P42117 (NDUA3_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NADH-ubiquinone oxidoreductase 9.5 kDa subunit

Alternative name(s):
Complex I-9.5kD
Short name=CI-9.5
Ubiquinone-binding protein

Gene names

ORF Names:

NCU00670

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Pezizomycotina › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora

Protein attributes

Sequence length	82 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This subunit binds ubiquinone.
Subunit structure	Complex I is composed of about 40 different subunits.
Subcellular location	Mitochondrion inner membrane; Single-pass membrane protein.
Sequence similarities	Belongs to the complex I NDUFA3 subunit family.
Sequence caution	The sequence EAA36574.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Electron transport Respiratory chain Transport
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell respiratory chain Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 82	81	NADH-ubiquinone oxidoreductase 9.5 kDa subunit		PRO_0000118795
Regions
	Transmembrane	25 – 43	19	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P42117 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F7EC76B3246C4E77	FASTA	82	9,262
	10 20 30 40 50 60 MSGTIPHFWA QPFRYIRWSA REKPAYFYSC VIAGLGPVFL TVVPPVRKYF GDVNPAPIPV 70 80 TYPIPTGPRK QLTGYDDDTE EA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of the 9.5-kDa ubiquinone-binding protein of NADH:ubiquinone oxidoreductase (complex I) from Neurospora crassa." Heinrich H., Azevedo J.E., Werner S. Biochemistry 31:11420-11424(1992) [PubMed: 1445879] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-7. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed: 12712197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"Identification of the ubiquinone-binding site of NADH:ubiquinone oxidoreductase (complex I) from Neurospora crassa." Heinrich H., Werner S. Biochemistry 31:11413-11419(1992) [PubMed: 1445878] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S49807 mRNA. Translation: AAB24322.1. AABX02000002 Genomic DNA. Translation: EAA36574.2. Sequence problems.
PIR	A44210.
RefSeq	XP_965810.2. XM_960717.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000000869; EFNCRP00000000869; EFNCRG00000000869.
GeneID	3881935.
KEGG	ncr:NCU00670.
NMPDR	fig|5141.1.peg.2783.
Phylogenomic databases
eggNOG	fuNOG12045.
GeneTree	EFGT00050000004887.
OrthoDB	EOG46MFVH.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

NDUA3_NEUCR

Accession

Primary (citable) accession number: P42117
Secondary accession number(s): Q7SHY3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	October 19, 2011
This is version 77 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program