG00000120802. [P42167-1]
GeneIDi7112.
KEGGihsa:7112.
UCSCiuc001tfi.3. human. [P42167-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09087 mRNA. Translation: AAB60330.1.
U09088 mRNA. Translation: AAB60331.1.
EF028063 mRNA. Translation: ABL61272.1.
AF070631 mRNA. Translation: AAC25390.1.
BC053675 mRNA. Translation: AAH53675.1.
U18269
, U18266, U18267, U18268, U18270 Genomic DNA. Translation: AAB60434.1.
U18271 Genomic DNA. Translation: AAB60435.1.
CCDSiCCDS31879.1. [P42167-1]
CCDS31880.1. [P42167-2]
PIRiB55741.
C55741.
RefSeqiNP_001027454.1. NM_001032283.2. [P42167-1]
NP_001027455.1. NM_001032284.2. [P42167-2]
NP_001294904.1. NM_001307975.1.
UniGeneiHs.11355.

3D structure databases

ProteinModelPortaliP42167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi112967. 105 interactors.
DIPiDIP-43686N.
IntActiP42167. 46 interactors.
MINTiMINT-2839042.
STRINGi9606.ENSP00000266732.

PTM databases

iPTMnetiP42167.
PhosphoSitePlusiP42167.
SwissPalmiP42167.

Polymorphism and mutation databases

BioMutaiPLCE1.
DMDMi1174690.

Proteomic databases

EPDiP42167.
MaxQBiP42167.
PaxDbiP42167.
PeptideAtlasiP42167.
PRIDEiP42167.
TopDownProteomicsiP42167-1. [P42167-1]
P42167-2. [P42167-2]

Protocols and materials databases

DNASUi7112.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000261210; ENSP00000261210; ENSG00000120802. [P42167-3]
ENST00000393053; ENSP00000376773; ENSG00000120802. [P42167-2]
ENST00000556029; ENSP00000450627; ENSG00000120802. [P42167-1]
GeneIDi7112.
KEGGihsa:7112.
UCSCiuc001tfi.3. human. [P42167-1]

Organism-specific databases

CTDi7112.
DisGeNETi7112.
GeneCardsiTMPO.
GeneReviewsiTMPO.
HGNCiHGNC:11875. TMPO.
HPAiCAB009847.
HPA008150.
MalaCardsiTMPO.
MIMi188380. gene.
neXtProtiNX_P42167.
OpenTargetsiENSG00000120802.
PharmGKBiPA36576.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IT7C. Eukaryota.
ENOG4111J0N. LUCA.
GeneTreeiENSGT00510000048934.
HOGENOMiHOG000113280.
HOVERGENiHBG000166.
OMAiGNFKHAA.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000120802-MONOMER.
ReactomeiR-HSA-2993913. Clearance of Nuclear Envelope Membranes from Chromatin.
R-HSA-2995383. Initiation of Nuclear Envelope Reformation.
R-HSA-4419969. Depolymerisation of the Nuclear Lamina.

Miscellaneous databases

ChiTaRSiTMPO. human.
GenomeRNAii7112.
PMAP-CutDBP42167.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000120802.
CleanExiHS_TMPO.
ExpressionAtlasiP42167. baseline and differential.
GenevisibleiP42167. HS.

Family and domain databases

Gene3Di1.10.720.40. 2 hits.
InterProiIPR013146. LEM-like_dom.
IPR011015. LEM/LEM-like_dom.
IPR003887. LEM_dom.
[Graphical view]
PfamiPF03020. LEM. 1 hit.
PF08198. Thymopoietin. 1 hit.
[Graphical view]
SMARTiSM00540. LEM. 1 hit.
SM01261. Thymopoietin. 1 hit.
[Graphical view]
SUPFAMiSSF63451. SSF63451. 2 hits.
PROSITEiPS50954. LEM. 1 hit.
PS50955. LEM_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLAP2B_HUMAN
AccessioniPrimary (citable) accession number: P42167
Secondary accession number(s): A2T926, Q14861
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 173 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Pharmaceutical, Reference proteome

Documents

  1. Human chromosome 12
    Human chromosome 12: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.