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Protein

L-lactate dehydrogenase B chain

Gene

Ldhb

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-lactate + NAD+ = pyruvate + NADH.

Pathwayi: pyruvate fermentation to lactate

This protein is involved in step 1 of the subpathway that synthesizes (S)-lactate from pyruvate.
Proteins known to be involved in this subpathway in this organism are:
  1. L-lactate dehydrogenase A chain (Ldha), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhal6b), L-lactate dehydrogenase B chain (Ldhb), L-lactate dehydrogenase C chain (Ldhc), L-lactate dehydrogenase (Ldha), L-lactate dehydrogenase (Ldhc)
This subpathway is part of the pathway pyruvate fermentation to lactate, which is itself part of Fermentation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-lactate from pyruvate, the pathway pyruvate fermentation to lactate and in Fermentation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei107SubstrateBy similarity1
Binding sitei139NAD or substrateBy similarity1
Binding sitei170SubstrateBy similarity1
Active sitei194Proton acceptorBy similarity1
Binding sitei249SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 58NADBy similarityAdd BLAST29

GO - Molecular functioni

  • identical protein binding Source: RGD
  • kinase binding Source: RGD
  • lactate dehydrogenase activity Source: RGD
  • L-lactate dehydrogenase activity Source: RGD
  • NAD binding Source: RGD

GO - Biological processi

  • carbohydrate metabolic process Source: InterPro
  • lactate metabolic process Source: RGD
  • NAD metabolic process Source: RGD

Keywordsi

Molecular functionOxidoreductase
LigandNAD

Enzyme and pathway databases

ReactomeiR-RNO-70268. Pyruvate metabolism.
UniPathwayiUPA00554; UER00611.

Names & Taxonomyi

Protein namesi
Recommended name:
L-lactate dehydrogenase B chain (EC:1.1.1.27)
Short name:
LDH-B
Alternative name(s):
LDH heart subunit
Short name:
LDH-H
Gene namesi
Name:Ldhb
Synonyms:Ldh-2, Ldh2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi2997. Ldhb.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2176834.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001684642 – 334L-lactate dehydrogenase B chainAdd BLAST333

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei7N6-acetyllysineBy similarity1
Modified residuei44PhosphoserineCombined sources1
Modified residuei58N6-acetyllysineBy similarity1
Modified residuei119N6-acetyllysineBy similarity1
Modified residuei240PhosphotyrosineBy similarity1
Modified residuei329N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiP42123.
PRIDEiP42123.

2D gel databases

World-2DPAGEi0004:P42123.

PTM databases

iPTMnetiP42123.
PhosphoSitePlusiP42123.
SwissPalmiP42123.

Expressioni

Gene expression databases

BgeeiENSRNOG00000013000.
GenevisibleiP42123. RN.

Interactioni

Subunit structurei

Homotetramer.

GO - Molecular functioni

  • identical protein binding Source: RGD
  • kinase binding Source: RGD

Protein-protein interaction databases

BioGridi246687. 2 interactors.
IntActiP42123. 2 interactors.
MINTiMINT-4568048.
STRINGi10116.ENSRNOP00000017965.

Structurei

3D structure databases

ProteinModelPortaliP42123.
SMRiP42123.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the LDH/MDH superfamily. LDH family.Curated

Phylogenomic databases

eggNOGiKOG1495. Eukaryota.
COG0039. LUCA.
GeneTreeiENSGT00550000074541.
HOGENOMiHOG000213793.
HOVERGENiHBG000462.
InParanoidiP42123.
KOiK00016.
OMAiRQPGQTR.
OrthoDBiEOG091G0HME.
PhylomeDBiP42123.
TreeFamiTF314963.

Family and domain databases

HAMAPiMF_00488. Lactate_dehydrog. 1 hit.
InterProiView protein in InterPro
IPR001557. L-lactate/malate_DH.
IPR011304. L-lactate_DH.
IPR018177. L-lactate_DH_AS.
IPR022383. Lactate/malate_DH_C.
IPR001236. Lactate/malate_DH_N.
IPR015955. Lactate_DH/Glyco_Ohase_4_C.
IPR016040. NAD(P)-bd_dom.
PfamiView protein in Pfam
PF02866. Ldh_1_C. 1 hit.
PF00056. Ldh_1_N. 1 hit.
PIRSFiPIRSF000102. Lac_mal_DH. 1 hit.
PRINTSiPR00086. LLDHDRGNASE.
SUPFAMiSSF51735. SSF51735. 1 hit.
SSF56327. SSF56327. 1 hit.
TIGRFAMsiTIGR01771. L-LDH-NAD. 1 hit.
PROSITEiView protein in PROSITE
PS00064. L_LDH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42123-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATLKEKLIA PVADDETAVP NNKITVVGVG QVGMACAISI LGKSLADELA
60 70 80 90 100
LVDVLEDKLK GEMMDLQHGS LFLQTPKIVA DKDYSVTANS KIVVVTAGVR
110 120 130 140 150
QQEGESRLNL VQRNVNVFKF IIPQIVKYSP DCTIIVVSNP VDILTYVTWK
160 170 180 190 200
LSGLPKHRVI GSGCNLDSAR FRYLMAEKLG IHPSSCHGWI LGEHGDSSVA
210 220 230 240 250
VWSGVNVAGV SLQELNPEMG TDNDSENWKE VHKMVVDSAY EVIKLKGYTN
260 270 280 290 300
WAIGLSVADL IESMLKNLSR IHPVSTMVKG MYGIENEVFL SLPCILNARG
310 320 330
LTSVINQKLK DDEVAQLRKS ADTLWDIQKD LKDL
Length:334
Mass (Da):36,612
Last modified:January 23, 2007 - v2
Checksum:iAE9DE0AF5194CDE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07181 mRNA. Translation: AAA50439.1.
BC059149 mRNA. Translation: AAH59149.1.
PIRiI62761.
RefSeqiNP_001303262.1. NM_001316333.1.
NP_001303263.1. NM_001316334.1.
NP_036727.1. NM_012595.2.
UniGeneiRn.1785.

Genome annotation databases

EnsembliENSRNOT00000017965; ENSRNOP00000017965; ENSRNOG00000013000.
GeneIDi24534.
KEGGirno:24534.
UCSCiRGD:2997. rat.

Similar proteinsi

Entry informationi

Entry nameiLDHB_RAT
AccessioniPrimary (citable) accession number: P42123
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: August 30, 2017
This is version 146 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families