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Protein

Zona pellucida sperm-binding protein 3

Gene

ZP3

Organism
Sus scrofa (Pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

The mammalian zona pellucida, which mediates species-specific sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy, is composed of three to four glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP3 is essential for zona matrix formation. In pig, does not seem to have a sperm-binding activity by itself but may increase sperm-binding activity of ZPB.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processFertilization

Names & Taxonomyi

Protein namesi
Recommended name:
Zona pellucida sperm-binding protein 3
Alternative name(s):
Sperm receptor
Zona pellucida glycoprotein 3
Short name:
Zp-3
Zona pellucida glycoprotein 3-beta
Short name:
Zp-3-beta
Short name:
Zp3-beta
Zona pellucida protein C
Cleaved into the following chain:
Gene namesi
Name:ZP3
Synonyms:ZP3B, ZPC
OrganismiSus scrofa (Pig)
Taxonomic identifieri9823 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaSuinaSuidaeSus
Proteomesi
  • UP000008227 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 381ExtracellularSequence analysisAdd BLAST359
Transmembranei382 – 402HelicalSequence analysisAdd BLAST21
Topological domaini403 – 421CytoplasmicSequence analysisAdd BLAST19

Keywords - Cellular componenti

Cell membrane, Extracellular matrix, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 221 PublicationAdd BLAST22
ChainiPRO_000004171723 – 332Zona pellucida sperm-binding protein 3Add BLAST310
ChainiPRO_000030457323 – ?Processed zona pellucida sperm-binding protein 3
PropeptideiPRO_0000041718333 – 421Removed in mature form1 PublicationAdd BLAST89

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei23Pyrrolidone carboxylic acid1 Publication1
Disulfide bondi45 ↔ 139By similarity
Disulfide bondi77 ↔ 98By similarity
GlycosylationiCAR_000151124N-linked (GlcNAc...) asparagine1 Publication1
GlycosylationiCAR_000152146N-linked (GlcNAc...) asparagine1 Publication1
Glycosylationi155O-linked (GalNAc...) threonine1 Publication1
Glycosylationi161O-linked (GalNAc...) threonine1 Publication1
Glycosylationi162O-linked (GalNAc...) threonine1 Publication1
Glycosylationi179N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi216 ↔ 281By similarity
Disulfide bondi238 ↔ 299By similarity
GlycosylationiCAR_000153271N-linked (GlcNAc...) asparagine1 Publication1

Post-translational modificationi

Proteolytically cleaved before the transmembrane segment to yield the secreted ectodomain incorporated in the zona pellucida.1 Publication
O-glycosylated; removal of O-linked glycans may play an important role in the post-fertilization block to polyspermy.By similarity
All cysteine residues are involved in disulfide bonds.

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein, Pyrrolidone carboxylic acid

Proteomic databases

PaxDbiP42098
PRIDEiP42098

PTM databases

GlyConnecti632
636
iPTMnetiP42098
UniCarbKBiP42098

Expressioni

Tissue specificityi

Oocytes.

Interactioni

Subunit structurei

Polymers of ZP2 and ZP3 organized into long filaments cross-linked by ZP1 homodimers. Interacts with ZP1 and ZP2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9823.ENSSSCP00000008213

Structurei

3D structure databases

ProteinModelPortaliP42098
SMRiP42098
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini44 – 306ZPPROSITE-ProRule annotationAdd BLAST263

Domaini

The ZP domain is involved in the polymerization of the ZP proteins to form the zona pellucida.

Sequence similaritiesi

Belongs to the ZP domain family. ZPC subfamily.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II9V Eukaryota
ENOG410YXE1 LUCA
HOGENOMiHOG000220813
HOVERGENiHBG007985
InParanoidiP42098
KOiK19928

Family and domain databases

InterProiView protein in InterPro
IPR001507 ZP_dom
IPR017977 ZP_dom_CS
PfamiView protein in Pfam
PF00100 Zona_pellucida, 1 hit
PRINTSiPR00023 ZPELLUCIDA
SMARTiView protein in SMART
SM00241 ZP, 1 hit
PROSITEiView protein in PROSITE
PS00682 ZP_1, 1 hit
PS51034 ZP_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P42098-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPSWRFFVC FLLWGGTELC SPQPVWQDEG QRLRPSKPPT VMVECQEAQL
60 70 80 90 100
VVIVSKDLFG TGKLIRPADL SLGPAKCEPL VSQDTDAVVR FEVGLHECGS
110 120 130 140 150
SLQVTDDALV YSTFLRHDPR PAGNLSILRT NRAEVPIECH YPRQGNVSSW
160 170 180 190 200
AILPTWVPFR TTVFSEEKLV FSLRLMEENW SAEKMTPTFQ LGDRAHLQAQ
210 220 230 240 250
VHTGSHVPLR LFVDHCVATL TPDWNTSPSH TIVDFHGCLV DGLTEASSAF
260 270 280 290 300
KAPRPGPETL QFTVDVFHFA NDSRNTIYIT CHLKVTPADR VPDQLNKACS
310 320 330 340 350
FSKSSNRWSP VEGPAVICRC CHKGQCGTPS LSRKLSMPKR QSAPRSRRHV
360 370 380 390 400
TDEADVTVGP LIFLGKTSDH GVEGSTSSPT SVMVGLGLAT VVTLTLATIV
410 420
LGVPRRRRAA AHLVCPVSAS Q
Length:421
Mass (Da):46,239
Last modified:November 1, 1995 - v1
Checksum:iDC97D760E985213A
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti101Missing in BAA08093 (Ref. 2) Curated1
Sequence conflicti107D → V in BAA08093 (Ref. 2) Curated1
Sequence conflicti163 – 164VF → GV in BAA08093 (Ref. 2) Curated2
Sequence conflicti404P → A in BAA08093 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L22169 mRNA Translation: AAA31145.1
D45065 mRNA Translation: BAA08093.1
PIRiS70433
RefSeqiNP_999058.1, NM_213893.1
UniGeneiSsc.80359

Genome annotation databases

GeneIDi396927
KEGGissc:396927

Similar proteinsi

Entry informationi

Entry nameiZP3_PIG
AccessioniPrimary (citable) accession number: P42098
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 25, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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