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Protein

T-lymphocyte activation antigen CD86

Gene

Cd86

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.

GO - Biological processi

  • adaptive immune response Source: UniProtKB-KW
  • aging Source: Ensembl
  • B cell activation Source: MGI
  • cellular response to cytokine stimulus Source: Ensembl
  • cellular response to lipopolysaccharide Source: MGI
  • cellular response to metal ion Source: Ensembl
  • defense response to virus Source: MGI
  • myeloid dendritic cell differentiation Source: Ensembl
  • negative regulation of T cell anergy Source: Ensembl
  • positive regulation of activated T cell proliferation Source: Ensembl
  • positive regulation of T cell proliferation Source: MGI
  • response to drug Source: Ensembl
  • response to interferon-gamma Source: Ensembl
  • response to yeast Source: Ensembl
  • T cell proliferation involved in immune response Source: Ensembl
  • toll-like receptor 3 signaling pathway Source: UniProtKB
  • toll-like receptor signaling pathway Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Keywords - Biological processi

Adaptive immunity, Immunity

Enzyme and pathway databases

ReactomeiR-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-389356. CD28 co-stimulation.
R-MMU-389357. CD28 dependent PI3K/Akt signaling.
R-MMU-389359. CD28 dependent Vav1 pathway.
R-MMU-389513. CTLA4 inhibitory signaling.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
T-lymphocyte activation antigen CD86
Alternative name(s):
Activation B7-2 antigen
Early T-cell costimulatory molecule 1
Short name:
ETC-1
CD_antigen: CD86
Gene namesi
Name:Cd86
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:101773. Cd86.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 244ExtracellularSequence analysisAdd BLAST221
Transmembranei245 – 265HelicalSequence analysisAdd BLAST21
Topological domaini266 – 309CytoplasmicSequence analysisAdd BLAST44

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000001455124 – 309T-lymphocyte activation antigen CD86Add BLAST286

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi33N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi40 ↔ 110PROSITE-ProRule annotation
Glycosylationi47N-linked (GlcNAc...)Sequence analysis1
Glycosylationi92N-linked (GlcNAc...)Sequence analysis1
Glycosylationi135N-linked (GlcNAc...)Sequence analysis1
Glycosylationi146N-linked (GlcNAc...)Sequence analysis1
Glycosylationi154N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi157 ↔ 216PROSITE-ProRule annotation
Glycosylationi175N-linked (GlcNAc...)Sequence analysis1
Glycosylationi190N-linked (GlcNAc...)Sequence analysis1
Glycosylationi231N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

PaxDbiP42082.
PeptideAtlasiP42082.
PRIDEiP42082.

PTM databases

iPTMnetiP42082.
PhosphoSitePlusiP42082.
SwissPalmiP42082.

Expressioni

Tissue specificityi

Expressed on activated B-cells.

Gene expression databases

BgeeiENSMUSG00000022901.
CleanExiMM_CD86.
ExpressionAtlasiP42082. baseline and differential.
GenevisibleiP42082. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with MARCH8 (By similarity).By similarity

Protein-protein interaction databases

BioGridi198617. 1 interactor.
STRINGi10090.ENSMUSP00000087047.

Structurei

3D structure databases

ProteinModelPortaliP42082.
SMRiP42082.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini33 – 128Ig-like V-typeAdd BLAST96
Domaini150 – 223Ig-like C2-typeAdd BLAST74

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IYP1. Eukaryota.
ENOG410ZD66. LUCA.
GeneTreeiENSGT00720000108819.
HOVERGENiHBG004093.
InParanoidiP42082.
KOiK05413.
OMAiTAYLPCP.
OrthoDBiEOG091G0QVY.
PhylomeDBiP42082.
TreeFamiTF331083.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P42082-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDPRCTMGLA ILIFVTVLLI SDAVSVETQA YFNGTAYLPC PFTKAQNISL
60 70 80 90 100
SELVVFWQDQ QKLVLYEHYL GTEKLDSVNA KYLGRTSFDR NNWTLRLHNV
110 120 130 140 150
QIKDMGSYDC FIQKKPPTGS IILQQTLTEL SVIANFSEPE IKLAQNVTGN
160 170 180 190 200
SGINLTCTSK QGHPKPKKMY FLITNSTNEY GDNMQISQDN VTELFSISNS
210 220 230 240 250
LSLSFPDGVW HMTVVCVLET ESMKISSKPL NFTQEFPSPQ TYWKEITASV
260 270 280 290 300
TVALLLVMLL IIVCHKKPNQ PSRPSNTASK LERDSNADRE TINLKELEPQ

IASAKPNAE
Length:309
Mass (Da):34,666
Last modified:November 1, 1995 - v1
Checksum:i8F58DCD1FB81D5EA
GO
Isoform 2 (identifier: P42082-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-6: Missing.

