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Protein

Imidazolonepropionase

Gene

hutI

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

(S)-3-(5-oxo-4,5-dihydro-3H-imidazol-4-yl)propanoate + H2O = N-formimidoyl-L-glutamate + H+.UniRule annotation

Cofactori

Zn2+, Fe2+Note: Binds 1 zinc or iron ion per subunit.

Pathwayi: L-histidine degradation into L-glutamate

This protein is involved in step 3 of the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine.UniRule annotation
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Histidine ammonia-lyase (hutH)
  2. Urocanate hydratase (hutU)
  3. Imidazolonepropionase (hutI)
This subpathway is part of the pathway L-histidine degradation into L-glutamate, which is itself part of Amino-acid degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N-formimidoyl-L-glutamate from L-histidine, the pathway L-histidine degradation into L-glutamate and in Amino-acid degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi80Zinc or iron1
Metal bindingi82Zinc or iron1
Binding sitei89Substrate1
Binding sitei102Substrate1
Binding sitei152Substrate1
Binding sitei185Substrate1
Metal bindingi249Zinc or iron1
Binding sitei252Substrate1
Metal bindingi324Zinc or iron1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Histidine metabolism

Keywords - Ligandi

Iron, Metal-binding, Zinc

Enzyme and pathway databases

BioCyciBSUB:BSU39370-MONOMER.
MetaCyc:HUTIBACSU-MONOMER.
BRENDAi3.5.2.7. 658.
UniPathwayiUPA00379; UER00551.

Protein family/group databases

MEROPSiM38.980.

Names & Taxonomyi

Protein namesi
Recommended name:
ImidazolonepropionaseUniRule annotation (EC:3.5.2.7UniRule annotation)
Alternative name(s):
Imidazolone-5-propionate hydrolaseUniRule annotation
Gene namesi
Name:hutIUniRule annotation
Ordered Locus Names:BSU39370
ORF Names:EE57B
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001609441 – 421ImidazolonepropionaseAdd BLAST421

Proteomic databases

PaxDbiP42084.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021246.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 15Combined sources12
Helixi26 – 29Combined sources4
Beta strandi35 – 43Combined sources9
Beta strandi46 – 52Combined sources7
Turni53 – 58Combined sources6
Beta strandi60 – 66Combined sources7
Beta strandi71 – 74Combined sources4
Beta strandi76 – 78Combined sources3
Helixi90 – 92Combined sources3
Helixi93 – 97Combined sources5
Helixi102 – 107Combined sources6
Helixi112 – 121Combined sources10
Helixi124 – 140Combined sources17
Beta strandi143 – 149Combined sources7
Helixi156 – 172Combined sources17
Beta strandi173 – 186Combined sources14
Helixi189 – 191Combined sources3
Helixi195 – 203Combined sources9
Helixi206 – 211Combined sources6
Beta strandi216 – 222Combined sources7
Helixi229 – 241Combined sources13
Beta strandi245 – 250Combined sources6
Beta strandi252 – 254Combined sources3
Helixi258 – 264Combined sources7
Beta strandi268 – 272Combined sources5
Helixi278 – 287Combined sources10
Beta strandi290 – 293Combined sources4
Helixi295 – 300Combined sources6
Helixi309 – 314Combined sources6
Beta strandi319 – 321Combined sources3
Turni327 – 329Combined sources3
Helixi335 – 345Combined sources11
Helixi350 – 356Combined sources7
Helixi359 – 364Combined sources6
Turni368 – 370Combined sources3
Beta strandi382 – 389Combined sources8
Helixi392 – 395Combined sources4
Beta strandi402 – 407Combined sources6
Beta strandi410 – 414Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BB0X-ray2.00A/B1-421[»]
2G3FX-ray2.00A/B1-421[»]
ProteinModelPortaliP42084.
SMRiP42084.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP42084.

