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Protein

Histone-lysine N-methyltransferase E(z)

Gene

E(z)

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Polycomb group (PcG) protein. Catalytic subunit of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene. While PcG proteins are generally required to maintain the transcriptionally repressive state of homeotic genes throughout development, this protein is specifically required during the first 6 hours of embryogenesis to establish the repressed state. The Esc/E(z) complex is necessary but not sufficient for the repression of homeotic target genes, suggesting that the recruitment of the distinct PRC1 complex is also required.

Catalytic activityi

S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone].

GO - Molecular functioni

  • DNA binding Source: UniProtKB
  • DNA binding transcription factor activity Source: UniProtKB
  • histone methyltransferase activity Source: FlyBase
  • histone methyltransferase activity (H3-K27 specific) Source: FlyBase
  • histone methyltransferase activity (H3-K9 specific) Source: FlyBase

GO - Biological processi

  • axon guidance Source: FlyBase
  • cuticle hydrocarbon biosynthetic process Source: FlyBase
  • gene silencing Source: UniProtKB
  • histone H3-K27 methylation Source: FlyBase
  • histone H3-K9 methylation Source: FlyBase
  • histone methylation Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: UniProtKB
  • neurogenesis Source: FlyBase
  • positive regulation of histone methylation Source: UniProtKB
  • regulation of neuroblast proliferation Source: UniProtKB
  • syncytial blastoderm mitotic cell cycle Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionChromatin regulator, Developmental protein, Methyltransferase, Repressor, Transferase
Biological processTranscription, Transcription regulation
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

ReactomeiR-DME-212300 PRC2 methylates histones and DNA
R-DME-2559580 Oxidative Stress Induced Senescence
R-DME-3214841 PKMTs methylate histone lysines
R-DME-8943724 Regulation of PTEN gene transcription
R-DME-8953750 Transcriptional Regulation by E2F6
SignaLinkiP42124

Names & Taxonomyi

Protein namesi
Recommended name:
Histone-lysine N-methyltransferase E(z) (EC:2.1.1.43)
Alternative name(s):
Lysine N-methyltransferase 6
Protein enhancer of zeste
Gene namesi
Name:E(z)
Synonyms:KMT6
ORF Names:CG6502
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3L

Organism-specific databases

FlyBaseiFBgn0000629 E(z)

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi699R → A or H: Strongly reduces methyltransferase activity of the Esc/E(z) complex. 1 Publication1
Mutagenesisi702 – 703NH → AA: Abolishes methyltransferase activity of the Esc/E(z) complex. 1 Publication2
Mutagenesisi703H → A or K: Strongly reduces methyltransferase activity of the Esc/E(z) complex. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2169719

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002139891 – 760Histone-lysine N-methyltransferase E(z)Add BLAST760

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei493Phosphoserine1 Publication1
Modified residuei502Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP42124
PRIDEiP42124

PTM databases

iPTMnetiP42124

Expressioni

Gene expression databases

BgeeiFBgn0000629
ExpressionAtlasiP42124 differential
GenevisibleiP42124 DM

Interactioni

Subunit structurei

Component of the Esc/E(z) complex, composed of Caf1, esc, E(z), Su(z)12, and possibly pho. The Esc/E(z) complex may also associate with Pcl and Rpd3 during early embryogenesis. This complex is distinct from the PRC1 complex, which contains many other PcG proteins like Pc, Ph, Psc, Su(z)2. The two complexes however cooperate and interact together during the first 3 hours of development to establish PcG silencing. Interacts with corto in vitro.8 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi6458232 interactors.
DIPiDIP-20386N
IntActiP42124 24 interactors.
MINTiP42124
STRINGi7227.FBpp0306192

Chemistry databases

BindingDBiP42124

Structurei

3D structure databases

ProteinModelPortaliP42124
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini443 – 491SANTAdd BLAST49
Domaini518 – 619CXCPROSITE-ProRule annotationAdd BLAST102
Domaini626 – 741SETPROSITE-ProRule annotationAdd BLAST116

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi505 – 510Nuclear localization signalSequence analysis6

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi538 – 619Cys-richAdd BLAST82

Sequence similaritiesi

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. EZ subfamily.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG1079 Eukaryota
COG2940 LUCA
GeneTreeiENSGT00780000121845
InParanoidiP42124
KOiK11430
OrthoDBiEOG091G09L0
PhylomeDBiP42124

Family and domain databases

CDDicd00167 SANT, 1 hit
InterProiView protein in InterPro
IPR026489 CXC_dom
IPR001005 SANT/Myb
IPR001214 SET_dom
IPR033467 Tesmin/TSO1-like_CXC
PfamiView protein in Pfam
PF00856 SET, 1 hit
SMARTiView protein in SMART
SM01114 CXC, 1 hit
SM00717 SANT, 2 hits
SM00317 SET, 1 hit
PROSITEiView protein in PROSITE
PS51633 CXC, 1 hit
PS50280 SET, 1 hit

Sequencei

Sequence statusi: Complete.

