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Protein

Quercetin 2,3-dioxygenase

Gene

qdoI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Performs the first step in the degradation of the flavonoid quercetin by a dioxygenase reaction. The enzyme catalyzes the cleavage of the O-heteroaromatic ring of the flavonol quercetin yielding the depside 2-protocatechuoyl-phloroglucinol carboxylic acid and carbon monoxide. This involves the remarkable dioxygenolytic cleavage of two carbon-carbon bonds.1 Publication

Catalytic activityi

Quercetin + O2 = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H+.

Cofactori

Fe2+1 PublicationNote: Binds 2 Fe2+ ions per subunit.1 Publication

Kineticsi

  1. KM=3.8 µM for quercetin1 Publication
  1. Vmax=2 µmol/min/mg enzyme1 Publication

Pathwayi: quercetin degradation

This protein is involved in the pathway quercetin degradation, which is part of Flavonoid metabolism.
View all proteins of this organism that are known to be involved in the pathway quercetin degradation and in Flavonoid metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi62 – 621Iron 1
Metal bindingi64 – 641Iron 1
Metal bindingi69 – 691Iron 1
Metal bindingi103 – 1031Iron 1
Metal bindingi234 – 2341Iron 2
Metal bindingi236 – 2361Iron 2
Metal bindingi241 – 2411Iron 2
Metal bindingi275 – 2751Iron 2

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Dioxygenase, Oxidoreductase

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBSUB:BSU39980-MONOMER.
BRENDAi1.13.11.24. 658.
SABIO-RKP42106.
UniPathwayiUPA00724.

Names & Taxonomyi

Protein namesi
Recommended name:
Quercetin 2,3-dioxygenase (EC:1.13.11.24)
Short name:
Quercetinase
Alternative name(s):
Flavonol 2,4-dioxygenase
Gene namesi
Name:qdoI
Synonyms:yxaG
Ordered Locus Names:BSU39980
ORF Names:S14G
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 337337Quercetin 2,3-dioxygenasePRO_0000097135Add
BLAST

Proteomic databases

PaxDbiP42106.

Interactioni

Subunit structurei

Homodimer.

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021561.

Structurei

Secondary structure

1
337
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi11 – 133Combined sources
Beta strandi16 – 183Combined sources
Beta strandi23 – 286Combined sources
Beta strandi31 – 377Combined sources
Helixi39 – 424Combined sources
Beta strandi44 – 5310Combined sources
Beta strandi58 – 636Combined sources
Beta strandi69 – 768Combined sources
Beta strandi78 – 825Combined sources
Beta strandi85 – 895Combined sources
Beta strandi94 – 974Combined sources
Beta strandi103 – 1075Combined sources
Beta strandi112 – 1198Combined sources
Helixi125 – 1306Combined sources
Beta strandi131 – 1333Combined sources
Helixi153 – 1553Combined sources
Beta strandi183 – 1853Combined sources
Beta strandi188 – 1903Combined sources
Beta strandi195 – 2006Combined sources
Beta strandi203 – 2097Combined sources
Helixi211 – 2133Combined sources
Turni214 – 2163Combined sources
Beta strandi219 – 2257Combined sources
Beta strandi240 – 2489Combined sources
Beta strandi250 – 2545Combined sources
Beta strandi257 – 2615Combined sources
Beta strandi266 – 2694Combined sources
Beta strandi275 – 2795Combined sources
Beta strandi281 – 29313Combined sources
Turni294 – 2963Combined sources
Helixi297 – 3026Combined sources
Beta strandi303 – 3053Combined sources
Beta strandi308 – 3103Combined sources
Helixi320 – 3256Combined sources
Helixi327 – 3293Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y3TX-ray2.40A/B1-337[»]
2H0VX-ray2.60A/B1-337[»]
ProteinModelPortaliP42106.
SMRiP42106. Positions 3-337.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42106.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 148148Cupin 1Add
BLAST
Regioni177 – 333157Cupin 2Add
BLAST

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1917. LUCA.
HOGENOMiHOG000087868.
InParanoidiP42106.
KOiK07155.
OMAiDFLHVPA.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR013096. Cupin_2.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.

Sequencei

Sequence statusi: Complete.

P42106-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKTLCTHSLP KEKMPYLLRS GEGERYLFGR QVATVMANGR STGDLFEIVL
60 70 80 90 100
LSGGKGDAFP LHVHKDTHEG ILVLDGKLEL TLDGERYLLI SGDYANIPAG
110 120 130 140 150
TPHSYRMQSH RTRLVSYTMK GNVAHLYSVI GNPYDHAEHP PYASEEVSNE
160 170 180 190 200
RFAEAAAVAD IVFLDEAKPA CSAKLAELTE LPDGAVPYVL ESGEGDRLLT
210 220 230 240 250
GDQLHRIVAA QKNTDGQFIV VSSEGPKGDR IVDHYHEYHT ETFYCLEGQM
260 270 280 290 300
TMWTDGQEIQ LNPGDFLHVP ANTVHSYRLD SHYTKMVGVL VPGLFEPFFR
310 320 330
TLGDPYEGHI FPCEPQALRF DRILQNIEAL DLKVMKP
Length:337
Mass (Da):37,600
Last modified:July 28, 2009 - v2
Checksum:iA91148E6272AE64C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti160 – 1601D → T in BAA21586 (PubMed:7584049).Curated
Sequence conflicti314 – 3141E → K in BAA21586 (PubMed:7584049).Curated

Mass spectrometryi

Molecular mass is 38658 Da from positions 1 - 337. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005554 Genomic DNA. Translation: BAA21586.1.
AL009126 Genomic DNA. Translation: CAB16035.2.
PIRiF70071.
RefSeqiNP_391878.2. NC_000964.3.
WP_003243000.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB16035; CAB16035; BSU39980.
GeneIDi937689.
KEGGibsu:BSU39980.
PATRICi18980064. VBIBacSub10457_4194.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005554 Genomic DNA. Translation: BAA21586.1.
AL009126 Genomic DNA. Translation: CAB16035.2.
PIRiF70071.
RefSeqiNP_391878.2. NC_000964.3.
WP_003243000.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Y3TX-ray2.40A/B1-337[»]
2H0VX-ray2.60A/B1-337[»]
ProteinModelPortaliP42106.
SMRiP42106. Positions 3-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021561.

Proteomic databases

PaxDbiP42106.

Protocols and materials databases

DNASUi937689.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB16035; CAB16035; BSU39980.
GeneIDi937689.
KEGGibsu:BSU39980.
PATRICi18980064. VBIBacSub10457_4194.

Phylogenomic databases

eggNOGiCOG1917. LUCA.
HOGENOMiHOG000087868.
InParanoidiP42106.
KOiK07155.
OMAiDFLHVPA.

Enzyme and pathway databases

UniPathwayiUPA00724.
BioCyciBSUB:BSU39980-MONOMER.
BRENDAi1.13.11.24. 658.
SABIO-RKP42106.

Miscellaneous databases

EvolutionaryTraceiP42106.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR013096. Cupin_2.
IPR014710. RmlC-like_jellyroll.
IPR011051. RmlC_Cupin.
[Graphical view]
PfamiPF07883. Cupin_2. 2 hits.
[Graphical view]
SUPFAMiSSF51182. SSF51182. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiQDOI_BACSU
AccessioniPrimary (citable) accession number: P42106
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: July 28, 2009
Last modified: September 7, 2016
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.