Family & Domainsi

Sequence similaritiesi

Belongs to the HutI family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CI2. Bacteria.
COG1228. LUCA.
HOGENOMiHOG000218460.
InParanoidiP42084.
KOiK01468.
OMAiDHCTHLT.
PhylomeDBiP42084.

Family and domain databases

CDDicd01296. Imidazolone-5PH. 1 hit.
Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_00372. HutI. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01224. hutI. 1 hit.

Sequencei

Sequence statusi: Complete.

P42084-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPKQIDTILI NIGQLLTMES SGPRAGKSMQ DLHVIEDAVV GIHEQKIVFA
60 70 80 90 100
GQKGAEAGYE ADEIIDCSGR LVTPGLVDPH THLVFGGSRE KEMNLKLQGI
110 120 130 140 150
SYLDILAQGG GILSTVKDTR AASEEELLQK AHFHLQRMLS YGTTTAEVKS
160 170 180 190 200
GYGLEKETEL KQLRVAKKLH ESQPVDLVST FMGAHAIPPE YQNDPDDFLD
210 220 230 240 250
QMLSLLPEIK EQELASFADI FTETGVFTVS QSRRYLQKAA EAGFGLKIHA
260 270 280 290 300
DEIDPLGGAE LAGKLKAVSA DHLVGTSDEG IKKLAEAGTI AVLLPGTTFY
310 320 330 340 350
LGKSTYARAR AMIDEGVCVS LATDFNPGSS PTENIQLIMS IAALHLKMTA
360 370 380 390 400
EEIWHAVTVN AAYAIGKGEE AGQLKAGRSA DLVIWQAPNY MYIPYHYGVN
410 420
HVHQVMKNGT IVVNREGAIL G
Length:421
Mass (Da):45,564
Last modified:November 1, 1995 - v1
Checksum:iA0E1893961745771
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31856 Genomic DNA. Translation: BAA06642.1.
AL009126 Genomic DNA. Translation: CAB15973.1.
PIRiD69643.
RefSeqiNP_391816.1. NC_000964.3.
WP_003244327.1. NZ_JNCM01000034.1.

Genome annotation databases

EnsemblBacteriaiCAB15973; CAB15973; BSU39370.
GeneIDi937531.
KEGGibsu:BSU39370.
PATRICi18979938. VBIBacSub10457_4131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31856 Genomic DNA. Translation: BAA06642.1.
AL009126 Genomic DNA. Translation: CAB15973.1.
PIRiD69643.
RefSeqiNP_391816.1. NC_000964.3.
WP_003244327.1. NZ_JNCM01000034.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2BB0X-ray2.00A/B1-421[»]
2G3FX-ray2.00A/B1-421[»]
ProteinModelPortaliP42084.
SMRiP42084.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100021246.

Protein family/group databases

MEROPSiM38.980.

Proteomic databases

PaxDbiP42084.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAB15973; CAB15973; BSU39370.
GeneIDi937531.
KEGGibsu:BSU39370.
PATRICi18979938. VBIBacSub10457_4131.

Phylogenomic databases

eggNOGiENOG4105CI2. Bacteria.
COG1228. LUCA.
HOGENOMiHOG000218460.
InParanoidiP42084.
KOiK01468.
OMAiDHCTHLT.
PhylomeDBiP42084.

Enzyme and pathway databases

UniPathwayiUPA00379; UER00551.
BioCyciBSUB:BSU39370-MONOMER.
MetaCyc:HUTIBACSU-MONOMER.
BRENDAi3.5.2.7. 658.

Miscellaneous databases

EvolutionaryTraceiP42084.

Family and domain databases

CDDicd01296. Imidazolone-5PH. 1 hit.
Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_00372. HutI. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR005920. HutI.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
[Graphical view]
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 1 hit.
TIGRFAMsiTIGR01224. hutI. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHUTI_BACSU
AccessioniPrimary (citable) accession number: P42084
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.