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P42113 (ASNH_BACSU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine synthetase [glutamine-hydrolyzing] 2

EC=6.3.5.4
Gene names
Name:asnH
Synonyms:yxaN
Ordered Locus Names:BSU39920
ORF Names:S14NR, VE7AR
OrganismBacillus subtilis (strain 168) [Reference proteome] [HAMAP]
Taxonomic identifier224308 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

Protein attributes

Sequence length747 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

ATP + L-aspartate + L-glutamine + H2O = AMP + diphosphate + L-asparagine + L-glutamate.

Pathway

Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (L-Gln route): step 1/1.

Sequence similarities

Belongs to the asparagine synthetase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 747746Asparagine synthetase [glutamine-hydrolyzing] 2
PRO_0000056933

Regions

Domain2 – 218217Glutamine amidotransferase type-2
Nucleotide binding395 – 3962ATP By similarity
Region52 – 565Glutamine binding By similarity
Region77 – 793Glutamine binding By similarity

Sites

Active site21For GATase activity By similarity
Binding site1001Glutamine By similarity

Experimental info

Sequence conflict1851W → C in BAA21593. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P42113 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: E1447AC0B72922CE

FASTA74785,932
        10         20         30         40         50         60 
MCGLAGIINL AAPRSQECTF HILKGMADAI SYRGPDDEQY HIDSKVGFAF RRLSILDLVN 

        70         80         90        100        110        120 
GQQPFLNEDG SIVVMVNGEI YNYKELKASL HNHMFKTTSD CEVIVHLYEE KGIGFVDDII 

       130        140        150        160        170        180 
GMFSIAIWDK NKNKVFLVRD RFGIKPLFYT ELKHELIFAS EIKSLFSHPH CPRQFNWKEA 

       190        200        210        220        230        240 
LSDIWLSGEA ASNHKETTSF FVNIQNLDAG HYLEINLTTN ERKTASYWSL QDILLRQGYR 

       250        260        270        280        290        300 
ENLHPDDLIE GYRELLADSV HRCLQSDVEV GLFLSGGIDS AAVAHFAAEK QDLHTFTVLS 

       310        320        330        340        350        360 
QSTFTNEDAK YAHWLAKDLH LPNHQVLYQL GNDELLQPES YKHLLWICET PFCGPEQLYK 

       370        380        390        400        410        420 
FHLHKYAKAI RPNLKVMLTG QGSDEFNGGY STTLSPAENP SWEGFIESVN TMEMNRLHRL 

       430        440        450        460        470        480 
QGNIFRVWEE HFGLSPINLS YLKSNDSSQA DPWQSYVLTK YRDLQMYNCW HEDRIAAANH 

       490        500        510        520        530        540 
IENRVPFLDH RLVEWVCGIP DGLRKDLLWD KSVLRKSLTN ELHTSYTHRP KVPFFYGKDV 

       550        560        570        580        590        600 
RYTHKMMFHL LKKNNYQLIE EAFSHSDASS IIQVEHIHAI MTYLEDDPEF TNFEFLLRLV 

       610        620        630        640        650        660 
NMGLLSKMTK ETPSVQLDIT SHLESITIKD WHSQEGDIAS RLNISANKCE GQDILALNPG 

       670        680        690        700        710        720 
VTLLRPESDS EHCIYIAEEG FIQFIVSEED VGAWLHILCD INGKDTLHTI LDRHGVSLEE 

       730        740 
VAKYIQEAIE HNIILIKQKN LPEGAYR 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of a 36-kb region of the Bacillus subtilis genome between the gnt and iol operons."
Yoshida K., Seki S., Fujimura M., Miwa Y., Fujita Y.
DNA Res. 2:61-69(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 168 / BGSC1A1.
[2]"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis."
Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. expand/collapse author list , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A.
Nature 390:249-256(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 168.
[3]"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later."
Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.
Microbiology 155:1758-1775(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: SEQUENCE REVISION TO 185.
[4]"Three asparagine synthetase genes of Bacillus subtilis."
Yoshida K., Fujita Y., Ehrlich S.D.
J. Bacteriol. 181:6081-6091(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB005554 Genomic DNA. Translation: BAA21593.1.
AL009126 Genomic DNA. Translation: CAB16028.2.
PIRA69591.
RefSeqNP_391871.2. NC_000964.3.

3D structure databases

ProteinModelPortalP42113.
SMRP42113. Positions 2-542.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224308.BSU39920.

Proteomic databases

PaxDbP42113.
PRIDEP42113.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaCAB16028; CAB16028; BSU39920.
GeneID937677.
KEGGbsu:BSU39920.
PATRIC18980052. VBIBacSub10457_4188.

Organism-specific databases

GenoListBSU39920. [Micado]

Phylogenomic databases

eggNOGCOG0367.
HOGENOMHOG000155832.
KOK01953.
OrthoDBEOG6P8TM1.
PhylomeDBP42113.

Enzyme and pathway databases

BioCycBSUB:BSU39920-MONOMER.
UniPathwayUPA00134; UER00195.

Family and domain databases

Gene3D3.40.50.620. 2 hits.
3.60.20.10. 1 hit.
InterProIPR006426. Asn_synth_AEB.
IPR001962. Asn_synthase.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00733. Asn_synthase. 1 hit.
PF13537. GATase_7. 1 hit.
[Graphical view]
PIRSFPIRSF001589. Asn_synthetase_glu-h. 1 hit.
SUPFAMSSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01536. asn_synth_AEB. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASNH_BACSU
AccessionPrimary (citable) accession number: P42113
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 28, 2009
Last modified: July 9, 2014
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Bacillus subtilis

Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList