Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P42128

- FOXK1_MOUSE

UniProt

P42128 - FOXK1_MOUSE

Protein

Forkhead box protein K1

Gene

Foxk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 2 (28 Nov 2006)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcriptional regulator that binds to the upstream enhancer region (CCAC box) of myoglobin gene. Has a role in myogenic differentiation and in remodeling processes of adult muscles that occur in response to physiological stimuli.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi291 – 38696Fork-headPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. protein binding Source: IntAct
    3. RNA polymerase II regulatory region sequence-specific DNA binding Source: NTNU_SB
    4. sequence-specific DNA binding Source: UniProtKB
    5. sequence-specific DNA binding RNA polymerase II transcription factor activity Source: NTNU_SB

    GO - Biological processi

    1. cell differentiation Source: UniProtKB-KW
    2. muscle organ development Source: UniProtKB-KW
    3. negative regulation of cell cycle Source: UniProtKB
    4. negative regulation of cell growth Source: UniProtKB
    5. negative regulation of transcription, DNA-templated Source: UniProtKB
    6. positive regulation of transcription, DNA-templated Source: UniProtKB
    7. regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
    8. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Activator, Developmental protein, Repressor

    Keywords - Biological processi

    Differentiation, Myogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Forkhead box protein K1
    Alternative name(s):
    Myocyte nuclear factor
    Short name:
    MNF
    Gene namesi
    Name:Foxk1
    Synonyms:Mnf
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1347488. Foxk1.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleolus Source: Ensembl
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 719718Forkhead box protein K1PRO_0000091857Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylalanineBy similarity
    Modified residuei87 – 871PhosphoserineBy similarity
    Modified residuei199 – 1991PhosphoserineBy similarity
    Modified residuei209 – 2091PhosphoserineBy similarity
    Modified residuei225 – 2251PhosphoserineBy similarity
    Modified residuei229 – 2291PhosphoserineBy similarity
    Modified residuei239 – 2391PhosphoserineBy similarity
    Modified residuei243 – 2431PhosphoserineBy similarity
    Modified residuei285 – 2851PhosphoserineBy similarity
    Modified residuei402 – 4021PhosphoserineBy similarity
    Modified residuei414 – 4141PhosphoserineBy similarity
    Modified residuei422 – 4221Phosphothreonine2 Publications
    Modified residuei427 – 4271PhosphoserineBy similarity
    Modified residuei431 – 4311PhosphoserineBy similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP42128.
    PaxDbiP42128.
    PRIDEiP42128.

    PTM databases

    PhosphoSiteiP42128.

    Expressioni

    Tissue specificityi

    Expressed in tissues and cells in which the myoglobin gene is transcriptionally active including cardiac and skeletal myocytes, brain and kidney. In the adult brain, expressed in the piriform cortex and the indusium griseum. In the hippocampus, expression is localized to the dentate gyrus and CA3 area. In the cerebellum, expression is confined to the Purkinje cell layer.3 Publications

    Gene expression databases

    BgeeiP42128.
    CleanExiMM_FOXK1.
    GenevestigatoriP42128.

    Interactioni

    Subunit structurei

    Interacts with SIN3B to form a complex which represses transcription.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Sin3bQ62141-211EBI-878270,EBI-591466

    Protein-protein interaction databases

    IntActiP42128. 2 interactions.
    MINTiMINT-4095257.

    Structurei

    Secondary structure

    1
    719
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi292 – 2943
    Helixi298 – 3047
    Beta strandi307 – 3126
    Helixi314 – 32411
    Beta strandi326 – 3305
    Helixi335 – 34511
    Beta strandi347 – 35610
    Beta strandi360 – 3623
    Beta strandi365 – 3673
    Helixi372 – 3809