P42124-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNSTKVPPEW KRRVKSEYIK IRQQKRYKRA DEIKEAWIRN WDEHNHNVQD
60 70 80 90 100
LYCESKVWQA KPYDPPHVDC VKRAEVTSYN GIPSGPQKVP ICVINAVTPI
110 120 130 140 150
PTMYTWAPTQ QNFMVEDETV LHNIPYMGDE VLDKDGKFIE ELIKNYDGKV
160 170 180 190 200
HGDKDPSFMD DAIFVELVHA LMRSYSKELE EAAPGTATAI KTETLAKSKQ
210 220 230 240 250
GEDDGVVDVD ADGESPMKLE KTDSKGDLTE VEKKETEEPL ETEDADVKPD
260 270 280 290 300
VEEVKDKLPF PAPIIFQAIS ANFPDKGTAQ ELKEKYIELT EHQDPERPQE
310 320 330 340 350
CTPNIDGIKA ESVSRERTMH SFHTLFCRRC FKYDCFLHRL QGHAGPNLQK
360 370 380 390 400
RRYPELKPFA EPCSNSCYML IDGMKEKLAA DSKTPPIDSC NEASSEDSND
410 420 430 440 450
SNSQFSNKDF NHENSKDNGL TVNSAAVAEI NSIMAGMMNI TSTQCVWTGA
460 470 480 490 500
DQALYRVLHK VYLKNYCAIA HNMLTKTCRQ VYEFAQKEDA EFSFEDLRQD
510 520 530 540 550
FTPPRKKKKK QRLWSLHCRK IQLKKDSSSN HVYNYTPCDH PGHPCDMNCS
560 570 580 590 600
CIQTQNFCEK FCNCSSDCQN RFPGCRCKAQ CNTKQCPCYL AVRECDPDLC
610 620 630 640 650
QACGADQFKL TKITCKNVCV QRGLHKHLLM APSDIAGWGI FLKEGAQKNE
660 670 680 690 700
FISEYCGEII SQDEADRRGK VYDKYMCSFL FNLNNDFVVD ATRKGNKIRF
710 720 730 740 750
ANHSINPNCY AKVMMVTGDH RIGIFAKRAI QPGEELFFDY RYGPTEQLKF
760
VGIEREMEIV
Length:760
Mass (Da):86,935
Last modified:March 28, 2003 - v2
Checksum:i022360ED7772EB60
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185 – 187GTA → STS in AAC46462 (PubMed:8413234).Curated3
Sequence conflicti194T → P in AAC46462 (PubMed:8413234).Curated1
Sequence conflicti213G → C in AAC46462 (PubMed:8413234).Curated1
Sequence conflicti223D → E in AAC46462 (PubMed:8413234).Curated1
Sequence conflicti230E → D in AAC46462 (PubMed:8413234).Curated1
Sequence conflicti240L → V in AAC46462 (PubMed:8413234).Curated1
Sequence conflicti250D → A in AAC46462 (PubMed:8413234).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00180 mRNA Translation: AAC46462.1
AE014296 Genomic DNA Translation: AAF50149.1
AE014296 Genomic DNA Translation: ACL83287.1
AY051785 mRNA Translation: AAK93209.1
RefSeqiNP_001137932.1, NM_001144460.3
NP_524021.2, NM_079297.3
UniGeneiDm.2823

Genome annotation databases

EnsemblMetazoaiFBtr0076279; FBpp0076008; FBgn0000629
FBtr0273338; FBpp0271846; FBgn0000629
GeneIDi39203
KEGGidme:Dmel_CG6502

Similar proteinsi

Entry informationi

Entry nameiEZ_DROME
AccessioniPrimary (citable) accession number: P42124
Secondary accession number(s): B7Z0G2, Q9VTA3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: March 28, 2003
Last modified: March 28, 2018
This is version 171 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome