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Protein

Asparagine synthetase [glutamine-hydrolyzing] 2

Gene

asnH

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathwayi: L-asparagine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route).
Proteins known to be involved in this subpathway in this organism are:
  1. Asparagine synthetase [glutamine-hydrolyzing] 2 (asnH), Asparagine synthetase [glutamine-hydrolyzing] 3 (asnO), Asparagine synthetase [glutamine-hydrolyzing] 1 (asnB)
This subpathway is part of the pathway L-asparagine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-asparagine from L-aspartate (L-Gln route), the pathway L-asparagine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei2For GATase activityBy similarity1
Binding sitei100GlutamineBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi395 – 396ATPBy similarity2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Amino-acid biosynthesis, Asparagine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBSUB:BSU39920-MONOMER.
UniPathwayiUPA00134; UER00195.

Names & Taxonomyi

Protein namesi
Recommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2 (EC:6.3.5.4)
Gene namesi
Name:asnH
Synonyms:yxaN
Ordered Locus Names:BSU39920
ORF Names:S14NR, VE7AR
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000569332 – 747Asparagine synthetase [glutamine-hydrolyzing] 2Add BLAST746

Proteomic databases

PaxDbiP42113.
PRIDEiP42113.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021526.

Structurei

3D structure databases

ProteinModelPortaliP42113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 218Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd BLAST217

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni52 – 56Glutamine bindingBy similarity5
Regioni77 – 79Glutamine bindingBy similarity3

Sequence similaritiesi

Belongs to the asparagine synthetase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4105CAQ. Bacteria.
COG0367. LUCA.
HOGENOMiHOG000155832.
InParanoidiP42113.
KOiK01953.
OMAiEDAKYAH.
PhylomeDBiP42113.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCGLAGIINL AAPRSQECTF HILKGMADAI SYRGPDDEQY HIDSKVGFAF
60 70 80 90 100
RRLSILDLVN GQQPFLNEDG SIVVMVNGEI YNYKELKASL HNHMFKTTSD
110 120 130 140 150
CEVIVHLYEE KGIGFVDDII GMFSIAIWDK NKNKVFLVRD RFGIKPLFYT
160 170 180 190 200
ELKHELIFAS EIKSLFSHPH CPRQFNWKEA LSDIWLSGEA ASNHKETTSF
210 220 230 240 250
FVNIQNLDAG HYLEINLTTN ERKTASYWSL QDILLRQGYR ENLHPDDLIE
260 270 280 290 300
GYRELLADSV HRCLQSDVEV GLFLSGGIDS AAVAHFAAEK QDLHTFTVLS
310 320 330 340 350
QSTFTNEDAK YAHWLAKDLH LPNHQVLYQL GNDELLQPES YKHLLWICET
360 370 380 390 400
PFCGPEQLYK FHLHKYAKAI RPNLKVMLTG QGSDEFNGGY STTLSPAENP
410 420 430 440 450
SWEGFIESVN TMEMNRLHRL QGNIFRVWEE HFGLSPINLS YLKSNDSSQA
460 470 480 490 500
DPWQSYVLTK YRDLQMYNCW HEDRIAAANH IENRVPFLDH RLVEWVCGIP
510 520 530 540 550
DGLRKDLLWD KSVLRKSLTN ELHTSYTHRP KVPFFYGKDV RYTHKMMFHL
560 570 580 590 600
LKKNNYQLIE EAFSHSDASS IIQVEHIHAI MTYLEDDPEF TNFEFLLRLV
610 620 630 640 650
NMGLLSKMTK ETPSVQLDIT SHLESITIKD WHSQEGDIAS RLNISANKCE
660 670 680 690 700
GQDILALNPG VTLLRPESDS EHCIYIAEEG FIQFIVSEED VGAWLHILCD
710 720 730 740
INGKDTLHTI LDRHGVSLEE VAKYIQEAIE HNIILIKQKN LPEGAYR
Length:747
Mass (Da):85,932
Last modified:July 28, 2009 - v2
Checksum:iE1447AC0B72922CE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti185W → C in BAA21593 (PubMed:7584049).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005554 Genomic DNA. Translation: BAA21593.1.
AL009126 Genomic DNA. Translation: CAB16028.2.
PIRiA69591.
RefSeqiNP_391871.2. NC_000964.3.
WP_003244103.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16028; CAB16028; BSU39920.
GeneIDi937677.
KEGGibsu:BSU39920.
PATRICi18980052. VBIBacSub10457_4188.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005554 Genomic DNA. Translation: BAA21593.1.
AL009126 Genomic DNA. Translation: CAB16028.2.
PIRiA69591.
RefSeqiNP_391871.2. NC_000964.3.
WP_003244103.1. NZ_JNCM01000034.1.

3D structure databases

ProteinModelPortaliP42113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021526.

Proteomic databases

PaxDbiP42113.
PRIDEiP42113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16028; CAB16028; BSU39920.
GeneIDi937677.
KEGGibsu:BSU39920.
PATRICi18980052. VBIBacSub10457_4188.

Phylogenomic databases

eggNOGiENOG4105CAQ. Bacteria.
COG0367. LUCA.
HOGENOMiHOG000155832.
InParanoidiP42113.
KOiK01953.
OMAiEDAKYAH.
PhylomeDBiP42113.

Enzyme and pathway databases

UniPathwayiUPA00134; UER00195.
BioCyciBSUB:BSU39920-MONOMER.

Family and domain databases

CDDicd01991. Asn_Synthase_B_C. 1 hit.
cd00712. AsnB. 1 hit.
Gene3Di3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProiIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR033738. AsnB_N.
IPR017932. GATase_2_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
SUPFAMiSSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01536. asn_synth_AEB. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiASNH_BACSU
AccessioniPrimary (citable) accession number: P42113
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 28, 2009
Last modified: October 5, 2016
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.