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Protein

DNA nucleotidylexotransferase

Gene

dntt

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).By similarity

Cofactori

Mg2+By similarityNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi339 – 3391MagnesiumBy similarity
Metal bindingi341 – 3411MagnesiumBy similarity
Metal bindingi431 – 4311MagnesiumBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Biological processi

Terminal addition

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.31)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name:
TDT
Short name:
Terminal transferase
Gene namesi
Name:dntt
Synonyms:tdt
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252658. dntt.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 507507DNA nucleotidylexotransferasePRO_0000218797Add
BLAST

Expressioni

Tissue specificityi

Found in the thymus and not in the spleen, kidney, intestine, or liver.

Developmental stagei

During ontogeny TDT appears in significant amounts in the thymus of tadpoles at metamorphic climax but little in the earlier midlarval stages.

Structurei

3D structure databases

ProteinModelPortaliP42118.
SMRiP42118. Positions 23-125, 145-507.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 12498BRCTPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni254 – 2585Involved in DNA bindingBy similarity
Regioni329 – 3346Deoxynucleoside triphosphate bindingBy similarity
Regioni338 – 3414Deoxynucleoside triphosphate bindingBy similarity
Regioni446 – 4472Deoxynucleoside triphosphate bindingBy similarity

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated
Contains 1 BRCT domain.PROSITE-ProRule annotation

Phylogenomic databases

HOVERGENiHBG003670.
KOiK00977.

Family and domain databases

CDDicd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P42118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPLSQSALV PLRKKAKMAP ISQSFCQHNV KFKEIVLFLV ERKMGSSRRT
60 70 80 90 100
FLMELARKRG FQTEIELSDS VTHIVAENNS GAEVLEWLQS KKLGFTVKTH
110 120 130 140 150
ILDISWFTEC MEAGRPVEIQ NRHLLPVQQD CSANFNPPLS SSCVQVSQYA
160 170 180 190 200
CQRCTTLQDT NRIFTDAFDI LAEHFEFCEN KGRTVAFLRA SSLIKSLPFP
210 220 230 240 250
ITAMKELEGL PWLGDQMKGI IEEILEEGKS YKVLEVMNEE RYKSFKQFTS
260 270 280 290 300
VFGVGLKTSD KWFRMGFRTL EEIKNEKELK LTKMQKCGLL YYEDITSYVS
310 320 330 340 350
RAEAETTEQL IKSIVWKFVP DAIVTLTGGF RRGKKKGHDV DILITCARKG
360 370 380 390 400
KEKNILHNTM SVLKNRGLLL FYNIIESTFD ETKLPSRHVD ALDHFQKCFT
410 420 430 440 450
ILKLPKRQMD IGNIIDPHEC ERKNWKAVRL DLVITPYEQY PYALLGWTGS
460 470 480 490 500
RQFERDLRRY ATHEKRMMLD NHGLYDKTKN NFLKANNEED IFKQLGLDYL

EPWERNA
Length:507
Mass (Da):58,846
Last modified:November 1, 1995 - v1
Checksum:i4B57F91C96E8B6D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07803 mRNA. Translation: AAA18493.1.
PIRiI51658.
RefSeqiNP_001079251.1. NM_001085782.1.
UniGeneiXl.969.

Genome annotation databases

GeneIDi378525.
KEGGixla:378525.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07803 mRNA. Translation: AAA18493.1.
PIRiI51658.
RefSeqiNP_001079251.1. NM_001085782.1.
UniGeneiXl.969.

3D structure databases

ProteinModelPortaliP42118.
SMRiP42118. Positions 23-125, 145-507.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi378525.
KEGGixla:378525.

Organism-specific databases

CTDi1791.
XenbaseiXB-GENE-6252658. dntt.

Phylogenomic databases

HOVERGENiHBG003670.
KOiK00977.

Family and domain databases

CDDicd00141. NT_POLXc. 1 hit.
Gene3Di1.10.150.110. 1 hit.
3.30.210.10. 1 hit.
3.40.50.10190. 1 hit.
InterProiIPR001357. BRCT_dom.
IPR002054. DNA-dir_DNA_pol_X.
IPR019843. DNA_pol-X_BS.
IPR010996. DNA_pol_b-like_N.
IPR028207. DNA_pol_B_palm_palm.
IPR018944. DNA_pol_lambd_fingers_domain.
IPR022312. DNA_pol_X.
IPR027421. DNA_pol_X_lyase_dom.
IPR029398. PolB_thumb.
IPR027292. TdT.
IPR001726. TdT/Mu.
[Graphical view]
PfamiPF14792. DNA_pol_B_palm. 1 hit.
PF14791. DNA_pol_B_thumb. 1 hit.
PF10391. DNA_pol_lambd_f. 1 hit.
PF14716. HHH_8. 1 hit.
[Graphical view]
PIRSFiPIRSF000817. DNA_NT. 1 hit.
PIRSF501175. TDT. 1 hit.
PRINTSiPR00869. DNAPOLX.
PR00871. DNAPOLXTDT.
SMARTiSM00292. BRCT. 1 hit.
SM00483. POLXc. 1 hit.
[Graphical view]
SUPFAMiSSF47802. SSF47802. 1 hit.
SSF52113. SSF52113. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS00522. DNA_POLYMERASE_X. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTDT_XENLA
AccessioniPrimary (citable) accession number: P42118
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.