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P42082

- CD86_MOUSE

UniProt

P42082 - CD86_MOUSE

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Protein

T-lymphocyte activation antigen CD86

Gene

Cd86

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.

GO - Biological processi

  1. aging Source: Ensembl
  2. B cell activation Source: MGI
  3. cellular response to cytokine stimulus Source: Ensembl
  4. cellular response to lipopolysaccharide Source: Ensembl
  5. cellular response to metal ion Source: Ensembl
  6. defense response to virus Source: MGI
  7. myeloid dendritic cell differentiation Source: Ensembl
  8. negative regulation of T cell anergy Source: Ensembl
  9. positive regulation of activated T cell proliferation Source: Ensembl
  10. positive regulation of T cell proliferation Source: MGI
  11. response to drug Source: Ensembl
  12. response to interferon-gamma Source: Ensembl
  13. response to yeast Source: Ensembl
  14. T cell proliferation involved in immune response Source: Ensembl
  15. toll-like receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_204081. CD28 co-stimulation.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
T-lymphocyte activation antigen CD86
Alternative name(s):
Activation B7-2 antigen
Early T-cell costimulatory molecule 1
Short name:
ETC-1
CD_antigen: CD86
Gene namesi
Name:Cd86
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 16

Organism-specific databases

MGIiMGI:101773. Cd86.

Subcellular locationi

GO - Cellular componenti

  1. external side of plasma membrane Source: MGI
  2. extracellular vesicular exosome Source: Ensembl
  3. integral component of membrane Source: UniProtKB-KW
  4. intracellular membrane-bounded organelle Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence AnalysisAdd
BLAST
Chaini24 – 309286T-lymphocyte activation antigen CD86PRO_0000014551Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi33 – 331N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi40 ↔ 110PROSITE-ProRule annotation
Glycosylationi47 – 471N-linked (GlcNAc...)Sequence Analysis
Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
Glycosylationi135 – 1351N-linked (GlcNAc...)Sequence Analysis
Glycosylationi146 – 1461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi154 – 1541N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi157 ↔ 216PROSITE-ProRule annotation
Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
Glycosylationi190 – 1901N-linked (GlcNAc...)Sequence Analysis
Glycosylationi231 – 2311N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PRIDEiP42082.

PTM databases

PhosphoSiteiP42082.

Expressioni

Tissue specificityi

Expressed on activated B-cells.

Gene expression databases

BgeeiP42082.
CleanExiMM_CD86.
ExpressionAtlasiP42082. baseline and differential.
GenevestigatoriP42082.

Interactioni

Subunit structurei

Homodimer. Interacts with MARCH8 (By similarity).By similarity

Protein-protein interaction databases

BioGridi198617. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP42082.
SMRiP42082. Positions 26-204.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 244221ExtracellularSequence AnalysisAdd
BLAST
Topological domaini266 – 30944CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei245 – 26521HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini33 – 12896Ig-like V-typeAdd
BLAST
Domaini150 – 22374Ig-like C2-typeAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG79621.
HOVERGENiHBG004093.
InParanoidiP42082.
KOiK05413.
OMAiIANFSEP.
OrthoDBiEOG70PC0G.
PhylomeDBiP42082.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00406. IGv. 1 hit.
[Graphical view]
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P42082-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPRCTMGLA ILIFVTVLLI SDAVSVETQA YFNGTAYLPC PFTKAQNISL
60 70 80 90 100
SELVVFWQDQ QKLVLYEHYL GTEKLDSVNA KYLGRTSFDR NNWTLRLHNV
110 120 130 140 150
QIKDMGSYDC FIQKKPPTGS IILQQTLTEL SVIANFSEPE IKLAQNVTGN
160 170 180 190 200
SGINLTCTSK QGHPKPKKMY FLITNSTNEY GDNMQISQDN VTELFSISNS
210 220 230 240 250
LSLSFPDGVW HMTVVCVLET ESMKISSKPL NFTQEFPSPQ TYWKEITASV
260 270 280 290 300
TVALLLVMLL IIVCHKKPNQ PSRPSNTASK LERDSNADRE TINLKELEPQ

IASAKPNAE
Length:309
Mass (Da):34,666
Last modified:November 1, 1995 - v1
Checksum:i8F58DCD1FB81D5EA
GO
Isoform 2 (identifier: P42082-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Show »
Length:303
Mass (Da):33,962
Checksum:i92526096A8C6F249
GO

Sequence cautioni

The sequence AAB30744.2 differs from that shown. Reason: Erroneous initiation.

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 66Missing in isoform 2. CuratedVSP_023125

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25606 mRNA. Translation: AAA79770.1.
S70108 mRNA. Translation: AAB30744.2. Different initiation.
U39456
, U39459, U39461, U39462, U39463, U39464, U39465, U39466 Genomic DNA. Translation: AAC52334.1.
U39456
, U39461, U39462, U39463, U39464, U39465, U39466 Genomic DNA. Translation: AAC52336.1.
CCDSiCCDS28155.1. [P42082-1]
PIRiI49522.
RefSeqiNP_062261.3. NM_019388.3. [P42082-1]
XP_006521805.1. XM_006521742.1. [P42082-2]
UniGeneiMm.1452.

Genome annotation databases

EnsembliENSMUST00000089620; ENSMUSP00000087047; ENSMUSG00000022901. [P42082-1]
GeneIDi12524.
KEGGimmu:12524.
UCSCiuc007zcq.2. mouse. [P42082-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25606 mRNA. Translation: AAA79770.1 .
S70108 mRNA. Translation: AAB30744.2 . Different initiation.
U39456
, U39459 , U39461 , U39462 , U39463 , U39464 , U39465 , U39466 Genomic DNA. Translation: AAC52334.1 .
U39456
, U39461 , U39462 , U39463 , U39464 , U39465 , U39466 Genomic DNA. Translation: AAC52336.1 .
CCDSi CCDS28155.1. [P42082-1 ]
PIRi I49522.
RefSeqi NP_062261.3. NM_019388.3. [P42082-1 ]
XP_006521805.1. XM_006521742.1. [P42082-2 ]
UniGenei Mm.1452.

3D structure databases

ProteinModelPortali P42082.
SMRi P42082. Positions 26-204.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 198617. 1 interaction.

PTM databases

PhosphoSitei P42082.

Proteomic databases

PRIDEi P42082.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000089620 ; ENSMUSP00000087047 ; ENSMUSG00000022901 . [P42082-1 ]
GeneIDi 12524.
KEGGi mmu:12524.
UCSCi uc007zcq.2. mouse. [P42082-1 ]

Organism-specific databases

CTDi 942.
MGIi MGI:101773. Cd86.

Phylogenomic databases

eggNOGi NOG79621.
HOVERGENi HBG004093.
InParanoidi P42082.
KOi K05413.
OMAi IANFSEP.
OrthoDBi EOG70PC0G.
PhylomeDBi P42082.
TreeFami TF331083.

Enzyme and pathway databases

Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_204081. CD28 co-stimulation.
REACT_226151. CD28 dependent PI3K/Akt signaling.
REACT_226341. PIP3 activates AKT signaling.

Miscellaneous databases

NextBioi 281542.
PROi P42082.
SOURCEi Search...

Gene expression databases

Bgeei P42082.
CleanExi MM_CD86.
ExpressionAtlasi P42082. baseline and differential.
Genevestigatori P42082.

Family and domain databases

Gene3Di 2.60.40.10. 2 hits.
InterProi IPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
IPR003596. Ig_V-set_subgr.
[Graphical view ]
Pfami PF07686. V-set. 1 hit.
[Graphical view ]
SMARTi SM00406. IGv. 1 hit.
[Graphical view ]
PROSITEi PS50835. IG_LIKE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Murine B7-2, an alternative CTLA4 counter-receptor that costimulates T cell proliferation and interleukin 2 production."
    Freeman G.J., Borriello F., Hodes R.J., Reiser H., Gribben J.G., Ng J.W., Kim J., Goldberg J.M., Hathcock K., Laszlo G., Lombard L.A., Wang S., Gray G.S., Nadler L.M., Sharpe A.H.
    J. Exp. Med. 178:2185-2192(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Molecular cloning and expression of early T cell costimulatory molecule-1 and its characterization as B7-2 molecule."
    Chen C., Gault A., Shen L., Nabavi N.
    J. Immunol. 152:4929-4936(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "Differential expression of alternate mB7-2 transcripts."
    Borriello F., Oliveros J., Freeman G.J., Nadler L.M., Sharpe A.H.
    J. Immunol. 155:5490-5497(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: 129.

Entry informationi

Entry nameiCD86_MOUSE
AccessioniPrimary (citable) accession number: P42082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3