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2A3SNMR-A289-389[»]
    2D2WNMR-A289-389[»]
    ProteinModelPortaliP42128.
    SMRiP42128. Positions 289-389.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP42128.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini109 – 16153FHAPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi10 – 8273Ala-richAdd
    BLAST
    Compositional biasi35 – 6329Pro-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 FHA domain.PROSITE-ProRule annotation
    Contains 1 fork-head DNA-binding domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5025.
    GeneTreeiENSGT00750000117242.
    HOGENOMiHOG000072588.
    HOVERGENiHBG051649.
    InParanoidiP42128.
    KOiK09404.
    OMAiCEVGPKE.
    OrthoDBiEOG7CRTS2.
    PhylomeDBiP42128.
    TreeFamiTF325718.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    2.60.200.20. 1 hit.
    InterProiIPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PfamiPF00498. FHA. 1 hit.
    PF00250. Fork_head. 1 hit.
    [Graphical view]
    PRINTSiPR00053. FORKHEAD.
    SMARTiSM00339. FH. 1 hit.
    SM00240. FHA. 1 hit.
    [Graphical view]
    SUPFAMiSSF49879. SSF49879. 1 hit.
    PROSITEiPS50006. FHA_DOMAIN. 1 hit.
    PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P42128-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAEVGEDSGA RALLALRSAP CSPVLCAAAA AAAFPATTSP PPPAQPPPGP    50
    PALPAEPGPG PVPSTVATAT TTAPALVAAA AASVRQSPGP ALARLEGREF 100
    EFLMRQPSVT IGRNSSQGSV DLSMGLSSFI SRRHLQLSFQ EPHFYLRCLG 150
    KNGVFVDGAF QRRGAPALQL PQQCTFRFPS TAIKIQFTSL YHKEEAPASP 200
    LRPLYPQISP LKIHIPEPDL RSLVSPIPSP TGTISVPNSC PASPRGAGSS 250
    SYRFVQNVTS DLQLAAEFAA KAASEQQADA SGGDSPKDES KPPYSYAQLI 300
    VQAISSAQDR QLTLSGIYAH ITKHYPYYRT ADKGWQNSIR HNLSLNRYFI 350
    KVPRSQEEPG KGSFWRIDPA SEAKLVEQAF RKRRQRGVSC FRTPFGPLSS 400
    RSAPASPTHP GLMSPRSSGL QTPECLSREG SPIPHDPDLG SKLASVPEYR 450
    YSQSAPGSPV SAQPVIMAVP PRPSNLVAKP VAYMPASIVT SQQPSGHAIH 500
    VVQQAPTVTM VRVVTTSANS ANGYILASQG STGTSHDTAG TAVLDLGNEA 550
    RGLEEKPTIA FATIPAASRV IQTVASQMAP GVPGHTVTIL QPATPVTIGQ 600
    HHLPVRAVTQ NGKHAVPTNS LTGNAYALSS PLQLLAAQAS SSTPVVITRV 650
    CEVGPEEPAA AVSVAANAAP TPAASTTTSA SSSGEPEVKR SRVEEPGGTA 700
    TTQPTAMAAT GPQGPGTGE 719
    Length:719
    Mass (Da):74,920
    Last modified:November 28, 2006 - v2
    Checksum:i0A0D3049B7846328
    GO

    Sequence cautioni

    The sequence AAA37529.1 differs from that shown. Reason: Frameshift at positions 61, 88, 582, 603, 615 and 711.
    The sequence AAB69641.1 differs from that shown. Reason: Frameshift at positions 61 and 88.
    The sequence BAE25880.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti43 – 431Missing in AAA37529. (PubMed:8007964)Curated
    Sequence conflicti43 – 431Missing in AAB69641. (PubMed:9271401)Curated
    Sequence conflicti402 – 4032SA → RS in AAA37529. (PubMed:8007964)Curated
    Sequence conflicti407 – 4115PTHPG → HTSHA in AAB69641. (PubMed:9271401)Curated
    Sequence conflicti551 – 5511R → A in AAA37529. (PubMed:8007964)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L26507 mRNA. Translation: AAA37529.1. Frameshift.
    AK144418 mRNA. Translation: BAE25880.1. Different initiation.
    AK147375 mRNA. Translation: BAE27871.1.
    U95016 mRNA. Translation: AAB69641.1. Frameshift.
    CCDSiCCDS19826.1.
    PIRiA56051.
    RefSeqiNP_951031.2. NM_199068.2.
    UniGeneiMm.24214.