Show »
Length:303
Mass (Da):33,962
Checksum:i92526096A8C6F249
GO

Sequence cautioni

The sequence AAB30744 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0231251 – 6Missing in isoform 2. Curated6

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25606 mRNA. Translation: AAA79770.1.
S70108 mRNA. Translation: AAB30744.2. Different initiation.
U39456
, U39459, U39461, U39462, U39463, U39464, U39465, U39466 Genomic DNA. Translation: AAC52334.1.
U39456
, U39461, U39462, U39463, U39464, U39465, U39466 Genomic DNA. Translation: AAC52336.1.
CCDSiCCDS28155.1. [P42082-1]
PIRiI49522.
RefSeqiNP_062261.3. NM_019388.3. [P42082-1]
XP_011244114.1. XM_011245812.1. [P42082-2]
UniGeneiMm.1452.

Genome annotation databases

EnsembliENSMUST00000089620; ENSMUSP00000087047; ENSMUSG00000022901. [P42082-1]
GeneIDi12524.
KEGGimmu:12524.
UCSCiuc007zcq.2. mouse. [P42082-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25606 mRNA. Translation: AAA79770.1.
S70108 mRNA. Translation: AAB30744.2. Different initiation.
U39456
, U39459, U39461, U39462, U39463, U39464, U39465, U39466 Genomic DNA. Translation: AAC52334.1.
U39456
, U39461, U39462, U39463, U39464, U39465, U39466 Genomic DNA. Translation: AAC52336.1.
CCDSiCCDS28155.1. [P42082-1]
PIRiI49522.
RefSeqiNP_062261.3. NM_019388.3. [P42082-1]
XP_011244114.1. XM_011245812.1. [P42082-2]
UniGeneiMm.1452.

3D structure databases

ProteinModelPortaliP42082.
SMRiP42082.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198617. 1 interactor.
STRINGi10090.ENSMUSP00000087047.

PTM databases

iPTMnetiP42082.
PhosphoSitePlusiP42082.
SwissPalmiP42082.

Proteomic databases

PaxDbiP42082.
PeptideAtlasiP42082.
PRIDEiP42082.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000089620; ENSMUSP00000087047; ENSMUSG00000022901. [P42082-1]
GeneIDi12524.
KEGGimmu:12524.
UCSCiuc007zcq.2. mouse. [P42082-1]

Organism-specific databases

CTDi942.
MGIiMGI:101773. Cd86.

Phylogenomic databases

eggNOGiENOG410IYP1. Eukaryota.
ENOG410ZD66. LUCA.
GeneTreeiENSGT00720000108819.
HOVERGENiHBG004093.
InParanoidiP42082.
KOiK05413.
OMAiTAYLPCP.
OrthoDBiEOG091G0QVY.
PhylomeDBiP42082.
TreeFamiTF331083.

Enzyme and pathway databases

ReactomeiR-MMU-1257604. PIP3 activates AKT signaling.
R-MMU-389356. CD28 co-stimulation.
R-MMU-389357. CD28 dependent PI3K/Akt signaling.
R-MMU-389359. CD28 dependent Vav1 pathway.
R-MMU-389513. CTLA4 inhibitory signaling.
R-MMU-6811558. PI5P, PP2A and IER3 Regulate PI3K/AKT Signaling.

Miscellaneous databases

PROiP42082.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022901.
CleanExiMM_CD86.
ExpressionAtlasiP42082. baseline and differential.
GenevisibleiP42082. MM.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
InterProiIPR007110. Ig-like_dom.
IPR013783. Ig-like_fold.
IPR013106. Ig_V-set.
[Graphical view]
PfamiPF07686. V-set. 1 hit.
[Graphical view]
SMARTiSM00406. IGv. 1 hit.
[Graphical view]
SUPFAMiSSF48726. SSF48726. 1 hit.
PROSITEiPS50835. IG_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCD86_MOUSE
AccessioniPrimary (citable) accession number: P42082
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.