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Protein

DNA nucleotidylexotransferase

Gene

dntt

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Template-independent DNA polymerase which catalyzes the random addition of deoxynucleoside 5'-triphosphate to the 3'-end of a DNA initiator. One of the in vivo functions of this enzyme is the addition of nucleotides at the junction (N region) of rearranged Ig heavy chain and T-cell receptor gene segments during the maturation of B- and T-cells.By similarity

Catalytic activityi

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).By similarity

Cofactori

Mg2+By similarityNote: Can also utilize other divalent cations, such as Mn2+ and Co2+ (in vitro).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi339MagnesiumBy similarity1
Metal bindingi341MagnesiumBy similarity1
Metal bindingi431MagnesiumBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processTerminal addition
LigandMagnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
DNA nucleotidylexotransferase (EC:2.7.7.31)
Alternative name(s):
Terminal addition enzyme
Terminal deoxynucleotidyltransferase
Short name:
TDT
Short name:
Terminal transferase
Gene namesi
Name:dntt
Synonyms:tdt
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6252658 dntt

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002187971 – 507DNA nucleotidylexotransferaseAdd BLAST507

Proteomic databases

PRIDEiP42118

Expressioni

Tissue specificityi

Found in the thymus and not in the spleen, kidney, intestine, or liver.

Developmental stagei

During ontogeny TDT appears in significant amounts in the thymus of tadpoles at metamorphic climax but little in the earlier midlarval stages.

Structurei

3D structure databases

ProteinModelPortaliP42118
SMRiP42118
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini27 – 124BRCTPROSITE-ProRule annotationAdd BLAST98

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni254 – 258Involved in DNA bindingBy similarity5
Regioni329 – 334Deoxynucleoside triphosphate bindingBy similarity6
Regioni338 – 341Deoxynucleoside triphosphate bindingBy similarity4
Regioni446 – 447Deoxynucleoside triphosphate bindingBy similarity2

Sequence similaritiesi

Belongs to the DNA polymerase type-X family.Curated

Phylogenomic databases

HOVERGENiHBG003670
KOiK00977

Family and domain databases

CDDicd00027 BRCT, 1 hit
cd00141 NT_POLXc, 1 hit
Gene3Di1.10.150.110, 1 hit
3.30.210.10, 1 hit
3.40.50.10190, 1 hit
InterProiView protein in InterPro
IPR001357 BRCT_dom
IPR036420 BRCT_dom_sf
IPR002054 DNA-dir_DNA_pol_X
IPR019843 DNA_pol-X_BS
IPR010996 DNA_pol_b-like_N
IPR028207 DNA_pol_B_palm_palm
IPR018944 DNA_pol_lambd_fingers_domain
IPR027421 DNA_pol_lamdba_lyase_dom_sf
IPR037160 DNA_Pol_thumb_sf
IPR022312 DNA_pol_X
IPR029398 PolB_thumb
IPR027292 TdT
IPR001726 TdT/Mu
PfamiView protein in Pfam
PF14792 DNA_pol_B_palm, 1 hit
PF14791 DNA_pol_B_thumb, 1 hit
PF10391 DNA_pol_lambd_f, 1 hit
PF14716 HHH_8, 1 hit
PIRSFiPIRSF000817 DNA_NT, 1 hit
PIRSF501175 TDT, 1 hit
PRINTSiPR00869 DNAPOLX
PR00871 DNAPOLXTDT
SMARTiView protein in SMART
SM00292 BRCT, 1 hit
SM00483 POLXc, 1 hit
SUPFAMiSSF47802 SSF47802, 1 hit
SSF52113 SSF52113, 1 hit
PROSITEiView protein in PROSITE
PS50172 BRCT, 1 hit
PS00522 DNA_POLYMERASE_X, 1 hit

Sequencei

Sequence statusi: Complete.

P42118-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNPLSQSALV PLRKKAKMAP ISQSFCQHNV KFKEIVLFLV ERKMGSSRRT
60 70 80 90 100
FLMELARKRG FQTEIELSDS VTHIVAENNS GAEVLEWLQS KKLGFTVKTH
110 120 130 140 150
ILDISWFTEC MEAGRPVEIQ NRHLLPVQQD CSANFNPPLS SSCVQVSQYA
160 170 180 190 200
CQRCTTLQDT NRIFTDAFDI LAEHFEFCEN KGRTVAFLRA SSLIKSLPFP
210 220 230 240 250
ITAMKELEGL PWLGDQMKGI IEEILEEGKS YKVLEVMNEE RYKSFKQFTS
260 270 280 290 300
VFGVGLKTSD KWFRMGFRTL EEIKNEKELK LTKMQKCGLL YYEDITSYVS
310 320 330 340 350
RAEAETTEQL IKSIVWKFVP DAIVTLTGGF RRGKKKGHDV DILITCARKG
360 370 380 390 400
KEKNILHNTM SVLKNRGLLL FYNIIESTFD ETKLPSRHVD ALDHFQKCFT
410 420 430 440 450
ILKLPKRQMD IGNIIDPHEC ERKNWKAVRL DLVITPYEQY PYALLGWTGS
460 470 480 490 500
RQFERDLRRY ATHEKRMMLD NHGLYDKTKN NFLKANNEED IFKQLGLDYL

EPWERNA
Length:507
Mass (Da):58,846
Last modified:November 1, 1995 - v1
Checksum:i4B57F91C96E8B6D1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U07803 mRNA Translation: AAA18493.1
PIRiI51658
RefSeqiNP_001079251.1, NM_001085782.1
UniGeneiXl.969

Genome annotation databases

GeneIDi378525
KEGGixla:378525

Similar proteinsi

Entry informationi

Entry nameiTDT_XENLA
AccessioniPrimary (citable) accession number: P42118
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health