Reviewed, UniProtKB/Swiss-Prot P42113 (ASNH_BACSU)

Names and origin

Protein names

Recommended name:
    Asparagine synthetase [glutamine-hydrolyzing] 2
    EC=6.3.5.4

Gene names

Name:	asnH
Synonyms:	yxaN
Ordered Locus Names:	BSU39920
ORF Names:	S14NR, VE7AR

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	747 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.
Sequence similarities	Belongs to the asparagine synthetase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 747	747	Asparagine synthetase [glutamine-hydrolyzing] 2		PRO_0000056933
Regions
	Domain	2 – 218	217	Glutamine amidotransferase type-2
Sites
	Active site	2	1	For GATase activity By similarity
Experimental info
	Sequence conflict	185	1	W → C in BAA21593. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42113-1 [UniParc]. Last modified July 28, 2009. Version 2. Checksum: E1447AC0B72922CE	FASTA	747	85,932
	10 20 30 40 50 60 MCGLAGIINL AAPRSQECTF HILKGMADAI SYRGPDDEQY HIDSKVGFAF RRLSILDLVN 70 80 90 100 110 120 GQQPFLNEDG SIVVMVNGEI YNYKELKASL HNHMFKTTSD CEVIVHLYEE KGIGFVDDII 130 140 150 160 170 180 GMFSIAIWDK NKNKVFLVRD RFGIKPLFYT ELKHELIFAS EIKSLFSHPH CPRQFNWKEA 190 200 210 220 230 240 LSDIWLSGEA ASNHKETTSF FVNIQNLDAG HYLEINLTTN ERKTASYWSL QDILLRQGYR 250 260 270 280 290 300 ENLHPDDLIE GYRELLADSV HRCLQSDVEV GLFLSGGIDS AAVAHFAAEK QDLHTFTVLS 310 320 330 340 350 360 QSTFTNEDAK YAHWLAKDLH LPNHQVLYQL GNDELLQPES YKHLLWICET PFCGPEQLYK 370 380 390 400 410 420 FHLHKYAKAI RPNLKVMLTG QGSDEFNGGY STTLSPAENP SWEGFIESVN TMEMNRLHRL 430 440 450 460 470 480 QGNIFRVWEE HFGLSPINLS YLKSNDSSQA DPWQSYVLTK YRDLQMYNCW HEDRIAAANH 490 500 510 520 530 540 IENRVPFLDH RLVEWVCGIP DGLRKDLLWD KSVLRKSLTN ELHTSYTHRP KVPFFYGKDV 550 560 570 580 590 600 RYTHKMMFHL LKKNNYQLIE EAFSHSDASS IIQVEHIHAI MTYLEDDPEF TNFEFLLRLV 610 620 630 640 650 660 NMGLLSKMTK ETPSVQLDIT SHLESITIKD WHSQEGDIAS RLNISANKCE GQDILALNPG 670 680 690 700 710 720 VTLLRPESDS EHCIYIAEEG FIQFIVSEED VGAWLHILCD INGKDTLHTI LDRHGVSLEE 730 740 VAKYIQEAIE HNIILIKQKN LPEGAYR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome between the gnt and iol operons." Yoshida K., Seki S., Fujimura M., Miwa Y., Fujita Y. DNA Res. 2:61-69(1995) [PubMed: 7584049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / BGSC1A1.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 185.
[4]	"Three asparagine synthetase genes of Bacillus subtilis." Yoshida K., Fujita Y., Ehrlich S.D. J. Bacteriol. 181:6081-6091(1999) [PubMed: 10498721] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AB005554 Genomic DNA. Translation: BAA21593.1. AL009126 Genomic DNA. Translation: CAB16028.2.
PIR	A69591.
RefSeq	NP_391871.2.
3D structure databases
HSSP	HSSP built from PDB template 1CT9 based on UniProtKB P22106.
ModBase	Search...
Genome annotation databases
GeneID	937677.
GenomeReviews	Gene locus BSU39920 in contig AL009126_GR.
KEGG	bsu:BSU39920.
NMPDR	fig|224308.1.peg.3997.
Organism-specific databases
SubtiList	BG11116. asnH. [Micado]
CMR	Search...
Phylogenomic databases
OMA	NISANKC.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU3988-MON.
BRENDA	6.3.5.4. 150.
Family and domain databases
InterPro	IPR006426. Asn_synth_AEB. IPR001962. Asn_synthase. IPR000583. GATase_2. IPR017932. GATase_II. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF00733. Asn_synthase. 1 hit. PF00310. GATase_2. 1 hit. [Graphical view]
TIGRFAMs	TIGR01536. asn_synth_AEB. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNH_BACSU

Accession

Primary (citable) accession number: P42113

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	July 28, 2009
Last modified:	November 3, 2009
This is version 72 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)