    Genome annotation databases

    EnsembliENSMUST00000072837; ENSMUSP00000072616; ENSMUSG00000056493.
    GeneIDi17425.
    KEGGimmu:17425.
    UCSCiuc009aio.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L26507 mRNA. Translation: AAA37529.1 . Frameshift.
    AK144418 mRNA. Translation: BAE25880.1 . Different initiation.
    AK147375 mRNA. Translation: BAE27871.1 .
    U95016 mRNA. Translation: AAB69641.1 . Frameshift.
    CCDSi CCDS19826.1.
    PIRi A56051.
    RefSeqi NP_951031.2. NM_199068.2.
    UniGenei Mm.24214.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2A3S NMR - A 289-389 [» ]
    2D2W NMR - A 289-389 [» ]
    ProteinModelPortali P42128.
    SMRi P42128. Positions 289-389.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P42128. 2 interactions.
    MINTi MINT-4095257.

    PTM databases

    PhosphoSitei P42128.

    Proteomic databases

    MaxQBi P42128.
    PaxDbi P42128.
    PRIDEi P42128.

    Protocols and materials databases

    DNASUi 17425.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000072837 ; ENSMUSP00000072616 ; ENSMUSG00000056493 .
    GeneIDi 17425.
    KEGGi mmu:17425.
    UCSCi uc009aio.1. mouse.

    Organism-specific databases

    CTDi 221937.
    MGIi MGI:1347488. Foxk1.

    Phylogenomic databases

    eggNOGi COG5025.
    GeneTreei ENSGT00750000117242.
    HOGENOMi HOG000072588.
    HOVERGENi HBG051649.
    InParanoidi P42128.
    KOi K09404.
    OMAi CEVGPKE.
    OrthoDBi EOG7CRTS2.
    PhylomeDBi P42128.
    TreeFami TF325718.

    Miscellaneous databases

    ChiTaRSi FOXK1. mouse.
    EvolutionaryTracei P42128.
    NextBioi 292048.
    PROi P42128.
    SOURCEi Search...

    Gene expression databases

    Bgeei P42128.
    CleanExi MM_FOXK1.
    Genevestigatori P42128.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    2.60.200.20. 1 hit.
    InterProi IPR000253. FHA_dom.
    IPR008984. SMAD_FHA_domain.
    IPR001766. TF_fork_head.
    IPR018122. TF_fork_head_CS.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    Pfami PF00498. FHA. 1 hit.
    PF00250. Fork_head. 1 hit.
    [Graphical view ]
    PRINTSi PR00053. FORKHEAD.
    SMARTi SM00339. FH. 1 hit.
    SM00240. FHA. 1 hit.
    [Graphical view ]
    SUPFAMi SSF49879. SSF49879. 1 hit.
    PROSITEi PS50006. FHA_DOMAIN. 1 hit.
    PS00657. FORK_HEAD_1. 1 hit.
    PS00658. FORK_HEAD_2. 1 hit.
    PS50039. FORK_HEAD_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Myocyte nuclear factor, a novel winged-helix transcription factor under both developmental and neural regulation in striated myocytes."
      Bassel-Duby R., Hernandez M.D., Yang Q., Rochelle J.M., Seldin M.F., Williams R.S.
      Mol. Cell. Biol. 14:4596-4605(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, PHOSPHORYLATION.
      Tissue: Pancreatic acinar cell.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Diencephalon.
    3. "Transient expression of a winged-helix protein, MNF-beta, during myogenesis."
      Yang Q., Bassel-Duby R., Williams R.S.
      Mol. Cell. Biol. 17:5236-5243(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-411, FUNCTION, TISSUE SPECIFICITY.
      Tissue: Skeletal muscle.
    4. "The winged-helix/forkhead protein myocyte nuclear factor beta (MNF-beta) forms a co-repressor complex with mammalian Sin3B."
      Yang Q., Kong Y., Rothermel B., Garry D.J., Bassel-Duby R., Williams R.S.
      Biochem. J. 345:335-343(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH SIN3B.
      Tissue: Heart.
    5. "Identification of forkhead transcription factors in cortical and dopaminergic areas of the adult murine brain."
      Wijchers P.J.E.C., Hoekman M.F.M., Burbach J.P.H., Smidt M.P.
      Brain Res. 1068:23-33(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-422, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.

    Entry informationi

    Entry nameiFOXK1_MOUSE
    AccessioniPrimary (citable) accession number: P42128
    Secondary accession number(s): O35939, Q3UHI6, Q3UN67
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 28, 2006
    Last modified: October 1, 2014
    This is version 145 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3