Reviewed, UniProtKB/Swiss-Prot P42095 (RECO_BACSU)

Names and origin

Protein names

Recommended name:
    DNA repair protein recO
Alternative name(s):
    Recombination protein O

Gene names

Name:	recO
Synonyms:	yqfI, yqxN
Ordered Locus Names:	BSU25280

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Plays a role in DNA double-stranded break repair. It seems to be involved in an recBC-independent recombinational process of DNA repair. It may act with recF and recR. Is recruited to repair centers, foci that are the site of double-strand break(s) after recN and before recF; may actively recruit recF. HAMAP MF_00201
Subunit structure	Recruited to foci following DNA damage; probably interacts with recF. HAMAP MF_00201
Subcellular location	Cytoplasm › nucleoid. Note: Cytoplasmically located in untreated cells. Recruited to foci following treatment with DNA damaging agents; these foci are presumably the breaks themselves. They are almost always located within nucleoids. Ref.6
Sequence similarities	Belongs to the recO family.

Ontologies

Keywords
Biological process	DNA damage DNA recombination DNA repair
Cellular component	Cytoplasm
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA recombination Inferred from electronic annotation. Source: HAMAP double-strand break repair Ref.6 Inferred from direct assay. Source: UniProtKB
Cellular component	bacterial nucleoid Ref.6 Inferred from direct assay. Source: UniProtKB cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	DNA repair protein recO HAMAP MF_00201		PRO_0000204932
Experimental info
	Sequence conflict	122	1	L → M in BAA12483. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P42095-1 [UniParc]. Last modified June 16, 2009. Version 3. Checksum: 01E9207DEF476276	FASTA	255	29,328
	10 20 30 40 50 60 MLTKCEGIVL RTNDYGETNK IVTLLTREHG KIGVMARGAK KPNSRLSAVS QPFLYGSFLM 70 80 90 100 110 120 QKTSGLGTLQ QGEMILSMRG IREDLFLTAY AAYVAELVDR GTEEKKPNPY LFEFILESLK 130 140 150 160 170 180 QLNEGTDPDV ITFIVQMKML GVMGLYPELN HCVHCKSQDG TFHFSVRDNG FICHRCFEKD 190 200 210 220 230 240 PYRIPIKPQT ARLLRLFYYF DLSRLGNVSL KEETKAELKQ VIDLYYEEYS GLYLKSKRFL 250 DQMESMKHLM GENKS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the Bacillus subtilis genome containing the skin element and many sporulation genes." Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M., Kobayashi Y. Microbiology 142:3103-3111(1996) [PubMed: 8969508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / JH642.
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 122.
[4]	Johnstone B.H., Torrance P.D., Maslesa-Galic S., Zarimani A.I., Powell B.S., Court D.L., Simons R.W. Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-114. Strain: 168.
[5]	"Analysis of the Bacillus subtilis recO gene: recO forms part of the RecFLOR function." Fernandez S., Kobayashi Y., Ogasawara N., Alonso J.C. Mol. Gen. Genet. 261:567-573(1999) [PubMed: 10323239] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Visualization of DNA double-strand break repair in live bacteria reveals dynamic recruitment of Bacillus subtilis RecF, RecO and RecN proteins to distinct sites on the nucleoids." Kidane D., Sanchez H., Alonso J.C., Graumann P.L. Mol. Microbiol. 52:1627-1639(2004) [PubMed: 15186413] [Abstract] Cited for: SUBCELLULAR LOCATION, TEMPORAL ORDER OF DNA DOUBLE-STRAND BREAK RECRUITMENT. Strain: 168 / YB886.
[7]	"Dynamic formation of RecA filaments at DNA double strand break repair centers in live cells." Kidane D., Graumann P.L. J. Cell Biol. 170:357-366(2005) [PubMed: 16061691] [Abstract] Cited for: RECRUITMENT OF RECA. Strain: 168 / YB886.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D84432 Genomic DNA. Translation: BAA12483.1. AL009126 Genomic DNA. Translation: CAB14457.2. U18532 Genomic DNA. Translation: AAB59995.1.
PIR	H69968.
RefSeq	NP_390406.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	937881.
GenomeReviews	Gene locus BSU25280 in contig AL009126_GR.
KEGG	bsu:BSU25280.
NMPDR	fig|224308.1.peg.2531.
Organism-specific databases
SubtiList	BG11189. recO. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P42095.
OMA	YSGLYLK.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2524-MON.
Family and domain databases
HAMAP	MF_00201. [Tree]
InterPro	IPR003717. RecO. [Graphical view]
Pfam	PF02565. RecO. 1 hit. [Graphical view]
TIGRFAMs	TIGR00613. reco. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RECO_BACSU

Accession

Primary (citable) accession number: P42095

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 16, 2009
Last modified:	November 3, 2009
This is version 62 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)